Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,350,508 T→A 9.8% K11I (AAA→ATA) ‡ PP_1176 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,350,5080TA 9.8% 271.7 / 16.3 91K11I (AAA→ATA) ‡PP_1176hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (42/40);  new base A (6/3);  total (48/43)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.56e-01

GAGAGCCGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTAGTCCC  >  NC_002947/1350360‑1350656
                                                                                                                                                    |                                                                                                                                                    
gagaGCCGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCAttt                                                                                                                                                     <  2:432797/150‑1 (MQ=255)
 agagCCGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTg                                                                                                                                                    <  1:2361124/150‑1 (MQ=255)
     ccGTTTCTGACGAGAACCGGACTCGGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAg                                                                                                                                                <  2:1160721/150‑1 (MQ=255)
       gTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCAAATGGCAGcc                                                                                                                                              >  1:1841597/1‑150 (MQ=255)
           cTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCAAATGGCAGCCtttt                                                                                                                                          <  2:787138/150‑3 (MQ=255)
            tGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgtt                                                                                                                                         <  1:743583/150‑1 (MQ=255)
            tGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgtt                                                                                                                                         <  1:2388521/150‑1 (MQ=255)
            tGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgt                                                                                                                                          >  1:120566/1‑149 (MQ=255)
            tGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCttgt                                                                                                                                          <  2:120566/149‑1 (MQ=255)
                gagaACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATg                                                                                                                                     >  2:603167/1‑150 (MQ=255)
                gagaACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTACAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATg                                                                                                                                     >  2:850600/1‑150 (MQ=255)
                   aaCGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTg                                                                                                                                                    >  2:165638/1‑132 (MQ=255)
                   aaCGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTg                                                                                                                                                    <  1:165638/132‑1 (MQ=255)
                              gCTATGTGAGCGTTCTTAAGCGCCGGAACACACTATCGCGATGTCCAAAAACCGACACGGTCGACAGCCTGGAGAAACGTCGTGTGCGGCTGGTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGtcaa                                                                                                                       >  2:275455/1‑150 (MQ=255)
                               cTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTCTTGATGCTCAGCCCGGtcaat                                                                                                                      <  1:804606/150‑1 (MQ=255)
                                     gAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGcc                                                                                                                              >  2:1789857/1‑136 (MQ=255)
                                     gAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGcc                                                                                                                              <  1:1789857/136‑1 (MQ=255)
                                                     cGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAc                                                                                                <  2:1831005/150‑1 (MQ=255)
                                                       gAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGtcaat                                                                                                                      <  2:1173249/126‑1 (MQ=255)
                                                       gAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCAccc                                                                                              >  1:2112293/1‑150 (MQ=255)
                                                       gAAAACGTTATCGCGATGTCCAAAAAAAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGtcaat                                                                                                                      >  1:1173249/1‑126 (MQ=255)
                                                          aaCGTTATCGCGATGTCCAAAAACAGGCAAGGTCGACAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTc                                                                                           >  1:1998735/1‑150 (MQ=255)
                                                                 tCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATCCt                                                                                    >  2:434824/1‑150 (MQ=255)
                                                                    cgATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGa                                                                                 <  2:2112293/150‑1 (MQ=255)
                                                                           cAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCa                                                                                                                                                 >  1:1167495/1‑79 (MQ=255)
                                                                           cAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCa                                                                                                                                                 <  2:1167495/79‑1 (MQ=255)
                                                                           cAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACAcg                                                                          >  1:1312959/1‑150 (MQ=255)
                                                                           cAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACAcg                                                                          >  1:1607201/1‑150 (MQ=255)
                                                                           cAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACAcg                                                                          >  2:1814693/1‑150 (MQ=255)
                                                                            aaaaaCAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACAcgc                                                                         >  2:710858/1‑150 (MQ=255)
                                                                              aaaCAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGt                                                                       >  2:392570/1‑150 (MQ=255)
                                                                                 cAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGtctc                                                                    <  1:479277/150‑1 (MQ=255)
                                                                                      aaGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCt                                                                                          <  2:1500236/123‑1 (MQ=255)
                                                                                      aaGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCt                                                                                          >  1:1500236/1‑123 (MQ=255)
                                                                                       aGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAaagaa                                                              >  2:1313812/1‑150 (MQ=255)
                                                                                       aGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAaagaa                                                              <  1:1313812/150‑1 (MQ=255)
                                                                                       aGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAaagaa                                                              <  1:392570/150‑1 (MQ=255)
                                                                                       aGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCCTCCCCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAaagaa                                                              <  2:1998735/150‑1 (MQ=255)
                                                                                          tCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGtt                                                           >  2:1535069/1‑150 (MQ=255)
                                                                                           cGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGttc                                                          <  2:9549/150‑1 (MQ=255)
                                                                                             gCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGttctt                                                        >  2:1571563/1‑150 (MQ=255)
                                                                                                   gTATGAAACGTACTGTTCTACTTCTGCCAGGAAATAAAAAGGCTGCCAATTGGCAGCCTTGTTGATGCTCAGCCCGGTCAAGCAATCCCAGCTCAACGCCCCACCAGTCTGATACTCGATCACACGCGTCTCATAGAAGTTCTTATCctt                                                  <  2:159451/150‑1 (MQ=255)
                                                                                                   gTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCctt                                                  <  1:1535069/150‑1 (MQ=255)
                                                                                                    tAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCcttc                                                 <  1:1814550/150‑1 (MQ=255)
                                                                                                     aGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCcttct                                                >  1:1204074/1‑150 (MQ=255)
                                                                                                       gAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCAAATGGCAGcc                                                                                                                                              >  2:603677/1‑54 (MQ=37)
                                                                                                       gAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCAAATGGCAGcc                                                                                                                                              <  1:603677/54‑1 (MQ=37)
                                                                                                         aaCGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGAt                                                                                <  2:374830/114‑1 (MQ=255)
                                                                                                         aaCGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGAt                                                                                >  1:374830/1‑114 (MQ=255)
                                                                                                         aaCGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCaa                                            >  2:2140312/1‑150 (MQ=255)
                                                                                                            gTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTc                                         >  2:1475848/1‑150 (MQ=255)
                                                                                                             tCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTcc                                        >  1:573220/1‑150 (MQ=255)
                                                                                                              aagattttgcttgtacAAGCCAATAAATAGGCTGCTATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCa                                       <  2:522448/143‑1 (MQ=255)
                                                                                                                ggatctgcttcggcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATg                                     >  2:1299291/4‑150 (MQ=255)
                                                                                                                  ttctgcttctgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGAt                                   >  1:859582/1‑150 (MQ=255)
                                                                                                                    ctgcttctgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGAtct                                 >  1:1771775/1‑150 (MQ=255)
                                                                                                                     tgcttctgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGAtctc                                <  1:525786/150‑1 (MQ=255)
                                                                                                                      gcttctgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCAtagatcgg                               >  1:522448/1‑143 (MQ=255)
                                                                                                                      gcttctgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCg                               <  1:603167/150‑1 (MQ=255)
                                                                                                                        ttcttcCAGCCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCCCAGTCCGGGCAATCAATCCTAGCGCAGCGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCt                             <  2:573220/150‑1 (MQ=255)
                                                                                                                           tgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGtc                                                                                                                         >  2:1715086/1‑55 (MQ=255)
                                                                                                                           tgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGtc                                                                                                                         <  1:1715086/55‑1 (MQ=255)
                                                                                                                           tgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGttct                                                         <  1:1848828/119‑1 (MQ=255)
                                                                                                                           tgcCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGttct                                                         >  2:1848828/1‑119 (MQ=255)
                                                                                                                             ccAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCttcttc                                              <  2:2155201/128‑1 (MQ=255)
                                                                                                                             ccAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCttcttc                                              >  1:2155201/1‑128 (MQ=255)
                                                                                                                               aGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGAtc                                  >  2:1374990/1‑138 (MQ=255)
                                                                                                                               aGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGAtc                                  <  1:1374990/138‑1 (MQ=255)
                                                                                                                               aGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCAt                      <  1:1814693/150‑1 (MQ=255)
                                                                                                                                   aaTAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATcac                                                                             <  2:1201376/91‑1 (MQ=255)
                                                                                                                                   aaTAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATcac                                                                             >  1:1201376/1‑91 (MQ=255)
                                                                                                                                   aaTAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGa                  >  1:1657441/1‑150 (MQ=255)
                                                                                                                                    aTAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGaa                 <  1:850600/150‑1 (MQ=255)
                                                                                                                                      aacaaGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACg               >  1:1063847/4‑150 (MQ=255)
                                                                                                                                       acaaGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCAt                      >  1:2186167/3‑142 (MQ=255)
                                                                                                                                       acaaGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCAt                      <  2:2186167/140‑1 (MQ=255)
                                                                                                                                        aaaGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACggg             >  2:994494/1‑150 (MQ=255)
                                                                                                                                         aaGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCCATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGt            <  1:2140312/150‑1 (MQ=255)
                                                                                                                                         aaGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAAGCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGt            <  2:901207/150‑1 (MQ=255)
                                                                                                                                         aaGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGt            >  1:267869/1‑150 (MQ=255)
                                                                                                                                          aGGCTGCCAAATGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGACGAACAAGTCCACCCTCTAATCCAGCATCTGGCTCAGCCATGGGCAAGGGtt           >  2:1834191/1‑150 (MQ=255)
                                                                                                                                            gCTGCCATTTGTCAGCCTTGTGGCGGCTCAGCCCGGTCAATCTATCCCAGCTCAACGCCCCACCCGTCTGATACTCGCTCAGATGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTgg         <  2:204862/150‑1 (MQ=255)
                                                                                                                                              tGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTa       >  2:2215225/1‑150 (MQ=255)
                                                                                                                                                ccATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTAGt     >  2:2204218/1‑150 (MQ=255)
                                                                                                                                                ccATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTAGt     <  2:859582/150‑1 (MQ=255)
                                                                                                                                                 cATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTAGTc    >  1:2145193/1‑150 (MQ=255)
                                                                                                                                                  aTTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATg                     >  1:1438646/1‑132 (MQ=255)
                                                                                                                                                  aTTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATg                     <  2:1438646/132‑1 (MQ=255)
                                                                                                                                                  aTTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACggg             <  1:1244358/140‑1 (MQ=255)
                                                                                                                                                  aTTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACggg             >  2:1244358/1‑140 (MQ=255)
                                                                                                                                                  aTTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTAGTcc   >  1:179105/1‑150 (MQ=255)
                                                                                                                                                  aTTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTAGTcc   <  1:2260026/150‑1 (MQ=255)
                                                                                                                                                   tttGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTAGTccc  <  1:275455/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAGAGCCGTTTCTGACGAGAACGGGATTCCGCTATGTGAGCGTTCTTAAGCGCCGGAAAACGTTATCGCGATGTCCAAAAACAGGCAAGGTCGGCAGCCGTAGGAAACGTCCTGTTCTGCTTCTGCCAGGCAATAAAAAGGCTGCCATTTGGCAGCCTTGTTGATGCTCAGCCCGGTCAATCAATCCCAGCTCAACGCCCCACCCGTCTGATACTCGATCACACGCGTCTCAAAGAAGTTCTTCTCCTTCTTCAAGTCCATGATCTCGCTCATCCATGGGAACGGGTTGGTAGTCCC  >  NC_002947/1350360‑1350656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑