BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,961,105	C→T	5.0%	P58L (CCC→CTC)	PP_5226 →	Lppl family lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,961,105	0	C	T	5.0%	296.2 / 3.7	99	P58L (CCC→CTC)	PP_5226	Lppl family lipoprotein
Reads supporting (aligned to +/- strand): ref base C (48/46); new base T (2/3); total (50/49)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

Only 100 of 102 total aligned reads displayed.

CGCGGCGCTGGTCGCTGTTGCCTGCCTCGTTTCGGCCTGTGGCCAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCGGCCATTGCTGAACGTTACGGCACCCCCACCTACGTG  >  NC_002947/5960956‑5961253

cgcgGCGCTGGTCGCTGTTGCCTGCCTCGTTTCGGCCTGTGGCCAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGcc                                                                                                                                                      <  2:1218360/150‑1 (MQ=255)

     cgcTGGTCGCTGTTGCCTGCCTCGTTTCGGCCTGTGGCCAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGc                                                                                                                                                 <  2:1271897/150‑1 (MQ=255)

          gTCGCTGTTGCCTGCCTCGTTTCGGCCTGTGGCCAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCag                                                                                                                                            >  1:2387794/1‑150 (MQ=255)

             gCTGTTGCCTGCCTCGTTTCGGCCTGTGGCCAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCTCGGGCTGCtgttc                                                                                                                                         <  1:1336640/150‑3 (MQ=255)

             gCTGTTGCCTGCCTCGTTTCGGCCTGTGGCCAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCagtct                                                                                                                                         <  2:1652619/150‑2 (MQ=255)

               tGTTGCCTGCCTCGTTTCGGCCTGTGGCCAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCagtccga                                                                                                                                       <  2:2254334/150‑1 (MQ=255)

               tGTTGCCTGCCTCGTTTCGGCCTGTGGCCAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCagtccga                                                                                                                                       <  1:56133/150‑1 (MQ=255)

               tGTTGCCTGCCTCGTTTCGGCCTGTGGCCAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACAAGCCAAGATCCAAGCCGTCCCCGGCGCAAGAACAGCAGCCCGAGCTGCagtccga                                                                                                                                       >  2:1475511/1‑150 (MQ=255)

                    cctgcctCGTTTCGGCCTGTGGCCAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCagtccgagtccg                                                                                                                                  >  2:2328091/1‑150 (MQ=255)

                    cctgcctCGTTTCGGCCTGTGGCCAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCagtccgagt                                                                                                                                     >  2:1066303/1‑147 (MQ=255)

                    cctgcctCGTTTCGGCCTGTGGCCAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCagtccgagt                                                                                                                                     <  1:1066303/147‑1 (MQ=255)

                     ctgcctCGTTTCGGCCTGTGGCCAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAACGTCAATCTGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAgtccgagtccga                                                                                                                                 <  1:1268042/150‑1 (MQ=255)

                         ctCGTTTCGGCCTGTGGCCAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTccgagccc                                                                                                                             >  1:1084602/1‑150 (MQ=255)

                          tCGTTTCGGCCTGTGGCCAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTccgagcccg                                                                                                                            >  2:2098939/1‑150 (MQ=255)

                           cGTTTCGGCCTGTGGCCAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTccgagcccga                                                                                                                           <  1:927722/150‑1 (MQ=255)

                           cGTTTCGGCCTGTGGCCAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTccgagcccga                                                                                                                           <  1:511731/150‑1 (MQ=255)

                                   ccTGTGGCCAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGc                                                                                                                   <  2:1588777/150‑1 (MQ=255)

                                    cTGTGGCCAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGcc                                                                                                                  >  2:1020580/1‑150 (MQ=255)

                                     tgtgGCCAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCa                                                                                                                 <  2:464690/150‑1 (MQ=255)

                                          ccAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGGCAAGCCGGCCCCGGCACAAGCACAGCAGCCCGAGCTACAGTCCGAGGCCGAGCCCGAGCAGTCGCCAGCGCa                                                                                                            <  2:883951/150‑1 (MQ=255)

                                          ccAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCa                                                                                                            >  1:1546357/1‑150 (MQ=255)

                                          ccAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCAACAGCCCCAGCGGCAAGTTAGGCCGGCGCCTGCAGAAGATCAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCa                                                                                                            <  1:2098939/150‑1 (MQ=255)

                                           cAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAg                                                                                                           >  2:647754/1‑150 (MQ=255)

                                            aGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGt                                                                                                          >  1:1628798/1‑150 (MQ=255)

                                             gAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTa                                                                                                         >  1:1840202/1‑150 (MQ=255)

                                               aaGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAg                                                                                                       <  1:1506262/150‑1 (MQ=255)

                                               aaGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAg                                                                                                       <  1:2328091/150‑1 (MQ=255)

                                                 ggCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGa                                                                                                     <  2:1016949/150‑1 (MQ=255)

                                                   cccGCTGTATCTGCCTGAAGATGGCGATAACGGAAAAACGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGaaa                                                                                                   >  1:1769469/1‑150 (MQ=255)

                                                    ccGCTGTATCTGCCTGAATATGGCGATAACAGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCTAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAAc                                                                                                  <  2:918653/150‑1 (MQ=255)

                                                      gCTGTATCTGCCTTAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCa                                                                                                <  1:342595/150‑1 (MQ=255)

                                                      gCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCa                                                                                                <  2:2387794/150‑1 (MQ=255)

                                                        tGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGa                                                                                              <  2:9163/150‑1 (MQ=255)

                                                        tGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGa                                                                                              <  1:647754/150‑1 (MQ=255)

                                                            tCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGaa                                                                                          >  1:831440/1‑150 (MQ=255)

                                                              tGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAAGAGCAGCCCGAg                                                                                                                                                  >  1:1957274/1‑92 (MQ=255)

                                                              tGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAg                                                                                                                                                  <  2:1957274/92‑1 (MQ=255)

                                                                  tGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCa                                                                                                            <  2:2113144/126‑1 (MQ=255)

                                                                  tGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCa                                                                                                            >  1:2113144/1‑126 (MQ=255)

                                                                   gAAGATGGCGATAACGGCAAAGCGCACACGACGCACCGGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTc                                                                                   <  2:1769469/150‑1 (MQ=255)

                                                                      gATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAAc                                                                                >  2:549110/1‑150 (MQ=255)

                                                                       aTGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACt                                                                               >  1:1723014/1‑150 (MQ=255)

                                                                       aTGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACt                                                                               >  1:1465677/1‑150 (MQ=255)

                                                                       aTGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAAAAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACt                                                                               >  1:597803/1‑150 (MQ=255)

                                                                                aCGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACg                                                                      <  1:549110/150‑1 (MQ=255)

                                                                                  ggCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGt                                                                    >  1:1410888/1‑150 (MQ=255)

                                                                                  ggCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGt                                                                    >  1:1424575/1‑150 (MQ=255)

                                                                                   gCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAAAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTg                                                                   >  1:2103963/1‑150 (MQ=255)

                                                                                    cAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGa                                                                  <  2:1128146/150‑1 (MQ=255)

                                                                                              acGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTcgcg                                                        <  2:831440/150‑1 (MQ=255)

                                                                                              acGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTcgcg                                                        >  2:1322858/1‑150 (MQ=255)

                                                                                                 tCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGaa                                                     >  1:813888/1‑150 (MQ=255)

                                                                                                  cGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAg                                                    <  2:1084602/150‑1 (MQ=255)

                                                                                                    caccagcaccagCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGc                                                  >  1:199670/1‑150 (MQ=255)

                                                                                                    caccagcaccagCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGACCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGAAAGGCAATACCGGGAACGCTTTCAACAACCGCGACGGTGAGATGTTCGCGGAAGGc                                                  >  2:675865/1‑150 (MQ=255)

                                                                                                      ccagcaccagcCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGt                                                >  2:798510/1‑150 (MQ=255)

                                                                                                      ccagcaccagcCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGt                                                <  2:1410888/150‑1 (MQ=255)

                                                                                                       cagcaccagcCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAAc                                                                                                  >  1:2001703/1‑99 (MQ=255)

                                                                                                       cagcaccagcCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAAc                                                                                                  <  2:2001703/99‑1 (MQ=255)

                                                                                                        agcaccagcCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTgg                                              <  1:1486959/150‑1 (MQ=255)

                                                                                                          caccagcCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGcc                                            >  2:123729/1‑150 (MQ=255)

                                                                                                             cagcCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGAc                                                                       <  2:1414370/120‑1 (MQ=255)

                                                                                                             cagcCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGAc                                                                       >  1:1414370/1‑120 (MQ=255)

                                                                                                             cagcCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTg                                         >  2:294803/1‑150 (MQ=255)

                                                                                                               gcCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTc                                       >  1:557369/1‑150 (MQ=255)

                                                                                                                ccAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGc                                             <  2:773276/143‑1 (MQ=255)

                                                                                                                ccAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGc                                             >  1:773276/1‑143 (MQ=255)

                                                                                                                ccAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCg                                      <  2:1424575/150‑1 (MQ=255)

                                                                                                                 cAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCgg                                     >  1:1618359/1‑150 (MQ=255)

                                                                                                                 cAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCgg                                     >  1:458599/1‑150 (MQ=255)

                                                                                                                 cAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGACgg                                     >  1:553612/1‑150 (MQ=255)

                                                                                                                   aaaGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCGGcc                                   >  2:2009235/1‑150 (MQ=255)

                                                                                                                      gccaagccGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGccc                                           >  2:1526802/1‑139 (MQ=255)

                                                                                                                      gccaagccGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGccc                                           <  1:1526802/139‑1 (MQ=255)

                                                                                                                      gccaagccGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCGGCCAtt                                >  2:2157933/1‑150 (MQ=255)

                                                                                                                        caagccGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCGGCCATTGc                              >  1:779157/1‑150 (MQ=255)

                                                                                                                         aagccGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCGGCCATTGCt                             >  1:678583/1‑150 (MQ=255)

                                                                                                                          agccGGCCCCGGCACAAGAACAGCAGCCCGAGCCGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGa                                                                                              >  1:186627/1‑84 (MQ=255)

                                                                                                                          agccGGCCCCGGCACAAGAACAGCAGCCCGAGCCGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGa                                                                                              <  2:186627/84‑1 (MQ=255)

                                                                                                                               gCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCGGCCATTGCTGAACGt                       <  1:123729/150‑1 (MQ=255)

                                                                                                                                   cGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCGGCCATTGCTGAACGTTACg                   <  2:813888/150‑1 (MQ=255)

                                                                                                                                    ggCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCGGCCATTGCTGAACGTTACgg                  <  2:557369/150‑1 (MQ=255)

                                                                                                                                       acaAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCGGCCATTGCTGAACGTTACGGCAc               >  2:1042893/1‑150 (MQ=255)

                                                                                                                                         aaGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCttt                                                                                    <  1:330924/79‑1 (MQ=255)

                                                                                                                                         aaGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCttt                                                                                    >  2:330924/1‑79 (MQ=255)

                                                                                                                                         aaGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGGGTGGCCCTGTCGGCCGTTGCTGAACGTTACGGCAccc             >  1:403704/1‑150 (MQ=255)

                                                                                                                                         aaGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCTTGGCCCTGTCGGCCATTGCTGAACGTTACGGCAccc             >  1:1302017/1‑150 (MQ=255)

                                                                                                                                         aaGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCGGCCATTGCTGAACGTTAc                    >  2:2285308/1‑143 (MQ=255)

                                                                                                                                         aaGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCGGCCATTGCTGAACGTTAc                    <  1:2285308/143‑1 (MQ=255)

                                                                                                                                           gAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCGGCCATTGCTGAACGTTACGGCAccccc           <  2:1663199/150‑1 (MQ=255)

                                                                                                                                           aaaCAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCGGCCATTGCTGAACGTTACGGCAccccc           <  1:535363/149‑1 (MQ=255)

                                                                                                                                            gactgcagcTCGGGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCg                                      >  2:2193017/5‑122 (MQ=255)

                                                                                                                                            gactgcagcTCGGGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCg                                      <  1:2193017/118‑1 (MQ=255)

                                                                                                                                            gactgcagcTCGGGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCg                                      <  1:2193031/118‑1 (MQ=255)

                                                                                                                                            gactgcagcTCGGGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCg                                      >  2:2193031/5‑122 (MQ=255)

                                                                                                                                              cagcagCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCGGCCATTGCTGAACGTTACGGCa                <  2:777916/142‑1 (MQ=255)

                                                                                                                                              cagcagCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCGGCCATTGCTGAACGTTACGGCa                >  1:777916/1‑142 (MQ=255)

                                                                                                                                                 cagcCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCGGCCATTGCTGAACGTTACGGCACCCCCACCTAc     <  1:1322858/150‑1 (MQ=255)

                                                                                                                                                 caccccGAGCTGCAGTCCGAGTCCGGGTCCGAGCAGTCGCCAGCGCAGTAAGGAAGCCAGATGATCGCTTTCAACTACGGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCGGCCATTGCTGAACGTTACGGCTCCCCCTCCTAc     <  2:1546357/147‑1 (MQ=255)

                                                                                                                                                    cccGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCGGCCATTGCTGAACGTTACGGCACCCCCACCTACgtg  <  2:1628798/150‑1 (MQ=255)

CGCGGCGCTGGTCGCTGTTGCCTGCCTCGTTTCGGCCTGTGGCCAGAAAGGCCCGCTGTATCTGCCTGAAGATGGCGATAACGGCAAAGCGCACACGTCGCACCAGCACCAGCCAAAAGCCAAGCCGGCCCCGGCACAAGAACAGCAGCCCGAGCTGCAGTCCGAGTCCGAGCCCGAGCAGTCGCCAGCGCAGTAAGGAAACCAGATGAACGCTTTCAACTACCGCGACGGTGAGCTGTTCGCGGAAGGCGTGGCCCTGTCGGCCATTGCTGAACGTTACGGCACCCCCACCTACGTG  >  NC_002947/5960956‑5961253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`