Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,376,329 A→T 7.0% intergenic (+32/+17) kup → / ← PP_1201 potassium transport system protein Kup/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,376,3290AT 7.0% 294.9 / 9.1 97intergenic (+32/+17)kup/PP_1201potassium transport system protein Kup/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (42/46);  new base T (4/3);  total (47/50)
Fisher's exact test for biased strand distribution p-value = 7.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGCGCTGGCGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCACCTACTG  >  NC_002947/1376180‑1376473
                                                                                                                                                     |                                                                                                                                                 
gTCGCGCTGGCGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCaga                                                                                                                                                   <  2:1806798/150‑1 (MQ=255)
  cgcgCTGGCGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGc                                                                                                                                                 <  1:617369/150‑1 (MQ=255)
    cgcTGGCGGGGCAACCTGTTCGCTTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCTAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCgg                                                                                                                                               <  2:1164309/150‑1 (MQ=255)
     gcTGGCGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGc                                                                                                                                              >  1:965138/1‑150 (MQ=255)
      cTGGCGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGCTATACAGCAGGCTCAGGGCCGCAGAGCGGcc                                                                                                                                             <  1:1780515/150‑1 (MQ=255)
       tGGCGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGccc                                                                                                                                            <  2:2263584/150‑1 (MQ=255)
        ggCGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAAGCTCCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCt                                                                                                                                           <  1:2068241/150‑1 (MQ=255)
          cGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGTCAACTTGCGCTTATTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGa                                                                                                                                         <  2:1770612/150‑1 (MQ=255)
          cGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGa                                                                                                                                         <  1:114782/150‑1 (MQ=255)
          cGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGa                                                                                                                                         <  2:927650/150‑1 (MQ=255)
                 aCCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGt                                                                                                                                  <  1:2080311/150‑1 (MQ=255)
                 aCCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTCCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCTCTGCGGCCCTGa                                                                                                                                         >  2:1725533/1‑143 (MQ=255)
                 aCCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTCCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCTCTGCGGCCCTGa                                                                                                                                         <  1:1725533/143‑1 (MQ=255)
                   cTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTGGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGCTGAGTGTTATATAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCa                                                                                                                                >  2:1271074/1‑150 (MQ=255)
                    tGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAg                                                                                                                               >  2:280556/1‑150 (MQ=255)
                      ttCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGtt                                                                                                                             <  2:1588138/150‑1 (MQ=255)
                         gcgTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTg                                                                                                                          >  1:1484452/1‑150 (MQ=255)
                                   tGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCTCTGCGGCCCTGa                                                                                                                                         >  2:216327/1‑125 (MQ=255)
                                   tGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCTCTGCGGCCCTGa                                                                                                                                         <  1:216327/125‑1 (MQ=255)
                                     aagaaTGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCACCGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCt                                                                                                              >  2:710164/1‑150 (MQ=255)
                                             cAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGc                                                                                                      >  1:534066/1‑150 (MQ=255)
                                                      cTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGcgcg                                                                                             >  2:865399/1‑150 (MQ=255)
                                                            cttcttCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGa                                                                                       <  2:792451/150‑1 (MQ=255)
                                                             ttcttcAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGc                                                                                                                                              >  2:2142823/1‑94 (MQ=255)
                                                             ttcttcAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGc                                                                                                                                              <  1:2142823/94‑1 (MQ=255)
                                                             ttcttcAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGAt                                                                                      >  2:1780339/1‑150 (MQ=255)
                                                             ttcttcAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGAt                                                                                      >  2:2196375/1‑150 (MQ=255)
                                                             ttcttcAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTACTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGAt                                                                                      >  1:1832054/1‑150 (MQ=255)
                                                               cttcAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGa                                                                                                                                         >  2:729643/1‑97 (MQ=255)
                                                               cttcAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGa                                                                                                                                         <  1:729643/97‑1 (MQ=255)
                                                               cttcAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGAtct                                                                                    <  1:1780339/150‑1 (MQ=255)
                                                                ttnAACCTGCCGTTGAACCGGGTGATCNAGCTAGGGACCCNGGTCGANANCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGAtctc                                                                                   >  1:1288073/1‑150 (MQ=255)
                                                                ttcAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGa                                                                                                                                         <  1:170617/96‑1 (MQ=255)
                                                                ttcAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGa                                                                                                                                         >  2:170617/1‑96 (MQ=255)
                                                                ttcAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGAtctc                                                                                   <  2:1246416/150‑1 (MQ=255)
                                                                ttcAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGAtctc                                                                                   <  2:670605/150‑1 (MQ=255)
                                                                     ccTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCaa                                                                              >  1:2011452/1‑150 (MQ=255)
                                                                      cTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAAt                                                                             >  2:1511601/1‑150 (MQ=255)
                                                                            ttGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCg                                                                       >  2:561535/1‑150 (MQ=255)
                                                                               aaCCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCtt                                                                    <  1:280556/150‑1 (MQ=255)
                                                                                 ccGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTgg                                                                  <  1:2196375/150‑1 (MQ=255)
                                                                                  cGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGc                                                                 <  2:2011452/150‑1 (MQ=255)
                                                                                    ggTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGa                                                               <  2:534066/150‑1 (MQ=255)
                                                                                      tGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGt                                                             <  2:965138/150‑1 (MQ=255)
                                                                                           gAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGCGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCgn                                                        <  2:1288073/150‑2 (MQ=255)
                                                                                           gAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCt                                                                                                                           >  2:1660286/1‑83 (MQ=255)
                                                                                           gAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCt                                                                                                                           <  1:1660286/83‑1 (MQ=255)
                                                                                           gAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCgg                                                        <  1:2142956/150‑1 (MQ=255)
                                                                                              cTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCAACAATCAACCGCTTGGCGAGTGCCGGGTa                                                     <  1:1271074/150‑1 (MQ=255)
                                                                                                 gggACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGt‑t                                                 <  1:561535/150‑1 (MQ=255)
                                                                                                    aCCCAGGTCGAGATCTGAATGGGGTTATCCTGCAGCCTCAGGTCCGCAGATCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑tctc                                              <  2:1832054/150‑1 (MQ=255)
                                                                                                      ccAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAg                                                                                                                                        >  2:822569/1‑59 (MQ=255)
                                                                                                      ccAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAg                                                                                                                                        <  1:822569/59‑1 (MQ=255)
                                                                                                      ccAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCtcgt                                            >  1:1560166/1‑150 (MQ=255)
                                                                                                      ccAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCtcgt                                            >  1:1534735/1‑150 (MQ=255)
                                                                                                        aGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCt                                                                                                                                           <  2:1062966/54‑1 (MQ=255)
                                                                                                        aGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCt                                                                                                                                           >  1:1062966/1‑54 (MQ=255)
                                                                                                        aGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCtcgtcg                                          >  2:1327514/1‑150 (MQ=255)
                                                                                                         ggTCGAGATCTGAATGGGGTTATACAGCAGGCTAAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCTCGTCGa                                         >  1:205978/1‑150 (MQ=255)
                                                                                                             gagaTCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTa                                                     <  1:115692/135‑1 (MQ=255)
                                                                                                             gagaTCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTa                                                     >  2:115692/1‑135 (MQ=255)
                                                                                                             gagaTCTGAAAGGGGTTCTACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCTCGTCGAAgtg                                     >  2:2394390/1‑150 (MQ=255)
                                                                                                               gaTCTGAATGGGGTTATACAGCAGGATCAGGCCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCTCGTCGAAgtggt                                   >  2:1480896/1‑150 (MQ=255)
                                                                                                                   tGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCtt                                                                                                                                    <  1:1013967/50‑1 (MQ=255)
                                                                                                                   tGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCtt                                                                                                                                    >  2:1013967/1‑50 (MQ=255)
                                                                                                                   tGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCTCGTCGAAGTGGTGccc                               <  1:734551/150‑1 (MQ=255)
                                                                                                                      aTGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGt                                                      >  2:2153235/1‑125 (MQ=255)
                                                                                                                      aTGGCGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGt                                                      <  1:2153235/125‑1 (MQ=255)
                                                                                                                         gggTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTc                                                                                         <  2:1238806/87‑1 (MQ=255)
                                                                                                                         gggTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTc                                                                                         >  1:1238806/1‑87 (MQ=255)
                                                                                                                           gTTATACAGCAGGCTCAGGGCCGTGCCGCGGCCCTGCGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCCCGTTTCGATCTAACACATCTCCTCCATACCG‑GTGGCCGGTTGTGTCTCGTCGAAGTGGTGCCCGCCAGGCa                       <  1:1022973/150‑1 (MQ=255)
                                                                                                                              ataCAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGAtctc                                                                                   >  2:171290/1‑88 (MQ=255)
                                                                                                                              ataCAGCAGGCTCAGCGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGAtctc                                                                                   <  1:171290/88‑1 (MQ=255)
                                                                                                                               taCAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCTCGTCGAAGTGGTGCCCGCCAGGCAGttt                   <  1:2394390/150‑1 (MQ=255)
                                                                                                                                  agcagGCTCAGGGCCGCAGATCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAg                <  1:1327514/150‑1 (MQ=255)
                                                                                                                                  agcagGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGGGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCTTGTCGAATTAGTGCCCGCCAGGCAGTTTCAg                >  2:564375/1‑150 (MQ=255)
                                                                                                                                  agcagGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGt                                                      <  1:1765107/113‑1 (MQ=255)
                                                                                                                                  agcagGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGt                                                      >  2:1765107/1‑113 (MQ=255)
                                                                                                                                  agcagGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAg                >  1:1475962/1‑150 (MQ=255)
                                                                                                                                  acaagGCTCAGGGCCGCTCTGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCaa                                                                          >  2:1965623/4‑93 (MQ=37)
                                                                                                                                  acaagGCTCAGGGCCGCTCTGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCaa                                                                          <  1:1965623/90‑1 (MQ=37)
                                                                                                                                    cagGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTgg                                                                  <  2:637660/99‑1 (MQ=255)
                                                                                                                                    cagGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTgg                                                                  >  1:637660/1‑99 (MQ=255)
                                                                                                                                       gCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCTCGTCGaa                                        >  2:696872/1‑121 (MQ=255)
                                                                                                                                       gCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCTCGTCGaa                                        <  1:696872/121‑1 (MQ=255)
                                                                                                                                         tCAGGGCCGCTCTGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCa         >  1:1343957/1‑150 (MQ=255)
                                                                                                                                         tCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCa         >  1:2335507/1‑150 (MQ=255)
                                                                                                                                          cAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCAc        <  2:2335507/150‑1 (MQ=255)
                                                                                                                                           aGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TGTATGGGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCAcc       >  1:22710/1‑150 (MQ=255)
                                                                                                                                             ggCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGc                                                                                 <  1:1565389/75‑1 (MQ=255)
                                                                                                                                             ggCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCTCGTCGAAGTGGTGCCCGc                             <  1:959170/126‑1 (MQ=255)
                                                                                                                                             ggCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCTCGTCGAAGTGGTGCCCGc                             >  2:959170/1‑126 (MQ=255)
                                                                                                                                             ggCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCACCTa     >  2:981709/1‑150 (MQ=255)
                                                                                                                                             ggCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTGGGCGCGTTTCGATCTCGc                                                                                 >  2:1565389/1‑75 (MQ=255)
                                                                                                                                               ccGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGACGAGTGCCGGGGAGT‑TCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCACCTACt   >  2:1530972/1‑150 (MQ=255)
                                                                                                                                                cGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCACCTACTg  <  2:1475962/150‑1 (MQ=255)
                                                                                                                                                  cAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTa                                                     <  1:211055/98‑1 (MQ=255)
                                                                                                                                                  cAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTa                                                     >  2:211055/1‑98 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GTCGCGCTGGCGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTTCAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGT‑TCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCACCTACTG  >  NC_002947/1376180‑1376473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑