Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,416,880 T→C 7.5% L41P (CTG→CCG)  PP_1239 → metallo‑beta‑lactamase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,416,8800TC 7.5% 310.7 / 10.8 106L41P (CTG→CCG) PP_1239metallo‑beta‑lactamase family protein
Reads supporting (aligned to +/- strand):  ref base T (55/43);  new base C (5/3);  total (60/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

Only 100 of 109 total aligned reads displayed.

GCTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTG  >  NC_002947/1416735‑1417028
                                                                                                                                                 |                                                                                                                                                     
 cTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCAGCGCCAGGCGCCGCTc                                                                                                                                                   >  2:159684/1‑149 (MQ=255)
 cTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCAGCGCCAGGCGCCGCTc                                                                                                                                                   <  1:159684/149‑1 (MQ=255)
  tATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCgg                                                                                                                                                 >  1:619926/1‑150 (MQ=255)
  tATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCgg                                                                                                                                                 <  1:950510/150‑1 (MQ=255)
  tATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCgg                                                                                                                                                 <  1:1339412/150‑1 (MQ=255)
   aTTCAGGACCCCCTCGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCACAGCGACAGGGTCACCGATCTTCAGTGATGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCggg                                                                                                                                                <  1:2350605/150‑1 (MQ=255)
   aTTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCggg                                                                                                                                                <  2:619926/150‑1 (MQ=255)
   aTTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCGTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCggg                                                                                                                                                <  2:1437733/150‑1 (MQ=255)
       aGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGttt                                                                                                                                            <  2:1154368/150‑1 (MQ=255)
        ggACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGACGACCAGGGAACGGTACGGTGATGGCCAGTTTTGTCACGTTCATCCTTGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTc                                                                                                                                           <  2:1476771/150‑1 (MQ=255)
         gACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCt                                                                                                                                          >  2:761717/1‑150 (MQ=255)
          accaccTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTg                                                                                                                                         <  1:1003755/150‑1 (MQ=255)
               cTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGcagct                                                                                                                                    >  1:30584/1‑150 (MQ=255)
                     tAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCggg                                                                                                                                                >  2:2026065/1‑132 (MQ=255)
                     tAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCggg                                                                                                                                                <  1:2026065/132‑1 (MQ=255)
                        gTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTcagt                                                                                                                           >  1:553010/1‑150 (MQ=255)
                          gcgcTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCAGCGCCAGGCgccgct                                                                                                                                                    >  1:2124607/1‑123 (MQ=255)
                          gcgcTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCAGCGCCAGGCgccgct                                                                                                                                                    <  2:2124607/123‑1 (MQ=255)
                           cgcTTCGCGGTATTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTcagtgca                                                                                                                        <  1:18534/150‑1 (MQ=255)
                            gcTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTcagtgcag                                                                                                                       >  1:994515/1‑150 (MQ=255)
                            gcTCCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATACCTATGGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTcagtgcag                                                                                                                       <  1:1352885/150‑1 (MQ=255)
                             cTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTcagtgcagt                                                                                                                      >  2:1606523/1‑150 (MQ=255)
                                 gcgGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTg                                                                                                                  <  1:364148/150‑1 (MQ=255)
                                 gcgGTACTCGGAAGCCGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTTCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTg                                                                                                                  <  1:52026/150‑1 (MQ=255)
                                    agaCTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTc                                                                                                               >  2:1298533/3‑150 (MQ=255)
                                     tACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCa                                                                                                              >  1:131287/1‑150 (MQ=255)
                                       cTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑cc                                                                                                           <  2:813225/150‑1 (MQ=255)
                                               cGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACa                                                                                                   >  1:1599600/1‑150 (MQ=255)
                                                ggCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACAt                                                                                                  >  1:1105166/1‑150 (MQ=255)
                                                 gCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGcagctca                                                                                                                                  >  2:1583957/1‑118 (MQ=255)
                                                 gCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGcagctca                                                                                                                                  <  1:1583957/118‑1 (MQ=255)
                                                    gCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCg                                                                                              >  2:1883345/1‑150 (MQ=255)
                                                     ccAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTcagtgca                                                                                                                        <  1:1273133/124‑1 (MQ=255)
                                                     ccAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTcagtgca                                                                                                                        >  2:1273133/1‑124 (MQ=255)
                                                        aGGGAACGGCACGCTGATCGCCAGTGCTGACACGTTCATCCTGGTCGATTGCCGCTTTCACCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCAGGGATCCTGCAGCCCAGCTCCGTTCAGTGCTGGTCA‑CCGCCGAACATTTCGAcaa                                                                                          >  2:198099/1‑148 (MQ=255)
                                                               ggCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGTGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCAt                                                                                   <  1:992717/150‑1 (MQ=255)
                                                               ggCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCAt                                                                                   >  2:987433/1‑150 (MQ=255)
                                                               ggCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCAt                                                                                   >  2:2056828/1‑150 (MQ=255)
                                                                 aaCGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGTATAAGGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATgg                                                                                 <  1:761717/149‑1 (MQ=255)
                                                                  aCGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATggg                                                                                <  1:198099/150‑1 (MQ=255)
                                                                    gCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGCTTGGGGGCTTTCGTTTGTTGAAACCGAGCGGCGCCGGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGt                                                                              >  1:1023482/1‑150 (MQ=255)
                                                                      tGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCg                                                                            >  1:1466450/1‑150 (MQ=255)
                                                                      tGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCg                                                                            >  2:1539640/1‑150 (MQ=255)
                                                                        aTCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGc                                                                          >  1:512322/1‑150 (MQ=255)
                                                                         tCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCt                                                                         <  2:553010/150‑1 (MQ=255)
                                                                           gCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTg                                                                       >  1:1605526/1‑150 (MQ=255)
                                                                           gCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGAAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTg                                                                       >  1:2110091/1‑150 (MQ=255)
                                                                            ccAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGaa                                                                                                     <  1:1054246/119‑1 (MQ=255)
                                                                            ccAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGaa                                                                                                     >  2:1054246/1‑119 (MQ=255)
                                                                                tggtgACACGTTCATCCTGGTCGATTGCGGCTTTTCCTTGCGTAAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGATCAGTGCAGTTCTGGTCA‑CCCACGAACAAGCCGACCCCATGCACGTGGTCGGCATgttcgt                                                                  <  2:669753/150‑6 (MQ=255)
                                                                                         gTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTa                                                         >  1:330468/1‑150 (MQ=255)
                                                                                          ttCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTAc                                                        <  1:1883345/150‑1 (MQ=255)
                                                                                             aTCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTa                                                         >  2:1954679/1‑146 (MQ=255)
                                                                                             aTCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGAGCCTGGTGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTa                                                         <  1:1954679/146‑1 (MQ=255)
                                                                                              tCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATg                                                    >  2:1639841/1‑150 (MQ=255)
                                                                                                cTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTCCGTTCACGGGGCTTCAATGTa                                                  <  2:1792498/150‑1 (MQ=255)
                                                                                                 tGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTa                                                  >  2:1688702/1‑149 (MQ=255)
                                                                                                 tGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTa                                                  >  2:1688409/1‑149 (MQ=255)
                                                                                                 tGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTa                                                  <  1:1688409/149‑1 (MQ=255)
                                                                                                   gTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCg                                               >  1:617311/1‑150 (MQ=255)
                                                                                                   gTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCg                                               >  1:1417078/1‑150 (MQ=255)
                                                                                                    tCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCgg                                              <  1:1606523/150‑1 (MQ=255)
                                                                                                      gATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTc                                            >  2:1702259/1‑150 (MQ=255)
                                                                                                         tGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTAc                                         <  2:617311/150‑1 (MQ=255)
                                                                                                           cGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTcagtgc                                                                                                                         >  2:1381903/1‑69 (MQ=255)
                                                                                                           cGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTcagtgc                                                                                                                         <  1:1381903/69‑1 (MQ=255)
                                                                                                           cGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGcc                                                                                               >  1:2371974/1‑94 (MQ=255)
                                                                                                           cGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGcc                                                                                               <  2:2371974/94‑1 (MQ=255)
                                                                                                             gCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTTCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCa                                     >  1:1999449/1‑150 (MQ=255)
                                                                                                             gCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTAGGATTGCTGTCATGGCGCAACCATTGAGCAGTCGACCTCa                                     >  2:1123506/1‑150 (MQ=255)
                                                                                                              cTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTTCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAg                                    >  1:1410228/1‑150 (MQ=255)
                                                                                                              cTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGcc                                                                                               >  2:917273/1‑91 (MQ=255)
                                                                                                              cTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGcc                                                                                               <  1:709549/91‑1 (MQ=255)
                                                                                                              cTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGcc                                                                                               <  1:917273/91‑1 (MQ=255)
                                                                                                              cTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGcc                                                                                               >  2:709549/1‑91 (MQ=255)
                                                                                                               ttttCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGc                                   >  1:358370/1‑150 (MQ=255)
                                                                                                                    ccTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATggg                                                                                >  1:562173/1‑100 (MQ=255)
                                                                                                                    ccTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATggg                                                                                <  2:562173/100‑1 (MQ=255)
                                                                                                                     cTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCACCCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAgg                              >  1:1043548/1‑150 (MQ=255)
                                                                                                                     cTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAggg                             >  1:788626/1‑150 (MQ=255)
                                                                                                                      tGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGa                            <  1:987433/150‑1 (MQ=255)
                                                                                                                          tGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGgc                                                             >  1:1603770/1‑113 (MQ=255)
                                                                                                                          tGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGgc                                                             <  2:1603770/113‑1 (MQ=255)
                                                                                                                             ccccGAGCAGCGCCAGGCGCCGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTgcg                     >  2:1202986/3‑150 (MQ=255)
                                                                                                                              cccGAGCAGCGCCAGGCGCCGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCtt                                                                        <  1:334002/97‑1 (MQ=39)
                                                                                                                              cccGAGCAGCGCCAGGCGCCGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCtt                                                                        >  2:334002/2‑98 (MQ=39)
                                                                                                                              aCCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCAt                                                                                   <  2:1071464/87‑1 (MQ=255)
                                                                                                                              aCCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCAt                                                                                   >  1:1071464/1‑87 (MQ=255)
                                                                                                                              aCCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATgggg                                                                               >  1:169694/1‑91 (MQ=255)
                                                                                                                              aCCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATgggg                                                                               <  2:169694/91‑1 (MQ=255)
                                                                                                                                cGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCgg                  >  1:742742/1‑150 (MQ=255)
                                                                                                                                cGAGCAGCGCCAGGCGCCGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGa                                                                                             <  2:2090629/75‑1 (MQ=38)
                                                                                                                                cGAGCAGCGCCAGGCGCCGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGa                                                                                             >  1:2090629/1‑75 (MQ=38)
                                                                                                                                 gAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGc                 >  2:58632/1‑150 (MQ=255)
                                                                                                                                      gcgcCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCgg                                                                           >  1:1694956/1‑87 (MQ=255)
                                                                                                                                      gcgcCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCgg                                                                           <  2:1694956/87‑1 (MQ=255)
                                                                                                                                      gcgcCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATgcg            >  2:1896172/1‑150 (MQ=255)
                                                                                                                                      gcgcCAGGCGCCGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATgcg            >  2:1101447/1‑150 (MQ=255)
                                                                                                                                          cTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAg        >  1:1443016/1‑150 (MQ=255)
                                                                                                                                                ctgctCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGtt  <  1:1298533/150‑2 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
GCTATTCAGGACCACCTGGGCTAAGTGCGCTTCGCGGTACTCGGAAGCGGCAGCCAAGGGAACGGCACGCTGATCGCCAGTGGTGACACGTTCATCCTGGTCGATTGCGGCTTTTCCCTGCGTGAAACCGAGCGGCGCCTGGCGCTGCTCGGGGTTTCTGCAGCTCAGCTCAGTGCAGTGCTGGTCA‑CCCACGAACATGCCGACCACGTGCATGGGGTCGGCTTGCTGTCACGGCGCTACAATGTACCGGTCTACCTCAGCCAAGGGACTTTGCGCGGCATGCGCAAGCCGGTG  >  NC_002947/1416735‑1417028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑