BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,044,305	C→T	9.0%	V347V (GTG→GTA)	PP_5297 ←	amino acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,044,305	0	C	T	9.0%	280.1 / 13.0	102	V347V (GTG→GTA)	PP_5297	amino acid transporter
Reads supporting (aligned to +/- strand): ref base C (40/52); new base T (5/4); total (45/57)
Fisher's exact test for biased strand distribution p-value = 5.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 103 total aligned reads displayed.

GCAGATGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCGGTGAACAGGTA  >  NC_002947/6044161‑6044454

gcAGATGTGGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGCAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGc                                                                                                                                                  <  1:2298941/150‑1 (MQ=255)

  ataTGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGa                                                                                                                                                >  1:953305/3‑150 (MQ=255)

  aGATGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTGCTGGCCAGTACGGCgg                                                                                                                                                <  2:2340178/150‑2 (MQ=255)

   gATGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGAt                                                                                                                                               <  2:2368198/150‑1 (MQ=255)

    aTGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATg                                                                                                                                              >  2:1473024/1‑150 (MQ=255)

       gtggCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACGGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGAc                                                                                                                                           <  1:170340/150‑1 (MQ=255)

            aTAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCa                                                                                                                                       <  1:220996/149‑1 (MQ=255)

            aTAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCa                                                                                                                                       >  2:220996/1‑149 (MQ=255)

                cgcgGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATc                                                                                                                                  <  1:357489/150‑1 (MQ=255)

                 gcggcgTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGTGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATc                                                                                                                                        >  2:96045/1‑143 (MQ=255)

                 gcggcgTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGTGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATc                                                                                                                                        <  1:96045/143‑1 (MQ=255)

                  cggcgTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGc                                                                                                                                <  1:816739/150‑1 (MQ=255)

                    gcgTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCa                                                                                                                              <  1:1920977/150‑1 (MQ=255)

                      gTTTGCGAGCGCGGGCAGACCCTCGCGAA‑AAATACAGGTCCGCAACGGTGGTGACCCAGAGGCAGCAGTGTTCTCTCCGGCGCCACGAAGGTGGCCTGCAGCGAAGCCCCCCTTATGCCCAGCACGCAGCCGGACATCAGGAGTGCCAgt                                                                                                                            <  2:1729188/150‑2 (MQ=255)

                      gTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGc                                                                                                                            >  2:1431900/1‑150 (MQ=255)

                            gAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGcccc                                                                                                                      >  1:1922471/1‑150 (MQ=255)

                                gcgGGCAGACACTGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGGACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGa                                                                                                                  <  1:1949191/150‑1 (MQ=255)

                                   ggCATACACTGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCg                                                                                                               <  2:1210484/150‑1 (MQ=255)

                                   ggCAGACACTGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCg                                                                                                               <  2:761978/150‑1 (MQ=255)

                                    gCAGACACTGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCANGNCNAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCgg                                                                                                              >  1:1675565/1‑150 (MQ=255)

                                     cAGACACTGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCggg                                                                                                             <  1:367885/150‑1 (MQ=255)

                                            tGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGCCTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCa                                                                                                                                       >  1:163436/1‑117 (MQ=255)

                                            tGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGAt                                                                                                                                               <  1:1244199/109‑1 (MQ=255)

                                            tGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGAt                                                                                                                                               >  2:1244199/1‑109 (MQ=255)

                                            tGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCa                                                                                                                                       <  2:163436/117‑1 (MQ=255)

                                            tGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCa                                                                                                      >  2:1806020/1‑150 (MQ=255)

                                               atctcTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCATTTGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGt                                                                                                   <  1:633119/146‑1 (MQ=255)

                                                 aaCTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTgg                                                                                                 <  2:1763208/150‑1 (MQ=255)

                                                 aaCGTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTTCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTgg                                                                                                 <  2:848801/150‑1 (MQ=255)

                                                  aCTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTggg                                                                                                <  2:1922471/150‑1 (MQ=255)

                                                    ttCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCa                                                                                              >  1:505381/1‑150 (MQ=255)

                                                    ttCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCa                                                                                              >  1:1674674/1‑150 (MQ=255)

                                                    ttCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATACGGATCGCGAGCGGCCCCATTTCCGGTCCGGCCAGGTgagat                                                                                              >  2:633119/1‑146 (MQ=255)

                                                    ttCTCCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCa                                                                                              >  2:2088783/1‑150 (MQ=255)

                                                      cTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCaaa                                                                                            <  2:1449488/150‑1 (MQ=255)

                                                         cGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGAc                                                                                         <  1:553061/150‑1 (MQ=255)

                                                           gTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGGGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACACGATTATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGt                                                                                       >  1:1954963/1‑150 (MQ=255)

                                                            tACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGtt                                                                                      <  1:342333/150‑1 (MQ=255)

                                                              ccGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTGCTGGCCAGTACGGCggc                                                                                                                                               <  2:2234652/91‑3 (MQ=39)

                                                              ccGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTGCTGGCCAGTACGGCggc                                                                                                                                               >  1:2234652/1‑89 (MQ=39)

                                                                gC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTgg                                                                                                 >  2:1353027/1‑135 (MQ=255)

                                                                gC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTcc                                                                                  >  2:1010526/1‑150 (MQ=255)

                                                                gC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTcc                                                                                  >  1:69320/1‑150 (MQ=255)

                                                                gC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTcc                                                                                  >  1:1382329/1‑150 (MQ=255)

                                                                gC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCAGCTCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTgg                                                                                                 <  1:1353027/135‑1 (MQ=255)

                                                                   aCCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTggg                                                                                                >  2:1787963/1‑134 (MQ=255)

                                                                   aCCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTggg                                                                                                <  1:1787963/134‑1 (MQ=255)

                                                                   aCCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTg                                                                                >  2:1672391/1‑150 (MQ=255)

                                                                     cGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATg                                                                                                                                              <  1:1332486/86‑1 (MQ=255)

                                                                     cGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATg                                                                                                                                              >  2:1332486/1‑86 (MQ=255)

                                                                      gTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCt                                                                                 >  1:1597360/1‑146 (MQ=255)

                                                                      gTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGAt                                                                             <  1:1216802/150‑1 (MQ=255)

                                                                       taataaCCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACg                                                                                        <  1:1042148/138‑1 (MQ=255)

                                                                       taataaCCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACg                                                                                        >  2:1042148/1‑138 (MQ=255)

                                                                                aTGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGgttg                                                                   >  1:1924232/1‑150 (MQ=255)

                                                                                 tGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGgttgt                                                                  >  1:1515853/1‑150 (MQ=255)

                                                                                   gCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGAt                                                                             <  1:559834/137‑1 (MQ=255)

                                                                                   gCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGAt                                                                             >  2:559834/1‑137 (MQ=255)

                                                                                   gCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTc                                                                <  1:2284213/150‑1 (MQ=255)

                                                                                    ccAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCa                                                               >  2:260802/1‑150 (MQ=255)

                                                                                     cAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACCGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCg                                                                                                               >  2:1707691/1‑101 (MQ=255)

                                                                                     cAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACCGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCg                                                                                                               <  1:1707691/101‑1 (MQ=255)

                                                                                      aaCAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAgg                                                             >  1:481948/1‑150 (MQ=255)

                                                                                      aaCAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAgg                                                             <  2:505381/150‑1 (MQ=255)

                                                                                      aaCAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAgg                                                             <  2:1250300/150‑1 (MQ=255)

                                                                                      aaCAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAgg                                                             >  1:2271634/1‑150 (MQ=255)

                                                                                            gTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGa                                                       <  2:2263362/150‑1 (MQ=255)

                                                                                                   ccGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGttt                                                <  1:1473024/150‑1 (MQ=255)

                                                                                                    cGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTc                                               <  2:1515853/150‑1 (MQ=255)

                                                                                                      gcgcCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCAc                                             <  2:1455108/150‑1 (MQ=255)

                                                                                                      gcgcCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCAc                                             <  1:1971374/150‑1 (MQ=255)

                                                                                                         ccACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGAGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGa                                          <  1:1957721/150‑1 (MQ=255)

                                                                                                          cACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGAt                                         <  2:1674674/150‑1 (MQ=255)

                                                                                                              aaGGTGGACTGCAGCGACCAAGGCGGACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCg                                     <  2:1757834/150‑1 (MQ=255)

                                                                                                                        gCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCa                                                         <  2:748118/120‑1 (MQ=255)

                                                                                                                        gCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCa                                                         >  1:748118/1‑120 (MQ=255)

                                                                                                                         cAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAg                          >  2:1212500/1‑150 (MQ=255)

                                                                                                                               catcgccgTGCTGGCCAGTACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCAGTTTCACTGATACCg                                     >  1:2291505/4‑133 (MQ=255)

                                                                                                                               catcgccgTGCTGGCCAGTACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCAGTTTCACTGATACCg                                     <  2:2291505/130‑1 (MQ=255)

                                                                                                                                atcgccgTGCTGGCCAGTACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGa                                                       <  1:256656/112‑1 (MQ=255)

                                                                                                                                atcgccgTGCTGGCCAGTACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGa                                                       >  2:256656/3‑114 (MQ=255)

                                                                                                                                atcgccgTGCTGGCCAGTACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCg                   >  2:1938181/3‑150 (MQ=255)

                                                                                                                                 tcgccgTGCTGGCCAGTACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGa                  >  1:843841/2‑150 (MQ=255)

                                                                                                                                   gccgTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATg                >  1:211108/1‑150 (MQ=255)

                                                                                                                                    ccgTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATgg               <  2:1382329/150‑1 (MQ=255)

                                                                                                                                      gTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCg             <  2:1822334/150‑1 (MQ=255)

                                                                                                                                       tACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCa                                              >  1:1520633/1‑116 (MQ=255)

                                                                                                                                       tACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCa                                              <  2:1520633/116‑1 (MQ=255)

                                                                                                                                        aCTGGCCAGCACGGCGATGGACATCAGGAGCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCGGt           <  1:275613/150‑1 (MQ=255)

                                                                                                                                         cTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCGGTg          <  2:2271634/150‑1 (MQ=255)

                                                                                                                                         cTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCGGTg          >  2:268713/1‑150 (MQ=255)

                                                                                                                                          tGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCGGTGa         >  2:1298379/1‑150 (MQ=255)

                                                                                                                                            gCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCGGTGAAc       >  1:431445/1‑150 (MQ=255)

                                                                                                                                              cAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGAccc                                                    <  1:1170076/103‑1 (MQ=255)

                                                                                                                                              cAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGAccc                                                    >  2:1170076/1‑103 (MQ=255)

                                                                                                                                              cAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCGGTGAACAg     <  2:1924232/150‑1 (MQ=255)

                                                                                                                                              cAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCGGTGAACAg     <  2:953305/150‑1 (MQ=255)

                                                                                                                                                gCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCGGTGAACAGGt   <  1:1298379/150‑1 (MQ=255)

                                                                                                                                                gCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCGGTGAACAGGt   >  1:568434/1‑150 (MQ=255)

                                                                                                                                                 cACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCGGTGAACAGGTa  <  2:211108/150‑1 (MQ=255)

GCAGATGGTGGCATAGCGCGGCGTTTGCGAGCGCGGGCAGACACTGGCGAACTTCTGCGGTACCGC‑ACCGTAATAACCCATGGCCAACAGGGTGCGCGCCGGCGCCACGAAGGTGGACTGCAGCGACGCCGCCGTACTGGCCAGCACGGCGATGGACATCAGGATCGCCAGCGGCCCCATGACCGGGCCGGCCAGGTGGGCAAAGACGTTCTCCTGGATGCGCGGGTTGTTCAGGCCCAGACCCGTTTCACTGATACCGGCAAACTGCAGGGTGGCGATGGCGGTGAACAGGTA  >  NC_002947/6044161‑6044454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`