Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,886,820 C→G 5.6% W84C (TGG→TGC)  PP_5161 ← lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,886,8200CG 5.6% 284.4 / 6.3 89W84C (TGG→TGC) PP_5161lysophospholipase
Reads supporting (aligned to +/- strand):  ref base C (43/41);  new base G (3/2);  total (46/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

TGGACAGGCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGTGCGCAG  >  NC_002947/5886678‑5886969
                                                                                                                                              |                                                                                                                                                      
tGGACAGGCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCAtgcat                                                                                                                                                 >  2:1177866/1‑148 (MQ=255)
tGGACAGGCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCATGGCTGCCCGAGCAGCCaagcgc                                                                                                                                                 <  2:1621045/150‑6 (MQ=255)
             gCCGTGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTc                                                                                                                                    >  2:2154979/1‑150 (MQ=255)
              ccGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCg                                                                                                                                   >  1:2312130/1‑150 (MQ=255)
                gggCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGaa                                                                                                                                 <  2:1356651/150‑1 (MQ=255)
                  gCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAAcc                                                                                                                               <  1:14612/150‑1 (MQ=255)
                    aGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAAcccc                                                                                                                             >  2:853326/1‑150 (MQ=255)
                       tCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCt                                                                                                                          >  2:924291/1‑150 (MQ=255)
                             cTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGa                                                                                                                    <  2:2312130/150‑1 (MQ=255)
                             cTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGa                                                                                                                    <  1:924291/150‑1 (MQ=255)
                               gATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGaaa                                                                                                                  <  2:1204808/150‑1 (MQ=255)
                               gATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGaaa                                                                                                                  <  1:1320656/150‑1 (MQ=255)
                                 tCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGGTTGGCTACTCGGGCAGCCATGGCTTCCCGACCAGGTCGGACCCCGCTGCCTGAAAGc                                                                                                                >  2:266955/1‑150 (MQ=255)
                                   aCGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTa                                                                                                              >  1:1651969/1‑150 (MQ=255)
                                     ggggAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTAcc                                                                                                            >  1:2403065/4‑150 (MQ=255)
                                     ggCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCt                                                                                                                      <  1:1803735/140‑1 (MQ=255)
                                     ggCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCt                                                                                                                      >  2:1803735/1‑140 (MQ=255)
                                         aaaCCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACccagc                                                                                                        <  1:2154979/150‑1 (MQ=255)
                                             ccTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGGTCGGGCAGGCATGGCTGCCCGagca                                                                                                                                        >  2:1885682/1‑111 (MQ=39)
                                             ccTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGGTCGGGCAGGCATGGCTGCCCGAGCa                                                                                                                                        <  1:1885682/114‑1 (MQ=39)
                                             ccTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCcagccagc                                                                                                    >  1:1858002/1‑150 (MQ=255)
                                             ccTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCcagccagc                                                                                                    >  2:176339/1‑150 (MQ=255)
                                               tGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGcc                                                                                                                                                       <  2:1741139/97‑1 (MQ=255)
                                               tGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTAGGGAAGcc                                                                                                                                                       >  1:1741139/1‑97 (MQ=255)
                                                gCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCtgt                                                                                                 <  1:722679/150‑1 (MQ=255)
                                                    gAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCtgt                                                                                                 >  1:1864955/1‑146 (MQ=255)
                                                    gAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCtgt                                                                                                 <  2:1864955/146‑1 (MQ=255)
                                                          ccACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGGTCGGGCAGGCATGGCTGCCCGagca                                                                                                                                        >  1:451292/1‑98 (MQ=38)
                                                          ccACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGGTCGGGCAGGCATGGCTGCCCGAGCa                                                                                                                                        <  2:451292/101‑1 (MQ=38)
                                                          ccACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAg                                                                                      <  2:1545435/150‑1 (MQ=255)
                                                          ccACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCCCGAGCAGCCaa                                                                                                                                                     <  1:1010505/88‑2 (MQ=255)
                                                          ccACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCCCGAGCAGCCaa                                                                                                                                                     >  2:1010505/1‑87 (MQ=255)
                                                            aCTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGt                                                                                    >  1:260509/1‑150 (MQ=255)
                                                            aCTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGt                                                                                    >  1:515480/1‑150 (MQ=255)
                                                             cTCGATCACATGTCGGTAGAGGCCGATGTGGTCGTAATAGCCGTGCAGCAGGAACAGGGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCTGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CTGGtt                                                                                   >  1:337621/1‑150 (MQ=255)
                                                                gATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGc                                                                                                                                                        <  1:787920/79‑1 (MQ=255)
                                                                gATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGc                                                                                                                                                        >  2:787920/1‑79 (MQ=255)
                                                                gATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAg                                                                                <  2:1548143/150‑1 (MQ=255)
                                                                    acaTGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCa                                                                            <  1:1996210/150‑1 (MQ=255)
                                                                      aTGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGaaa                                                                                                                  <  2:1981795/111‑1 (MQ=255)
                                                                      aTGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGaaa                                                                                                                  >  1:1981795/1‑111 (MQ=255)
                                                                      aTGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAg                                                                                <  2:2115021/144‑1 (MQ=255)
                                                                      aTGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAg                                                                                >  1:2115021/1‑144 (MQ=255)
                                                                      aTGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTAGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATa                                                                          >  1:667208/1‑150 (MQ=255)
                                                                         tCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGaa                                                                       <  2:745914/150‑1 (MQ=255)
                                                                           ggTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTc                                                                                                                                    <  1:1383042/88‑1 (MQ=255)
                                                                           ggTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTc                                                                                                                                    >  2:1383042/1‑88 (MQ=255)
                                                                            gTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAgcg                                                                    >  2:1104573/1‑150 (MQ=255)
                                                                            gTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAgcg                                                                    >  1:2374473/1‑150 (MQ=255)
                                                                               gagGCCCATGTAGTCATAATAGCCGTACAGCAGGAACAGCGTCGCGCTACGCGGCTCAGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTCCATGAANGNTNCAAAACCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAAAAGCGCTg                                                                 <  1:1507663/150‑1 (MQ=255)
                                                                                  gCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAgg                                                                               <  2:2310828/133‑1 (MQ=255)
                                                                                  gCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAgg                                                                               >  1:2310828/1‑133 (MQ=255)
                                                                                  gCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTa                                                              >  1:1156781/1‑150 (MQ=255)
                                                                                    ccATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAgcgcaggacta                                                            >  2:1435423/1‑143 (MQ=255)
                                                                                           gTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAg                                                                                >  1:1215225/1‑123 (MQ=255)
                                                                                           gTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAg                                                                                <  2:1215225/123‑1 (MQ=255)
                                                                                           gTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTgcgc                                                     >  2:473607/1‑150 (MQ=255)
                                                                                            tCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAg                                                                                      >  1:883544/1‑116 (MQ=255)
                                                                                            tCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAg                                                                                      <  2:883544/116‑1 (MQ=255)
                                                                                            tCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCt                                                    >  1:79197/1‑150 (MQ=255)
                                                                                              gTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTtg                                                  >  2:1573025/1‑150 (MQ=255)
                                                                                                aaTAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTtgtg                                                >  2:1709042/1‑150 (MQ=255)
                                                                                                   aGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCg                                             >  2:2112125/1‑150 (MQ=255)
                                                                                                      cGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACa                                          <  2:2198696/150‑1 (MQ=255)
                                                                                                      cGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACa                                          <  1:473607/150‑1 (MQ=255)
                                                                                                       gTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCtt                                                   >  1:1380722/1‑140 (MQ=255)
                                                                                                       gTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCtt                                                   <  2:1380722/140‑1 (MQ=255)
                                                                                                            gcagGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGtggct                                    >  2:1483670/1‑150 (MQ=255)
                                                                                                            gcagGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGtggct                                    >  1:407522/1‑150 (MQ=255)
                                                                                                             cagGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCtt                                                   <  2:1024987/134‑1 (MQ=255)
                                                                                                             cagGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCtt                                                   >  1:1024987/1‑134 (MQ=255)
                                                                                                              agGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGtggctgg                                  <  1:2112125/150‑1 (MQ=255)
                                                                                                                gAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCt                                                                  >  1:869426/1‑116 (MQ=255)
                                                                                                                gAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCt                                                                  <  2:869426/116‑1 (MQ=255)
                                                                                                                  aCAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAg                              >  2:1396396/1‑150 (MQ=255)
                                                                                                                    aGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAgtg                            <  2:1156781/150‑1 (MQ=255)
                                                                                                                    aGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAgtg                            <  2:515480/150‑1 (MQ=255)
                                                                                                                    aGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAgtc                            <  2:1651969/150‑2 (MQ=255)
                                                                                                                        tCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGa                        >  2:663293/1‑150 (MQ=255)
                                                                                                                           gttcgacctggtCGGGCAGGCATGGCTGCCCGAGCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACg                     >  1:2106338/8‑150 (MQ=39)
                                                                                                                              ttGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGgcg                  >  1:2312073/1‑150 (MQ=255)
                                                                                                                                    gctCGGGCAGCCATGCCTGCCCGACCAGGTCGCACCCCGCTGAGTGAAAGCTATCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGc            <  1:1966572/150‑1 (MQ=255)
                                                                                                                                      tCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTgg                                                                >  2:393983/1‑96 (MQ=255)
                                                                                                                                      tCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTgg                                                                <  1:393983/96‑1 (MQ=255)
                                                                                                                                      tCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCgg          <  1:1573025/150‑1 (MQ=255)
                                                                                                                                      tCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCgg          <  1:1483670/150‑1 (MQ=255)
                                                                                                                                      tCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCgg          <  1:1104573/150‑1 (MQ=255)
                                                                                                                                       cGGGCCGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGCACACTACCCAGCGTG‑TGGCTGGCGGTCATGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCggg         <  1:285384/150‑1 (MQ=255)
                                                                                                                                       cGGGCAGCCATGCCTGCCCGACAAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCggg         <  1:1177866/150‑1 (MQ=255)
                                                                                                                                        gggCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGt        <  2:407522/150‑1 (MQ=255)
                                                                                                                                        gggCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGt        <  1:852674/150‑1 (MQ=255)
                                                                                                                                          gCAGCCATGCCTGCCCGAGCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGTgc      >  2:1362003/1‑150 (MQ=255)
                                                                                                                                          gCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGTgg      >  1:1609749/1‑149 (MQ=255)
                                                                                                                                              ccATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGTGCGCAg  <  1:266955/150‑1 (MQ=255)
                                                                                                                                              ccATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGTGCGCAg  <  2:2403065/150‑1 (MQ=255)
                                                                                                                                              ccATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGTGCGCAg  <  2:1858002/150‑1 (MQ=255)
                                                                                                                                              ccATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGTGCGCAg  >  1:1370204/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                      
TGGACAGGCCGTGGCCGGGCAGGTCACAGCTGATCACGGCGAAACCCTGCTTGAGTGCCCACTCGATCACATGTCGGTAGAGGCCCATGTGGTCGTAATAGCCGTGCAGCAGGAACAGCGTCGCGCTTGGCTGCTCGGGCAGCCATGCCTGCCCGACCAGGTCGAACCCCGCTGCCTGAAAGCTACCCAGCCAGCTGTGTGCCGG‑CAGGTTCAGGCCATAGAAGCGCTGGTAGTCCTGCGCTTGTGCCGACAGTGGCTGGCGAGTGGTGAACGGCGCCAGGCGGGTGCGCAG  >  NC_002947/5886678‑5886969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑