BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	548,806	A→C	8.2%	E551D (GAA→GAC)	fusA →	elongation factor G 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	548,806	0	A	C	8.2%	304.7 / 16.7	112	E551D (GAA→GAC)	fusA	elongation factor G 1
Reads supporting (aligned to +/- strand): ref base A (51/51); new base C (5/4); total (57/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

Only 100 of 114 total aligned reads displayed.

AAGATCACCAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAA  >  NC_002947/548657‑548955

aagaTCACGAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGaa                                                                                                                                                       <  2:1591765/150‑1 (MQ=255)

  gaTCACCAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGt                                                                                                                                                     >  1:847099/1‑150 (MQ=255)

   aTCACCAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTc                                                                                                                                                    <  1:527641/150‑1 (MQ=255)

     cACCAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGt                                                                                                                                                  >  2:1596133/1‑150 (MQ=255)

      aCCAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGtt                                                                                                                                                 >  2:1239111/1‑150 (MQ=255)

       ccAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGTGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTtg                                                                                                                                                >  2:1442690/1‑150 (MQ=255)

        cAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCGGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTtgg                                                                                                                                               >  1:809211/1‑150 (MQ=255)

        cAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTtgg                                                                                                                                               >  2:66460/1‑150 (MQ=255)

        cAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTtg                                                                                                                                                <  2:1484500/149‑1 (MQ=255)

        cAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTtg                                                                                                                                                >  1:1484500/1‑149 (MQ=255)

          aaTCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTCGATGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTtggtg                                                                                                                                             <  1:1376658/150‑1 (MQ=255)

            tCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTggtggt                                                                                                                                           <  1:1239111/150‑1 (MQ=255)

            tCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTggtggt                                                                                                                                           >  2:2017793/1‑150 (MQ=255)

            tCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCACCAACGACTTCGTtggagaa                                                                                                                                           <  2:1422697/150‑5 (MQ=255)

            tCCAACGTCGACATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTATCGGAGCCGGACGTGGACCATACCCCCATCGACGCGCAGGGGCTGGTTTTCTCCAACGAAGTCGTTggtggt                                                                                                                                           <  1:1024070/150‑1 (MQ=255)

             ccAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGgtggtg                                                                                                                                          <  1:2013535/150‑1 (MQ=255)

             ccAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGAGCTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGACTTCGTtggagaac                                                                                                                                          <  1:1355476/150‑6 (MQ=255)

               aaCGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGtgtg                                                                                                                                        >  2:2397784/1‑150 (MQ=255)

                   tCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTc                                                                                                                                    <  2:886131/150‑1 (MQ=255)

                     gagaTCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGATTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCt                                                                                                                                  >  2:1736194/1‑150 (MQ=255)

                       gaTCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAATGGTAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTaa                                                                                                                                <  2:1069625/150‑1 (MQ=255)

                       gaTCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTaa                                                                                                                                <  2:894090/150‑1 (MQ=255)

                       gaTCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTaa                                                                                                                                <  1:816423/150‑1 (MQ=255)

                        aTCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAg                                                                                                                               <  1:1574075/150‑1 (MQ=255)

                           gAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGTGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGaa                                                                                                                            <  1:473696/150‑1 (MQ=255)

                           gAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGaa                                                                                                                            <  2:1314172/150‑1 (MQ=255)

                             aGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGTGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATa                                                                                                                          <  1:1442690/150‑1 (MQ=255)

                             aGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGgtggtg                                                                                                                                          <  1:2252022/134‑1 (MQ=255)

                                  aGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATcc                                                                                                                     <  2:2247572/150‑1 (MQ=255)

                                      cgttcgtCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGc                                                                                                                 >  2:410181/1‑150 (MQ=255)

                                          cgtCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATc                                                                                                             <  1:1993870/150‑1 (MQ=255)

                                               gTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGaa                                                                                                        <  1:2364893/150‑1 (MQ=255)

                                               gTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCGTCTCCGAGTCGGACGTGGACGACAAGGGCAACATCACAGAAGCTCTCGTCTTCTCCAACGAAGTCGGTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGaa                                                                                                        <  2:639678/150‑1 (MQ=255)

                                                  gggTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAggg                                                                                                     >  2:1459851/1‑150 (MQ=255)

                                                  gggTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAggg                                                                                                     >  1:861436/1‑150 (MQ=255)

                                                   ggtggtCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGCCTACTCCAACGTAGGCGTTGGNCGAGTGATTCCTAAGGAATACATCCATGCGAGCCAAAAggtt                                                                                                    >  2:572922/1‑148 (MQ=255)

                                                    gtggtcgtggtcAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTa                                                                                                   <  1:2017793/150‑1 (MQ=255)

                                                     ttctcgtgggcAGTTCGGTCACTGCTGGGTTCGTTTCTCCGTGGCGGACTTGGACGACAAGGGCAACATGACAGAAGGTTTACTGTTCTGCTGCGAGGTCGTTGGTGGTGTGAATCCTAAGGAAAAGATCCCTGCCATCGAGAATGGTAt                                                                                                  >  2:760795/4‑150 (MQ=255)

                                                      ggtcgtggtcAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATc                                                                                                 <  1:66460/150‑1 (MQ=255)

                                                       gtcgtggtcAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCg                                                                                                >  1:1526287/1‑150 (MQ=255)

                                                       gtcgtggtcAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCg                                                                                                <  1:410181/150‑1 (MQ=255)

                                                        tcgtggtcAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGa                                                                                               >  1:452088/1‑150 (MQ=255)

                                                          gtggtcAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAag                                                                                             <  1:2404412/150‑1 (MQ=255)

                                                              tcAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCa                                                                                         >  1:1012200/1‑150 (MQ=255)

                                                              tcAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCa                                                                                         >  2:1904520/1‑150 (MQ=255)

                                                                      gTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAAt                                                                                                                           >  2:1631345/1‑108 (MQ=255)

                                                                      gTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAAt                                                                                                                           <  1:1631345/108‑1 (MQ=255)

                                                                           tgctgGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTc                                                                                                                                                    >  1:966197/1‑78 (MQ=255)

                                                                           tgctgGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGc                                                                            <  2:1012200/150‑1 (MQ=255)

                                                                             ctgGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCgt                                                                          >  1:1461051/1‑150 (MQ=255)

                                                                              tgGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCgtt                                                                         <  2:847099/150‑1 (MQ=255)

                                                                               gggTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAg                                                                                                       <  1:875518/119‑1 (MQ=255)

                                                                               gggTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAg                                                                                                       >  2:875518/1‑119 (MQ=255)

                                                                                 gTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCgttgtt                                                                      <  1:2397784/150‑1 (MQ=255)

                                                                                  ttCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTCAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGttgttg                                                                     >  2:2158531/1‑150 (MQ=255)

                                                                                   tCGCTTCTCCGAGCCGGACGTGCACGACAAGCGCAACAGCACACAAGGTCGGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGTTATCGAAGAGCAGATGAAAAACGGCGTTGTTGc                                                                    <  1:572922/150‑1 (MQ=255)

                                                                                     gCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTTGATATGAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCg                                                                  >  1:151031/1‑150 (MQ=255)

                                                                                     gCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCg                                                                  <  1:2040691/150‑1 (MQ=255)

                                                                                     gCTTCTCCGAGCCGGAAGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCg                                                                  >  2:17047/1‑150 (MQ=255)

                                                                                      cTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCgg                                                                 >  2:938150/1‑150 (MQ=255)

                                                                                       ttCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGt                                                                >  1:757539/1‑150 (MQ=255)

                                                                                        tctcCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGtt                                                               >  1:382135/1‑150 (MQ=255)

                                                                                              ggCCGGACGTGTAAGACAAGGGCAACATCACAGAAGGTCGGCTGTGTTCCAACCAAGTCGTTGGTGGAGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGCCTATCGAAGAGCAGATGAAAAACGTCGTTGTTGCCGGTTATCctc                                                         <  2:317986/149‑1 (MQ=255)

                                                                                                 cGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGa                                                      >  2:90660/1‑150 (MQ=255)

                                                                                                  ggACGTGGACGACAAGGCCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGAt                                                     >  1:573053/1‑150 (MQ=255)

                                                                                                      gTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGc                                                 <  1:1736194/150‑1 (MQ=255)

                                                                                                        ggACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCt                                               >  2:368300/1‑150 (MQ=255)

                                                                                                         gacgacAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGc                                                 >  2:41594/1‑147 (MQ=255)

                                                                                                           cgacAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGaa                                            <  1:1236538/150‑1 (MQ=255)

                                                                                                           cgacAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGaa                                            <  1:90660/150‑1 (MQ=255)

                                                                                                           cgacAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGaa                                            <  1:368300/150‑1 (MQ=255)

                                                                                                               aaGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCGTCTGATCGGCCTGAAGGcc                                        >  1:1105708/1‑150 (MQ=255)

                                                                                                                 gggCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCcgc                                      >  1:943866/1‑150 (MQ=255)

                                                                                                                  ggCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTaa                                                                                                                                >  2:1303289/1‑59 (MQ=255)

                                                                                                                  ggCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTaa                                                                                                                                <  1:1303289/59‑1 (MQ=255)

                                                                                                                   gCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGc                                                 <  1:113517/137‑1 (MQ=255)

                                                                                                                   gCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGc                                                 >  2:113517/1‑137 (MQ=255)

                                                                                                                   gCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCgg                                    <  1:1459851/150‑1 (MQ=255)

                                                                                                                   gCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCgg                                    <  1:1596133/150‑1 (MQ=255)

                                                                                                                        aTCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGGGGTAttg                               >  1:1110398/1‑149 (MQ=255)

                                                                                                                         tCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAg                                                                                        >  1:626236/1‑92 (MQ=255)

                                                                                                                          cacaGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGa                             <  2:151031/150‑1 (MQ=255)

                                                                                                                            caGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACg                           >  2:707813/1‑150 (MQ=255)

                                                                                                                            caGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCATTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACg                           <  2:861436/150‑1 (MQ=255)

                                                                                                                                        tgtTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAg                                                                                                       >  2:2117493/1‑62 (MQ=255)

                                                                                                                                        acaccaccAACGACTTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGaa                                            <  2:1631275/115‑1 (MQ=255)

                                                                                                                                        acaccaccAACGACTTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGaa                                            >  1:1631275/7‑121 (MQ=255)

                                                                                                                                        acaccaccAACGACTTCGTTGGAGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGGCGCGGTATTCGACGGTTCGTacgacg               >  2:741820/7‑150 (MQ=255)

                                                                                                                                        acaccaccAACGACTTCGTTGGAGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCacg               >  2:743090/7‑150 (MQ=255)

                                                                                                                                         caccaccAACGACTTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCacga              >  2:699275/6‑150 (MQ=255)

                                                                                                                                          accaccAACGACTTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTAtt                                >  2:1426865/5‑131 (MQ=255)

                                                                                                                                          accaccAACGACTTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTAtt                                <  1:1426865/127‑1 (MQ=255)

                                                                                                                                             tcCAAGGAAGTCATTGGTGTTTTGATTCCTAATTAATATATCTCTTCAATCCAGAATCGTATCGAAGAGCAGATGAAAAACTGCGTTGTTGCCGGTTATCTTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTc          <  2:452088/150‑1 (MQ=255)

                                                                                                                                             tcCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTc          >  1:1780627/1‑150 (MQ=255)

                                                                                                                                                aaCGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATc                                                    <  1:766692/105‑1 (MQ=255)

                                                                                                                                                aaCGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTAtt                                >  1:1845936/1‑125 (MQ=255)

                                                                                                                                                aaCGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTAtt                                <  2:1845936/125‑1 (MQ=255)

                                                                                                                                                aaCGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGAc       >  1:2080734/1‑150 (MQ=255)

                                                                                                                                                aaCGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGAc       >  2:2013845/1‑150 (MQ=255)

                                                                                                                                                     aGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCaa  <  2:1780627/150‑1 (MQ=255)

AAGATCACCAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTATCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAA  >  NC_002947/548657‑548955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`