Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	870,234	A→C	11.5%	A64A (GCT→GCG)	PP_5456 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	870,234	0	A	C	11.5%	130.2 / 10.2	52	A64A (GCT→GCG)	PP_5456	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (24/22);  new base C (4/2);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

GGCGAGTGCGGGGCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCGGGCATAAGCGTT  >  NC_002947/870087‑870383                                                                                                                                                    |                                                                                                                                                      ggCGAGTGCGGGGCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAgc                                                                                                                                                     <  1:1505097/150‑1 (MQ=255)      gTGCGGGGCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCaaa                                                                                                                                                <  1:1927038/150‑1 (MQ=255)          ggggCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCTCGCGCAAAccgc                                                                                                                                            <  2:53832/150‑3 (MQ=255)          ggggCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCg                                                                                                                                            >  2:1610535/1‑150 (MQ=255)             gCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGc                                                                                                                                         <  2:913501/150‑1 (MQ=255)              ccctagcAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCt                                                                                                                                        >  1:2154110/7‑150 (MQ=255)                    cAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTcggcg                                                                                                                                  <  2:2172523/150‑1 (MQ=255)                     aGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAACCCCGGGCTTcggcgg                                                                                                                                 <  2:421606/150‑1 (MQ=255)                     aGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTcggcgg                                                                                                                                 <  2:1551829/150‑1 (MQ=255)                       gagaCGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGc                                                                                                                               >  1:1129178/1‑150 (MQ=255)                              ccTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTcgc                                                                                                                        >  2:1491714/1‑150 (MQ=255)                               cTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCACCGGCGGTTTGCGGGAGCGCAAACCCGGGCTTCGGCGGGCAGCTcgcg                                                                                                                       >  1:648648/1‑150 (MQ=255)                                ttATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTcgccc                                                                                                                      >  2:611657/1‑148 (MQ=255)                                             gagaTTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAgccaggcca                                                                                                         <  1:1610535/150‑1 (MQ=255)                                               gaTTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTGTGCCAGCCTTTTTCAGGCAGCGAGGAGGTGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGc                                                                                                       <  1:2342255/150‑1 (MQ=255)                                                       cGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATCGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTc                                                                                               <  1:1256516/150‑1 (MQ=255)                                                            tCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGa                                                                                          >  2:345543/1‑150 (MQ=255)                                                               ggggCAGAGAGGTGGCCAGTGGGGGTTTGTGTGGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCt                                                                                       <  2:1151259/149‑1 (MQ=255)                                                                   cAGAAATGTCGCCATTCGTGGTTTTTATTCCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCAGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCAc                                                                                   <  1:1079135/150‑1 (MQ=255)                                                                      aaaTGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCa                                                                                >  2:2208141/1‑150 (MQ=255)                                                                      aaaTGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCa                                                                                >  2:1005461/1‑150 (MQ=255)                                                                              ccATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGc                                                                        >  1:1165375/1‑150 (MQ=255)                                                                                       gTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCTATGCCCCgtggtg                                                               >  2:1946539/1‑150 (MQ=255)                                                                                       gTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCgtggtg                                                               >  1:1907522/1‑150 (MQ=255)                                                                                         ttttATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGcc                                                             <  1:2395967/150‑1 (MQ=255)                                                                                            tATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTgg                                                          <  1:1491714/150‑1 (MQ=255)                                                                                                 cAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCa                                                     >  1:957631/1‑150 (MQ=255)                                                                                                 cAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCa                                                     >  2:1436308/1‑150 (MQ=255)                                                                                                      tttGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCt                                                >  2:1313250/1‑150 (MQ=255)                                                                                                          tCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTTGATGCCCCGTGGTGCCTGGCTGCATTGCt                                                <  1:317796/146‑1 (MQ=255)                                                                                                          tCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCACCGTGGTGCCTGGCTGCATTGCt                                                >  2:317796/1‑146 (MQ=255)                                                                                                           cAGGCAGCGAGGAGGCGCTGGTGTGTTCTTGGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAg                                           <  1:611657/150‑1 (MQ=255)                                                                                                           cAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAg                                           <  2:1165375/150‑1 (MQ=255)                                                                                                           cAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAg                                           <  1:2146908/150‑1 (MQ=255)                                                                                                              gCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGcc                                        >  1:1188822/1‑150 (MQ=255)                                                                                                                aGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAg                                      >  2:1112016/1‑150 (MQ=255)                                                                                                                   gaggagGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGc                                                              <  1:376799/123‑1 (MQ=255)                                                                                                                   gaggagGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGc                                                              >  2:376799/1‑123 (MQ=255)                                                                                                                       aggCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTg                               <  2:1188822/150‑1 (MQ=255)                                                                                                                             tGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATcc                         >  2:199060/1‑150 (MQ=255)                                                                                                                                tGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCAtt                                                   <  2:833267/121‑1 (MQ=255)                                                                                                                                tGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCAtt                                                   >  1:833267/1‑121 (MQ=255)                                                                                                                                 gCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGt                     >  1:1027547/1‑150 (MQ=255)                                                                                                                                   cAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCa                   >  2:404116/1‑150 (MQ=255)                                                                                                                                     aCGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAg                 >  2:2409820/1‑150 (MQ=255)                                                                                                                                       ccgggTTTGCGCTCGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGc               >  2:654518/4‑150 (MQ=255)                                                                                                                                         cggTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCgg             <  2:957631/150‑1 (MQ=255)                                                                                                                                          ggTTTGCGCTCGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCggg            >  2:2052683/1‑150 (MQ=255)                                                                                                                                          ggTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCggg            <  2:1027547/150‑1 (MQ=255)                                                                                                                                           gTTTGCGCTCGCGCAAACCCGGGCTTCGGCGGGCAGCTCgcgc                                                                                                                      <  1:186595/43‑1 (MQ=25)                                                                                                                                           gTTTGCGCTCGCGCAAACCCGGGCTTCGGCGGGCAGCTCgcgc                                                                                                                      >  2:186595/1‑43 (MQ=25)                                                                                                                                           gTTTGCGCTCGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCGGGc           >  2:1849039/1‑150 (MQ=255)                                                                                                                                                    aGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGCAGGCCAGGCATTTGCCATTCGCGTCAAGGCGGGCATAAGCGtt  <  2:1129178/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                      GGCGAGTGCGGGGCCCGATCAGGAGACGTCCTTATAATCGGGGGGAGATTATAGCGATGTCGCGGGCAGAAATGTCGCCATTCGTGGTTTTTATTGCAGCGTTTGTCAGGCAGCGAGGAGGCGCTGGTGCCAACGGCGGTTTGCGCGAGCGCAAACCCGGGCTTCGGCGGGCAGCTCGCGCCCCAGCCAGGCCAGCTGAATTTCGCTGATCTGCACCCAGTCGATGCCCCGTGGTGCCTGGCTGCATTGCTTGAAGGCCAGGCATTTGCCATCCGCGTCAAGGCGGGCATAAGCGTT  >  NC_002947/870087‑870383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑