BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,293,266	A→T	7.4%	K288M (AAG→ATG) ‡	PP_1128 →	OmpA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,293,266	0	A	T	7.4%	320.1 / 10.0	104	K288M (AAG→ATG) ‡	PP_1128	OmpA family protein
Reads supporting (aligned to +/- strand): ref base A (45/50); new base T (3/5); total (49/55)
Fisher's exact test for biased strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

CGAGGGCCGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGC  >  NC_002947/1293117‑1293413

tgAGGGCCGCGCCCTGAACCGCCGCGTGGAAATGCTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCaa                                                                                                                                                     <  1:1843444/149‑1 (MQ=255)

cGAGGGCCGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCaa                                                                                                                                                     <  1:1030970/150‑1 (MQ=255)

cGAGGGCCGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCaa                                                                                                                                                     <  1:2168357/150‑1 (MQ=255)

cGAGGGCCGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCaa                                                                                                                                                     <  1:287578/150‑1 (MQ=255)

cGAGGGCCGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCaa                                                                                                                                                     >  1:836726/1‑150 (MQ=255)

      ccGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGCTGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGc                                                                                                                                               >  1:519191/1‑150 (MQ=255)

        gcgcCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCtg                                                                                                                                             >  1:978653/1‑150 (MQ=255)

         cgcCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCtgg                                                                                                                                            <  1:1455536/150‑1 (MQ=255)

         cgcCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCtgg                                                                                                                                            <  1:1162389/150‑1 (MQ=255)

          gcCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCGGCCTTGGCAGCtggt                                                                                                                                           <  1:2257427/150‑1 (MQ=255)

           cccTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCtggtg                                                                                                                                          <  1:1780345/150‑1 (MQ=255)

             cTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCGGCCTTGGCAGCtggtgcc                                                                                                                                        <  1:819027/150‑3 (MQ=255)

              tGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCa                                                                                                                                       >  1:1733869/1‑150 (MQ=255)

               gAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCaa                                                                                                                                      >  2:1913804/1‑150 (MQ=255)

                  ccgccgCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAAcc                                                                                                                                   >  2:1459528/1‑150 (MQ=255)

                   cgccgcGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCt                                                                                                                                  <  2:1301383/150‑1 (MQ=255)

                      cgcgTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGcc                                                                                                                               <  2:2215198/150‑1 (MQ=255)

                           ggAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCaa                                                                                                                          <  2:1024633/150‑1 (MQ=255)

                           ggAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCaa                                                                                                                          <  2:978653/150‑1 (MQ=255)

                           ggAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCaa                                                                                                                          <  1:1123104/150‑1 (MQ=255)

                            gAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAg                                                                                                                         >  1:1035389/1‑150 (MQ=255)

                            gAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAg                                                                                                                         <  2:970710/150‑1 (MQ=255)

                            gAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAg                                                                                                                         <  2:503849/150‑1 (MQ=255)

                             aaaTGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAgg                                                                                                                        <  1:1786813/150‑1 (MQ=255)

                             aaaTGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCGGCCTTGGCAGCtggtgccttg                                                                                                                                     >  1:1766683/1‑132 (MQ=255)

                             aaaTGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCGGCCTTGGCAGCtggtgccttg                                                                                                                                     <  2:1766683/137‑6 (MQ=255)

                                tGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGACCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGc                                                                                                                       <  2:1523750/148‑1 (MQ=255)

                                 gTTGCTGACCCCGCAAACCAACATGGCCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTgc                                                                                                                    <  2:2360803/150‑1 (MQ=255)

                                 gTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTgc                                                                                                                    >  2:1855447/1‑150 (MQ=255)

                                  ttGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCCAGGCCGCTGGTGGCAAACCTCACGTCAAGGCTgcg                                                                                                                   >  1:496241/1‑150 (MQ=255)

                                   tGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTgcgg                                                                                                                  <  2:2374432/150‑1 (MQ=255)

                                    gCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTgcggc                                                                                                                 >  1:1350239/1‑150 (MQ=255)

                                      tGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCga                                                                                                               >  1:1523778/1‑150 (MQ=255)

                                       gACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTTAAGGCTGCGGCgaa                                                                                                              <  2:1639991/150‑1 (MQ=255)

                                          cccGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCtggt                                                                                                                                           >  2:1823551/1‑118 (MQ=255)

                                          cccGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCtggt                                                                                                                                           <  1:1823551/118‑1 (MQ=255)

                                           ccGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCAGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGaaa                                                                                                          >  2:1790464/1‑150 (MQ=255)

                                            cGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTCCCGTCAGGCCTGAGGCGAAGAAAc                                                                                                         >  2:1942474/1‑150 (MQ=255)

                                                 aaCACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTa                                                                                                    <  1:753474/150‑1 (MQ=255)

                                                  acacCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCCGTGCCGGATG‑CAACGCACCAAGCTGCCAACGCCGGTGGTGGTAAACCTGGGGTCAAGTCTGCGGCGAAGAAACCTGTAg                                                                                                   <  2:636121/150‑1 (MQ=255)

                                                    acCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGcc                                                                                                 >  1:1585069/1‑150 (MQ=255)

                                                    acCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGcc                                                                                                 >  2:1127763/1‑150 (MQ=255)

                                                       aTGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAg                                                                                              <  1:387805/150‑1 (MQ=255)

                                                        tGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAgg                                                                                             >  2:1094925/1‑150 (MQ=255)

                                                                   ctgGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCt                                                                                  >  2:476717/1‑150 (MQ=255)

                                                                     ggCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGc                                                                                >  1:2002139/1‑150 (MQ=255)

                                                                       ccAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGACGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGACCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGa                                                                              >  2:755750/1‑150 (MQ=255)

                                                                       acAAATACCAGCAGGCCGCACCGGCACCCGCCCCGGCCTCGATAGCGGCGGTGGAGGATGCGAAGGCACC‑AGCTGCCAGTGCCGCTGGTGGCACACCTGCCTTCACGGCTCCGACGAAGAACCCTGTAGCCACGGCCAAAACGCCTGCGa                                                                              >  2:464042/2‑150 (MQ=255)

                                                                        cAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGTGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGaa                                                                             >  2:604428/1‑150 (MQ=255)

                                                                        cAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAAc                                                                                                                                    <  1:1280501/95‑1 (MQ=255)

                                                                        cAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAAc                                                                                                                                    >  2:1280501/1‑95 (MQ=255)

                                                                        cAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAGGAAACCTGTAGCCAAGGCCAAAACGCCTGCGaa                                                                             >  1:725890/1‑150 (MQ=255)

                                                                         aaaTACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAAc                                                                                                         <  2:2119242/121‑1 (MQ=255)

                                                                         aaaTACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAAc                                                                                                         >  1:2119242/1‑121 (MQ=255)

                                                                          aaTACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGa                                                                           <  1:1127763/150‑1 (MQ=255)

                                                                               cagcagGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCt                                                                                                       >  2:1877872/1‑117 (MQ=255)

                                                                               cagcagGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCt                                                                                                       <  1:1877872/117‑1 (MQ=255)

                                                                               cagcagGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCa                                                                      <  1:476717/150‑1 (MQ=255)

                                                                                    ggCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCaa                                                                 <  2:836726/150‑1 (MQ=255)

                                                                                    ggCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCaa                                                                 <  1:1754846/150‑1 (MQ=255)

                                                                                    ggCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCaa                                                                 <  2:29999/150‑1 (MQ=255)

                                                                                     gcctccCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAg                                                                >  2:837390/1‑150 (MQ=255)

                                                                                     gccgccCCGGCACGCGCCCCGGCCTCGATGGGGGCGGTTCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGAAAACATGCCGACAAGGCTGTGGCGGAGAAAACTGTATCCAACGCCAAAACGCCTGCGAAGACTGCAGCCAAg                                                                >  2:2330118/1‑150 (MQ=255)

                                                                                     gccgccCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCt                                                                                  >  2:2398680/1‑132 (MQ=255)

                                                                                     gccgccCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCt                                                                                  <  1:2398680/132‑1 (MQ=255)

                                                                                     gccgccCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAg                                                                >  2:1660396/1‑150 (MQ=255)

                                                                                         ccccGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGCCGAAGAAACCTGTCGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGaaaa                                                            <  1:1459528/150‑1 (MQ=255)

                                                                                          cccGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGaaaaa                                                           >  1:1181302/1‑150 (MQ=255)

                                                                                              gCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCt                                                       >  2:1508438/1‑150 (MQ=255)

                                                                                                aCCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCAAACGTCGCTGGTTGCAAACCTTCCGTCCAGGCTGCGGCGAAGAAACCTGTATCCAAGTCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTgc                                                     <  1:1855447/150‑1 (MQ=255)

                                                                                                      cccGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCAGCGAAGAAACCTGTAGCCAAGGACAAAACGCCTGCGAAGACTGCAGCCACGAAAAAAGCTGTGCCggg                                               >  2:782789/1‑149 (MQ=255)

                                                                                                          gCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAg                                           >  1:1520493/1‑150 (MQ=255)

                                                                                                            cTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGcc                                         >  1:2104656/1‑150 (MQ=255)

                                                                                                                aTGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCAGAGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTgcc                                     >  1:900314/1‑150 (MQ=255)

                                                                                                                           gCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGc                          >  1:660657/1‑150 (MQ=255)

                                                                                                                             aGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTg                        >  1:297244/1‑150 (MQ=255)

                                                                                                                             aGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTg                        <  2:1733869/150‑1 (MQ=255)

                                                                                                                                   ccAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGGACTTCTTCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTg                  >  1:1171991/1‑150 (MQ=255)

                                                                                                                                    tttgccACC‑AGCGGCCTTGGCAGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGtt                   >  1:439993/6‑148 (MQ=255)

                                                                                                                                    cacggCACC‑AGCTCCCAAGGCCGCTGGTGGCAAACCCGCCGTCACGGCCGAGCTCCGAAAATATAGGGTTGAGGCCAAAACGACTGCCATTACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGa                 <  1:782789/147‑1 (MQ=255)

                                                                                                                                    cAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTa                                                                                                    >  1:308096/1‑67 (MQ=255)

                                                                                                                                    cAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTa                                                                                                    <  2:308096/67‑1 (MQ=255)

                                                                                                                                    cAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGc                                          >  2:301647/1‑125 (MQ=255)

                                                                                                                                    cAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGc                                          <  1:301647/125‑1 (MQ=255)

                                                                                                                                    cAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGa                 <  1:604428/150‑1 (MQ=255)

                                                                                                                                    cAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGa                 >  2:2240728/1‑150 (MQ=255)

                                                                                                                                     aaGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGAc                >  1:884547/1‑150 (MQ=255)

                                                                                                                                     aaGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGAc                <  2:1585069/150‑1 (MQ=255)

                                                                                                                                      aGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACa               <  1:2240728/150‑1 (MQ=255)

                                                                                                                                          aCC‑AGCGGCCTTGGCAGCGGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGAc                                                                          >  2:1296810/1‑87 (MQ=255)

                                                                                                                                           cc‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGaaa          <  1:1921331/150‑1 (MQ=255)

                                                                                                                                            c‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAg         >  1:1675246/1‑150 (MQ=255)

                                                                                                                                            c‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAg         <  2:1181302/150‑1 (MQ=255)

                                                                                                                                               gCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTa       <  1:912413/150‑1 (MQ=255)

                                                                                                                                                  gCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCt    <  1:1790464/150‑1 (MQ=255)

                                                                                                                                                  gCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCt    <  2:1523778/150‑1 (MQ=255)

                                                                                                                                                  gCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTCCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCt    <  2:1350239/150‑1 (MQ=255)

                                                                                                                                                   ccAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGaaa                            <  1:320969/125‑1 (MQ=255)

                                                                                                                                                   ccAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGaaa                            >  2:320969/1‑125 (MQ=255)

                                                                                                                                                    cAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGc  <  1:2330118/150‑1 (MQ=255)

CGAGGGCCGCGCCCTGAACCGCCGCGTGGAAATGTTGCTGACCCCGCAAAACACCATGGTCGCGCTGCTGGCCAAATACCAGCAGGCCGCCCCGGCACCCGCCCCGGCCTCGATGGTGGCGGTGCAGGATGCCAAGGCACC‑AGCTGCCAAGGCCGCTGGTGGCAAACCTGCCGTCAAGGCTGCGGCGAAGAAACCTGTAGCCAAGGCCAAAACGCCTGCGAAGACTGCAGCCAAGAAAAAAGCTGCGCCGGCCAAGCCTGCCGCCAAGAAAGCTGCCGTTGACAAGAAAGTAGCTGC  >  NC_002947/1293117‑1293413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`