BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	873,428	T→C	8.7%	L20P (CTT→CCT)	PP_5457 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	873,428	0	T	C	8.7%	329.9 / 16.3	115	L20P (CTT→CCT)	PP_5457	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (46/59); new base C (3/7); total (49/66)
Fisher's exact test for biased strand distribution p-value = 5.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.85e-01

Only 100 of 116 total aligned reads displayed.

TTGCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGC‑CGAATGCGCACGGCAGCTGATCGATGCGTACAA  >  NC_002947/873280‑873569

ttGCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCtt                                                                                                                                               <  2:260157/150‑1 (MQ=255)

  gCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGc                                                                                                                                             <  2:180513/150‑1 (MQ=255)

   ccAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGcc                                                                                                                                            >  1:2379145/1‑150 (MQ=255)

    cAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAAGCATGCCTGCCa                                                                                                                                           <  2:857828/150‑1 (MQ=255)

    cAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAAGCATGCCTGCCa                                                                                                                                           <  2:1456480/150‑1 (MQ=255)

        tgtgCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGa                                                                                                                                       <  1:1917354/150‑1 (MQ=255)

         gtgCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAAGCATGCCTGCCAGcatt                                                                                                                                      <  2:1450864/150‑4 (MQ=255)

          tgCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAgg                                                                                                                                     >  2:583436/1‑150 (MQ=255)

           gCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGATTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAggg                                                                                                                                    <  1:121707/150‑1 (MQ=255)

            cTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAAGCATGCCTGCCAGcatttgc                                                                                                                                   <  1:1701576/150‑7 (MQ=255)

             tGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGa                                                                                                                                  <  1:2139693/150‑1 (MQ=255)

             tGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGa                                                                                                                                  >  1:2234957/1‑150 (MQ=255)

                 aGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGa                                                                                                                              >  1:525463/1‑150 (MQ=255)

                  gCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGAt                                                                                                                             <  1:1764370/150‑1 (MQ=255)

                  gCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGAt                                                                                                                             <  2:2063710/150‑1 (MQ=255)

                      gtggtgTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTac                                                                                                                         >  1:1824825/1‑150 (MQ=255)

                        ggtgTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTacac                                                                                                                       <  1:583436/150‑1 (MQ=255)

                        ggtgTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTacac                                                                                                                       <  2:525463/150‑1 (MQ=255)

                         gtgtGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACg                                                                                                                      >  1:670397/1‑150 (MQ=255)

                         gtgtGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACg                                                                                                                      >  2:384355/1‑150 (MQ=255)

                            tgCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCa                                                                                                                                           <  2:2026301/126‑1 (MQ=255)

                            tgCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCa                                                                                                                                           >  1:2026301/1‑126 (MQ=255)

                              cATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCa                                                                                                                                           <  1:2056034/124‑1 (MQ=255)

                              cATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTc                                                                                                                 <  2:2214969/150‑1 (MQ=255)

                                ttCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCgg                                                                                                               >  1:1569631/1‑150 (MQ=255)

                                ttCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCgg                                                                                                               <  1:158175/150‑1 (MQ=255)

                                ttCTGCAGGTGCATGGGGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCgg                                                                                                               <  2:205536/150‑1 (MQ=255)

                                  cTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGcc                                                                                                             <  1:827418/150‑1 (MQ=255)

                                      aGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGa                                                                                                         >  1:2137202/1‑150 (MQ=255)

                                          gCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCtt                                                                                                     >  1:1040690/1‑150 (MQ=255)

                                            aTGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCAGTGTCCCTGt                                                                                                   <  2:1300588/150‑1 (MQ=255)

                                             tGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGCCACCTGATGCAGCGTATCTACGGACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTTTc                                                                                                  >  1:257082/1‑150 (MQ=255)

                                             tGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTc                                                                                                  >  2:2378797/1‑150 (MQ=255)

                                                tGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAgg                                                                                                                                     >  1:1471637/1‑112 (MQ=255)

                                                  aCGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGcc                                                                                                             <  2:1782346/134‑1 (MQ=255)

                                                  aCGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGcc                                                                                                             >  1:1782346/1‑134 (MQ=255)

                                                  aCGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGt                                                                                               >  1:896870/1‑148 (MQ=255)

                                                   cGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTgcc                                                                                            >  2:513260/1‑150 (MQ=255)

                                                   cGAATGGATGCTTGAAGTCCGCCGCGAGGACGAAACCTGAGGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTgcc                                                                                            <  2:257082/150‑1 (MQ=255)

                                                          aTGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGAt                                                                                                                             <  2:768111/110‑1 (MQ=255)

                                                          aTGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGAt                                                                                                                             >  1:768111/1‑110 (MQ=255)

                                                           tGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGa                                                                                    <  2:1824825/150‑1 (MQ=255)

                                                              ttGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGAt                                                                                                                             >  2:1602044/1‑106 (MQ=255)

                                                              ttGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCt                                                                                 >  2:709972/1‑150 (MQ=255)

                                                              ttGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAAGCATGCCTGCCa                                                                                                                                           >  1:451507/1‑92 (MQ=255)

                                                               tGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGa                                                                                                                              >  2:406562/1‑104 (MQ=255)

                                                                  aGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGccc                                                                             <  2:670397/150‑1 (MQ=255)

                                                                    tCCGCCGCGAGGACGACACCTGATGCATCGTATCTATGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTacac                                                                                                                       <  1:1020909/106‑1 (MQ=255)

                                                                    tCCGCCGCGAGGACGACACCTGATGCAGCGTATCTATGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTacac                                                                                                                       >  2:1020909/1‑106 (MQ=255)

                                                                        ccgcGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAg                                                                       <  1:1899827/150‑1 (MQ=255)

                                                                         cgcgAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCCGGACCGCTGGCGAGTTAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAgg                                                                      <  2:1288898/150‑1 (MQ=255)

                                                                         cgcgAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAgg                                                                      <  2:1040690/150‑1 (MQ=255)

                                                                         cgcgAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAgg                                                                      <  1:1057100/150‑1 (MQ=255)

                                                                           tgAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGc                                                                    <  2:1097897/149‑1 (MQ=255)

                                                                                  gacACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTgg                                                             <  1:137802/150‑1 (MQ=255)

                                                                                    cacCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTgggg                                                           <  2:1569631/150‑1 (MQ=255)

                                                                                     acCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGAGCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGc                                                          >  2:1849896/1‑150 (MQ=255)

                                                                                       cTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCtc                                                        >  1:186172/1‑150 (MQ=255)

                                                                                        tGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCtcg                                                       >  2:1586288/1‑150 (MQ=255)

                                                                                        tGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCCAGCAGGCCCTGCAAGGGcagc                                                       <  1:841540/150‑4 (MQ=255)

                                                                                         gATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCtcgt                                                      <  1:1586288/150‑1 (MQ=255)

                                                                                          gtGCAGCGTATCTAAGAGCGGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCtcgtc                                                     <  2:621510/149‑1 (MQ=255)

                                                                                           tGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAAGCATGCCTGCCAGcatt                                                                                                                                      >  2:696408/1‑65 (MQ=37)

                                                                                           tGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAAGCATGCCTGCCAGcatt                                                                                                                                      <  1:696408/68‑4 (MQ=37)

                                                                                            gCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTcgtcgt                                                   <  2:2234957/150‑1 (MQ=255)

                                                                                              aGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTgg                                                 <  1:1018812/150‑1 (MQ=255)

                                                                                                  tATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCtga                                             <  1:384355/150‑1 (MQ=255)

                                                                                                   aTCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAgg                                                                      >  2:569206/1‑124 (MQ=255)

                                                                                                   aTCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAgg                                                                      <  1:569206/124‑1 (MQ=255)

                                                                                                      tACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAg                                         >  2:1889829/1‑150 (MQ=255)

                                                                                                      tACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAg                                         >  2:1935649/1‑150 (MQ=255)

                                                                                                      tACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAg                                         >  2:2221064/1‑150 (MQ=255)

                                                                                                         gAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAg                                      >  2:1526461/1‑150 (MQ=255)

                                                                                                          aaCCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAgg                                     <  2:1824482/150‑1 (MQ=255)

                                                                                                           aCCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGc                                    <  2:1920255/150‑1 (MQ=255)

                                                                                                              ggAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGC‑CGa                                >  2:452560/1‑150 (MQ=255)

                                                                                                                aaaGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGC‑CGAAt                              <  1:1935649/150‑1 (MQ=255)

                                                                                                                 aaGCCTGCTTGAGGCTCAAATGCTGGTATGCATTCTTGCCAGCTATGTGATCGATGTACACGTGGTCGTCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGTGCCTGCTGGGGCTCGTCGTGGCTGATGATCAGTC‑CTAATg                             <  2:186172/150‑1 (MQ=255)

                                                                                                                    ccTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGc                                    >  1:2251784/1‑141 (MQ=255)

                                                                                                                    ccTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGc                                    <  2:2251784/141‑1 (MQ=255)

                                                                                                                        cTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGC‑CGAATGCGCACgg                      <  2:662590/150‑1 (MQ=255)

                                                                                                                           gAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGC‑CGAATGCGCACGGCAg                   <  1:452560/150‑1 (MQ=255)

                                                                                                                           gAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGC‑CGAATGCGCACGGCAg                   <  1:2221064/150‑1 (MQ=255)

                                                                                                                             ggCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGC‑CGAATGCGCACGGCAGCt                 >  2:1984518/1‑150 (MQ=255)

                                                                                                                              gcgcAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGC‑CGAATGCGCACGGCAGCTg                >  1:2131225/1‑150 (MQ=255)

                                                                                                                              cgacAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGAGCTCGTCGTGGATGATG‑GCCGGCACGAAAGCGCACGCCAGCTg                >  1:1289727/4‑150 (MQ=255)

                                                                                                                                gcAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGC‑CGAATGCGCACGGc                     <  2:1633037/143‑1 (MQ=255)

                                                                                                                                gcAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGC‑CGAATGCGCACGGc                     >  1:1633037/1‑143 (MQ=255)

                                                                                                                                gcAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGC‑CGAATGCGCACGGCAGCTGAt              <  1:1526461/150‑1 (MQ=255)

                                                                                                                                 cAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGC‑CGAATGCGCACGGCAGCTGATc             <  1:2378797/150‑1 (MQ=255)

                                                                                                                                 cAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGC‑CGAATGCGCACGGCAGCTGATc             <  1:1889829/150‑1 (MQ=255)

                                                                                                                                       cTGGCAAGCATGCCTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTgggg                                                           >  1:308415/1‑99 (MQ=255)

                                                                                                                                       cTGGCAAGCATGCCTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTgggg                                                           <  2:308415/99‑1 (MQ=255)

                                                                                                                                        tGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTg                                                  >  1:1491495/1‑107 (MQ=255)

                                                                                                                                        tGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTg                                                  <  2:1491495/107‑1 (MQ=255)

                                                                                                                                        tGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGC‑CGAATGCGCACGGCAGCTGATc             >  2:2402102/1‑143 (MQ=255)

                                                                                                                                        tGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGC‑CGAATGCGCACGGCAGCTGATc             <  1:2402102/143‑1 (MQ=255)

                                                                                                                                          gcaggcaTGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGc                                                                    >  2:2186404/1‑87 (MQ=255)

                                                                                                                                          gcaggcaTGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGc                                                                    <  1:2186404/87‑1 (MQ=255)

                                                                                                                                          gcaggcaTGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCtgatg                                           >  1:639378/1‑112 (MQ=255)

TTGCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGC‑CGAATGCGCACGGCAGCTGATCGATGCGTACAA  >  NC_002947/873280‑873569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`