Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	801,792	G→A	5.4%	V92I (GTC→ATC)	obg →	GTPase Obg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	801,792	0	G	A	5.4%	270.7 / 4.3	94	V92I (GTC→ATC)	obg	GTPase Obg
Reads supporting (aligned to +/- strand):  ref base G (48/39);  new base A (3/2);  total (52/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

TCGGTGTACATGATTGCCGACGAAAACCTGAACACCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGCAACACCCGTTTCAAGTCGAGCACCAACCGTGCGCCGCGCCAGACC  >  NC_002947/801648‑801941                                                                                                                                                 |                                                                                                                                                      gcGGTGTGCATGATTGCGTATCAAAATCTGGACACCCTTGTCGACTACCGAGCCACCCGACACCATGAAGCCCAGCGGGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGCAGGGTTAAGACCTGTTCCTGCGCGTGCCGGTCGGc                                                                                                                                                  <  1:193495/149‑1 (MQ=255)   ggTGTACATGATTGCCGACGAAAACCTGAACACCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGcac                                                                                                                                                <  2:1207471/150‑1 (MQ=255)    gtgtACATGATTGCCGACGAAAACCTGAAGACCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGcacc                                                                                                                                               >  1:65881/1‑150 (MQ=255)    gtgtACATGATTGCCGACGAAAACCTGAACACCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGcacc                                                                                                                                               >  1:336407/1‑150 (MQ=255)       tACATGATTGCCGACGAAAACCTGAACACCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGt                                                                                                                                                      >  2:744227/1‑140 (MQ=255)       tACATGATTGCCGACGAAAACCTGAACACCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGt                                                                                                                                                      <  1:744227/140‑1 (MQ=255)        aCATGATTGCCGACGAAAACCTGAACACCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCg                                                                                                                                           >  2:1766712/1‑150 (MQ=255)         cATGATTGCCGACGAAAACCTGAACACCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGt                                                                                                                                          >  1:2118993/1‑150 (MQ=255)           tGATTGCCGACGAAAACCTGAACACCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGa                                                                                                                                        <  2:1637013/150‑1 (MQ=255)                  cgacgaAAACCTGAACACCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGc                                                                                                                                 >  1:662747/1‑150 (MQ=255)                  cgacgaAAACCTGAACACCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGc                                                                                                                                 >  2:1461324/1‑150 (MQ=255)                  cgacgaAAACCTGAACACCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTCTTACTGCGCTTGCCGGTCTGCACCACCGTGTTCGATGc                                                                                                                                 >  2:21787/1‑150 (MQ=255)                    acgaAAACCTGAACACCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCtt                                                                                                                               <  2:205076/150‑1 (MQ=255)                    acgaAAACCTGAACACCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCtt                                                                                                                               <  2:1864766/150‑1 (MQ=255)                     cgaAAACCTGAACACCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTc                                                                                                                              <  1:378736/150‑1 (MQ=255)                      gaAAACCTGAACACCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGACCGGcacgc                                                                                                                                              <  2:777985/133‑3 (MQ=255)                      gaAAACCTGAACACCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGACCGGcacgc                                                                                                                                              >  1:777985/1‑131 (MQ=255)                           ccTGAACACCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTATCAACGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGCTCGGCACCACCGTGATCGATGCTTCCACGCa                                                                                                                        >  1:723927/1‑150 (MQ=255)                           ccTGAACACCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCa                                                                                                                        <  1:826358/150‑1 (MQ=255)                               aaCACCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGaa                                                                                                                    >  2:1758530/1‑150 (MQ=255)                                 cacCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGt                                                                                                                  >  1:922170/1‑150 (MQ=255)                                   cccTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGa                                                                                                                <  2:1795936/150‑1 (MQ=255)                                    ccTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATg                                                                                                                                  <  1:1113121/131‑1 (MQ=255)                                    ccTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATg                                                                                                                                  >  2:1113121/1‑131 (MQ=255)                                      tGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCg                                                                                                             <  2:292701/150‑1 (MQ=255)                                        gTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACATGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCCTCCACGCAGGAAGTGATCGGt                                                                                                           <  1:1454108/150‑1 (MQ=255)                                            aCTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGAcc                                                                                                       <  1:1461324/150‑1 (MQ=255)                                              tACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTg                                                                                                     >  1:20709/1‑150 (MQ=255)                                              tACCGCGACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCGGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTg                                                                                                     <  1:1026075/150‑1 (MQ=255)                                                ccGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGACCGGcacgcgca                                                                                                                                           >  2:788889/1‑105 (MQ=255)                                                ccGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGACCGGcacgcgca                                                                                                                                           <  1:788889/110‑6 (MQ=255)                                                 cGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCTTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGACGTGATCGGTGACCTGGTc                                                                                                  >  1:197459/1‑150 (MQ=255)                                                    tACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCAcc                                                                                               <  2:65881/150‑1 (MQ=255)                                                         ccGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCg                                                                                                             >  1:934881/1‑131 (MQ=255)                                                         ccGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCg                                                                                                             <  2:934881/131‑1 (MQ=255)                                                         ccGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCgg                                                                                          >  1:2404006/1‑150 (MQ=255)                                                           gTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGTTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCAGGTCAccgatcact                                                                                        <  2:540039/150‑8 (MQ=255)                                                                cATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAg                                                                                   >  1:1395422/1‑150 (MQ=255)                                                                     aGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGAt                                                                              >  1:1189362/1‑150 (MQ=255)                                                                      gCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATg                                                                             >  1:1967026/1‑150 (MQ=255)                                                                      gCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATg                                                                             <  2:1189362/150‑1 (MQ=255)                                                                       cccAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATgg                                                                            >  2:1694645/1‑150 (MQ=255)                                                                       cccAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATgg                                                                            >  2:1331213/1‑150 (MQ=255)                                                                         cAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTc                                                                          >  2:156168/1‑150 (MQ=255)                                                                             gTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGt                                                                                                                                          <  1:1569401/82‑1 (MQ=255)                                                                             gTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGt                                                                                                                                          >  1:1559227/1‑82 (MQ=255)                                                                             gTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGt                                                                                                                                          >  2:1569401/1‑82 (MQ=255)                                                                             gTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGt                                                                                                                                          <  2:1559227/82‑1 (MQ=255)                                                                             gTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCgcgc                                                                      >  1:1217019/1‑150 (MQ=255)                                                                              tGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGAACGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGGCGCGCa                                                                     >  2:128578/1‑150 (MQ=255)                                                                                 cTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAgg                                                                  >  2:2147807/1‑150 (MQ=255)                                                                                 cTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAgg                                                                  >  2:615352/1‑150 (MQ=255)                                                                                 cTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAgg                                                                  >  2:936425/1‑150 (MQ=255)                                                                                     aaCGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCgg                                                                                                                                                   >  1:269518/1‑65 (MQ=255)                                                                                     aaCGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCgg                                                                                                                                                   <  2:269518/65‑1 (MQ=255)                                                                                     aaCGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAg                                                                                                                       <  1:809266/93‑1 (MQ=255)                                                                                     aaCGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAg                                                                                                                       >  2:809266/1‑93 (MQ=255)                                                                                      aCGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGtggct                                                             <  2:1876585/150‑1 (MQ=255)                                                                                        ggcggTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAGGCTGATGGTCGCGCAAGgtgggtgg                                                           >  1:32916/1‑150 (MQ=255)                                                                                        ggcggTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGAt                                                                              <  2:1061694/131‑1 (MQ=255)                                                                                        ggcggTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGAt                                                                              >  1:1061694/1‑131 (MQ=255)                                                                                         gcggTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCAccc                                                                                              <  2:865578/114‑1 (MQ=255)                                                                                         gcggTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCAccc                                                                                              >  1:865578/1‑114 (MQ=255)                                                                                         gcggTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGtggctggc                                                          <  1:1766712/150‑1 (MQ=255)                                                                                            gTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGc                                                                                         <  2:987626/116‑1 (MQ=255)                                                                                            gTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGc                                                                                         >  1:987626/1‑116 (MQ=255)                                                                                                 aCCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCtt                                                  <  2:1395422/150‑1 (MQ=255)                                                                                                  ccGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTg                                                 >  1:592923/1‑150 (MQ=255)                                                                                                  ccGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTg                                                 <  1:156168/150‑1 (MQ=255)                                                                                                   cGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTgg                                                >  2:1990541/1‑150 (MQ=255)                                                                                                    gACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGc                                               <  1:1331213/150‑1 (MQ=255)                                                                                                         cACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCtt                                                  >  1:458369/1‑142 (MQ=255)                                                                                                         cACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCtt                                                  <  2:458369/142‑1 (MQ=255)                                                                                                               cAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGTACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGCAACACCCGttt                                    <  1:21787/150‑1 (MQ=255)                                                                                                               cAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCGCCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGCAACACCCGttt                                    <  2:922170/150‑1 (MQ=255)                                                                                                                aagaagGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGCAACACCCGTTTc                                   >  1:375679/1‑150 (MQ=255)                                                                                                                aagaagGGTGAAGACCTGTTCCTGCGCGTACCCGTCGGCACCACCGTGACCGATGCTTCCACGCAGGAAGTGATCGGTGCCCTGCTCACCACCGGCCAGACGCCGAGGATCGCGCAAGTTGGCTAGCACGGGCAGGGCTACACCCgctcc                                   >  2:218858/1‑146 (MQ=255)                                                                                                                       gTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGCAACACCCGTTTCAAGTCGa                            <  2:2118993/150‑1 (MQ=255)                                                                                                                          aaGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGCAACACCCGTTTCAAGTCGAGCa                         >  1:1018307/1‑150 (MQ=255)                                                                                                                               cTGTTCCTGCGCGTGCCGGTCGGCACCNCCGTGATCGATGNTTCCACNCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGTCACGGGCTTGGCAACACCCGTTTCAAGTCGAGCACCAAc                    >  1:2021192/1‑150 (MQ=255)                                                                                                                               cTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGCAACACCCGTTTCAAGTCGAGCACCAAc                    >  2:1585056/1‑150 (MQ=255)                                                                                                                               cTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGCAACACCCGTTTCAAGTCGAGCACCAAc                    >  2:267559/1‑150 (MQ=255)                                                                                                                               cTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGCAACACCCGTTTCAAGTCGAGCACCAAc                    <  1:1694645/150‑1 (MQ=255)                                                                                                                               cTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTAGATGGCACTGGCTTTGCAACACCCGTTTCAAGTCGAGCACCAAc                    >  2:2199697/1‑150 (MQ=255)                                                                                                                                tGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACACCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGCAACACCCGTTTCAAGTCGAGCACCAAcc                   <  2:197459/150‑1 (MQ=255)                                                                                                                                 gTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGCAACACCCGTTTCAAGTCGa                            >  2:1113308/1‑140 (MQ=255)                                                                                                                                 gTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGCAACACCCGTTTCAAGTCGa                            <  1:1113308/140‑1 (MQ=255)                                                                                                                                     cTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGCAACACCCGTTTCAAGTCGAGCACCAACCGTgcg              >  1:122851/1‑150 (MQ=255)                                                                                                                                      tGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGCAACACCCGt                                      >  1:1326540/1‑125 (MQ=255)                                                                                                                                      tGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGCAACACCCGt                                      <  2:1326540/125‑1 (MQ=255)                                                                                                                                       ggcggTGCCGACCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACATGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAACGTGGCTGGCAAGAGCTTGGCCACACCCGTTTCAAGTCGAGCACCACCCGTgcgca            >  2:615919/5‑149 (MQ=255)                                                                                                                                         gcgTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAANTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTAAGTCGTGCTGGCTTGGCAACACCCGTTTCAAGTCGAGCACCAACCGTgcgccgc          >  1:150174/1‑150 (MQ=255)                                                                                                                                         gcgTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGCAACACCCGTTTCAAGTCGAGCACCAACCGTgcgccgc          <  2:375679/150‑1 (MQ=255)                                                                                                                                             gCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGCAACACCCGTTTCAAGTCGAGCACCAACCGTGCGCCGCGCCa      <  1:1589193/150‑1 (MQ=255)                                                                                                                                                ggTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGCAACACCCGTTTCAAGTCGAGCACCAACCGTGCGCCGCGCCAGac   <  1:218858/150‑1 (MQ=255)                                                                                                                                                 gTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGCAACACCCGTTTCAAGTCGCGCACCAACCGTGCGCCGCGCCAGacc  >  2:436828/1‑150 (MQ=255)                                                                                                                                                 gTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGCAACACCCGTTTCAAGTCGAGCACCAACCGTGCGCCGCGCCAGacc  >  2:853756/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                      TCGGTGTACATGATTGCCGACGAAAACCTGAACACCCTGGTCGACTACCGCTACACCCGTCACCATGAAGCCCAGCGTGGCTCCAACGGCGGTAGCACCGACTGCACCGGCAAGAAGGGTGAAGACCTGTTCCTGCGCGTGCCGGTCGGCACCACCGTGATCGATGCTTCCACGCAGGAAGTGATCGGTGACCTGGTCACCCCCGGCCAGAAGCTGATGGTCGCGCAAGGTGGCTGGCACGGGCTTGGCAACACCCGTTTCAAGTCGAGCACCAACCGTGCGCCGCGCCAGACC  >  NC_002947/801648‑801941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑