Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 894,409 T→C 5.6% V120V (GTT→GTC)  slyD → FKBP‑type peptidyl‑prolyl cis‑trans isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947894,4090TC 5.6% 277.4 / 4.0 89V120V (GTT→GTC) slyDFKBP‑type peptidyl‑prolyl cis‑trans isomerase
Reads supporting (aligned to +/- strand):  ref base T (41/43);  new base C (2/3);  total (43/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

CTGGCCGAACTGGTCAGCACCCTGAACCGTAGCCTGTTCGAAGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGCCATCACCACTGATTTTCTGCGCTAAGCTCAGA  >  NC_002947/894263‑894555
                                                                                                                                                  |                                                                                                                                                  
cTGGCCGAACTGGTCAGCACCCTGAACCGTAGCCTGTTCGAAGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCAGCGAGCCGGACGGTGACGTCGTCGCCGTCGAt                                                                                                                                                 <  1:1268899/150‑1 (MQ=255)
     cGAACTGGTCAGCACCCTGAACCGTAGCCTGTTCGAAGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGTGACGTCGTCGCCGTCGATgtcgc                                                                                                                                            <  1:525592/150‑5 (MQ=255)
     cGAACTGGTCAGCACCCTGAACCGTAGCCTGTTCGAAGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATgtcgc                                                                                                                                            <  1:944033/150‑5 (MQ=255)
      gAACTGGTCAGCACCCTGAACCGTAGCCTGTTCGAAGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAac                                                                                                                                           >  1:474354/1‑150 (MQ=255)
      gAACTGGTCAGCACCCTGAACCGTAGCCTGTTCGAAGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAac                                                                                                                                           <  1:736390/150‑1 (MQ=255)
       aaCTGGTCAGCACCCTGAACCGTAGCCTGTTCGAAGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAacc                                                                                                                                          >  1:754761/1‑150 (MQ=255)
            gTCAGCACCCTGAACCGTAGCCTGTTCGAAGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAAccaccca                                                                                                                                     >  1:978431/1‑150 (MQ=255)
                gCACCCTGAACCGTAGCCTGTTCGAAGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTgg                                                                                                                                 >  1:543153/1‑150 (MQ=255)
                   cccTGAACCGTAGCCTGTTCGAAGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCg                                                                                                                              <  2:474354/150‑1 (MQ=255)
                        aaCCGTAGCCTGTTCGAAGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAg                                                                                                                         >  1:111018/1‑150 (MQ=255)
                         aCCGTAGCCTGGTCGAAGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAgc                                                                                                                        <  2:1062309/150‑1 (MQ=255)
                               gCCTGTTCGAAGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGa                                                                                                                  >  1:208204/1‑150 (MQ=255)
                                ccTGTTCGAAGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAgcgc                                                                                                                      <  1:234973/145‑1 (MQ=255)
                                ccTGTTCGAAGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAgcgc                                                                                                                      >  2:234973/1‑145 (MQ=255)
                                 cTGTTCGAAGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGAcc                                                                                                                >  2:557210/1‑150 (MQ=255)
                                  tGTTCGAAGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCt                                                                                                               >  2:2266037/1‑150 (MQ=255)
                                  tGTTCGAAGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCt                                                                                                               >  2:1162258/1‑150 (MQ=255)
                                    ttCGAAGGTGTCGACGCGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCCGACGGCCAGATGCACATGTTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTc                                                                                                             <  1:286730/150‑1 (MQ=255)
                                      cGAAGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCa                                                                                                           <  1:2052596/150‑1 (MQ=255)
                                      cGAAGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCa                                                                                                           <  2:1273117/150‑1 (MQ=255)
                                       gAAGGTGTCGTCGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGACGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCATCGTTTATGGTAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAg                                                                                                          <  1:2363172/150‑1 (MQ=255)
                                         aGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGt                                                                                                        >  2:1920655/1‑150 (MQ=255)
                                             gtCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGTCGCCCAGATGCAGATCGCCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTGGAAGGTCagg                                                                                                    <  1:2119115/150‑3 (MQ=255)
                                             gtCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCacg                                                                                                    <  2:2212556/150‑3 (MQ=255)
                                              tCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGACAGCGTTGTGTGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAgg                                                                                                   >  2:65488/1‑150 (MQ=255)
                                                gacgaGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGtt                                                                                                 >  2:1827854/1‑150 (MQ=255)
                                                        ggAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAAcgt                                                                                         <  2:543153/150‑1 (MQ=255)
                                                         gAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAAcgtg                                                                                        >  1:1571510/1‑150 (MQ=255)
                                                             tGGGCACGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGcc                                                                                                                               >  2:357456/1‑107 (MQ=255)
                                                             tGGGCACGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGcc                                                                                                                               <  1:357456/107‑1 (MQ=255)
                                                              gggCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGa                                                                                   >  2:954508/1‑150 (MQ=255)
                                                               ggCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAacca                                                                                                                                         >  1:2396930/1‑95 (MQ=255)
                                                               ggCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTCATGGCAacca                                                                                                                                         <  2:2396930/95‑1 (MQ=255)
                                                                 cATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGc                                                                                <  1:1920655/150‑1 (MQ=255)
                                                                   tGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATgg                                                                                                                                               >  1:2367975/1‑85 (MQ=255)
                                                                   tGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATgg                                                                                                                                               <  2:2367975/85‑1 (MQ=255)
                                                                   tGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCa                                                                              <  2:1571510/150‑1 (MQ=255)
                                                                    gCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAgc                                                                                                                        <  1:832415/107‑1 (MQ=255)
                                                                    gCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAgc                                                                                                                        >  2:832415/1‑107 (MQ=255)
                                                                    gCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGAc                                                                                  <  1:2392214/145‑1 (MQ=255)
                                                                    gCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGAGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGAc                                                                                  >  2:2392214/1‑145 (MQ=255)
                                                                     taGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGc                                                                            <  1:1717447/149‑1 (MQ=255)
                                                                     cAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGc                                                                            <  2:2334738/150‑1 (MQ=255)
                                                                           cACGCTTCGGCGCCGGACGGTCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACgaa                                                                      <  1:341130/150‑1 (MQ=255)
                                                                           cACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACgaa                                                                      <  1:954508/150‑1 (MQ=255)
                                                                            aCGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCa                                                                                                           <  2:2252004/112‑1 (MQ=255)
                                                                            aCGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCa                                                                                                           >  1:2252004/1‑112 (MQ=255)
                                                                                ttCGGCGCCGGACGGCGGGCTGCCAGTGGTGACCGCCCGCAACAGCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAAt                                                                 <  2:264454/150‑1 (MQ=255)
                                                                                ttCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAAt                                                                 >  1:264454/1‑150 (MQ=255)
                                                                                      gcCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAaccacc                                                                                                                                       <  1:2257674/74‑1 (MQ=255)
                                                                                      gcCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAaccacc                                                                                                                                       >  2:2257674/1‑74 (MQ=255)
                                                                                        cGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCAcc                                                         <  2:208204/150‑1 (MQ=255)
                                                                                               ccAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCGACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACAt                                                  >  1:2190708/1‑150 (MQ=255)
                                                                                               ccAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCAACGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTTACCGTCACAt                                                  >  1:372501/1‑150 (MQ=255)
                                                                                                cAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATc                                                 <  2:754761/150‑1 (MQ=255)
                                                                                                 aGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTca                                                     <  2:1082347/145‑1 (MQ=255)
                                                                                                 aGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTca                                                     >  1:1082347/1‑145 (MQ=255)
                                                                                                   aTGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCCACTAAGAAATCGCTCACCGTCACATCCAc                                              <  1:65488/150‑1 (MQ=255)
                                                                                                    tGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGCACATGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACg                                             <  1:1162258/150‑1 (MQ=255)
                                                                                                     gCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACgg                                            <  1:2237687/150‑1 (MQ=255)
                                                                                                        gATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCACGGTtgt                                                                                               <  2:1844495/96‑1 (MQ=255)
                                                                                                        gATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTtgt                                                                                               >  1:1844495/1‑96 (MQ=255)
                                                                                                        gATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACgg                                            >  1:1094902/1‑147 (MQ=255)
                                                                                                        gATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACgg                                            <  2:1094902/147‑1 (MQ=255)
                                                                                                        gATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGa                                         >  2:41371/1‑150 (MQ=255)
                                                                                                         aTCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGaa                                        >  2:2357213/1‑150 (MQ=255)
                                                                                                               aCCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGc                                  >  1:1568017/1‑150 (MQ=255)
                                                                                                                ccATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGcc                                 <  1:1827854/150‑1 (MQ=255)
                                                                                                                 cATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGCCa                                >  1:2384027/1‑150 (MQ=255)
                                                                                                                   tCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGCCATc                              >  1:2284835/1‑150 (MQ=255)
                                                                                                                      gcgACATCGACGGCGACGACGTCACCGTTGATGGCAACCACACACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGCCATcacc                           >  1:2359232/1‑150 (MQ=255)
                                                                                                                          cATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGCCATCACCACTg                       >  2:336142/1‑150 (MQ=255)
                                                                                                                          cATCAACGGTGACGTCGTCGCCGTCGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGCCATCACCACTg                       >  2:122243/1‑150 (MQ=255)
                                                                                                                             cGACGGCGACGACGTCACCGTTGATGGCAACCACCTACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGCCATCACCACTGAtt                    <  2:2370970/150‑1 (MQ=255)
                                                                                                                              aaCGGTGACGTCGTCGCCGTCGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACg                                             >  1:1694125/2‑124 (MQ=255)
                                                                                                                              aaCGGTGACGTCGTCGCCGTCGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACg                                             <  2:1694125/123‑1 (MQ=255)
                                                                                                                               aCGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGCCATCACCACTGAtttt                  >  1:458605/1‑150 (MQ=255)
                                                                                                                                 ggCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGCCATCACCACTGATTTTCt                <  1:514497/150‑1 (MQ=255)
                                                                                                                                  gCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGCCATCACCACTGATTTTCTg               <  2:2384027/150‑1 (MQ=255)
                                                                                                                                   cgacgaCGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGCCATCACCACTGATTTTCTgc              <  2:2284835/150‑1 (MQ=255)
                                                                                                                                     acgacgTCACCGTTGATGGCGACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGCCATCACCACTGATTTTCTgcgc            <  2:2190708/150‑1 (MQ=255)
                                                                                                                                          gTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGCCATCACCACTGATTTTCTGCGCTAAGc       >  1:806607/1‑150 (MQ=255)
                                                                                                                                             aCCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCGAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTAAAGGTGACCATCACCACTGATTTTCTGCGCTAAGCTCa    >  1:2340011/1‑150 (MQ=255)
                                                                                                                                             aCCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGCCATCACCACTGATTTTCTGCGCTAAGCTCa    <  1:336142/150‑1 (MQ=255)
                                                                                                                                             aCCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGCCATCACCACTGATTTTCTGCGCTAAGCTCa    <  2:111018/150‑1 (MQ=255)
                                                                                                                                               cGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGCCATCACCACTGATTTTCTGCGCTAAGCTCAGa  >  2:2016608/1‑150 (MQ=255)
                                                                                                                                               cGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTAAAGGTTGTGAACGTGCGTGACGCCAGCGCCGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGCCATCACCACTGATTTTCTGCGCTAAGCTCAGa  >  2:688358/1‑150 (MQ=255)
                                                                                                                                                gTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGCCATcacca                          <  1:2159784/125‑1 (MQ=255)
                                                                                                                                                gTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGCCATcacca                          >  2:2159784/1‑125 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTGGCCGAACTGGTCAGCACCCTGAACCGTAGCCTGTTCGAAGGTGTCGACGAGCTGGAAGTGGGCATGCAGTTCCACGCTTCGGCGCCGGACGGCCAGATGCAGATCGTCACCATCCGCGACATCGACGGCGACGACGTCACCGTTGATGGCAACCACCCACTGGCCGGTCAGCGCCTGACCTTCCAGGTCAAGGTTGTGAACGTGCGTGACGCCAGCGACGAAGAAATCGCTCACCGTCACATCCACGGTGAAGGTGGCCATCACCACTGATTTTCTGCGCTAAGCTCAGA  >  NC_002947/894263‑894555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑