BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,339	T→A	13.5%	Q15L (CAG→CTG)	trmE ←	GTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,339	0	T	A	13.5%	285.1 / 32.9	104	Q15L (CAG→CTG)	trmE	GTPase
Reads supporting (aligned to +/- strand): ref base T (39/50); new base A (6/8); total (46/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

Only 100 of 104 total aligned reads displayed.

CTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCA  >  NC_002947/6196‑6480

cTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCg                                                                                                                                          >  2:680824/1‑150 (MQ=255)

cTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCg                                                                                                                                          >  2:1152906/1‑150 (MQ=255)

cTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCAGGGCCg                                                                                                                                          >  1:933984/1‑150 (MQ=255)

cTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGCACCGTACTGGGGCTG‑GACGGGGTGCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCAGGGCCg                                                                                                                                          >  1:656957/1‑150 (MQ=255)

  tCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTg                                                                                                                                        <  2:1796217/150‑1 (MQ=255)

     tCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCAGGGCCGGGGCg                                                                                                                                     <  1:1056267/150‑1 (MQ=255)

     tCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCAGGGCCGGGGCg                                                                                                                                     >  2:2400631/1‑150 (MQ=255)

      ccAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGa                                                                                                                                    <  1:1671822/150‑1 (MQ=255)

      ccAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCAGGGCCGGGGCgg                                                                                                                                    <  2:656957/150‑2 (MQ=255)

      ccAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCAGGGCCGGGGCgg                                                                                                                                    <  2:1181163/150‑2 (MQ=255)

       cAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGc                                                                                                                                      <  2:715806/147‑1 (MQ=255)

       cAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGc                                                                                                                                      >  1:715806/1‑147 (MQ=255)

        aGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATg                                                                                                                                  >  2:910042/1‑150 (MQ=255)

         gCACCAGCCCTTCATCGTCGCGGAATGGACCGTACTAGTTAGGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATgg                                                                                                                                 <  1:126285/150‑1 (MQ=255)

         gCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACATCGCCACGGCCCAGGGCCGGGGCGGTgt                                                                                                                                 <  2:2173014/150‑2 (MQ=255)

         gCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCAGGGCCGGGGCGGTgt                                                                                                                                 <  1:2400631/150‑2 (MQ=255)

              aGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGgcggcg                                                                                                                            >  2:1772496/1‑150 (MQ=255)

               gCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGAGT‑CCGGGTGCAAGCAGTAATTAACTTGCCTGCCTTGCCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCTTGGCGATGGCGGCGa                                                                                                                           <  1:1489147/150‑1 (MQ=255)

                cccTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGAt                                                                                                                          <  2:1213976/150‑1 (MQ=255)

                cccTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGAt                                                                                                                          <  1:680824/150‑1 (MQ=255)

                 ccTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATg                                                                                                                         <  1:1603403/150‑1 (MQ=255)

                       tcgtcgCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCa                                                                                                                   <  1:787875/150‑1 (MQ=255)

                        cgtcgCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCAc                                                                                                                  <  2:1990049/150‑1 (MQ=255)

                        cgtcgCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCAc                                                                                                                  <  1:388374/150‑1 (MQ=255)

                          tcgCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGTACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGttt                                                                                                                     >  2:804586/1‑145 (MQ=255)

                          tcgCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGttt                                                                                                                     <  1:804586/145‑1 (MQ=255)

                               gAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAgt                                                                                                           <  1:931644/150‑1 (MQ=255)

                               gAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAgt                                                                                                           >  1:89244/1‑150 (MQ=255)

                                    gACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATgg                                                                                                                                 >  2:1068359/1‑123 (MQ=255)

                                    gACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATgg                                                                                                                                 <  1:1068359/123‑1 (MQ=255)

                                        gTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCt                                                                                                  <  2:2303698/150‑1 (MQ=255)

                                           aTGGGCATGCCGCGGGCT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGa                                                                                               >  1:83667/1‑150 (MQ=255)

                                            tGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCAGGGCCGGGGCggtgt                                                                                                                                 >  2:867578/1‑111 (MQ=255)

                                            tGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCAGGGCCGGGGCgg                                                                                                                                    >  1:457322/1‑111 (MQ=255)

                                            tGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCAGGGCCGGGGCgg                                                                                                                                    <  2:457322/112‑2 (MQ=255)

                                             gggCACGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                             <  1:757394/150‑1 (MQ=255)

                                                cATGCCGCGGGGT‑CAGGGGGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGcc                                                                                          <  2:89244/150‑1 (MQ=255)

                                                 aTGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCct                                                                                         >  1:614000/1‑150 (MQ=255)

                                                     cgcgGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGTACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTAc                                                                                     <  1:1969789/150‑1 (MQ=255)

                                                        ggggT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTCTTCATGCTCGAAAGCCTCTACGAc                                                                                  <  1:140956/150‑1 (MQ=255)

                                                            t‑CAGGGTGCGGCCGGTGATAAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTTAAAATTCCCACACCGGCCCGGCTCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACcaaa                                                                              >  2:579203/1‑150 (MQ=255)

                                                                  gTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACGGCCCCGGCCCGGGGCCGTGGCGATGGCGGCGCTGGTTTCACGCACGGTGTTCATGCTCGAAAGCCTCTACGCCAAAAACGAc                                                                         >  2:691486/1‑150 (MQ=255)

                                                                   tGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACa                                                                        <  1:654107/150‑1 (MQ=255)

                                                                   tGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACa                                                                        <  2:1067211/150‑1 (MQ=255)

                                                                   tGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACa                                                                        <  1:1772496/150‑1 (MQ=255)

                                                                      ggCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGAt                                                                     >  2:1034602/1‑150 (MQ=255)

                                                                      ggCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGAt                                                                     <  2:1397018/150‑1 (MQ=255)

                                                                       gCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATa                                                                    >  1:2015501/1‑150 (MQ=255)

                                                                                 aaCAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGc                                                          >  2:477136/1‑150 (MQ=255)

                                                                                  acgggccggccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGcc                                                         >  1:2409144/4‑150 (MQ=255)

                                                                                  aCAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAAc                                                            <  2:703099/147‑1 (MQ=255)

                                                                                  aCAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAAc                                                            >  1:703099/1‑147 (MQ=255)

                                                                                  aCAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGcc                                                         >  2:1066429/1‑150 (MQ=255)

                                                                                           ccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAg‑gg                                               <  1:1104685/150‑1 (MQ=255)

                                                                                               ggCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTc                                                                                                                    >  1:2301029/1‑78 (MQ=255)

                                                                                               ggCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTc                                                                                                                    <  2:2301029/78‑1 (MQ=255)

                                                                                                gCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGt                                          >  2:1581793/1‑150 (MQ=255)

                                                                                                  aGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCC‑CTAGTGGGGCGttt                                        <  2:1758710/150‑1 (MQ=255)

                                                                                                    ccAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggcg                                                                                                                               <  1:2051464/62‑1 (MQ=255)

                                                                                                    ccAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggcg                                                                                                                               >  2:2051464/1‑62 (MQ=255)

                                                                                                    ccAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGttttt                                      >  2:248724/1‑150 (MQ=255)

                                                                                                         gggCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTc                                 <  1:931821/150‑1 (MQ=255)

                                                                                                            ccGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGc                                                          >  2:817269/1‑123 (MQ=255)

                                                                                                            ccGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGc                                                          <  1:817269/123‑1 (MQ=255)

                                                                                                             cGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGTTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTa                                    <  1:1011667/143‑1 (MQ=255)

                                                                                                             cGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTa                                    >  2:1011667/1‑143 (MQ=255)

                                                                                                                 cAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACCGGCTa                         <  1:1152906/150‑1 (MQ=255)

                                                                                                                    ccTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCg                      <  2:1352018/150‑1 (MQ=255)

                                                                                                                    ccTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCg                      >  1:2335073/1‑150 (MQ=255)

                                                                                                                     cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGt                     >  1:1815489/1‑150 (MQ=255)

                                                                                                                     cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGt                     >  2:1805098/1‑150 (MQ=255)

                                                                                                                      tGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGTTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGc                       >  2:839234/1‑147 (MQ=255)

                                                                                                                      tGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGTTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGc                       <  1:839234/147‑1 (MQ=255)

                                                                                                                      tGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAg                                                  >  2:1811501/1‑121 (MQ=255)

                                                                                                                      tGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAg                                                  <  1:1811501/121‑1 (MQ=255)

                                                                                                                      tGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTa                    >  1:629505/1‑150 (MQ=255)

                                                                                                                        aCAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTAtt                  >  1:825507/1‑150 (MQ=255)

                                                                                                                           aTTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                             >  1:1430115/1‑73 (MQ=255)

                                                                                                                           aTTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                             <  2:1430115/73‑1 (MQ=255)

                                                                                                                           aTTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGc               <  1:1337715/150‑1 (MQ=255)

                                                                                                                           aTTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGc               <  1:1248369/150‑1 (MQ=255)

                                                                                                                           aTTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGc               >  1:1574240/1‑150 (MQ=255)

                                                                                                                            ttCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGcc              <  1:1034602/150‑1 (MQ=255)

                                                                                                                             tCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGccc             <  2:2409144/150‑1 (MQ=255)

                                                                                                                             tCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGccc             <  2:614000/150‑1 (MQ=255)

                                                                                                                             tCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGccc             <  2:629505/150‑1 (MQ=255)

                                                                                                                             tCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGccc             <  1:1581793/150‑1 (MQ=255)

                                                                                                                             ccgatctccGCCCCGGACCTGGGCCGTGGCGATGAGGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGGCAAAATCGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTGTTTATCCACAGGCTAGCGTATTGGccc             <  2:360608/143‑1 (MQ=255)

                                                                                                                              cccACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATTCTTGCAGGGGGCCCGTTG‑GACGGGTTTGTGATTCACAGGCTAGCGTATTAGCCCg            <  1:910042/150‑1 (MQ=255)

                                                                                                                               ccACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGcc                                                         <  1:2072297/105‑1 (MQ=255)

                                                                                                                               ccACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGcc                                                         >  2:2072297/1‑105 (MQ=255)

                                                                                                                               ccACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGt           >  1:1839927/1‑150 (MQ=255)

                                                                                                                                cacaCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTc          >  1:819473/1‑150 (MQ=255)

                                                                                                                                  cacCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCaa        <  1:477136/150‑1 (MQ=255)

                                                                                                                                     cGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCGCTAG‑GGGGCGGTTTTTATTCACAGGCTAGCGTATTAGCCCgagatcgg     >  1:360608/1‑143 (MQ=255)

                                                                                                                                      gCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGAc                                                                         >  1:833823/1‑82 (MQ=255)

                                                                                                                                      gCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGAc                                                                         <  2:833823/82‑1 (MQ=255)

                                                                                                                                       ccccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcagc   >  1:1197345/1‑150 (MQ=255)

                                                                                                                                       ccacGGCCCAGGGCCGGGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcagc   >  1:290667/4‑150 (MQ=255)

                                                                                                                                        cccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcagca  <  2:2335073/150‑1 (MQ=255)

CTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGT‑CAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAG‑GGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCA  >  NC_002947/6196‑6480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`