BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,857,759	G→T	6.4%	E84D (GAG→GAT) ‡	PP_5134 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,857,759	0	G	T	6.4%	430.5 / 11.3	135	E84D (GAG→GAT) ‡	PP_5134	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (65/61); new base T (5/4); total (70/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 142 total aligned reads displayed.

TGTCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGTGCTGGCC  >  NC_002947/5857611‑5857906

tgtCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAgc                                                                                                                                                    <  1:2095555/150‑1 (MQ=255)

  tcgcACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAgcgc                                                                                                                                                  >  1:2403764/4‑150 (MQ=255)

  tCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAgcgc                                                                                                                                                  <  1:1017368/150‑1 (MQ=255)

  tCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCGTGAACGCCTTGAGCGC‑CCAGCCGAgcgc                                                                                                                                                  >  2:1598347/1‑150 (MQ=255)

    ccACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCt                                                                                                                                                >  1:835499/1‑150 (MQ=255)

     cACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTg                                                                                                                                               >  1:585272/1‑150 (MQ=255)

     cACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTg                                                                                                                                               >  2:443327/1‑150 (MQ=255)

      aCCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGT‑CCAGGCGCTCGCCTgg                                                                                                                                              <  2:2249281/150‑1 (MQ=255)

       ccGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGa                                                                                                                                             <  1:576668/150‑1 (MQ=255)

        cGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGCTCGCCTgggc                                                                                                                                            <  2:1959771/150‑3 (MQ=255)

        cGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGAc                                                                                                                                            <  2:1830501/150‑1 (MQ=255)

         gCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCCGGCGAGCGCCTGGACg                                                                                                                                           <  1:1891423/150‑1 (MQ=255)

              aGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCt                                                                                                                                      >  1:466139/1‑150 (MQ=255)

               gCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGcc                                                                                                                                        <  1:1047293/147‑1 (MQ=255)

                     ctCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACgg                                                                                                                                          >  2:2091257/1‑139 (MQ=255)

                     ctCGCCATGTGGAAGGTGCACGGCTGTCGCTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGc                                                                                                                               <  1:1839761/150‑1 (MQ=255)

                      tCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCt                                                                                                                              >  1:134750/1‑150 (MQ=255)

                        gCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGAGTACCTGGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTgg                                                                                                                            >  2:572595/1‑150 (MQ=255)

                         ccATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGa                                                                                                                           <  2:134750/150‑1 (MQ=255)

                              tgGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGtt                                                                                                                      <  2:585272/150‑1 (MQ=255)

                               ggAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTTGAGCGC‑CCGGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGttt                                                                                                                     >  1:531070/1‑150 (MQ=255)

                                gAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGtttt                                                                                                                    >  2:840501/1‑150 (MQ=255)

                                      gCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAg                                                                                                              <  1:235041/150‑1 (MQ=255)

                                      gCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAg                                                                                                              <  2:835499/150‑1 (MQ=255)

                                      gCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAg                                                                                                              <  2:2403764/150‑1 (MQ=255)

                                        aCGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTg                                                                                                                                               >  2:902522/1‑115 (MQ=255)

                                        aCGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTg                                                                                                                                               <  1:902522/115‑1 (MQ=255)

                                        aCGGCTGTCGGTGGACGGTGAACCTTTGCTGAAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCCTTTTCTGCAggg                                                                                                            >  2:582372/1‑150 (MQ=255)

                                            cTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCt                                                                                                                              <  2:1822997/128‑1 (MQ=255)

                                            cTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGc                                                                                                        >  2:512241/1‑150 (MQ=255)

                                                       cGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCGGGGCAGCGAAGCGCGCCa                                                                                             >  1:1573685/1‑150 (MQ=255)

                                                        ggTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCaa                                                                                            <  2:1437221/150‑1 (MQ=255)

                                                          tGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACg                                                                                          >  2:472401/1‑150 (MQ=255)

                                                          tGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTCGAACGCCTGGAGCGT‑CCAGGCGCTCGCCTgggcgc                                                                                                                                          >  2:397078/1‑98 (MQ=255)

                                                          tGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTCGAACGCCTGGAGCGT‑CCAGGCGCTCGCCTGGGCgc                                                                                                                                          <  1:397078/102‑2 (MQ=255)

                                                           gAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGc                                                                                         <  2:1993561/150‑1 (MQ=255)

                                                              ccTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTc                                                                                      <  2:680754/150‑1 (MQ=255)

                                                                tttGGTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGa                                                                                    <  1:407566/150‑1 (MQ=255)

                                                                tttGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCGTCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCTAACGCTTCGa                                                                                    >  1:2297066/1‑150 (MQ=255)

                                                                 ttGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAg                                                                                   <  2:1934425/150‑1 (MQ=255)

                                                                   gCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCa                                                                                 <  2:315099/150‑1 (MQ=255)

                                                                   gCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTCATGCAGGCCAGCGAAGCGCGCCAACGCTTCGAGCa                                                                                 <  1:55179/150‑1 (MQ=255)

                                                                       gAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCaggag                                                                             <  1:2273722/150‑1 (MQ=255)

                                                                            gcaCGACACGCCCGTCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGACCGCCTGGCGCGC‑CCAGGCGAGCGCCTGCACGGCCCTGCCCGGCTGGAGCGTTTTCGGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCaa                                                                        <  1:2073286/148‑1 (MQ=255)

                                                                             tACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAg                                                                       >  1:2282881/1‑150 (MQ=255)

                                                                                    cGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgc                                                                <  2:466139/150‑1 (MQ=255)

                                                                                    cGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgc                                                                <  1:1814159/150‑1 (MQ=255)

                                                                                     gCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGa                                                                                                                           <  2:142289/90‑1 (MQ=255)

                                                                                     gCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGa                                                                                                                           >  1:142289/1‑90 (MQ=255)

                                                                                     gCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgcg                                                               >  1:1008506/1‑150 (MQ=255)

                                                                                      cccGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTAtt                                                                  >  2:908988/1‑146 (MQ=255)

                                                                                      cccGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTAtt                                                                  <  1:908988/146‑1 (MQ=255)

                                                                                        cGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCa                                                            >  2:304293/1‑150 (MQ=255)

                                                                                               gAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCaggag                                                                             >  2:527412/1‑126 (MQ=255)

                                                                                               gAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCaggag                                                                             <  1:527412/126‑1 (MQ=255)

                                                                                               gAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAActgctg                                                     >  2:117076/1‑150 (MQ=255)

                                                                                               gAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAActgctg                                                     <  1:545205/150‑1 (MQ=255)

                                                                                                  gATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGACCGC‑CCAGGCGAGCGCCGGGACGGCCCTACCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGGATTGCGCCAACTGCTGGcc                                                  >  1:1988302/1‑150 (MQ=255)

                                                                                                      ccATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTc                                              >  2:357022/1‑150 (MQ=255)

                                                                                                        aTCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGCGCCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCAt                                            >  1:1835643/1‑150 (MQ=255)

                                                                                                        aTCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCAt                                            <  1:2185205/150‑1 (MQ=255)

                                                                                                              cTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGc                                      <  2:2282881/150‑1 (MQ=255)

                                                                                                               tGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGcc                                     >  1:121259/1‑150 (MQ=255)

                                                                                                                  tCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGt                                  >  2:1800759/1‑150 (MQ=255)

                                                                                                                  tCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTAACGCCGGATGCCCAGGCATTTCGCCAACTGCTGGCCAGTCATGCCGGCCTGt                                  <  1:304293/150‑1 (MQ=255)

                                                                                                                    gACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCTCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAt                                <  1:109919/150‑1 (MQ=255)

                                                                                                                       tACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAtgtg                             >  2:1564939/1‑150 (MQ=255)

                                                                                                                         ccTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgcgc                                                              <  2:118653/115‑1 (MQ=255)

                                                                                                                          cTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATc                          <  1:168670/150‑1 (MQ=255)

                                                                                                                          cTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATc                          >  2:1800207/1‑150 (MQ=255)

                                                                                                                          cTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATc                          >  2:1644340/1‑150 (MQ=255)

                                                                                                                          cTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATc                          >  2:1619186/1‑150 (MQ=255)

                                                                                                                          cTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATc                          <  1:1280953/150‑1 (MQ=255)

                                                                                                                           ttGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGACACGCCCCGACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCg                         >  1:899378/1‑150 (MQ=255)

                                                                                                                           ttGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAActgctg                                                     <  1:809311/122‑1 (MQ=255)

                                                                                                                           ttGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGc                                                   >  2:180242/1‑124 (MQ=255)

                                                                                                                           ttGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGc                                                   <  1:180242/124‑1 (MQ=255)

                                                                                                                            tGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTTCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGa                            <  2:56903/146‑1 (MQ=255)

                                                                                                                            tGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTTCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGa                            >  1:56903/1‑146 (MQ=255)

                                                                                                                             gAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGAt                       <  1:2204764/150‑1 (MQ=255)

                                                                                                                               tcGCCTGG‑GCGCTCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCg                                                                                     >  2:2019005/2‑86 (MQ=255)

                                                                                                                               tcGCCTGG‑GCGCTCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCg                                                                                     <  1:2019005/85‑1 (MQ=255)

                                                                                                                               tcGCCTGG‑GCGCTCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAgg                                                                      >  1:1097246/2‑101 (MQ=255)

                                                                                                                               tcGCCTGG‑GCGCTCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAgg                                                                      <  2:1097246/100‑1 (MQ=255)

                                                                                                                               tcGCCTGG‑GCGCTCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATgc                     >  1:141447/2‑150 (MQ=255)

                                                                                                                               aCGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATgc                     <  1:2367570/150‑1 (MQ=255)

                                                                                                                                cGCCTGG‑GCGCTCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGAt                           >  1:2344377/1‑143 (MQ=255)

                                                                                                                                 gCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGc                                      >  2:1635750/1‑131 (MQ=255)

                                                                                                                                 gCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGc                                      <  1:1635750/131‑1 (MQ=255)

                                                                                                                                      gAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTg                                                                 <  2:2325583/99‑1 (MQ=255)

                                                                                                                                          cgtccAGGCGCTCGCCTGGGCGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCg           >  2:1274476/4‑150 (MQ=255)

                                                                                                                                              cAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCa                                                                                                               <  1:1978806/46‑1 (MQ=255)

                                                                                                                                              cAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAtg                               >  1:2189549/1‑126 (MQ=255)

                                                                                                                                              cAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAtg                               <  2:2189549/126‑1 (MQ=255)

                                                                                                                                                ggCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGTGCTg     >  1:396695/1‑150 (MQ=255)

                                                                                                                                                 gCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGTGCTgg    <  1:357022/150‑1 (MQ=255)

                                                                                                                                                   gAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGc                                      >  1:1359326/1‑114 (MQ=255)

                                                                                                                                                   gAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGc                                      <  2:1359326/114‑1 (MQ=255)

                                                                                                                                                   gAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGTGCTGGcc  >  1:1195644/1‑150 (MQ=255)

                                                                                                                                                     gcgcCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGa               <  2:1093300/135‑1 (MQ=255)

TGTCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGCGCGCGAGCCGGTGCTGGCC  >  NC_002947/5857611‑5857906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`