Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,289,857	A→T	6.5%	N375I (AAT→ATT)	PP_1126 →	hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,289,857	0	A	T	6.5%	289.5 / 9.0	92	N375I (AAT→ATT)	PP_1126	hydrolase
Reads supporting (aligned to +/- strand):  ref base A (48/38);  new base T (3/3);  total (51/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.80e-01

GCACCCGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGCCGACCGC  >  NC_002947/1289708‑1290005                                                                                                                                                      |                                                                                                                                                      gCACCCGTTCCATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCaa                                                                                                                                                       >  2:2059550/1‑150 (MQ=255)   aCCCGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATg                                                                                                                                                     >  2:1857926/1‑150 (MQ=255)       gTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAATGCATGTTCAACGCCTTGCCCGCTGAGGCCGATGCACTGGCCGCTTTCTATGGCGCAAGCAACAACGACGATGGCAATGCC‑tc                                                                                                                                                <  1:574645/150‑1 (MQ=255)        ttCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGACCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGA‑GGCATTGccatcg                                                                                                                                               >  2:1391907/1‑146 (MQ=255)          cTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑tcgtc                                                                                                                                             <  1:1155229/150‑1 (MQ=255)           tATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAGCGACGATTGCAATGCC‑tcgtcg                                                                                                                                            >  2:103937/1‑150 (MQ=255)             ttCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCa                                                                                                                                          >  2:714028/1‑150 (MQ=255)              tCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCaa                                                                                                                                         <  1:1700229/150‑1 (MQ=255)              tCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCAGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGACAGCAACAACGACGATGGCAATGCC‑TCGTCGCaa                                                                                                                                         <  1:2232187/150‑1 (MQ=255)              tCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGACCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGA‑GGCATTGCCAtcgtcgttg                                                                                                                                         <  1:1391907/150‑4 (MQ=255)                ccTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAgg                                                                                                                                       <  1:2367397/150‑1 (MQ=255)                ccTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAgg                                                                                                                                       >  2:1747064/1‑150 (MQ=255)                ccTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACATATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAgg                                                                                                                                       >  1:2108697/1‑150 (MQ=255)                  tGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGgc                                                                                                                                     <  1:1453598/150‑1 (MQ=255)                       gTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAAt                                                                                                                                >  1:1927289/1‑150 (MQ=255)                          aaCGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTTCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTc                                                                                                                             >  2:583474/1‑150 (MQ=255)                          aaCGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGA‑GGCATTGCCAtcgtcgttgtt                                                                                                                                       <  1:1315591/140‑6 (MQ=255)                          aaCGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGA‑GGCATTGCCAtcgtcgttgtt                                                                                                                                       >  2:1315591/1‑135 (MQ=255)                                gTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGTGCGCAATTTCAAGCAg                                                                                                                       <  1:1340222/150‑1 (MQ=255)                                gTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAg                                                                                                                       <  1:169171/150‑1 (MQ=255)                                gTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAg                                                                                                                       <  1:1857926/150‑1 (MQ=255)                                     ggcAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGgcg                                                                                                                  >  1:1544607/1‑150 (MQ=255)                                     ggcAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGgcg                                                                                                                  >  1:1076588/1‑150 (MQ=255)                                     ggcAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGA‑GGCATTGCCAtcgtcg                                                                                                                                            >  2:1681186/1‑124 (MQ=255)                                     ggcAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGA‑GGCATTGCCAtcgtcg                                                                                                                                            <  1:1681186/124‑1 (MQ=255)                                              ggggcgGACCTATGTCGCCGGGCGCGAGGGCATGTGCAAGGCCTGGCTCGCGGAGGGCGATGCACTGGCCGCTTTCTAGGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGa                                                                                                         <  1:1083800/149‑1 (MQ=255)                                                     aCCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGATGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTAc                                                                                                  <  2:735038/150‑1 (MQ=255)                                                     aCCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTAc                                                                                                  >  2:1574346/1‑150 (MQ=255)                                                      ccTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACCTCTAcg                                                                                                 >  1:2199064/1‑150 (MQ=255)                                                       cTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTAcgc                                                                                                >  1:2082461/1‑150 (MQ=255)                                                               ccGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAAcc                                                                                        <  1:427489/150‑1 (MQ=255)                                                                    cgcgAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAg                                                                                   <  1:999203/150‑1 (MQ=255)                                                                     gcgAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGAc                                                                                                        >  1:750444/1‑128 (MQ=255)                                                                     gcgAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGAc                                                                                                        <  2:750444/128‑1 (MQ=255)                                                                       gAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAgg                                                                                                                                       <  2:1032104/95‑1 (MQ=255)                                                                       gAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAgg                                                                                                                                       >  1:1032104/1‑95 (MQ=255)                                                                       gAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGt                                                                                >  2:438254/1‑150 (MQ=255)                                                                         aGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTAc                                                                              <  1:2059550/150‑1 (MQ=255)                                                                             aTGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTAc                                                                              >  1:1958832/1‑146 (MQ=255)                                                                             aTGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTAc                                                                              <  2:1958832/146‑1 (MQ=255)                                                                             aTGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTAt                                                                          >  2:2184033/1‑150 (MQ=255)                                                                             aTGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTAt                                                                          >  2:2302964/1‑150 (MQ=255)                                                                             aTGTTCAAGGCCTGGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTAGGCCGCCAACCTGCAGCGTACCTAt                                                                          >  2:729930/1‑150 (MQ=255)                                                                              tGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATg                                                                         <  1:338128/150‑1 (MQ=255)                                                                               gTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATgg                                                                        >  2:1722303/1‑150 (MQ=255)                                                                                ttCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGc                                                                       >  2:1684245/1‑150 (MQ=255)                                                                                ttCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGc                                                                       >  1:307550/1‑150 (MQ=255)                                                                                   aaGGCCTTGCCCGCTGAGGGCGCTGCGCGGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCaaa                                                                    >  1:931642/1‑150 (MQ=255)                                                                                   aaGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCaaa                                                                    >  2:1963677/1‑150 (MQ=255)                                                                                    aGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAAc                                                                   <  1:321052/150‑1 (MQ=255)                                                                                    aGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGAGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAAc                                                                   >  2:1474516/1‑150 (MQ=255)                                                                                      gCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTAc                                                                                                  >  1:1245258/1‑117 (MQ=255)                                                                                      gCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTAc                                                                                                  <  2:1245258/117‑1 (MQ=255)                                                                                         ttGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCCATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGc                                                              >  2:1783199/1‑150 (MQ=255)                                                                                         ttGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGc                                                              <  1:729930/150‑1 (MQ=255)                                                                                         ttGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGc                                                              >  1:48987/1‑150 (MQ=255)                                                                                         ttGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGc                                                              >  2:684628/1‑150 (MQ=255)                                                                                           gCCCGCTGAGGGCGATGTACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCg                                                            >  2:1229613/1‑150 (MQ=255)                                                                                             ccGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGc                                                          >  1:1724084/1‑150 (MQ=255)                                                                                              cGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGcc                                                         >  2:2347595/1‑150 (MQ=255)                                                                                               gCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGccc                                                        <  1:2347595/150‑1 (MQ=255)                                                                                                  gAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGAGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCa                                                     <  1:1474516/150‑1 (MQ=255)                                                                                                     ggNGATGCACTGGCCGCTTTCTATGGCNAAAGCAACAACGNCGATGGNANTGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTg                                                  >  1:1435476/1‑150 (MQ=255)                                                                                                     ggCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTg                                                  >  1:1639233/1‑150 (MQ=255)                                                                                                       cGATGAGCTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGa                                                <  1:714028/150‑1 (MQ=255)                                                                                                          tGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAggg                                             >  1:359724/1‑150 (MQ=255)                                                                                                           gCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGgc                                            >  2:2401003/1‑150 (MQ=255)                                                                                                            cACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAAc                                                                                         <  2:1668252/104‑1 (MQ=255)                                                                                                            cACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAAc                                                                                         >  1:1668252/1‑104 (MQ=255)                                                                                                             aCTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGgcca                                          <  1:1574346/150‑1 (MQ=255)                                                                                                              cTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGgccag                                         >  2:1198430/1‑150 (MQ=255)                                                                                                                  ccGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTTCCTATGGCAAACCCTGCCGGCCCGCGATGGAGGGccagccag                                     >  2:738858/1‑150 (MQ=255)                                                                                                                  ccGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGccagccag                                     >  2:1992344/1‑150 (MQ=255)                                                                                                                   cGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGgccagcca                                      >  1:311130/1‑148 (MQ=255)                                                                                                                   cGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGgccagcca                                      <  2:311130/148‑1 (MQ=255)                                                                                                                   cGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCcagccagc                                    <  1:583474/150‑1 (MQ=255)                                                                                                                    ggtttCTATGTAGAAATCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCagccagcc                                   <  2:1267186/148‑1 (MQ=255)                                                                                                                       ttCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGAt                                <  2:946927/150‑1 (MQ=255)                                                                                                                        tCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGAtt                               <  2:1927289/150‑1 (MQ=255)                                                                                                                        tCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGAtt                               <  2:2108697/150‑1 (MQ=255)                                                                                                                          tATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTgc                             >  1:339648/1‑150 (MQ=255)                                                                                                                                    gCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCgg                   >  1:2141398/1‑150 (MQ=255)                                                                                                                                      aacaacGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCaaa                                                                    <  1:1460778/99‑1 (MQ=255)                                                                                                                                      aacaacGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCaaa                                                                    >  2:1460778/1‑99 (MQ=255)                                                                                                                                      aacaacGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTg                                                  >  2:603063/1‑117 (MQ=255)                                                                                                                                      aacaacGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTg                                                  <  1:603063/117‑1 (MQ=255)                                                                                                                                          acgacgATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGa                                 >  2:1285278/1‑130 (MQ=255)                                                                                                                                          acgacgATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGa                                 <  1:1285278/130‑1 (MQ=255)                                                                                                                                          acgacgATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTgg             <  1:1229613/150‑1 (MQ=255)                                                                                                                                           cgacgaTGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGa            <  2:2141398/150‑1 (MQ=255)                                                                                                                                               gaTGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGcc        >  2:438041/1‑150 (MQ=255)                                                                                                                                               gaTGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGcc        >  2:7161/1‑150 (MQ=255)                                                                                                                                                 tGGCAATGCC‑TCGTGGCAACGGGGCAGTCCCAAGCCGGTGGGCTGGGTGGATGTCTACGCCGCGAATCTGCAGCGGACCTATGGCAAACCCGGCCGGCCCGCAGTGGAGGGCCAGACCGCCGATTGCCCGCTGCCGGTGCTGGATGCCGa      <  2:759974/150‑1 (MQ=255)                                                                                                                                                    cAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGCCGACCg   >  2:1057312/1‑150 (MQ=255)                                                                                                                                                     aaTGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGCCGACCGc  <  2:307550/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                      GCACCCGTTCTATTCCCTGGGGGTGAACGCCGTGGCGGCAGACGGCGGGCGTACCTATGTCGCCGGGCGCGAAGGCATGTTCAAGGCCTTGCCCGCTGAGGGCGATGCACTGGCCGCTTTCTATGGCGAAAGCAACAACGACGATGGCAATGCC‑TCGTCGCAAGGGCGCAATTTCAAGCAGGGGCGCTGGTTCGACTTCTACGCCGCCAACCTGCAGCGTACCTATGGCAAACCCTGCCGGCCCGCAGTGGAGGGCCAGCCAGCCGATTGCGCGCCGCCGGTGCTGGATGCCGACCGC  >  NC_002947/1289708‑1290005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑