BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	548,807	G→T	8.0%	V552F (GTC→TTC)	fusA →	elongation factor G 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	548,807	0	G	T	8.0%	346.9 / 13.4	113	V552F (GTC→TTC)	fusA	elongation factor G 1
Reads supporting (aligned to +/- strand): ref base G (52/52); new base T (5/4); total (57/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 115 total aligned reads displayed.

GATCACCAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAAC  >  NC_002947/548659‑548956

gaTCACCAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGt                                                                                                                                                       >  1:847099/1‑150 (MQ=255)

 aTCACCAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTc                                                                                                                                                      <  1:527641/150‑1 (MQ=255)

    aCCAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGtt                                                                                                                                                   >  2:1239111/1‑150 (MQ=255)

     ccAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGTGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTtg                                                                                                                                                  >  2:1442690/1‑150 (MQ=255)

      cAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCGGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTtgg                                                                                                                                                 >  1:809211/1‑150 (MQ=255)

      cAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTtgg                                                                                                                                                 >  2:66460/1‑150 (MQ=255)

      cAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTtg                                                                                                                                                  <  2:1484500/149‑1 (MQ=255)

        aaTCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTCGATGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTtggtg                                                                                                                                               <  1:1376658/150‑1 (MQ=255)

          tCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTggtggt                                                                                                                                             >  2:2017793/1‑150 (MQ=255)

          tCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTggtggt                                                                                                                                             <  1:1239111/150‑1 (MQ=255)

          tCCAACGTCGACATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTATCGGAGCCGGACGTGGACCATACCCCCATCGACGCGCAGGGGCTGGTTTTCTCCAACGAAGTCGTTggtggt                                                                                                                                             <  1:1024070/150‑1 (MQ=255)

           ccAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGgtggtg                                                                                                                                            <  1:2013535/150‑1 (MQ=255)

           ccAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGAGCTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGACTTCGTtggagaac                                                                                                                                            <  1:1355476/150‑6 (MQ=255)

                 tCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTc                                                                                                                                      <  2:886131/150‑1 (MQ=255)

                   gagaTCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGCTGCTGGTGTGATTCCt                                                                                                                                    >  2:178393/1‑150 (MQ=255)

                   gagaTCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGATTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCt                                                                                                                                    >  2:1736194/1‑150 (MQ=255)

                    agaTCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTa                                                                                                                                   >  1:894090/1‑150 (MQ=255)

                     gaTCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAATGGTAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTaa                                                                                                                                  <  2:1069625/150‑1 (MQ=255)

                     gaTCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTaa                                                                                                                                  <  2:894090/150‑1 (MQ=255)

                      aTCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAg                                                                                                                                 <  1:1574075/150‑1 (MQ=255)

                         gAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGTGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGaa                                                                                                                              <  1:473696/150‑1 (MQ=255)

                         gAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGaa                                                                                                                              <  2:1314172/150‑1 (MQ=255)

                           aGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGTGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATa                                                                                                                            <  1:1442690/150‑1 (MQ=255)

                           aGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGgtggtg                                                                                                                                            <  1:2252022/134‑1 (MQ=255)

                           aGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGgtggtg                                                                                                                                            >  2:2252022/1‑134 (MQ=255)

                                aGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATcc                                                                                                                       <  2:2247572/150‑1 (MQ=255)

                                    cgttcgtCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGc                                                                                                                   >  2:410181/1‑150 (MQ=255)

                                        cgtCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATc                                                                                                               <  1:1993870/150‑1 (MQ=255)

                                             gTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGaa                                                                                                          <  1:2364893/150‑1 (MQ=255)

                                               cGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCGTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAgg                                                                                                        <  1:1774892/150‑1 (MQ=255)

                                                gggTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAggg                                                                                                       >  1:861436/1‑150 (MQ=255)

                                                gggTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAggg                                                                                                       >  2:1459851/1‑150 (MQ=255)

                                                 ggtggtCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGCCTACTCCAACGTAGGCGTTGGNCGAGTGATTCCTAAGGAATACATCCATGCGAGCCAAAAggtt                                                                                                      >  2:572922/1‑148 (MQ=255)

                                                   ttctcgtgggcAGTTCGGTCACTGCTGGGTTCGTTTCTCCGTGGCGGACTTGGACGACAAGGGCAACATGACAGAAGGTTTACTGTTCTGCTGCGAGGTCGTTGGTGGTGTGAATCCTAAGGAAAAGATCCCTGCCATCGAGAATGGTA‑t                                                                                                   >  2:760795/4‑150 (MQ=255)

                                                    ggtcgtggtcAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑Tc                                                                                                  <  1:66460/150‑1 (MQ=255)

                                                     gtcgtggtcAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCg                                                                                                 >  1:1526287/1‑150 (MQ=255)

                                                     gtcgtggtcAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCg                                                                                                 <  1:410181/150‑1 (MQ=255)

                                                      tcgtggtcAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGa                                                                                                >  1:452088/1‑150 (MQ=255)

                                                        gtggtcAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAag                                                                                              <  1:2404412/150‑1 (MQ=255)

                                                            tcAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCa                                                                                          >  1:1012200/1‑150 (MQ=255)

                                                                   ggTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGaaa                                                                                   >  1:754260/1‑150 (MQ=255)

                                                                    gTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAAt                                                                                                                             >  2:1631345/1‑108 (MQ=255)

                                                                     tCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGaaaaa                                                                                 <  2:1526287/150‑1 (MQ=255)

                                                                         tgctgGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTc                                                                                                                                                      >  1:966197/1‑78 (MQ=255)

                                                                         tgctgGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGaa                                                                                                          <  1:1306107/122‑1 (MQ=255)

                                                                         tgctgGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGaa                                                                                                          >  2:1306107/1‑122 (MQ=255)

                                                                           ctgGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCgt                                                                           >  1:1461051/1‑150 (MQ=255)

                                                                             gggTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAg                                                                                                         >  2:875518/1‑119 (MQ=255)

                                                                             gggTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAg                                                                                                         <  1:875518/119‑1 (MQ=255)

                                                                               gTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCgttgtt                                                                       <  1:2397784/150‑1 (MQ=255)

                                                                                ttCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTCAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGttgttg                                                                      >  2:2158531/1‑150 (MQ=255)

                                                                                   gCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTTGATATGAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCg                                                                   >  1:151031/1‑150 (MQ=255)

                                                                                   gCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCg                                                                   <  1:2040691/150‑1 (MQ=255)

                                                                                   gCTTCTCCGAGCCGGAAGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCg                                                                   >  2:17047/1‑150 (MQ=255)

                                                                                    cTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCgg                                                                  >  2:938150/1‑150 (MQ=255)

                                                                                     ttCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGt                                                                 >  1:757539/1‑150 (MQ=255)

                                                                                      tctcCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGtt                                                                >  1:382135/1‑150 (MQ=255)

                                                                                            ggCCGGACGTGTAAGACAAGGGCAACATCACAGAAGGTCGGCTGTGTTCCAACCAAGTCGTTGGTGGAGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGCCTA‑TCGAAGAGCAGATGAAAAACGTCGTTGTTGCCGGTTATCctc                                                          <  2:317986/149‑1 (MQ=255)

                                                                                               cGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGa                                                       >  2:90660/1‑150 (MQ=255)

                                                                                                ggACGTGGACGACAAGGCCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGAt                                                      >  1:573053/1‑150 (MQ=255)

                                                                                                    gTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGc                                                  <  1:1736194/150‑1 (MQ=255)

                                                                                                      ggACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCt                                                >  2:368300/1‑150 (MQ=255)

                                                                                                      ggACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAAGACATCCGTCAGAT‑CGGAAGAGCACACGTCTGACATATGGAAAACGGCGTTGTTGCCGGTTATCGGCTGAGCAGCCt                                                >  1:1488175/1‑150 (MQ=255)

                                                                                                       gacgacAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGc                                                  <  1:41594/147‑1 (MQ=255)

                                                                                                       gacgacAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGc                                                  >  2:41594/1‑147 (MQ=255)

                                                                                                         cgacAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGaa                                             <  1:1236538/150‑1 (MQ=255)

                                                                                                         cgacAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGaa                                             <  1:90660/150‑1 (MQ=255)

                                                                                                             aaGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCGTCTGATCGGCCTGAAGGcc                                         >  1:1105708/1‑150 (MQ=255)

                                                                                                               gggCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCcgc                                       >  1:943866/1‑150 (MQ=255)

                                                                                                                ggCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTaa                                                                                                                                  >  2:1303289/1‑59 (MQ=255)

                                                                                                                 gCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGc                                                  >  2:113517/1‑137 (MQ=255)

                                                                                                                 gCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGc                                                  <  1:113517/137‑1 (MQ=255)

                                                                                                                 gCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCgg                                     <  1:1596133/150‑1 (MQ=255)

                                                                                                                 gCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCgg                                     <  1:1459851/150‑1 (MQ=255)

                                                                                                                      aTCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGGGGTAttg                                >  1:1110398/1‑149 (MQ=255)

                                                                                                                       tCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAg                                                                                         <  2:626236/92‑1 (MQ=255)

                                                                                                                       tCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAg                                                                                         >  1:626236/1‑92 (MQ=255)

                                                                                                                        cacaGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGa                              <  2:151031/150‑1 (MQ=255)

                                                                                                                          caGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACg                            >  2:707813/1‑150 (MQ=255)

                                                                                                                          caGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCATTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACg                            <  2:861436/150‑1 (MQ=255)

                                                                                                                                      tgtTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAg                                                                                                         <  1:2117493/62‑1 (MQ=255)

                                                                                                                                      tgtTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAg                                                                                                         >  2:2117493/1‑62 (MQ=255)

                                                                                                                                      acaccaccAACGACTTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGaa                                             >  1:1631275/7‑121 (MQ=255)

                                                                                                                                      acaccaccAACGACTTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGaa                                             <  2:1631275/115‑1 (MQ=255)

                                                                                                                                      acaccaccAACGACTTCGTTGGAGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGGCGCGGTATTCGACGGTTCGTacgacg                >  2:741820/7‑150 (MQ=255)

                                                                                                                                      acaccaccAACGACTTCGTTGGAGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCacg                >  2:743090/7‑150 (MQ=255)

                                                                                                                                       caccaccAACGACTTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCacga               >  2:699275/6‑150 (MQ=255)

                                                                                                                                        accaccAACGACTTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTAtt                                 <  1:1426865/127‑1 (MQ=255)

                                                                                                                                        accaccAACGACTTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTAtt                                 >  2:1426865/5‑131 (MQ=255)

                                                                                                                                           tcCAAGGAAGTCATTGGTGTTTTGATTCCTAATTAATATATCTCTTCAATCCAGAATCGTA‑TCGAAGAGCAGATGAAAAACTGCGTTGTTGCCGGTTATCTTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTc           <  2:452088/150‑1 (MQ=255)

                                                                                                                                           tcCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTc           >  1:1780627/1‑150 (MQ=255)

                                                                                                                                              aaCGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATc                                                     >  2:766692/1‑105 (MQ=255)

                                                                                                                                              aaCGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATc                                                     <  1:766692/105‑1 (MQ=255)

                                                                                                                                              aaCGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTAtt                                 <  2:1845936/125‑1 (MQ=255)

                                                                                                                                              aaCGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTAtt                                 >  1:1845936/1‑125 (MQ=255)

                                                                                                                                              aaCGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGAc        >  1:2080734/1‑150 (MQ=255)

                                                                                                                                              aaCGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGAc        >  2:2013845/1‑150 (MQ=255)

                                                                                                                                                   aGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCaa   <  2:1780627/150‑1 (MQ=255)

                                                                                                                                                    gTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAAc  <  1:1904520/150‑1 (MQ=255)

                                                                                                                                                    gTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAAc  <  2:1461051/150‑1 (MQ=255)

GATCACCAAATCCAACGTCGAGATCGAAGGCAAGTTCGTTCGTCAGTCGGGTGGTCGTGGTCAGTTCGGTCACTGCTGGGTTCGCTTCTCCGAGCCGGACGTGGACGACAAGGGCAACATCACAGAAGGTCTGGTGTTCTCCAACGAAGTCGTTGGTGGTGTGATTCCTAAGGAATACATCCCTGCGATCCAGAAGGGTA‑TCGAAGAGCAGATGAAAAACGGCGTTGTTGCCGGTTATCCTCTGATCGGCCTGAAGGCCGCGGTATTCGACGGTTCGTACCACGACGTCGACTCCAAC  >  NC_002947/548659‑548956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`