Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	735,838	C→G	5.6%	G168R (GGC→CGC) ‡	PP_0628 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	735,838	0	C	G	5.6%	283.8 / 5.8	89	G168R (GGC→CGC) ‡	PP_0628	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (43/40);  new base G (3/2);  total (47/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

AAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGTAATTGTGCGCCTTCTTGCGCAAGGTGGGCGCCA  >  NC_002947/735691‑735987                                                                                                                                                    |                                                                                                                                                      aaaCAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCt                                                                                                                                                     >  2:654023/1‑150 (MQ=255)  aaCAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTg                                                                                                                                                    >  2:898274/1‑150 (MQ=255)      gacGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCACCAGCACTgcccc                                                                                                                                                >  2:261939/3‑146 (MQ=255)        cGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGGGCTGgcgctc                                                                                                                                              <  2:550082/150‑3 (MQ=255)           tGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTa                                                                                                                                           >  2:1127466/1‑150 (MQ=255)              aCTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTc                                                                                                                                        >  2:1489239/1‑150 (MQ=255)               cTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCg                                                                                                                                       <  2:1420968/150‑1 (MQ=255)               cTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCg                                                                                                                                       <  2:1878918/150‑1 (MQ=255)                tGGATCAAAGACTGATGCCTACGTCGCAGGCTATGTTGCCGCCCAGGTTGCCGCGCAGGAAATGCGGCGCATCGGGGGGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGTCAGCCCTGGCGCAGGTATTCgg                                                                                                                                      <  2:801628/150‑1 (MQ=255)                  gATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTa                                                                                                                                    >  2:2404787/1‑150 (MQ=255)                   aTCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAg                                                                                                                                   >  2:37293/1‑150 (MQ=255)                          aCTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGcggcgg                                                                                                                            >  2:1693023/1‑150 (MQ=255)                            tGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAGCAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGa                                                                                                                          >  2:1420811/1‑150 (MQ=255)                            tGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAggcatcagtc                                                                                                                          >  2:1778350/1‑143 (MQ=255)                            tGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGa                                                                                                                          >  1:1826811/1‑150 (MQ=255)                                  cTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGcggcgg                                                                                                                            <  2:787308/142‑1 (MQ=255)                                  cTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGcggcgg                                                                                                                            >  1:787308/1‑142 (MQ=255)                                  cTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTggg                                                                                                                    <  1:825841/150‑1 (MQ=255)                                  cTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTggg                                                                                                                    >  1:718656/1‑150 (MQ=255)                                   tACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAgg                                                                                                                                             <  1:1736727/124‑1 (MQ=255)                                   tACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAgg                                                                                                                                             >  2:1736727/1‑124 (MQ=255)                                   tACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGgt                                                                                                                   <  2:1032936/150‑1 (MQ=255)                                         gAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGtt                                                                                                                <  1:38016/147‑1 (MQ=255)                                         gAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGtt                                                                                                                >  2:38016/1‑147 (MQ=255)                                              cGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGa                                                                                                        <  2:1826811/150‑1 (MQ=255)                                               gATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGt                                                                                                                       <  1:401270/134‑1 (MQ=255)                                               gATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGt                                                                                                                       >  2:401270/1‑134 (MQ=255)                                               gATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAg                                                                                                       <  2:718656/150‑1 (MQ=255)                                                    tgcGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGt                                                                                                                       <  2:1258375/129‑1 (MQ=255)                                                    tgcGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGt                                                                                                                       >  1:1258375/1‑129 (MQ=255)                                                    tgcGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCAc                                                                                                    >  1:1894438/1‑148 (MQ=255)                                                    tgcGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCAc                                                                                                    <  2:1894438/148‑1 (MQ=255)                                                       ggCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTcgc                                                                                               <  2:2303917/150‑1 (MQ=255)                                                         cccAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCTACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCgcgc                                                                                             <  1:1489239/150‑1 (MQ=255)                                                          ccAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGa                                                                                                        <  2:1820560/138‑1 (MQ=255)                                                          ccAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGa                                                                                                        >  1:1820560/1‑138 (MQ=255)                                                          ccAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCt                                                                                            >  1:1163233/1‑150 (MQ=255)                                                              gTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAg                                                                                        <  1:1693023/150‑1 (MQ=255)                                                              gTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTCGAg                                                                                        <  2:2358116/150‑1 (MQ=255)                                                                   cgcgCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTa                                                                                                                                    <  1:1358271/101‑1 (MQ=255)                                                                   cgcgCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTa                                                                                                                                    >  2:1358271/1‑101 (MQ=255)                                                                   cgcgCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTgg                                                                                   >  2:1071045/1‑150 (MQ=255)                                                                   cgcgCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTgg                                                                                   >  1:1849560/1‑150 (MQ=255)                                                                   cgcgCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTCGAGGCTgg                                                                                   >  1:2078043/1‑150 (MQ=255)                                                                    gcgcAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGga                                                                                  <  2:1640005/150‑1 (MQ=255)                                                                          gAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAAcgc                                                                            >  1:1041520/1‑150 (MQ=255)                                                                            aaTCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCga                                                                          <  1:2381140/150‑1 (MQ=255)                                                                            aaTCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTCGGCCCGACCAGCGACAGCGCGCCCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCga                                                                          <  1:654023/150‑1 (MQ=255)                                                                             aTCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCgat                                                                         <  2:2250009/150‑1 (MQ=255)                                                                              tCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAAcgc                                                                            <  1:460812/146‑1 (MQ=255)                                                                              tCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAAcgc                                                                            >  2:460812/1‑146 (MQ=255)                                                                                cGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCgatgat                                                                      >  1:2251493/1‑150 (MQ=255)                                                                                   cgcATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTc                                                                   <  2:1163233/150‑1 (MQ=255)                                                                                       tCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGc                                                               >  2:681458/1‑150 (MQ=255)                                                                                          gggTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCg                                                                                                                                       <  2:553703/75‑1 (MQ=255)                                                                                          gggTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCg                                                                                                                                       >  1:553703/1‑75 (MQ=255)                                                                                            gTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGtt                                                          <  2:218519/150‑1 (MQ=255)                                                                                            gTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGtt                                                          <  1:2114867/150‑1 (MQ=255)                                                                                             tGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCCGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTc                                                         <  1:2191211/150‑1 (MQ=255)                                                                                             tGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGGATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGCCTGGAGAAAGAGCTGATCTCCGGcgtgttg                                                         >  2:137777/1‑144 (MQ=255)                                                                                                    aCAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCa                                                  >  1:583867/1‑150 (MQ=255)                                                                                                     cAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAg                                                 >  2:219512/1‑150 (MQ=255)                                                                                                      aGTACCCGAGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGc                                                >  2:283706/1‑150 (MQ=255)                                                                                                          cccGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCGAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCcgc                                            >  2:1392986/1‑150 (MQ=255)                                                                                                          cccGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCcgc                                            >  1:882730/1‑150 (MQ=255)                                                                                                               gCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTcc                                       <  1:1127466/150‑1 (MQ=255)                                                                                                               gCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTcc                                       <  2:1387115/150‑1 (MQ=255)                                                                                                                 aaaGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCg                                     >  2:930728/1‑150 (MQ=255)                                                                                                                   aGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGt                                   >  1:197060/1‑150 (MQ=255)                                                                                                                     gTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGTaa                                 >  2:514422/1‑150 (MQ=255)                                                                                                                     gTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGTaa                                 <  1:283706/150‑1 (MQ=255)                                                                                                                      ttGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGTAAt                                >  2:655363/1‑150 (MQ=255)                                                                                                                       tGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGTAAtt                               <  2:1494887/150‑1 (MQ=255)                                                                                                                         gCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGTAATtgt                             <  1:514422/150‑1 (MQ=255)                                                                                                                            cGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGTAATTGTgcg                          <  1:1420811/150‑1 (MQ=255)                                                                                                                              aCCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGTAATTGTGCGcc                        <  1:896256/150‑1 (MQ=255)                                                                                                                                   cgacagcgaGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGTAATTGTGCGCcttctt                   >  2:1134819/1‑150 (MQ=255)                                                                                                                                    gacagcgaGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGTAATTGTGCGCCTTCTTg                  <  2:1849560/150‑1 (MQ=255)                                                                                                                                     acagcgaGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGTAATTGTGCGCCTTCTTgc                 <  2:2251493/150‑1 (MQ=255)                                                                                                                                      cagcgaGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGTAATTGTGCGCCTTCTTgcg                >  1:1534736/1‑150 (MQ=255)                                                                                                                                         cgagcgCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGTAATTGTGCGCCTTCTTGCGCaa             >  2:976479/1‑150 (MQ=255)                                                                                                                                          ctgcgcCAGGGCTGGCGCGGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCga                                                                          >  2:230188/3‑88 (MQ=255)                                                                                                                                          ctgcgcCAGGGCTGGCGCGGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCga                                                                          <  1:230188/86‑1 (MQ=255)                                                                                                                                          ctgcgcCAGGGCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGTAATTGTGCGCCTTCTTGCGCAAg            >  1:471879/3‑150 (MQ=255)                                                                                                                                          ctgcgcCAGGGCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGTAATTGTGCGCCTTCTTGCGCAAg            >  1:1298972/3‑150 (MQ=255)                                                                                                                                            gcgcCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGTAATTGTGCGCCTTCTTGCGCAAGGt          <  1:930728/150‑1 (MQ=255)                                                                                                                                              gcCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGTAATTGTGCGCCTTCTTGCGCAAGGt          <  1:2259479/148‑1 (MQ=255)                                                                                                                                              gcCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGTAATTGTGCGCCTTCTTGCGCAAGGt          >  2:2259479/1‑148 (MQ=255)                                                                                                                                                cAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGTAATTGTGCGCCTTCTTGCGCAAGGTGGgc      >  2:167423/1‑150 (MQ=255)                                                                                                                                                    ccTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGTAATTGTGCGCCTTCTTGCGCAAGGTGGGCGCCa  <  2:583867/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                      AAACAGTCGCTGCACTGGATCAAAGACTGATGCCTACGTCGAAGACGATGCTGCGGCCCAGGTTGCCGCGCAGGAAATCCGGCGCATCGGGGTGGGCAAACAGTACCCGGGCAAAGGTTGGCCCGACCAGCGACAGCGAGCGCCAGCCCTGGCGCAGGTATTCGGTAGGCGGCGGGAAGTGGGTGTTGAGGTCGAGCACTTCGCGCTTGAGGCTGGAGAACGCGATGATGTCCAGCTCGTTCAGCTCCAGCCCGCGTTCCCGGTAATTGTGCGCCTTCTTGCGCAAGGTGGGCGCCA  >  NC_002947/735691‑735987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑