Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 870,233 G→T 11.5% R65S (CGC→AGC)  PP_5456 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947870,2330GT11.5% 147.7 / 9.1 52R65S (CGC→AGC) PP_5456hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (24/22);  new base T (4/2);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

ATCGGCGAGTGCGGGGCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCGGGC  >  NC_002947/870084‑870374
                                                                                                                                                     |                                                                                                                                             
aTCGGCGAGTGCGGGGCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGcgcg                                                                                                                                               >  2:2342255/1‑150 (MQ=255)
aTCGGCGAGTGCGGGGCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGcgcg                                                                                                                                               >  1:2172523/1‑150 (MQ=255)
   ggCGAGTGCGGGGCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAgc                                                                                                                                            <  1:1505097/150‑1 (MQ=255)
        gTGCGGGGCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCaaa                                                                                                                                       <  1:1927038/150‑1 (MQ=255)
            ggggCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCTCGCGCAAAccgc                                                                                                                                   <  2:53832/150‑3 (MQ=255)
            ggggCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCg                                                                                                                                   >  2:1610535/1‑150 (MQ=255)
               gCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGc                                                                                                                                <  2:913501/150‑1 (MQ=255)
                ccctagcAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCt                                                                                                                               >  1:2154110/7‑150 (MQ=255)
                      cAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTcggcg                                                                                                                         <  2:2172523/150‑1 (MQ=255)
                       aGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAACCCCGGGCTTcggcgg                                                                                                                        <  2:421606/150‑1 (MQ=255)
                       aGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTcggcgg                                                                                                                        <  2:1551829/150‑1 (MQ=255)
                         gagaCGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGc                                                                                                                      >  1:1129178/1‑150 (MQ=255)
                                ccTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTcgc                                                                                                               >  2:1491714/1‑150 (MQ=255)
                                 cTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCACCGGCGGTTTGCGGGAGCGCAAACCCGGGCTTCGGCGGGCAGCTcgcg                                                                                                              >  1:648648/1‑150 (MQ=255)
                                  ttATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTcgccc                                                                                                             >  2:611657/1‑148 (MQ=255)
                                               gagaTTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAgccaggcca                                                                                                <  1:1610535/150‑1 (MQ=255)
                                                 gaTTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTGTGCCAGCCTTTTTCAGGCAGCGAGGAGGTGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGc                                                                                              <  1:2342255/150‑1 (MQ=255)
                                                         cGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATCGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTc                                                                                      <  1:1256516/150‑1 (MQ=255)
                                                              tCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGa                                                                                 >  2:345543/1‑150 (MQ=255)
                                                                 ggggCAGAGAGGTGGCCAGTGGGGGTTTGTGTGGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCt                                                                              <  2:1151259/149‑1 (MQ=255)
                                                                     cAGAAATGTCGCCATTCGTGGTTTTTATTCCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCAGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCAc                                                                          <  1:1079135/150‑1 (MQ=255)
                                                                        aaaTGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCa                                                                       >  2:2208141/1‑150 (MQ=255)
                                                                        aaaTGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCa                                                                       >  2:1005461/1‑150 (MQ=255)
                                                                                ccATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGc                                                               >  1:1165375/1‑150 (MQ=255)
                                                                                         gTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCTATGCCCCgtggtg                                                      >  2:1946539/1‑150 (MQ=255)
                                                                                         gTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCgtggtg                                                      >  1:1907522/1‑150 (MQ=255)
                                                                                           ttttATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGcc                                                    <  1:2395967/150‑1 (MQ=255)
                                                                                              tATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTgg                                                 <  1:1491714/150‑1 (MQ=255)
                                                                                                   cAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCa                                            >  1:957631/1‑150 (MQ=255)
                                                                                                   cAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCa                                            >  2:1436308/1‑150 (MQ=255)
                                                                                                        tttGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCt                                       >  2:1313250/1‑150 (MQ=255)
                                                                                                            tCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTTGATGCCCCGTGGTGCCTGGCTGCATTGCt                                       <  1:317796/146‑1 (MQ=255)
                                                                                                            tCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCACCGTGGTGCCTGGCTGCATTGCt                                       >  2:317796/1‑146 (MQ=255)
                                                                                                             cAGGCAGCGAGGAGGCGCTGGTGTGTTCTTGGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAg                                  <  1:611657/150‑1 (MQ=255)
                                                                                                             cAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAg                                  <  2:1165375/150‑1 (MQ=255)
                                                                                                             cAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAg                                  <  1:2146908/150‑1 (MQ=255)
                                                                                                                gCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGcc                               >  1:1188822/1‑150 (MQ=255)
                                                                                                                  aGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAg                             >  2:1112016/1‑150 (MQ=255)
                                                                                                                     gaggagGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGc                                                     >  2:376799/1‑123 (MQ=255)
                                                                                                                     gaggagGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGc                                                     <  1:376799/123‑1 (MQ=255)
                                                                                                                         aggCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTg                      <  2:1188822/150‑1 (MQ=255)
                                                                                                                               tGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATcc                >  2:199060/1‑150 (MQ=255)
                                                                                                                                  tGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCAtt                                          <  2:833267/121‑1 (MQ=255)
                                                                                                                                  tGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCAtt                                          >  1:833267/1‑121 (MQ=255)
                                                                                                                                   gCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGt            >  1:1027547/1‑150 (MQ=255)
                                                                                                                                     cAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCa          >  2:404116/1‑150 (MQ=255)
                                                                                                                                       aCGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAg        >  2:2409820/1‑150 (MQ=255)
                                                                                                                                         ccgggTTTGCGCTCGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGc      >  2:654518/4‑150 (MQ=255)
                                                                                                                                           cggTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCgg    <  2:957631/150‑1 (MQ=255)
                                                                                                                                            ggTTTGCGCTCGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCggg   >  2:2052683/1‑150 (MQ=255)
                                                                                                                                            ggTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCggg   <  2:1027547/150‑1 (MQ=255)
                                                                                                                                             gTTTGCGCTCGCGCAAACCCGGGCTTCGGCGGGCAGCTCgcgc                                                                                                             <  1:186595/43‑1 (MQ=25)
                                                                                                                                             gTTTGCGCTCGCGCAAACCCGGGCTTCGGCGGGCAGCTCgcgc                                                                                                             >  2:186595/1‑43 (MQ=25)
                                                                                                                                             gTTTGCGCTCGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCGGGc  >  2:1849039/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                             
ATCGGCGAGTGCGGGGCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCGGGC  >  NC_002947/870084‑870374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑