Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,457,925 C→A 14.5% H93N (CAC→AAC)  PP_1276 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,457,9250CA14.5% 218.6 / 23.2 83H93N (CAC→AAC) PP_1276hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (35/36);  new base A (4/8);  total (39/44)
Fisher's exact test for biased strand distribution p-value = 3.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

GGGAGCGCCGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTC  >  NC_002947/1457784‑1458074
                                                                                                                                             |                                                                                                                                                     
gggAGCGCCGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGACCTCCAGGCGGGCGTGCAGGATGTCGTCATCGGGGTTTAGGCGAAGCGGCCGCTTGTGAACAAgcgc                                                                                                                                               <  2:1233834/150‑1 (MQ=255)
gggAGCGCCGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTGAACAAgcgc                                                                                                                                               <  1:1491697/150‑1 (MQ=255)
gggAGCGCCGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTGAACAAgcgc                                                                                                                                               <  1:1491653/150‑1 (MQ=255)
 ggAGCGCCGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGcgcg                                                                                                                                              <  1:744202/150‑1 (MQ=255)
 ggAGCGCCGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTGAACAAgcgcc                                                                                                                                              <  2:2156970/150‑2 (MQ=255)
 ggAGCGCCGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTGAACAAgcgcc                                                                                                                                              <  2:233947/150‑2 (MQ=255)
   aGCGCCGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGc                                                                                                                                            <  1:390534/150‑1 (MQ=255)
   aGCGCCGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTGAACAAgcgcccg                                                                                                                                            <  2:1166714/150‑4 (MQ=255)
      gcCGAAAAATGAGCGTGGGTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTGAACAAgcgcccggtt                                                                                                                                         <  2:624408/150‑7 (MQ=255)
       ccGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACaa                                                                                                                                        <  2:716229/150‑1 (MQ=255)
       ccGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCAACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACaa                                                                                                                                        >  1:2162773/1‑150 (MQ=255)
        cGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAAc                                                                                                                                       <  2:2128452/150‑1 (MQ=255)
        cGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAAc                                                                                                                                       >  1:590620/1‑150 (MQ=255)
        cGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAAATACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAAc                                                                                                                                       >  2:188218/1‑150 (MQ=255)
            aaaTCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTAt                                                                                                                                   <  2:813579/150‑1 (MQ=255)
                  gCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATggcggc                                                                                                                             <  1:1277172/150‑1 (MQ=255)
                     tGGTTGTACGCGTACAAGGGGCAGGGCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTAt                                                                                                                          <  2:893286/150‑1 (MQ=255)
                      ggTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTGAACAAgcgcc                                                                                                                                              >  2:1379171/1‑128 (MQ=255)
                      ggTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTGAACAAgcgcc                                                                                                                                              <  1:1379171/129‑2 (MQ=255)
                              gcgTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACaa                                                                                                                                                   <  2:895158/116‑1 (MQ=255)
                              gcgTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACaa                                                                                                                                                   >  1:895158/1‑116 (MQ=255)
                               cgTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTAGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCg                                                                                                                <  1:2030554/150‑1 (MQ=255)
                                  aCAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGc                                                                                                             <  1:1877988/150‑1 (MQ=255)
                                  aCAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGc                                                                                                             <  2:1908460/150‑1 (MQ=255)
                                   cAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCg                                                                                                            >  1:1590782/1‑150 (MQ=255)
                                    aaGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGATGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGt                                                                                                           >  1:1354803/1‑150 (MQ=255)
                                       gggCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATc                                                                                                        >  2:182632/1‑150 (MQ=255)
                                         gCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCg                                                                                                      >  2:620618/1‑150 (MQ=255)
                                         gCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGCGGCTGAGGCGAACCCGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGTGCTTATCCg                                                                                                      >  1:1748532/1‑150 (MQ=255)
                                             gCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGaaa                                                                                                  >  1:1959805/1‑150 (MQ=255)
                                               cGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGa                                                                                                    <  2:1315981/146‑1 (MQ=255)
                                               cGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGa                                                                                                    >  1:1315981/1‑146 (MQ=255)
                                                       cGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGa                                                                                        <  1:182632/150‑1 (MQ=255)
                                                       cGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGa                                                                                        <  2:1354803/150‑1 (MQ=255)
                                                        ggACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGAc                                                                                       >  1:1801542/1‑150 (MQ=255)
                                                         gACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACt                                                                                      <  1:1579725/150‑1 (MQ=255)
                                                             tCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCca                                                                                  >  2:1637311/1‑150 (MQ=255)
                                                              cTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATTCGGAAACTCGAAATGACTGCcac                                                                                 >  1:844002/1‑150 (MQ=255)
                                                               tACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCcaca                                                                                >  2:1142215/1‑150 (MQ=255)
                                                                      aCTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCa                                                                         >  2:1602537/1‑150 (MQ=255)
                                                                        ttCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAg                                                                        >  1:2221083/1‑149 (MQ=255)
                                                                        ttCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAg                                                                        <  2:2221083/149‑1 (MQ=255)
                                                                           gATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCt                                                                    <  1:1167438/150‑1 (MQ=255)
                                                                             tCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGc                                                                                                                        <  2:2255421/96‑1 (MQ=255)
                                                                             tCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGc                                                                                                                        >  1:2255421/1‑96 (MQ=255)
                                                                             tCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCggg                                                                                                              <  1:1194259/106‑1 (MQ=255)
                                                                             tCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCggg                                                                                                              >  2:1194259/1‑106 (MQ=255)
                                                                             tCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGa                                                                  >  1:2375101/1‑150 (MQ=255)
                                                                             tCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGa                                                                  >  1:481189/1‑150 (MQ=255)
                                                                                ggAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATc                                                               >  2:1923629/1‑150 (MQ=255)
                                                                                 gAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCCGGGCTGAATcc                                                              >  2:1064638/1‑150 (MQ=255)
                                                                                   aCTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCtt                                                            >  2:1449285/1‑150 (MQ=255)
                                                                                   aCTACAGGAGGGAGAGGGGGGTGTCGTAAGCGGGGGTGAGGCGAAGCGTGCGCTGGTCCACCAGCGGGGCACAACCTATGGCGGCTAAGTGAAATCCGGGCGTATCCGGAACCTCGAAAGGACTACCACAAGCATCAAGGCTGAATCCtt                                                            <  2:287722/150‑1 (MQ=255)
                                                                                     tACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCg                                                          >  2:1154602/1‑150 (MQ=255)
                                                                                     tACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCg                                                          <  2:1959805/150‑1 (MQ=255)
                                                                                     tACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCg                                                          >  1:1919448/1‑150 (MQ=255)
                                                                                        aggaggGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATggcggc                                                                                                                             <  2:10087/80‑1 (MQ=255)
                                                                                        aggaggGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATggcggc                                                                                                                             >  1:10087/1‑80 (MQ=255)
                                                                                        aggaggGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTAt                                                                                                                          <  2:1351379/83‑1 (MQ=255)
                                                                                        aggaggGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTAt                                                                                                                          >  1:1351379/1‑83 (MQ=255)
                                                                                         ggaggGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAAtt                                                      >  2:1989897/1‑150 (MQ=255)
                                                                                             ggCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATTACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGaa                                                  <  2:931154/150‑1 (MQ=255)
                                                                                              gCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAg                                                 <  1:1602537/150‑1 (MQ=255)
                                                                                                gTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGCAggg                                               <  2:1801542/150‑1 (MQ=255)
                                                                                                gTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAggg                                               <  2:590620/150‑1 (MQ=255)
                                                                                                  gCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGgga                                             <  2:1590782/150‑1 (MQ=255)
                                                                                                      gATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGgat                                         <  2:1919448/150‑1 (MQ=255)
                                                                                                       aTGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGgatg                                        <  2:554081/150‑1 (MQ=255)
                                                                                                       aTGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAACATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGgatg                                        <  2:554396/150‑1 (MQ=255)
                                                                                                          tcgtcATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGAtt                                     >  2:2258970/1‑150 (MQ=255)
                                                                                                            gtcATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTg                                   >  1:1342087/1‑150 (MQ=255)
                                                                                                              cATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCa                                 <  1:1989897/150‑1 (MQ=255)
                                                                                                                tCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGc                               >  2:1982562/1‑150 (MQ=255)
                                                                                                                  gggggTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGa                             >  2:717163/1‑150 (MQ=255)
                                                                                                                             gAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGggt                  <  2:1497413/150‑1 (MQ=255)
                                                                                                                              aaCCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGggtg                 <  1:620618/150‑1 (MQ=255)
                                                                                                                               aCCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGggtgg                >  2:1651382/1‑150 (MQ=255)
                                                                                                                                  ccgcgcTTGTGAACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTAGGATTTGAAGGGGAGGATGATTTGCAACGACCTTTCAGGGtggtgg             >  1:1013447/3‑150 (MQ=255)
                                                                                                                                    gcgcTTGTGAACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTggtgggt           >  2:1400022/1‑150 (MQ=255)
                                                                                                                                    gcgcTTGTGAACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATATGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTggtgggt           >  2:1542004/1‑150 (MQ=255)
                                                                                                                                        ttGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGaaa                                                                                           >  2:1236408/1‑66 (MQ=255)
                                                                                                                                        ttGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGaaa                                                                                           <  1:1236408/66‑1 (MQ=255)
                                                                                                                                           ttCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCAt    <  1:2258970/150‑1 (MQ=255)
                                                                                                                                             cacaAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCtt                                                            <  2:2345861/92‑1 (MQ=255)
                                                                                                                                             cacaAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCtt                                                            >  1:2345861/1‑92 (MQ=255)
                                                                                                                                             cacaAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTc  <  1:188218/150‑1 (MQ=255)
                                                                                                                                             cacaAGCGCGGCACAACCTATGGCGGCTATGAGAAATCCGGGCACCTCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTc  <  1:1142215/150‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                     
GGGAGCGCCGAAAAATCAGCGTGGTTGTACGCGTACAAGGGGCAGGCCGAAGATGCGGACGTCTACATTGACTTCGATCTGGAACTACAGGAGGGCGTGCGGGATGTCGTCATCGGGGGTGAGGCGAACCGGGCGCTTGTTCACAAGCGCGGCACAACCTATGGCGGCTATGCGAAATCCGGGCGTATCCGGAAACTCGAAATGACTGCCACAAGCATCAGGGCTGAATCCTTCGAATTTGAAGGGGAGGATGATTTGCAGCGACCTTTCAGGGTGGTGGGTGGCCCATTC  >  NC_002947/1457784‑1458074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑