Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 680,677 A→G 5.8% Q65R (CAG→CGG)  PP_0583 → AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947680,6770AG 5.8% 372.5 / 8.3 122Q65R (CAG→CGG) PP_0583AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (58/56);  new base G (4/3);  total (62/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.01e-01

Only 100 of 123 total aligned reads displayed.

CTGCCCAGACCCCTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCTGGCGCGCGAGC  >  NC_002947/680529‑680826
                                                                                                                                                    |                                                                                                                                                     
cTGCCCAGACCCCTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAg                                                                                                                                                      >  1:1238948/1‑150 (MQ=255)
   cccAGACCCCTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTgg                                                                                                                                                   >  1:1475514/1‑150 (MQ=255)
    ccAGACCCCTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTggg                                                                                                                                                  <  2:2374854/150‑1 (MQ=255)
    ccAGACCCCTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTggg                                                                                                                                                  <  2:957480/150‑1 (MQ=255)
          cccTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGgtgt                                                                                                                                            >  1:1013037/1‑150 (MQ=255)
           ccTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGtgtg                                                                                                                                           <  2:889677/150‑1 (MQ=255)
            ctctACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCCCCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTgg                                                                                                                                          >  2:1124149/1‑150 (MQ=255)
            ctctACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTgg                                                                                                                                          >  1:2016275/1‑150 (MQ=255)
             tctACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGtgtg                                                                                                                                           >  2:1409950/1‑148 (MQ=255)
             tctACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGtgtg                                                                                                                                           <  1:1409950/148‑1 (MQ=255)
             tctACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGa                                                                                                                                         >  2:1646003/1‑150 (MQ=255)
             tctACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGa                                                                                                                                         <  1:1450802/150‑1 (MQ=255)
              ctACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGAt                                                                                                                                        <  1:1646003/150‑1 (MQ=255)
               tACGCCCGTGCCGAAAGCTTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATc                                                                                                                                       <  2:1378468/150‑1 (MQ=255)
                    ccGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCccgc                                                                                                                                  <  2:1988666/150‑1 (MQ=255)
                       tGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGa                                                                                                                               >  1:1014548/1‑150 (MQ=255)
                           gAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGcc                                                                                                                           >  2:1294588/1‑150 (MQ=255)
                           gAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGc                                                                                                                            >  2:2290441/1‑149 (MQ=255)
                           gAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGc                                                                                                                            <  1:2290441/149‑1 (MQ=255)
                             aaGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCAGCCGATGGCGa                                                                                                                         <  2:2332446/150‑1 (MQ=255)
                                ccTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGgtgt                                                                                                                                            <  1:1366534/128‑1 (MQ=255)
                                ccTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGgggt                                                                                                                                            >  2:1366534/1‑128 (MQ=255)
                                 cTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCAt                                                                                                                     >  2:1378975/1‑150 (MQ=255)
                                  ttGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATg                                                                                                                    <  2:1013037/150‑1 (MQ=255)
                                    ggCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGaa                                                                                                                  <  1:1713750/150‑1 (MQ=255)
                                     gcgcCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAg                                                                                                                 <  1:1294588/150‑1 (MQ=255)
                                       gcCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTg                                                                                                               <  1:2335214/150‑1 (MQ=255)
                                          ggATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTc                                                                                                            <  1:500453/150‑1 (MQ=255)
                                          ggATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTc                                                                                                            <  1:438493/150‑1 (MQ=255)
                                             tCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTggg                                                                                                                                                  <  1:181546/109‑1 (MQ=255)
                                                   aCCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATg                                                                                                   >  2:1576661/1‑150 (MQ=255)
                                                    ccACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGc                                                                                                  >  2:962199/1‑150 (MQ=255)
                                                       cTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAg                                                                                               >  2:1014632/1‑150 (MQ=255)
                                                        tctccaccaGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTgg                                                                                                                                          >  2:2213333/4‑106 (MQ=255)
                                                        tCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTgg                                                                                                                                          <  1:2213333/106‑1 (MQ=255)
                                                        tCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCccgcactg                                                                                                                              <  1:2268382/118‑5 (MQ=255)
                                                        tCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGGAAGc                                                                                              >  1:409151/1‑150 (MQ=255)
                                                        tCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGc                                                                                              >  2:1990054/1‑150 (MQ=255)
                                                          gCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCg                                                                                            >  1:1879027/1‑150 (MQ=255)
                                                             accaGTACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCgaga                                                                                         >  1:1194931/1‑150 (MQ=255)
                                                             accaGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCgaga                                                                                         <  2:710150/150‑1 (MQ=255)
                                                                aGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATgc                                                                                      <  1:584573/150‑1 (MQ=255)
                                                                aGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATgc                                                                                      <  1:1418980/150‑1 (MQ=255)
                                                                aGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATgc                                                                                      >  2:1541232/1‑150 (MQ=255)
                                                                aGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATgc                                                                                      <  1:1990054/150‑1 (MQ=255)
                                                                 gCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCAACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATgcg                                                                                     >  2:1312005/1‑150 (MQ=255)
                                                                     gACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGc                                                                                 >  2:810103/1‑150 (MQ=255)
                                                                     gACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGAGGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGc                                                                                 <  1:1044018/150‑1 (MQ=255)
                                                                         gggTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCAACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGt                                                                             <  1:1312005/150‑1 (MQ=255)
                                                                           gTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTAc                                                                           <  2:1014548/150‑1 (MQ=255)
                                                                            tGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTc                                                                                                            >  1:1548580/1‑116 (MQ=255)
                                                                            tGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACg                                                                          >  2:2115161/1‑150 (MQ=255)
                                                                                aTTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCga                                                                                           <  2:176477/129‑1 (MQ=255)
                                                                                aTTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCga                                                                                           >  1:176477/1‑129 (MQ=255)
                                                                                      cTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATgc                                                                                      >  2:159131/1‑128 (MQ=255)
                                                                                      cTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGa                                                                >  2:126026/1‑150 (MQ=255)
                                                                                       tACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGgtgt                                                                                                                                            >  1:1130908/1‑73 (MQ=255)
                                                                                       tACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGgtgt                                                                                                                                            <  2:1130908/73‑1 (MQ=255)
                                                                                                  cggcgTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATg                                                    <  2:1238948/150‑1 (MQ=255)
                                                                                                   ggcgTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCAGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATgg                                                   <  1:1619229/150‑1 (MQ=255)
                                                                                                       tACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGgcgc                                               >  2:1034540/1‑150 (MQ=255)
                                                                                                            ggCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGc                                                                                                                       >  2:651794/1‑73 (MQ=255)
                                                                                                            ggCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGc                                                                                                                       <  1:651794/73‑1 (MQ=255)
                                                                                                            ggCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGaa                                          <  1:1042062/150‑1 (MQ=255)
                                                                                                                 gtACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATg                                     >  1:490580/1‑150 (MQ=255)
                                                                                                                  tACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGcc                                                                                >  2:716385/1‑106 (MQ=255)
                                                                                                                  tACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGcc                                                                                <  1:716385/106‑1 (MQ=255)
                                                                                                                     acacGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGAt                                                                                                    >  1:1469129/1‑83 (MQ=255)
                                                                                                                     acacGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGAt                                                                                                    <  2:1469129/83‑1 (MQ=255)
                                                                                                                     acacGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCgg                                 >  2:157808/1‑150 (MQ=255)
                                                                                                                         gcgACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCg                                  >  2:121055/1‑145 (MQ=255)
                                                                                                                         gcgACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCg                                  <  1:121055/145‑1 (MQ=255)
                                                                                                                          cgACGGCAGTTACTTTGCGCCGCCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCt                            <  1:126026/150‑1 (MQ=255)
                                                                                                                          cgACGGCAGTTACTTTGCCCCCCCGCCGCGGGGGGTGTGGGTCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCt                            <  1:539065/150‑1 (MQ=255)
                                                                                                                          cgACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCt                            <  2:1103818/150‑1 (MQ=255)
                                                                                                                           gACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTGCGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTg                           <  1:1014632/150‑1 (MQ=255)
                                                                                                                               gCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAg                       >  2:2210146/1‑150 (MQ=255)
                                                                                                                                cAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGt                      <  1:268006/150‑1 (MQ=255)
                                                                                                                                cAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGt                      <  1:1378975/150‑1 (MQ=255)
                                                                                                                                   ttACTTTGCCCGACCGCAGTGTGGGGTGAGGAACCCGGCCGCTGCGGGCCCTGAAGTGGTCACCTGGATGCAAGCCGTGATGCGCGGCCTGTACGTGCGCCGCGAGGGCTGACCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGAc                   <  2:1475514/150‑1 (MQ=255)
                                                                                                                                   ttACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCAt                                                                                                                     >  1:2210461/1‑52 (MQ=255)
                                                                                                                                   ttACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCAt                                                                                                                     <  2:2210461/52‑1 (MQ=255)
                                                                                                                                   ttACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGAc                   >  1:321365/1‑150 (MQ=255)
                                                                                                                                    ccacaccccccACTGCGGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGAc                   <  2:1829603/142‑1 (MQ=255)
                                                                                                                                       tttGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACGATGCCGGGTGCTGGaa                        >  1:257223/1‑141 (MQ=255)
                                                                                                                                       tttGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACGATGCCGGGTGCTGGaa                        <  2:257223/141‑1 (MQ=255)
                                                                                                                                       tttGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGAcgccg               >  2:1142754/1‑150 (MQ=255)
                                                                                                                                         tGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCt             <  2:2240323/150‑1 (MQ=255)
                                                                                                                                          cccccACTGCGGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGACGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCTg            >  1:1157299/2‑150 (MQ=255)
                                                                                                                                          cccccACTGCGGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCt                                                                                                        >  1:688183/2‑58 (MQ=255)
                                                                                                                                            cccACTGCGGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGCGCTGGaa                        >  2:1448391/1‑136 (MQ=255)
                                                                                                                                            cccACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCTGgc          >  1:972137/1‑150 (MQ=255)
                                                                                                                                             ccACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCTGgcg         >  1:2002966/1‑150 (MQ=255)
                                                                                                                                              cACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCGGGGACAATGGCGGGTGCTGGAAGTGACGCCGCGGgcgc        >  1:990913/1‑150 (MQ=255)
                                                                                                                                              cACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCTGgcgc        >  1:131736/1‑150 (MQ=255)
                                                                                                                                              cACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCTGgcgc        >  2:970598/1‑150 (MQ=255)
                                                                                                                                                 cGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCTg            >  1:988170/1‑143 (MQ=255)
                                                                                                                                                 cGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCTg            <  2:988170/143‑1 (MQ=255)
                                                                                                                                                  gCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCTGGCGCGCGa    <  1:2210146/150‑1 (MQ=255)
                                                                                                                                                    aGTGGGGGGTGTCGATCCCCGCCGATGCCGAGCATGAAGTCGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCTGGCGCGCGAGc  <  1:936618/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTGCCCAGACCCCTCTACGCCCGTGCCGAAAGCCTTGGCGCCGGATCGTGGACCACTCGCCACCAGCACGACTGGGTGCAATTCTCCTACGCCATCAGCGGCGTACTCGGCGTGTACACACGCGACGGCAGTTACTTTGCCCCACCGCAGTGGGGGGTGTGGATCCCCGCCGATGCCGAGCATGAAGTGGTCACCTCGATGCAAGCCGAGATGCGCAGCCTGTACGTGCGCCGCGATGCCTGCCCATGGGCGCCGGAACAATGCCGGGTGCTGGAAGTGACGCCGCTGGCGCGCGAGC  >  NC_002947/680529‑680826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑