Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 155,546 A→C 5.4% intergenic (‑251/+32) yjbB ← / ← ygdQ Na+/Pi‑cotransporter/transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947155,5460AC 5.4% 218.9 / 4.1 74intergenic (‑251/+32)yjbB/ygdQNa+/Pi‑cotransporter/transport protein
Reads supporting (aligned to +/- strand):  ref base A (31/39);  new base C (2/2);  total (33/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.74e-01

CGCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCGAGAAGGCCATG  >  NC_002947/155402‑155695
                                                                                                                                                |                                                                                                                                                     
cGCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGc                                                                                                                                                  <  2:2024238/150‑1 (MQ=255)
  ccAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGccc                                                                                                                                                <  1:1535250/150‑1 (MQ=255)
   cAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGcc                                                                                                                                                 <  2:1461/148‑1 (MQ=255)
   cAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATGAAAAAGGGCCCTGCGAAGGGcc                                                                                                                                                 >  1:1461/1‑148 (MQ=255)
    aGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCtt                                                                                                                                              <  1:2389732/150‑1 (MQ=255)
    aGCTTCGCATGGCAGTCATCTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTTGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCtt                                                                                                                                              >  2:1234528/1‑150 (MQ=255)
       ttCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCttgtt                                                                                                                                           >  2:1271693/1‑150 (MQ=255)
       ttCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGAAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCttgtt                                                                                                                                           <  1:57201/150‑1 (MQ=255)
         cGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTtgttgt                                                                                                                                         <  2:1079527/150‑1 (MQ=255)
                cAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAGTGAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCAc                                                                                                                                  <  1:2000211/150‑1 (MQ=255)
                    cATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTcc                                                                                                                              <  2:2152558/150‑1 (MQ=255)
                       ttatttGAAACAGGTATTGAAACGAATGACTGCCCGTGGGGGCTTCTTCGCGGGCAAGCGACCACAGTGACAACGCGAAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTg                                                                                                                           <  2:401879/150‑1 (MQ=255)
                          tttGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGcc                                                                                                                        <  1:2115593/150‑1 (MQ=255)
                               aaCAGATCTTGAAACGAATGACTGCCCGCAGTGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTAtt                                                                                                                   >  1:1643626/1‑150 (MQ=255)
                                aCAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGAAAACAACGCTCAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTg                                                                                                                  >  2:196328/1‑150 (MQ=255)
                                  aGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGAc                                                                                                                <  1:1271693/150‑1 (MQ=255)
                                    aTCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGAccc                                                                                                              >  1:795056/1‑150 (MQ=255)
                                     gcTTGAAAAGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAGGGGCCCTGCGAAGGGCCCTTGTGGTGCTGCACTTCCTTGGCCGTATTGACCCg                                                                                                             <  2:969882/149‑1 (MQ=255)
                                       ttGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTa                                                                                                                     <  2:1449953/140‑1 (MQ=255)
                                       ttGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTa                                                                                                                     >  1:1449953/1‑140 (MQ=255)
                                       ttGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCAGa                                                                                                           >  1:166054/1‑150 (MQ=255)
                                               aaTGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCtt                                                                                                   <  1:1895559/150‑1 (MQ=255)
                                                       cccGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCtt                                                                                                                            <  1:1797604/117‑1 (MQ=255)
                                                       cccGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCtt                                                                                                                            >  2:1797604/1‑117 (MQ=255)
                                                       cccGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGtt                                                                                           >  2:1452933/1‑150 (MQ=255)
                                                       cccGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGtt                                                                                           >  2:1505020/1‑150 (MQ=255)
                                                              gggCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCgg                                                                                    <  1:1452933/150‑1 (MQ=255)
                                                                  tgctTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCa                                                                                <  1:800716/150‑1 (MQ=255)
                                                                    ctTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGAcc                                                                                                               <  2:1009257/117‑1 (MQ=255)
                                                                    ctTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGAcc                                                                                                               >  1:1009257/1‑117 (MQ=255)
                                                                    ctTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATg                                                                              >  2:2219487/1‑150 (MQ=255)
                                                                      tCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGAt                                                                            >  1:275141/1‑150 (MQ=255)
                                                                           ggCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGa                                                                             >  1:321837/1‑144 (MQ=255)
                                                                           ggCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGa                                                                             <  2:321837/144‑1 (MQ=255)
                                                                              aaGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGtgc                                                                                                                                       >  2:1998699/1‑83 (MQ=255)
                                                                              aaGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGtgc                                                                                                                                       <  1:1998699/83‑1 (MQ=255)
                                                                              aaGCCACCACAGTGACAACAAAAAAAAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCa                                                                    <  2:1168129/150‑1 (MQ=255)
                                                                                 ccaccaCAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATcc                                                                 >  1:953280/1‑150 (MQ=255)
                                                                                  caccacAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATGCTTGCCATCCt                                                                >  1:1124048/1‑150 (MQ=255)
                                                                                     cacaGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGc                                                             >  2:1645296/1‑150 (MQ=255)
                                                                                     cacaGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATCGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGc                                                             <  1:673913/150‑1 (MQ=255)
                                                                                      acaGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCg                                                            >  1:959977/1‑150 (MQ=255)
                                                                                       caGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCgg                                                           <  1:2219487/150‑1 (MQ=255)
                                                                                       aaGTGACACAGAGCAGGAACCAACGTACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCgg                                                           <  2:1124048/149‑1 (MQ=255)
                                                                                          tGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTc                                                                                 >  2:1283066/1‑125 (MQ=255)
                                                                                          tGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTc                                                                                 <  1:1283066/125‑1 (MQ=255)
                                                                                             cAACGCGCATGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGAACTGCAAACGGCACTTGTAGTGCTGAACTTCCTTAGCCTTATTCATCCGGAATGTCCTTGCGCAGTTTCTCCGGTTCATGATCCTTGCCATCCTTGCGGGCCATg                                                     <  2:275141/150‑1 (MQ=255)
                                                                                             cAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTg                                                                       <  1:68029/132‑1 (MQ=255)
                                                                                             cAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCAACGGTTCATGATCCTTg                                                                       >  2:68029/1‑132 (MQ=255)
                                                                                              aaCGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATgg                                                    >  2:2380479/1‑150 (MQ=255)
                                                                                                 gcgcAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTg                                                                                                                           >  1:565627/1‑76 (MQ=255)
                                                                                                 gcgcAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTg                                                                                                                           <  2:565627/76‑1 (MQ=255)
                                                                                                  cgcAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGt                                                <  1:1645296/150‑1 (MQ=255)
                                                                                                    cAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTgc                                              <  1:1505020/150‑1 (MQ=255)
                                                                                                        aaaaaaCGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCAt                                          >  2:812863/1‑150 (MQ=255)
                                                                                                          aaaaCGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGt                                                                                   >  1:1619926/1‑107 (MQ=255)
                                                                                                          aaaaCGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGt                                                                                   <  2:1619926/107‑1 (MQ=255)
                                                                                                               gCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATg                                   <  2:795056/150‑1 (MQ=255)
                                                                                                                   aGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGa                               >  2:975808/1‑150 (MQ=255)
                                                                                                                              cacaacaaGGGCCCTTCGCAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGAt                              >  1:836464/7‑140 (MQ=255)
                                                                                                                              cacaacaaGGGCCCTTCGCAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGAt                              <  2:836464/134‑1 (MQ=255)
                                                                                                                              aTTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCAcc                    >  2:1496882/1‑150 (MQ=255)
                                                                                                                              aTTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCAcc                    >  1:1660072/1‑150 (MQ=255)
                                                                                                                              aTTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCAGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGTTTTTTTGTATGGTGACGGCTTCCAcc                    <  2:166054/150‑1 (MQ=255)
                                                                                                                                  aaaaGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCa                <  1:2177650/150‑1 (MQ=255)
                                                                                                                                       ggCCCTTCGCAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCa                <  2:1452459/145‑1 (MQ=255)
                                                                                                                                       ggCCCTTCGCAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCa                >  1:1452459/1‑145 (MQ=255)
                                                                                                                                         cccTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCGAGaa         <  1:975808/150‑1 (MQ=255)
                                                                                                                                          ccTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTcc                       <  2:1454393/135‑1 (MQ=255)
                                                                                                                                          ccTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTcc                       >  1:1454393/1‑135 (MQ=255)
                                                                                                                                          ccTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCGAGAAg        <  1:1037479/150‑1 (MQ=255)
                                                                                                                                          ccTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCATTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATg                             <  2:1913031/129‑1 (MQ=255)
                                                                                                                                          ccTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCATTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATg                             >  1:1913031/1‑129 (MQ=255)
                                                                                                                                           cTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCGAGAAgg       <  2:1643626/150‑1 (MQ=255)
                                                                                                                                                aaGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCGAGAAGGCCATg  >  1:1919161/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
CGCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCGAGAAGGCCATG  >  NC_002947/155402‑155695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑