Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,950,028 G→C 5.0% G230A (GGC→GCC)  ppx → exopolyphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,950,0280GC 5.0% 358.0 / 5.7 111G230A (GGC→GCC) ppxexopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base G (53/51);  new base C (3/3);  total (56/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

AAGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCGTCAAACCAGACCGCCGCGCTATCTTCCCGGCGGGCTTGGCGATTCTCGAA  >  NC_002947/5949880‑5950176
                                                                                                                                                     |                                                                                                                                                    
aaGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCcggc                                                                                                                                                     >  1:1827697/1‑150 (MQ=255)
aaGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCCGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCcggc                                                                                                                                                     >  1:770480/1‑150 (MQ=255)
 aGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCcggcg                                                                                                                                                    <  2:2346139/150‑1 (MQ=255)
 aGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCcggcg                                                                                                                                                    <  1:1653862/150‑1 (MQ=255)
   aTCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCggcggc                                                                                                                                                  >  2:902263/1‑150 (MQ=255)
    tCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGcc                                                                                                                                                 >  1:800862/1‑150 (MQ=255)
    tCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGcc                                                                                                                                                 <  2:2133182/150‑1 (MQ=255)
      aCCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCcggcg                                                                                                                                                    <  1:724432/145‑1 (MQ=255)
      aCCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCcggcg                                                                                                                                                    >  2:724432/1‑145 (MQ=255)
      aCCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTg                                                                                                                                               <  2:1863599/150‑1 (MQ=255)
      aCCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTg                                                                                                                                               >  1:1571238/1‑150 (MQ=255)
       ccccGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTgg                                                                                                                                              >  1:89528/1‑150 (MQ=255)
       ccccGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTgg                                                                                                                                              <  1:1887488/150‑1 (MQ=255)
       ccccGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTgg                                                                                                                                              <  2:1827697/150‑1 (MQ=255)
        cccGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTggg                                                                                                                                             >  1:714804/1‑150 (MQ=255)
         ccGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGCCGGCCttgat                                                                                                                                            <  1:1411240/150‑5 (MQ=255)
            gCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAAt                                                                                                                                         >  1:12987/1‑150 (MQ=255)
            gCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAAt                                                                                                                                         <  2:779208/150‑1 (MQ=255)
             cccGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGCATTCCGATGAGGCTATCTG‑TGGATTCGTCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATg                                                                                                                                        <  1:902263/150‑1 (MQ=255)
              ccGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATgg                                                                                                                                       >  2:1165965/1‑150 (MQ=255)
               cGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGc                                                                                                                                      <  2:12987/150‑1 (MQ=255)
                 cTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCTCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGa                                                                                                                                    >  1:2261306/1‑150 (MQ=255)
                 cTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGa                                                                                                                                    <  1:77537/150‑1 (MQ=255)
                  tATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAg                                                                                                                                   <  2:1722765/150‑1 (MQ=255)
                  tATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAg                                                                                                                                   <  1:2321898/150‑1 (MQ=255)
                       ccAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCgttgacct                                                                                                                              >  1:488808/1‑143 (MQ=255)
                           gCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGcc                                                                                                                          <  1:70994/150‑1 (MQ=255)
                             cTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGCCGGCCttgat                                                                                                                                            <  1:2324128/130‑5 (MQ=255)
                             cTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGCCGGCCttgat                                                                                                                                            >  2:2324128/1‑126 (MQ=255)
                              tACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCAG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGaa                                                                                                                       >  2:1529110/1‑150 (MQ=255)
                               acacTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAg                                                                                                                      >  2:2137469/1‑150 (MQ=255)
                                 acTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTc                                                                                                                                >  1:196592/1‑138 (MQ=255)
                                 acTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTc                                                                                                                                <  2:196592/138‑1 (MQ=255)
                                 acTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAggc                                                                                                                    <  1:1113360/150‑1 (MQ=255)
                                    gCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAggcctg                                                                                                                 <  1:484655/150‑1 (MQ=255)
                                     ccGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGt                                                                                                                                 >  1:982/1‑133 (MQ=255)
                                     ccGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAggcctgg                                                                                                                <  2:1571238/150‑1 (MQ=255)
                                      cGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAggcctggc                                                                                                               <  2:1936901/150‑1 (MQ=255)
                                         aCGCCGGGACCTGAGGAGCATCAAAAATGCCCGCCACCCCCTGACCTGGGATG‑GGCTTTCGGCCTCATCCGCAACCATTCGCGCCATCGCCGCGGCAACAAAGGCCGG‑GGGCTGGGCAATGGCGAGGTCAACGCCGAAGGcctggcctgg                                                                                                           <  2:2051585/150‑1 (MQ=255)
                                          cGCCTGGAACTGATGAGCATCGAACAGGCCCTGCACCGCCTGACCTGGGATGGGGCTATTGG‑CTCATCCGGCACCCTCCTAACCATCGGCATCGCGGTCATGTCCACCTTCCTTGCCGATGGCGAGGTCAACGCCGAAGGcctggcctgg                                                                                                           <  1:1854373/150‑1 (MQ=255)
                                           gCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTggg                                                                                                          >  1:1714208/1‑150 (MQ=255)
                                                gAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCaaa                                                                                                     <  2:1714208/150‑1 (MQ=255)
                                                 aaCTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAAc                                                                                                    <  1:1165965/150‑1 (MQ=255)
                                                 aaCTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGATCAACGCCGAAGGCCTGGCCTGGGTCAAAc                                                                                                    <  2:1517733/150‑1 (MQ=255)
                                                     gatgaGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCaa                                                                                                <  2:714804/150‑1 (MQ=255)
                                                        gaGCATCGAAAATGTCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAAGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCt                                                                                             >  1:633179/1‑150 (MQ=255)
                                                           cATCGAAAATGCCCTGCACCGCCTGGGTTGTAAGGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGtt                                                                                          >  1:1871296/1‑150 (MQ=255)
                                                           cATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGtt                                                                                          >  2:128701/1‑150 (MQ=255)
                                                            aTCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTc                                                                                         >  2:1372981/1‑150 (MQ=255)
                                                             tCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCa                                                                                        >  1:1407243/1‑150 (MQ=255)
                                                             tCGAAAATGCCCTGCACCGCCTGACCTAGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCa                                                                                        >  1:206046/1‑150 (MQ=255)
                                                                aaaaTGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGc                                                                                     <  1:2137469/150‑1 (MQ=255)
                                                                        cTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGaa                                                                             <  2:800862/150‑1 (MQ=255)
                                                                         tGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGTCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAg                                                                            <  1:2291095/150‑1 (MQ=255)
                                                                         tGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAg                                                                            >  1:786144/1‑150 (MQ=255)
                                                                            aCCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCg                                                                         >  1:53681/1‑150 (MQ=255)
                                                                                ccTGACCTGGGATGAGGCTATCGG‑CTCATCCGTCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCaaa                                                                                                     <  1:779995/118‑1 (MQ=255)
                                                                                ccTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCaaa                                                                                                     >  2:779995/1‑118 (MQ=255)
                                                                                    aCCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCCAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATc                                                                 <  1:1747107/150‑1 (MQ=255)
                                                                                      cTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGa                                                               >  1:664696/1‑150 (MQ=255)
                                                                                       tGGGATGAGGCTATCGG‑CTCATCCGGCACCATTAGCGCCATCGGCGCGGCGAGCAAGGCGGGCGGCCTGGGCAATGGTGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGAc                                                              >  1:1258902/1‑150 (MQ=255)
                                                                                        gggATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACt                                                             >  2:2101912/1‑150 (MQ=255)
                                                                                        gggATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACt                                                             <  1:1900096/150‑1 (MQ=255)
                                                                                          gATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTc                                                           <  2:53681/150‑1 (MQ=255)
                                                                                             gAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGAc                                                        <  2:471161/150‑1 (MQ=255)
                                                                                                gCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGa                                                                                                                                    >  2:285976/1‑71 (MQ=255)
                                                                                                gCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTc                                                                          <  1:2094093/129‑1 (MQ=255)
                                                                                                gCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTc                                                                          >  2:2094093/1‑129 (MQ=255)
                                                                                                 cTATCAG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCg                                                    <  1:1529110/150‑1 (MQ=255)
                                                                                                  tATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCCAACGCCAGCTGTTCAAGCTGGGCGACGTCGACAAGATCGATTTCGACGGCGt                                                   <  2:149061/150‑1 (MQ=255)
                                                                                                   aTCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCGTc                                                  <  1:2076074/150‑1 (MQ=255)
                                                                                                    tCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGGGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCGTCa                                                 >  2:497333/1‑150 (MQ=255)
                                                                                                    tCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAg                                                                                               <  1:1317515/104‑1 (MQ=255)
                                                                                                    tCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAg                                                                                               >  2:1317515/1‑104 (MQ=255)
                                                                                                          tCATCCGGCACCATTCGCGCCGTCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCGTCAAACCa                                            >  2:1314443/1‑150 (MQ=255)
                                                                                                          tCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCGTCAAACCa                                            >  1:1128050/1‑150 (MQ=255)
                                                                                                            aTCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGAt                                                                  <  2:715994/126‑1 (MQ=255)
                                                                                                            aTCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGAt                                                                  >  1:715994/1‑126 (MQ=255)
                                                                                                            aTCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCGTCAAACCAGa                                          <  2:664696/150‑1 (MQ=255)
                                                                                                             tCCGGCACCATTCGCGCCATCGGCGCGACCATCAAGGCCGGCGGCCTGGGCAATGCCGAGGTCAACGCCAAAGGCCTGGCCTGGGTCAAACGCCAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCGTCAAACCAGAc                                         >  2:832871/1‑150 (MQ=255)
                                                                                                               cGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGc                                                                                                  <  1:1332896/91‑1 (MQ=255)
                                                                                                                ggCACCATTCGCGCCATCGGCGCGGACATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTCGGCCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCGTCAAACCAGAccgc                                      >  2:293388/1‑150 (MQ=255)
                                                                                                                     cATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCgccca                                                      <  1:1326249/129‑5 (MQ=255)
                                                                                                                     cATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCgccca                                                      >  2:1326249/1‑125 (MQ=255)
                                                                                                                     cATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCGTCAAACCAGACCGCCgcgc                                 >  2:1900102/1‑150 (MQ=255)
                                                                                                                       ttCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCGTCAAACCAGACCGCCGCGCTa                               >  2:378475/1‑150 (MQ=255)
                                                                                                                              cATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGAGAAGTCGACAAGATCGACCCCGACGGCGGCAAACCAGATCGCCGCGCTATCTTccc                        >  2:529128/1‑150 (MQ=255)
                                                                                                                                  ggCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCGTCAAACCAGACCGCCGCGCTATCTTCCcggcg                    <  2:2261306/150‑1 (MQ=255)
                                                                                                                                  ggCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCGTCAAACCAGACCGCCGCGCTATCTTCCcggcg                    >  1:1057084/1‑150 (MQ=255)
                                                                                                                                           aTCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCGTCAAACCAGACCGCCGCGCTATCTTCCCGGCGGGCTTGGCg           >  1:2129162/1‑150 (MQ=255)
                                                                                                                                            tCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCt                                                                                             <  2:1882571/67‑1 (MQ=255)
                                                                                                                                            tCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCGTCAAACCAGACCGCCGCGCTATCTTCCCGGCGGGCTTg              >  1:1202121/1‑146 (MQ=255)
                                                                                                                                            tCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCGTCAAACCAGACCGCCGCGCTATCTTCCCGGCGGGCTTg              <  2:1202121/146‑1 (MQ=255)
                                                                                                                                            cccaGGCCGCCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCGTCAAACCAGACCGCCGCGCTATCTTCCCGGCGGGCTTGGCGa          >  2:2037470/4‑150 (MQ=255)
                                                                                                                                              aaGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACaa                                                                     <  2:2255320/89‑1 (MQ=255)
                                                                                                                                              aaGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACaa                                                                     >  1:2255320/1‑89 (MQ=255)
                                                                                                                                              aaGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCGTCAAACCAGACCGCCGCGCTATCTTCCCGGCGGGCTTGGCGAtt        >  1:1916906/1‑150 (MQ=255)
                                                                                                                                              aaGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCGTCAAACCAGACCGCCGCGCTATCTTCCCGGCGGGCTTGGCGAtt        >  2:943166/1‑150 (MQ=255)
                                                                                                                                                    ggcggcCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCGTCAAACCAGACCGCCGCGCTATCTTCCCGGCGGGCTTGGCGATTCTCGaa  <  2:428980/150‑1 (MQ=255)
                                                                                                                                                    ggcggcCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCGTCAAACCAGACCGCCGCGCTATCTTCCCGGCGGGCTTGGCGATTCTCGaa  <  2:666783/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AAGATCACCCCGGCCCGCTATGCCCAGGCCTACACTGCCGCACGCCTGGAACTGATGAGCATCGAAAATGCCCTGCACCGCCTGACCTGGGATGAGGCTATCGG‑CTCATCCGGCACCATTCGCGCCATCGGCGCGGCCATCAAGGCCGGCGGCCTGGGCAATGGCGAGGTCAACGCCGAAGGCCTGGCCTGGGTCAAACGCAAGCTGTTCAAGCTGGGCGAAGTCGACAAGATCGACTTCGACGGCGTCAAACCAGACCGCCGCGCTATCTTCCCGGCGGGCTTGGCGATTCTCGAA  >  NC_002947/5949880‑5950176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑