Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,028,002 G→C 6.5% D247E (GAC→GAG)  dppA‑III ← dipeptide ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,028,0020GC 6.5% 275.5 / 6.2 87D247E (GAC→GAG) dppA‑IIIdipeptide ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (38/43);  new base C (2/4);  total (40/47)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTGTAGGCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGC  >  NC_002947/1027854‑1028151
                                                                                                                                                    |                                                                                                                                                     
cGTTGTAGGCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCCCTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCgc                                                                                                                                                      >  2:128920/1‑149 (MQ=255)
 gTTGTAGGCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTc                                                                                                                                                     >  1:1759349/1‑150 (MQ=255)
 gTTGTACGCAAAAGATCCGAGGGTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTc                                                                                                                                                     >  2:1306565/1‑150 (MQ=255)
     tAGGCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCCCCGAGGCGTCggtg                                                                                                                                                 <  2:94538/150‑1 (MQ=255)
     tAGGCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCggtg                                                                                                                                                 <  1:731362/150‑1 (MQ=255)
          gATGTAGCCGAGGTTGAAACCTGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCAGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGAt                                                                                                                                            <  2:1666353/150‑1 (MQ=255)
                  cGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGa                                                                                                                                    >  1:468403/1‑150 (MQ=255)
                     ggTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCa                                                                                                                                 <  1:1356052/150‑1 (MQ=255)
                      gTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAg                                                                                                                                <  1:1158209/150‑1 (MQ=255)
                       ttGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAgg                                                                                                                               >  2:2227943/1‑150 (MQ=255)
                       ttGAAACCGGCCTGCTGCGGCATTTGAAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAgg                                                                                                                               >  1:664466/1‑150 (MQ=255)
                        tGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCaagg                                                                                                                              <  2:1523039/150‑4 (MQ=255)
                           aaCCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCTTGCGCCCCCAGGCGTCCGTGGTCATGGCACAGACCAGATTGt                                                                                                                           <  2:94283/150‑1 (MQ=255)
                              cGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGa                                                                                                                        >  1:1200117/1‑150 (MQ=255)
                              cGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGa                                                                                                                        >  1:124472/1‑150 (MQ=255)
                               ggCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGAt                                                                                                                       <  1:250198/150‑1 (MQ=255)
                                   tgctgcGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTc                                                                                                                   <  2:468403/150‑1 (MQ=255)
                                   ggctgcGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTc                                                                                                                   <  1:1306565/149‑1 (MQ=255)
                                            aTTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCg                                                                                                          <  1:2227943/150‑1 (MQ=255)
                                                   ggTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGACGCCTCggtgc                                                                                                                                                >  1:789541/1‑104 (MQ=255)
                                                   ggTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGACGCCTCggtgc                                                                                                                                                <  2:789541/105‑2 (MQ=255)
                                                    gTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCa                                                                                                  >  1:4648/1‑150 (MQ=255)
                                                    gTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCa                                                                                                  <  1:721324/150‑1 (MQ=255)
                                                    gTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCa                                                                                                  >  1:862267/1‑150 (MQ=255)
                                                    gTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCa                                                                                                  >  1:1090686/1‑150 (MQ=255)
                                                    gTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCa                                                                                                  <  2:1496743/150‑1 (MQ=255)
                                                     ttCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCag                                                                                                 <  1:491080/150‑1 (MQ=255)
                                                       cTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCagta                                                                                               <  2:1766041/150‑1 (MQ=255)
                                                        ttGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCa                                                                                                                                        >  1:979910/1‑108 (MQ=255)
                                                        ttGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCa                                                                                                                                        <  2:979910/108‑1 (MQ=255)
                                                        ttGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCa                                                                                                  >  1:1066475/1‑146 (MQ=255)
                                                        ttGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCa                                                                                                  <  2:1066475/146‑1 (MQ=255)
                                                           tCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTcc                                                                                           >  1:1108057/1‑150 (MQ=255)
                                                           tCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTcc                                                                                           >  1:1204709/1‑150 (MQ=255)
                                                            cGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTccc                                                                                          <  2:1090686/150‑2 (MQ=255)
                                                             ggCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCtt                                                                                         <  2:1200117/150‑1 (MQ=255)
                                                              gCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCttg                                                                                        <  2:862267/150‑1 (MQ=255)
                                                               cTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCttgt                                                                                       >  2:52093/1‑150 (MQ=255)
                                                               cTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCttgt                                                                                       >  2:1070360/1‑150 (MQ=255)
                                                                ttttAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCttgtt                                                                                      >  2:2280973/1‑150 (MQ=255)
                                                                ttttAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCttgtt                                                                                      <  1:1483441/150‑1 (MQ=255)
                                                                ttttAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTATCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCttgtt                                                                                      >  1:2308330/1‑150 (MQ=255)
                                                                 tttAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGAt                                                                                                                       <  2:2032691/116‑1 (MQ=255)
                                                                 tttAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGAt                                                                                                                       >  1:2032691/1‑116 (MQ=255)
                                                                        gCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCgg                                                                                                         >  1:469826/1‑123 (MQ=255)
                                                                        gCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCgg                                                                                                         <  2:469826/123‑1 (MQ=255)
                                                                        gCTCGATGTCGGCCGGGCGTGGGAACAGGGGGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGACCTTGa                                                                              >  2:1209922/1‑150 (MQ=255)
                                                                          tCGATGTCGGCCGGGAGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCagtagt                                                                                             >  1:83436/1‑133 (MQ=255)
                                                                          tCGATGTCGGCCGGGAGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCagtagt                                                                                             <  2:83436/133‑1 (MQ=255)
                                                                           ctaTGTCGGCCGGGCGTGCGAAAACGATGCCCTCGCACTCGTTCTTCTTGAGCTTCTGCATGCGCCCCGCGATCTTGGTGGTTATGGCAAAGATTAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGc                                                                           <  2:2308330/148‑1 (MQ=255)
                                                                           cGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTTGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGc                                                                           <  1:128920/150‑1 (MQ=255)
                                                                            gATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCg                                                                          >  2:2094156/1‑150 (MQ=255)
                                                                              tGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGa                                                                        <  2:664466/150‑1 (MQ=255)
                                                                                   gCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCttc                                                                   <  1:1070360/150‑3 (MQ=255)
                                                                                   gCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTgc                                                                   >  2:1091445/1‑150 (MQ=255)
                                                                                   gCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTgc                                                                   <  2:1204709/150‑1 (MQ=255)
                                                                                         cGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTcc                                                             <  2:1113384/150‑1 (MQ=255)
                                                                                          gTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTgcgc                                                                 <  1:1409560/145‑1 (MQ=255)
                                                                                          gTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTgcgc                                                                 >  2:1409560/1‑145 (MQ=255)
                                                                                              gAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTg                                                        <  1:1091445/150‑1 (MQ=255)
                                                                                                  aGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGaa                                                                             <  1:2359168/125‑1 (MQ=255)
                                                                                                  aGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGaa                                                                             >  2:2359168/1‑125 (MQ=255)
                                                                                                    ggTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTAttt                                                  <  1:2280973/150‑1 (MQ=255)
                                                                                                          cTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCg                                                                                                          >  2:1055389/1‑88 (MQ=255)
                                                                                                          cTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCg                                                                                                          <  1:1055389/88‑1 (MQ=255)
                                                                                                          cTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGTGGATCTGCGCGTCCTTCTGGTATTTGCTGaa                                            >  2:1204564/1‑150 (MQ=255)
                                                                                                          cTGGCACTCGTTCTTATTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGCCCCCAAAGATCAGGTTCTCGATCTTCACGTCCTCGGGCTGCCAGTCCTCCTTGTTGCCCCTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGaa                                            >  1:431670/1‑150 (MQ=255)
                                                                                                               aCTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTgg                                                       >  2:755492/1‑134 (MQ=255)
                                                                                                               aCTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTgg                                                       <  1:755492/134‑1 (MQ=255)
                                                                                                                   gTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGcc                                                                                   >  1:2244365/1‑102 (MQ=255)
                                                                                                                   gTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGcc                                                                                   <  2:2244365/102‑1 (MQ=255)
                                                                                                                   gTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACgg                                   <  2:1759349/150‑1 (MQ=255)
                                                                                                                           tGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGc                           <  1:52093/150‑1 (MQ=255)
                                                                                                                              gCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGa                        >  2:1266485/1‑150 (MQ=255)
                                                                                                                                ttCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATc                      >  2:1037421/1‑150 (MQ=255)
                                                                                                                                ttCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATc                      <  1:1037421/150‑1 (MQ=255)
                                                                                                                                   tGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGc                   <  1:1266485/150‑1 (MQ=255)
                                                                                                                                     catcaccACCGACGCCTGGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGc                   >  2:1237515/7‑148 (MQ=39)
                                                                                                                                     catcaccACCGACGCCTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGc                   <  1:1237515/142‑1 (MQ=255)
                                                                                                                                     cATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCCGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCtt                 >  2:1798446/1‑150 (MQ=255)
                                                                                                                                          gcACCGAGGCGTCGGTGTTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGt            >  2:852302/1‑150 (MQ=255)
                                                                                                                                          gcACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGt            >  2:268545/1‑150 (MQ=255)
                                                                                                                                           cgccGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGCACACGAACGGGCCGGTGCCGATCGGCTTCTGGtt           <  1:2021294/148‑1 (MQ=255)
                                                                                                                                           cACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGTGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGtt           <  1:1204564/150‑1 (MQ=255)
                                                                                                                                           cACCGACGCCTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGtt           <  2:1294313/150‑1 (MQ=255)
                                                                                                                                            aCCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCTGTGCCGATCGGCTTCTGGTTg          >  2:374282/1‑150 (MQ=255)
                                                                                                                                            aCCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTg          >  2:1890314/1‑150 (MQ=255)
                                                                                                                                              cGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGAt        <  2:124472/150‑1 (MQ=255)
                                                                                                                                                    gTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTcggc  >  1:1076171/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGTTGTAGGCGATATAGCCGAGGTTGAAACCGGCCTGCTGCGGCATTTGCAGGTTCTTGTCGGCTTTTAGCGGCTCGATGTCGGCCGGGCGTGGGAACAGGGTGACCTGGCACTCGTTCTTCTTGAGCTTCTGCATGCGCACCGAGGCGTCGGTGGTGATGGCAAAGATCAGGTTGTCGATCTTCACGTCCTCGGGCTGCCAGTAGTCCTTGTTGCCCTTGAAGCGGATCTGCGCGTCCTTCTGGTATTTGCTGAACACGAACGGGCCGGTGCCGATCGGCTTCTGGTTGATGTCGGC  >  NC_002947/1027854‑1028151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑