Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,116,752 G→A 5.3% R302H (CGT→CAT)  lpd → dihydrolipoyl dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,116,7520GA 5.3% 291.4 / 4.2 94R302H (CGT→CAT) lpddihydrolipoyl dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (48/41);  new base A (2/3);  total (50/44)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01

GAGCCTGCCGCCGGGGGTACCGCAGAATCGCTGCAAGCCGATTACGTGTTGGTGGCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCACGAGGTCAACTACAACCTCATCCCTGGCGTGAT  >  NC_002947/6116604‑6116899
                                                                                                                                                    |                                                                                                                                                   
gAGCCTGCCGCCGGGGGTACCGCAGAATCGCTGCAAGCCGATTACGTGTTGGTGGCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGt                                                                                                                                                    >  2:308235/1‑150 (MQ=255)
  gCCTGCCGCCGGGGGTACCGCAGAATCGCTGCAAGCCGATTACGTGTTGGTGGCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTAc                                                                                                                                                  <  2:1244029/150‑1 (MQ=255)
      gccgccGGGGGTACCGCAGAATCGCTGCAAGCCGATTACGTGTTGGTGGCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCa                                                                                                                                              <  2:256215/150‑1 (MQ=255)
                    cGCAGAATCGCTGCAAGCCGATTACGTGTTGGTGGCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTg                                                                                                                                >  1:152587/1‑150 (MQ=255)
                      cAGAATCGCTGCAAGCCGATTACGTGTTGGTGGCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTggg                                                                                                                              <  1:160668/150‑1 (MQ=255)
                      cAGAATCGCTGCAAGCCGATTACGTGTTGGTGGCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGCGGCGGTAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTggg                                                                                                                              >  1:761603/1‑150 (MQ=255)
                       aGAATCGCTGCAAGCCGATTACGTGTTGGTGGCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGt                                                                                                                             <  1:1571262/150‑1 (MQ=255)
                       aGAATCGCTGCAAGCCGATTACGTGTTGGTGGCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGt                                                                                                                             <  1:190163/150‑1 (MQ=255)
                       aGAATCGCTGCAAGCCGATTACGTGTTGGTGGCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGt                                                                                                                             <  1:2109754/150‑1 (MQ=255)
                               tGCAAGCCGATTACGTGTTGGTGGCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCg                                                                                                                     >  2:624727/1‑150 (MQ=255)
                                  aaGCCGATTACGTGTTGGTTGCTATCGGCCGTCGCCCTTATACAATAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACg                                                                                                                  <  2:1038542/150‑1 (MQ=255)
                                   aGCCGATTACGTGTTGGTGGCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGt                                                                                                                 >  1:2180704/1‑150 (MQ=255)
                                         ttACGTGTTGGTGGCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGt                                                                                                                 >  2:642759/1‑144 (MQ=255)
                                         ttACGTGTTGGTGGCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGt                                                                                                                 <  1:642759/144‑1 (MQ=255)
                                         ttACGTGTTGGTGGCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTc                                                                                                           <  1:624727/150‑1 (MQ=255)
                                            cGTGTTGGTGGCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCggg                                                                                                        <  2:1849237/150‑1 (MQ=255)
                                             gtgtTGGTGGCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGAc                                                                                                                   <  2:2251686/138‑1 (MQ=255)
                                             gtgtTGGTGGCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGAc                                                                                                                   >  1:2251686/1‑138 (MQ=255)
                                             gtgtTGGTGGCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGc                                                                                                       <  1:719571/150‑1 (MQ=255)
                                              tgtTGGTGGCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGcc                                                                                                      >  2:303288/1‑150 (MQ=255)
                                                   gtggCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATg                                                                                                 >  1:638709/1‑150 (MQ=255)
                                                        tATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTc                                                                                                                >  2:1971384/1‑130 (MQ=255)
                                                        tATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTc                                                                                                                <  1:1971384/130‑1 (MQ=255)
                                                        tATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGgc                                                                                            <  1:308235/150‑1 (MQ=255)
                                                           cGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGca                                                                                         >  1:359442/1‑150 (MQ=255)
                                                             gCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGcaca                                                                                       >  1:582763/1‑150 (MQ=255)
                                                              ccGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTg                                                                                                                        <  1:1922748/116‑1 (MQ=255)
                                                              ccGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTg                                                                                                                        >  2:1922748/1‑116 (MQ=255)
                                                                gTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGa                                                                                                                           <  1:1869771/111‑1 (MQ=255)
                                                                gTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGa                                                                                                                           >  2:1869771/1‑111 (MQ=255)
                                                                gTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAgg                                                                                    <  2:2112693/150‑1 (MQ=255)
                                                                gTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAgg                                                                                    >  1:526688/1‑150 (MQ=255)
                                                                gTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCATACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAgg                                                                                    <  2:2028458/150‑1 (MQ=255)
                                                                   gCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCg                                                                                 >  1:518544/1‑150 (MQ=255)
                                                                    cccTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCATACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGa                                                                                >  1:1597296/1‑150 (MQ=255)
                                                                     ccTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGCCCGGCTCCGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGaa                                                                               <  1:760339/150‑1 (MQ=255)
                                                                     ccTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTg                                                                                              >  2:2352874/1‑135 (MQ=255)
                                                                     ccTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTg                                                                                              <  1:2352874/135‑1 (MQ=255)
                                                                       ttATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGc                                                                                                                      <  1:1942560/109‑1 (MQ=255)
                                                                       ttATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGc                                                                                                                      >  2:1942560/1‑109 (MQ=255)
                                                                       ttATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGa                                                                             >  2:690379/1‑150 (MQ=255)
                                                                            cAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCATACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAg                                                                        <  2:1597296/150‑1 (MQ=255)
                                                                             aaaaGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGc                                                                       >  1:1826190/1‑150 (MQ=255)
                                                                                aGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGt                                                                    <  2:1826190/150‑1 (MQ=255)
                                                                                   gCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGGACAAGGCCGAAGACGAAGCGGTGGc                                                                 <  1:933622/150‑1 (MQ=255)
                                                                                   gCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGc                                                                 <  2:152587/150‑1 (MQ=255)
                                                                                   gCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGc                                                                 >  1:378523/1‑150 (MQ=255)
                                                                                     tAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCt                                                               >  1:1296757/1‑150 (MQ=255)
                                                                                     tAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCt                                                               >  1:2185554/1‑150 (MQ=255)
                                                                                        acgtGGAGAGCGTGGGCCGGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCGAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTa                                                            <  2:650985/147‑1 (MQ=255)
                                                                                        aCCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTa                                                            <  2:2207835/150‑1 (MQ=255)
                                                                                        aCCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTa                                                            <  1:1105611/150‑1 (MQ=255)
                                                                                          cTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATc                                                          >  2:1253982/1‑150 (MQ=255)
                                                                                          cTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGCTGTCTGGGTGATTAGAGACGTCACTTCGAGCCCAATGCTGGAACACAAGGCCGAAGACGAAGCGGTGGCCTGTATc                                                          >  2:811714/1‑150 (MQ=255)
                                                                                           tGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGgt                                                                                                                                     <  2:513299/74‑1 (MQ=255)
                                                                                           tGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGgt                                                                                                                                     >  1:513299/1‑74 (MQ=255)
                                                                                             gAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGaa                                                       <  2:582763/150‑1 (MQ=255)
                                                                                                  cGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCAt                                                  <  1:303288/150‑1 (MQ=255)
                                                                                                   gTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATc                                                 >  2:741318/1‑150 (MQ=255)
                                                                                                     gggCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGc                                               >  1:1734538/1‑150 (MQ=255)
                                                                                                         cTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCATACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCgg                                                                                                         >  1:2026656/1‑88 (MQ=255)
                                                                                                         cTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCATACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCgg                                                                                                         <  2:2026656/88‑1 (MQ=255)
                                                                                                                 cGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCa                                   <  1:741318/150‑1 (MQ=255)
                                                                                                                  gACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCAc                                  >  1:889836/1‑150 (MQ=255)
                                                                                                                  gACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCAc                                  <  1:811714/150‑1 (MQ=255)
                                                                                                                   aCAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCAc                                  >  1:783963/1‑149 (MQ=255)
                                                                                                                   aCAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCAc                                  <  2:783963/149‑1 (MQ=255)
                                                                                                                    cAAGCGAGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCACGa                                <  1:1912236/150‑1 (MQ=255)
                                                                                                                     aaGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCACGAg                               <  2:761603/150‑1 (MQ=255)
                                                                                                                      aGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCg                                                                                                                                        >  1:2114918/1‑44 (MQ=255)
                                                                                                                      aGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCg                                                                                                                                        <  2:2114918/44‑1 (MQ=255)
                                                                                                                          cgGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCACGAGGTCaa                          <  2:359442/150‑1 (MQ=255)
                                                                                                                            gCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCACGAGGTCAACt                        >  2:492158/1‑150 (MQ=255)
                                                                                                                                gCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCACGAGGTCAACTACaa                    >  1:1907881/1‑150 (MQ=255)
                                                                                                                                 cTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCAACGAGGTCAACTACAAc                   >  2:568159/1‑150 (MQ=255)
                                                                                                                                  tCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTATGTCGAACGCATCGCCGGCAAGCCCCACGAGGTAAAATACAAcc                  >  1:1719929/1‑150 (MQ=255)
                                                                                                                                   caacaaCGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCACGAGGTCAACTACAACCt                 >  2:1927713/1‑150 (MQ=255)
                                                                                                                                   caacaaCGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCACGAGGTCAACTACAACCt                 >  2:1926898/1‑150 (MQ=255)
                                                                                                                                    aacaacGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGcc                                                                <  2:202342/102‑1 (MQ=255)
                                                                                                                                    aacaacGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGcc                                                                >  1:202342/1‑102 (MQ=255)
                                                                                                                                       aacGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCgg                                                                     <  1:628284/94‑1 (MQ=255)
                                                                                                                                       aacGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCgg                                                                     >  2:628284/1‑94 (MQ=255)
                                                                                                                                       aacGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCACGAGGTCAACTACAACCTCATc             >  2:1318124/1‑150 (MQ=255)
                                                                                                                                        acGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCACGAGGTCAACTACAACCTCATcc            >  1:8474/1‑150 (MQ=255)
                                                                                                                                         cGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGgc                                                                                            >  1:2275870/1‑69 (MQ=255)
                                                                                                                                         cGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGgc                                                                                            <  2:2275870/69‑1 (MQ=255)
                                                                                                                                          gAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCACGAGGTCAACTACAACCTCATCCCt          >  2:1889304/1‑150 (MQ=255)
                                                                                                                                            gCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCACGAGGTCAACTACAACCTCATCCCTgg        >  2:1579576/1‑150 (MQ=255)
                                                                                                                                             caccacCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCACGAGGTCAACTACAACCTCATCCCTGGc       >  2:619909/1‑150 (MQ=255)
                                                                                                                                              accaccGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCACGAGGTCAACTACAACCTCATCCCTGGCg      >  2:40167/1‑150 (MQ=255)
                                                                                                                                                caccGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCACGAGGTCAACTACAACCTCATCCCTGGCGTg    >  2:971476/1‑150 (MQ=255)
                                                                                                                                                 accGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCACGAGGTCAACTACAACCTCATCCCTGGCGTGa   >  1:5067/1‑150 (MQ=255)
                                                                                                                                                  ccGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCACGAGGTCAACTACAACCTCATCCCTGGCGTGAt  <  2:2344783/150‑1 (MQ=255)
                                                                                                                                                  ccGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCACGAGGTCAACTACAACCTCATCCCTGGCGTGAt  <  1:971476/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAGCCTGCCGCCGGGGGTACCGCAGAATCGCTGCAAGCCGATTACGTGTTGGTGGCTATCGGCCGTCGCCCTTATACAAAAGGGCTAAACCTGGAAAGCGTGGGCCTGGAAGCCGACAAGCGCGGCATGCTCAACAACGAGCACCACCGTACCTCAGTGCCGGGTGTCTGGGTGATTGGCGACGTCACTTCGGGCCCAATGCTGGCGCACAAGGCCGAAGACGAAGCGGTGGCCTGTATCGAACGCATCGCCGGCAAGCCCCACGAGGTCAACTACAACCTCATCCCTGGCGTGAT  >  NC_002947/6116604‑6116899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑