Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 129,654 T→A 5.7% L711Q (CTG→CAG)  polA → DNA polymerase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947129,6540TA 5.7% 276.6 / 5.6 88L711Q (CTG→CAG) polADNA polymerase I
Reads supporting (aligned to +/- strand):  ref base T (48/35);  new base A (3/2);  total (51/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

GAACTTGCAGAACATCCCGATCCGCACCGCTGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGCCTGATCTACGGCATGAGCGCCTTTGGCCTGG  >  NC_002947/129505‑129803
                                                                                                                                                     |                                                                                                                                                     
gAACTTGCAGAACATCCCGATCCGCACCGCTGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCTTGGCCCACCTGGCCAAGGACGAAGGCTtgct                                                                                                                                                       <  1:1667108/150‑1 (MQ=255)
    ttGCAGAACATCCCGATCCGCACCGCTGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCAt                                                                                                                                                   <  2:1812283/150‑1 (MQ=255)
    ttGCAGAACATCCCGATCCGCACCGCTGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCAt                                                                                                                                                   >  1:2385210/1‑150 (MQ=255)
    ttGCAGAACATCCCGATCCGCACCGCTGAAGGGCGGCGTATCCGCCAGCAGTTCAACGCCAGTACGGGTTACAATCTTCTGGAGGCGGACTATTCAAAGATCGAGCTGCGTATCATCGCCCACCTGGACAAGGACGAAGGCTTTCTGCAt                                                                                                                                                   >  2:179285/1‑150 (MQ=255)
        aGAACATCCCGATCCGCACCGCTGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCt                                                                                                                                               >  2:1117697/1‑150 (MQ=255)
        aGAACATCCCGATCCGCACCGCTGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCt                                                                                                                                               <  2:418663/150‑1 (MQ=255)
            cATCCCGATCCGCACCGCTGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCt                                                                                                                                               >  1:1435552/1‑146 (MQ=255)
            cATCCCGATCCGCACCGCTGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCt                                                                                                                                               <  2:1435552/146‑1 (MQ=255)
            cATCCCGATCCGCACCGCTGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCg                                                                                                                                           <  1:1721749/150‑1 (MQ=255)
             aTCCCGATCCGCACCGCTGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGc                                                                                                                                          >  1:2219861/1‑150 (MQ=255)
                ccGCTCCGCACCGCTGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTGCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAAt                                                                                                                                       >  2:1730610/1‑150 (MQ=255)
                     ccGCACCGCTGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCt                                                                                                                                  <  2:2045326/150‑1 (MQ=255)
                     ccGCACCGCTGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCt                                                                                                                                  <  1:1899202/150‑1 (MQ=255)
                      cGCACCGCTGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTg                                                                                                                                 <  2:2219861/150‑1 (MQ=255)
                      cGCACCGCTGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTg                                                                                                                                 >  1:110109/1‑150 (MQ=255)
                       gCACCGCTGAAGGGCGGCGTCTCAGCCAGGCGTTCCTCGCCATGCCGGCTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTgg                                                                                                                                <  2:1832621/150‑1 (MQ=255)
                       gCACCGCTGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCATGCAGCAAGCCTTcg                                                                                                                                            >  1:127317/1‑137 (MQ=255)
                       gCACCGCTGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCATGCAGCAAGCCTTcg                                                                                                                                            <  2:127317/138‑2 (MQ=255)
                       gCACCGCTGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTACGGGTTACAAGCTTATGGCGGAGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTgg                                                                                                                                >  2:255924/1‑150 (MQ=255)
                            gCTGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCAt                                                                                                                                                   <  1:912886/126‑1 (MQ=255)
                            gCTGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCAt                                                                                                                                                   >  2:912886/1‑126 (MQ=255)
                              tGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCa                                                                                                                         <  1:137975/150‑1 (MQ=255)
                                aaGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCAcc                                                                                                                       >  2:1610575/1‑150 (MQ=255)
                                  gggCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGACAAGCACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACcgc                                                                                                                     >  1:163813/1‑150 (MQ=255)
                                       gcgTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTg                                                                                                                           >  2:1735291/1‑139 (MQ=255)
                                       gcgTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTg                                                                                                                           <  1:1735291/139‑1 (MQ=255)
                                       gcgTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCcac                                                                                                                >  1:1551770/1‑150 (MQ=255)
                                           aTCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCAcagca                                                                                                            <  2:110109/150‑1 (MQ=255)
                                               gCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCg                                                                                                        >  1:1874535/1‑150 (MQ=255)
                                                 cAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGCCGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGaa                                                                                                      <  1:1148245/150‑1 (MQ=255)
                                                 cAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGaa                                                                                                      <  2:1874535/150‑1 (MQ=255)
                                                              tgaGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTgg                                                                                         >  1:1892540/3‑150 (MQ=255)
                                                              ccAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTgg                                                                                         <  2:1551770/150‑1 (MQ=255)
                                                                    cGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTgg                                                                                   >  2:206212/1‑150 (MQ=255)
                                                                       gTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAg                                                                                >  2:1313849/1‑150 (MQ=255)
                                                                         tACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACTTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGAc                                                                              <  2:935507/150‑1 (MQ=255)
                                                                         tACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGAc                                                                              <  2:2385210/150‑1 (MQ=255)
                                                                           cAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGt                                                                            <  2:1892540/150‑1 (MQ=255)
                                                                             atcTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTGCGCAATTACCGGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGa                                                                          <  2:19717/148‑1 (MQ=255)
                                                                              gCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAATGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCCCGCCTCAGCAGCCGAAGTGCTCGGTGTGGCCCTGGACGACGTGac                                                                         >  2:875208/1‑150 (MQ=255)
                                                                                ttCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAAt                                                                                                                                       <  2:1783864/86‑1 (MQ=255)
                                                                                ttCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAAt                                                                                                                                       >  1:1783864/1‑86 (MQ=255)
                                                                                ttCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACagcagc                                                                                                          <  1:509505/115‑1 (MQ=255)
                                                                                ttCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACagcagc                                                                                                          >  2:509505/1‑115 (MQ=255)
                                                                                ttCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGcc                                                                                       <  1:797334/134‑1 (MQ=255)
                                                                                ttCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGcc                                                                                       >  2:797334/1‑134 (MQ=255)
                                                                                ttCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGacca                                                                       >  1:1858031/1‑150 (MQ=255)
                                                                                 tCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGaccac                                                                      <  1:446303/150‑1 (MQ=255)
                                                                                     gcggcgGACTTTTCACATATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACTAAACAATCAAATCAGCCTTCCGCAATGACCTTTATGTGCCACGAGCCATAGCAGCGGAAGTGTGCGGTGTGGCCCTGGAAGACGTGACCACCGAc                                                                  <  1:1151081/150‑1 (MQ=255)
                                                                                          ggACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACagcagc                                                                                                          <  1:654826/105‑1 (MQ=255)
                                                                                          ggACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACagcagc                                                                                                          >  2:654826/1‑105 (MQ=255)
                                                                                             cTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGaccac                                                                      >  1:921445/1‑138 (MQ=255)
                                                                                             cTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGaccac                                                                      <  2:921445/138‑1 (MQ=255)
                                                                                                   acaGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGTAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAgcgc                                                    >  2:2387824/1‑150 (MQ=255)
                                                                                                   acaGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAgcgc                                                    >  2:464219/1‑150 (MQ=255)
                                                                                                   acaGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAgcgc                                                    >  2:2357211/1‑150 (MQ=255)
                                                                                                   acaGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTAATTATGGAAGACGTGACCACCGACCAGCGCCGCAgcgc                                                    >  2:194995/1‑150 (MQ=255)
                                                                                                   acaGATCGAGCTGCGTATCATGGCCAACCTGGCCAAGGACGAAGGCTTGCTGGATGCCTTCCGCAATGACCTGGACGTGCACAGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGCAAGACGTGACCACCGACCAGCGCCGCAgcgc                                                    >  2:130001/1‑150 (MQ=255)
                                                                                                       aTCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAg                                                <  2:379784/150‑1 (MQ=255)
                                                                                                       aTCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAg                                                >  2:2190138/1‑150 (MQ=255)
                                                                                                       aTCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGACCTGGAAGATGTGACAACCGACCAGCGCCGCAGCGCCAAg                                                >  1:114647/1‑150 (MQ=255)
                                                                                                        tCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGGTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGGGTTCGGTGTGGCCCTGGAAGACGTGACTACCGACCAGCGCCGCAGCGCCAAgg                                               >  1:2294994/1‑150 (MQ=255)
                                                                                                        tCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAgg                                               <  1:155975/150‑1 (MQ=255)
                                                                                                               gCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCaa                                        >  1:2070867/1‑150 (MQ=255)
                                                                                                                  tATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACtt                                     <  2:49860/150‑1 (MQ=255)
                                                                                                                    tCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCg                                   >  1:954904/1‑150 (MQ=255)
                                                                                                                    tCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCGGGAAGACGTGACCACCGACACGCGCCGCGGCGGCAAGGCCATCAACTTCg                                   >  1:735955/1‑150 (MQ=255)
                                                                                                                     cATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCgg                                  <  1:1117697/150‑1 (MQ=255)
                                                                                                                      aTGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAgc                                                      <  1:534785/129‑1 (MQ=255)
                                                                                                                      aTGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGc                                 >  1:1334187/1‑150 (MQ=255)
                                                                                                                      aTGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGc                                 >  2:562470/1‑150 (MQ=255)
                                                                                                                      aTGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGCGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAgc                                                      >  2:534785/1‑129 (MQ=255)
                                                                                                                        ggCCCACCTGGCCAAGGACGAAGGGTTGCTGCATGCCTTCCGCAATGACGTGGGCGTGCACCGCGCCACGGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGCCt                               >  1:301795/1‑150 (MQ=255)
                                                                                                                         gCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGGAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGCCTg                              >  1:179233/1‑150 (MQ=255)
                                                                                                                         gCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCa                                                        <  1:821985/124‑1 (MQ=255)
                                                                                                                         gCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCa                                                        >  2:821985/1‑124 (MQ=255)
                                                                                                                         gCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGCCTg                              >  2:1326211/1‑150 (MQ=255)
                                                                                                                         gCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGCCTg                              >  2:840796/1‑150 (MQ=255)
                                                                                                                           ccACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGCCTGAt                            >  2:663694/1‑150 (MQ=255)
                                                                                                                                tGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGCCTGATCTACg                       <  2:1302773/150‑1 (MQ=255)
                                                                                                                                    cAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGCCTGATCTACGGCAt                   >  2:1742082/1‑150 (MQ=255)
                                                                                                                                    cAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGCCTGATCTACGGCAt                   >  1:1746251/1‑150 (MQ=255)
                                                                                                                                      aGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGCCTGATCTACGGCATGa                 <  1:562470/150‑1 (MQ=255)
                                                                                                                                       ggACGAAGGCTTGCTGCATCCGTTCCGCAATGACATGGACGTGCACCACGCAACGGCAGCCGAAGTACTCTGGGTGGCCCTGGAAGACGTGACAACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGCCTGATCTACCACATGAg                >  2:377092/1‑150 (MQ=255)
                                                                                                                                        gACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGCCTGATCTACGGCATGAgc               >  2:2032456/1‑150 (MQ=255)
                                                                                                                                         cggAAGGCATGCAGCAAGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGCCTGATCTACGGCATGcgcg              >  2:893240/3‑150 (MQ=255)
                                                                                                                                           gAAGGCATGCAGCAAGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGCCTGATCTACGGCATGAGCGcc            >  2:2163964/1‑150 (MQ=255)
                                                                                                                                            aaGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGCCTGATCTACGGCATGAGCGCCt           <  1:1313849/150‑1 (MQ=255)
                                                                                                                                              ggCTTGCTGCATGCCTTCCGCAATTACCTCGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGCCTGATCTACGGCATGAGCGCCttt         <  1:959436/150‑1 (MQ=255)
                                                                                                                                                    ctgcATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGCCTGATCTACGGCATGAGCGCCTTtggcctg   <  1:179285/150‑1 (MQ=255)
                                                                                                                                                    ctgcATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGCCTGATCTACGGCATGAGCGCCTTtggcctg   <  1:840796/150‑1 (MQ=255)
                                                                                                                                                     tgcATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGCCTGATCTACGGCATGAGCGCCTTtggcctgg  >  1:1711153/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAACTTGCAGAACATCCCGATCCGCACCGCTGAAGGGCGGCGTATCCGCCAGGCGTTCATCGCCAGTCCGGGTTACAAGCTTCTGGCGGCGGACTATTCACAGATCGAGCTGCGTATCATGGCCCACCTGGCCAAGGACGAAGGCTTGCTGCATGCCTTCCGCAATGACCTGGACGTGCACCGCGCCACAGCAGCCGAAGTGTTCGGTGTGGCCCTGGAAGACGTGACCACCGACCAGCGCCGCAGCGCCAAGGCCATCAACTTCGGCCTGATCTACGGCATGAGCGCCTTTGGCCTGG  >  NC_002947/129505‑129803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑