Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,198,623 C→T 6.5% D309D (GAC→GAT) ‡ gspE → protein secretion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,198,6230CT 6.5% 310.0 / 8.4 107D309D (GAC→GAT) ‡gspEprotein secretion protein
Reads supporting (aligned to +/- strand):  ref base C (48/52);  new base T (3/4);  total (51/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

Only 100 of 109 total aligned reads displayed.

GCCTGAACAGCCTCAACGATGGCAGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGCGGTAGGCGCAGTGACCCGCCTGCGCGACATGGGCATCGA  >  NC_002947/1198475‑1198772
                                                                                                                                                     |                                                                                                                                                     
gCCTGAACAGCCTCAACGATGGCAGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGAcc                                                                                                                                                      >  2:4205/1‑150 (MQ=255)
       cAGCCTCAACGATGGCAGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGGTCCTGACgc                                                                                                                                               <  2:2249504/150‑2 (MQ=255)
       cAGCCTCAACGATGGCAGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGt                                                                                                                                               <  1:2377443/150‑1 (MQ=255)
       cAGCCTCAACGATGGCAGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGt                                                                                                                                               >  2:2377443/1‑150 (MQ=255)
       cAGCCTCAACGATGGCAGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCCGCGCGCCAT‑CCTGCGTCAGGACCCTGACGt                                                                                                                                               >  2:1101560/1‑150 (MQ=255)
        aGCCTCAACGATGGCAGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGGTCCTGACgca                                                                                                                                              <  1:160173/150‑3 (MQ=255)
         gCCTCAACGATGGCAGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGCCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTgt                                                                                                                                             <  1:1044423/150‑2 (MQ=255)
           cTCAACGATGGCAGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCTGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATc                                                                                                                                           >  2:1742897/1‑150 (MQ=255)
           cTCAACGATGGCAGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATc                                                                                                                                           >  1:1532392/1‑150 (MQ=255)
            tCAACGATGGCAGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGAc                                                                                                                                                 >  2:847794/1‑143 (MQ=255)
            tCAACGATGGCAGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGAc                                                                                                                                                 <  1:847794/143‑1 (MQ=255)
            tCAACAATGGCAGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCa                                                                                                                                          >  1:2058739/1‑150 (MQ=255)
              aaCGATGGCAGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGa                                                                                                                                             >  1:2004816/1‑145 (MQ=255)
                   tGGCAGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATc                                                                                                                                           >  2:10403/1‑142 (MQ=255)
                   tGGCAGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATc                                                                                                                                           <  1:10403/142‑1 (MQ=255)
                    ggCAGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGc                                                                                                                                  <  2:1794770/150‑1 (MQ=255)
                    ggCAGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGc                                                                                                                                  <  1:1236198/150‑1 (MQ=255)
                    ggCAGCAGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGAACCCTGACGTGATCATGCTCGGc                                                                                                                                  >  1:59429/1‑150 (MQ=255)
                      cAGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGGTCCTGACgcagga                                                                                                                                           >  2:1040042/1‑134 (MQ=255)
                      cAGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGGTCCTGACgcagga                                                                                                                                           <  1:1040042/139‑6 (MQ=255)
                       aGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGaa                                                                                                                               >  2:2245376/1‑150 (MQ=255)
                         ccGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAAt                                                                                                                             >  2:1816338/1‑150 (MQ=255)
                         ccGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGCCCCGGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAAt                                                                                                                             <  1:148896/150‑1 (MQ=255)
                            cAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCCGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCcg                                                                                                                          >  2:974336/1‑150 (MQ=255)
                             aaCATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCcgc                                                                                                                         >  2:53663/1‑150 (MQ=255)
                             aaCATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCAAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCcgc                                                                                                                         <  1:1343193/150‑1 (MQ=255)
                              aCATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGACGGGAAGCAGTCCGCCAGTGTCC‑CCTTGCGTTGTGTTTGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCcgcg                                                                                                                        <  1:45000/150‑1 (MQ=255)
                               cATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGa                                                                                                                       >  2:1953097/1‑150 (MQ=255)
                                aTCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGAt                                                                                                                      <  1:2002831/150‑1 (MQ=255)
                                 tCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATc                                                                                                                     <  1:1460120/150‑1 (MQ=255)
                                 tCCTCACCGTCGAGGACCCGGTGGAGTACGCCAATGACGGCATGGCCCATACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATc                                                                                                                     <  1:1738176/150‑1 (MQ=255)
                                   cTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAg                                                                                                                   <  2:1851269/150‑1 (MQ=255)
                                   cTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAg                                                                                                                   <  2:1851348/150‑1 (MQ=255)
                                    tCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAgg                                                                                                                  >  2:239737/1‑150 (MQ=255)
                                       ccGTCGAGGACCCGTGGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGgaga                                                                                                               <  1:495801/150‑1 (MQ=255)
                                       ccGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGgaga                                                                                                               <  1:2400138/150‑1 (MQ=255)
                                              ggACCCGGTGGAGTACGCCATCTCCGGCATTGGCCAAACCGCCATCAACCCGCCTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACCTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCa                                                                                                        <  1:2301007/150‑1 (MQ=255)
                                              ggACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCa                                                                                                        <  1:1300424/150‑1 (MQ=255)
                                               gACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCTTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAg                                                                                                       >  1:522426/1‑150 (MQ=255)
                                                  ccGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGGGTAAATACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATc                                                                                                    >  1:636370/1‑150 (MQ=255)
                                                  ccGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATc                                                                                                    >  1:2192891/1‑150 (MQ=255)
                                                     gTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGcc                                                                                                 >  2:766837/1‑150 (MQ=255)
                                                           tACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAg                                                                                           <  1:987919/150‑1 (MQ=255)
                                                            aCGCAATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAgg                                                                                          <  1:1074839/150‑1 (MQ=255)
                                                               ccATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAg                                                                                           <  2:2117414/146‑1 (MQ=255)
                                                                 aTCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCa                                                                                                        <  1:19631/131‑1 (MQ=255)
                                                                 aTCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCa                                                                                                        >  2:19631/1‑131 (MQ=255)
                                                                 aTCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGc                                                                                     >  1:1487081/1‑150 (MQ=255)
                                                                     ccGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAg                                                                                           <  2:1859671/140‑1 (MQ=255)
                                                                     ccGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAg                                                                                           >  1:1859671/1‑140 (MQ=255)
                                                                     ccGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCa                                                                                 >  2:2397632/1‑150 (MQ=255)
                                                                       gacATTGGCCAAACCGTCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCAcc                                                                               <  1:1613333/148‑1 (MQ=255)
                                                                          aTTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCCCCGTGCAGGCCAGCCTCACCGGc                                                                            >  1:1495609/1‑150 (MQ=255)
                                                                             ggCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGcc                                                                                    >  2:1902738/1‑139 (MQ=255)
                                                                             ggCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGcc                                                                                    <  1:1902738/139‑1 (MQ=255)
                                                                             ggCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCAc                                                                         <  1:4205/150‑1 (MQ=255)
                                                                              gCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCAcc                                                                        >  2:585304/1‑150 (MQ=255)
                                                                                      gCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGc                                                                                     <  1:2038520/129‑1 (MQ=255)
                                                                                      gCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGc                                                                                     >  2:2038520/1‑129 (MQ=255)
                                                                                         aTCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCg                                                             >  1:1461385/1‑150 (MQ=255)
                                                                                          tCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGa                                                            >  2:1001728/1‑150 (MQ=255)
                                                                                              cccGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCtt                                                        >  2:1436165/1‑150 (MQ=255)
                                                                                               ccGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTg                                                                   <  2:970135/138‑1 (MQ=255)
                                                                                               ccGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTg                                                       <  1:320888/150‑1 (MQ=255)
                                                                                                cgcgTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGc                                                      <  1:1292080/150‑1 (MQ=255)
                                                                                                cgcgTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGc                                                      <  1:239737/150‑1 (MQ=255)
                                                                                                    tGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCacac                                                  <  1:1001728/150‑1 (MQ=255)
                                                                                                       cGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACcaa                                               <  2:1487081/150‑1 (MQ=255)
                                                                                                       cGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACcaa                                               >  1:1475698/1‑150 (MQ=255)
                                                                                                       cGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACcaa                                               <  2:432332/150‑1 (MQ=255)
                                                                                                         ggCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGAc                                                           >  1:1526970/1‑136 (MQ=255)
                                                                                                           cTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAAt                                           <  1:29111/150‑1 (MQ=255)
                                                                                                             gACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGc                                         <  2:59429/150‑1 (MQ=255)
                                                                                                              atgTTGGCCATTGGCCTGCGCGCCAT‑CCTGCGTCAGCACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGCg                                        <  2:2200932/148‑1 (MQ=255)
                                                                                                              aCGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATgag                                        >  1:890930/1‑148 (MQ=255)
                                                                                                              aCGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGCg                                        <  1:1816338/150‑1 (MQ=255)
                                                                                                              aCGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGCg                                        <  1:585304/150‑1 (MQ=255)
                                                                                                                 tttGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGCGGTa                                     >  1:1054464/1‑150 (MQ=255)
                                                                                                                  ttGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGCGGTAg                                    <  1:1716608/150‑1 (MQ=255)
                                                                                                                  ttGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGCGGTAg                                    <  1:53663/150‑1 (MQ=255)
                                                                                                                       aGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGCGGTAGGCGCa                               >  1:1754131/1‑150 (MQ=255)
                                                                                                                        gTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGCGGTAGGCGCAg                              <  2:2235031/150‑1 (MQ=255)
                                                                                                                           gCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGCGGTAGGCGCAGTGa                           >  2:1079926/1‑150 (MQ=255)
                                                                                                                             cTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATAATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGTCAGCATCACCGGCCACCTGGTGCTGTCGACCTTTCACACCGACAATGCGGTAGGCGCAGTGGcc                         >  1:214971/1‑150 (MQ=255)
                                                                                                                                      aT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGCGGTAGGCGCAGTGACCCGCCTgcgc                <  2:1054464/150‑1 (MQ=255)
                                                                                                                                       t‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGATCTTGCACACCAACAATGCGGTAGGCGCAGTGACCCGCCTGcgcg               >  1:1365298/1‑150 (MQ=255)
                                                                                                                                       t‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGCGGTAGGCGCAGTGACCCGCCTGcgcg               <  1:766837/150‑1 (MQ=255)
                                                                                                                                          tcacGTCAGGGTCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGCGGTa                                     >  2:1038594/4‑126 (MQ=255)
                                                                                                                                          tcacGTCAGGGTCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGCGGTa                                     <  1:1038594/123‑1 (MQ=255)
                                                                                                                                          cTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGAGCGCCGTGCAGGCCAGCGTCACCGGCCACCTGGTGCTGTCGACCTTGCACACGAACAATGCGGTAGGCGCAGTGACCCGCCTGCGCGAc             >  2:1092759/1‑150 (MQ=255)
                                                                                                                                           tGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGCGGTAGGCGCAGTGACCCGCCTGCGCGACa            >  2:1757148/1‑150 (MQ=255)
                                                                                                                                            acGTCAGGGTCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGCGGTAGGCGCAGTGACCCGCCTGCGCGACAt           >  1:952692/2‑150 (MQ=255)
                                                                                                                                              gTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACAcc                                                 <  2:2130995/110‑1 (MQ=255)
                                                                                                                                              gTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACAcc                                                 >  1:2130995/1‑110 (MQ=255)
                                                                                                                                                 aGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGca                                                     <  1:1935589/103‑1 (MQ=255)
                                                                                                                                                 aGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGca                                                     >  2:1935589/1‑103 (MQ=255)
                                                                                                                                                 aGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGCGGTAGGCGCAGTGACCCGCCTGCGCGACATGGGCa      >  2:584339/1‑150 (MQ=255)
                                                                                                                                                 aGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGCGGTAGGCGCAGTGACCCGCCTGCGCGACATGGGCa      >  2:723513/1‑150 (MQ=255)
                                                                                                                                                  ggACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGCGGTAGGCGCAGTGACCCGCCTGCGCGACATGGGCAt     <  1:2238557/150‑1 (MQ=255)
                                                                                                                                                     cccTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGCGGTAGGCGCAGTGACCCGCCTGCGCGACATGGGCATCGa  >  2:2343222/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCTGAACAGCCTCAACGATGGCAGCCGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGCCATCGCCGGCATTGGCCAAACCGCCATCAACCCGCGTGCCGGGCTGACGTTTGCCAGTGGCCTGCGCGCCAT‑CCTGCGTCAGGACCCTGACGTGATCATGCTCGGCGAAATCCGCGATCAGGAGACTGCGCAGATCGCCGTGCAGGCCAGCCTCACCGGCCACCTGGTGCTGTCGACCTTGCACACCAACAATGCGGTAGGCGCAGTGACCCGCCTGCGCGACATGGGCATCGA  >  NC_002947/1198475‑1198772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑