Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,478,203 T→G 6.0% S284A (TCC→GCC)  PP_1291 → PhoH family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,478,2030TG 6.0% 329.1 / 9.9 116S284A (TCC→GCC) PP_1291PhoH family protein
Reads supporting (aligned to +/- strand):  ref base T (59/50);  new base G (4/3);  total (63/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

Only 100 of 117 total aligned reads displayed.

GAACTGCTGCTACTCGACCTGCACCAGGAGCCTTTGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCGGGTACCGAAGCAGAGAAGATGGAGCCG  >  NC_002947/1478056‑1478352
                                                                                                                                                   |                                                                                                                                                     
 aaCTGCTGCTACTCGACCTGCACCAGGAGCCTTTGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCg                                                                                                                                                    <  2:1220089/150‑1 (MQ=255)
  aCTGCTGCTACTCGACCTGCACCAGGAGCCTTTGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCgg                                                                                                                                                   <  1:519344/150‑1 (MQ=255)
            ctcgcggtGCACCAGGAGCCTTTGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCATGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATc                                                                                                                                         <  2:1073337/143‑1 (MQ=255)
            cTCGACCTGCACCAGGAGCCTTTGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGAGCCGGCGGCGCCGGtcag                                                                                                                                               <  1:249271/144‑4 (MQ=255)
            cTCGACCTGCACCAGGAGCCTTTGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGAGCCGGCGGCGCCGGtcag                                                                                                                                               >  2:249271/1‑141 (MQ=255)
             tCGACCTGCACCAGGAGCCTTTGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATcc                                                                                                                                        >  2:1950159/1‑150 (MQ=255)
                  cTGCACCAGGAGCCTTTGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCa                                                                                                                                   >  1:1097588/1‑150 (MQ=255)
                        cAGGAGCCTTTGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTc                                                                                                                                                      <  1:2142792/125‑1 (MQ=255)
                        cAGGAGCCTTTGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTc                                                                                                                                                      >  2:2142792/1‑125 (MQ=255)
                        cAGGAGCCTGTGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCt                                                                                                                             >  2:1243965/1‑150 (MQ=255)
                         aGGAGCCTTTGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTg                                                                                                                            >  1:1006475/1‑150 (MQ=255)
                          ggAGCCTTTGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTgg                                                                                                                           >  1:589630/1‑149 (MQ=255)
                            aGCCTTTGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCgg                                                                                                                         <  2:1615954/150‑1 (MQ=255)
                               cTTTGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGccc                                                                                                                      >  1:2249918/1‑149 (MQ=255)
                                tttGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCAt                                                                                                                     >  2:19813/1‑150 (MQ=255)
                                 ttGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATc                                                                                                                    <  1:707298/150‑1 (MQ=255)
                                 ttGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATc                                                                                                                    <  2:86818/150‑1 (MQ=255)
                                         ccAGGAAGCCTGGGGCCTGAAACCACGCGATATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAAc                                                                                                            >  1:65268/1‑150 (MQ=255)
                                          cAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAAcc                                                                                                           >  2:1489528/1‑150 (MQ=255)
                                           aGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCa                                                                                                          >  1:875365/1‑150 (MQ=255)
                                            ggAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGa                                                                                                                  >  2:308572/1‑141 (MQ=255)
                                            ggAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCAt                                                                                                         <  1:1950159/150‑1 (MQ=255)
                                             gAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAAc                                                                                                                                             >  2:2004364/1‑113 (MQ=255)
                                             gAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATg                                                                                                        <  2:875365/150‑1 (MQ=255)
                                             gAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATg                                                                                                        >  1:1059917/1‑150 (MQ=255)
                                               aGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGt                                                                                                      >  2:2229482/1‑150 (MQ=255)
                                                   tGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGc                                                                                                  <  1:1217465/150‑1 (MQ=255)
                                                         cTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATGCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAgcgc                                                                                            >  2:414524/1‑150 (MQ=255)
                                                           gAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTc                                                                                                                                                      >  2:698371/1‑90 (MQ=255)
                                                           gAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTa                                                                                          >  1:831316/1‑150 (MQ=255)
                                                              aCCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCt                                                                                                                             <  1:2333711/112‑1 (MQ=255)
                                                              aCCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCt                                                                                                                             >  2:2333711/1‑112 (MQ=255)
                                                              aCCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGc                                                                                                                >  1:1402572/1‑125 (MQ=255)
                                                                 aCGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATgg                                                                                                       <  1:2292565/131‑1 (MQ=255)
                                                                 aCGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATgg                                                                                                       >  2:2292565/1‑131 (MQ=255)
                                                                 aCGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGgcg                                                                                    >  2:1967282/1‑150 (MQ=255)
                                                                  cgagACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCGGGTGAACCTGACCGGGGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGGGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGgcgc                                                                                   >  2:1320138/4‑150 (MQ=255)
                                                                        aTTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCatcatc                                                                             <  2:1059917/150‑1 (MQ=255)
                                                                         ttCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCg                                                                            >  2:1433611/1‑150 (MQ=255)
                                                                          tcatcaAAGCCTGGCGCTGGTCGCCCTGCGGGACCCGTATATCCACCTGGTAAACCTTACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATTGTCAGCAAGCGCTACCGGCGCatcatctc                                                                           <  2:65268/150‑3 (MQ=255)
                                                                            atcaAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCg                                                                                       >  2:1029479/1‑136 (MQ=255)
                                                                            atcaAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCg                                                                                       <  1:1029479/136‑1 (MQ=255)
                                                                             tcaAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCAc                                                                        >  2:2173883/1‑150 (MQ=255)
                                                                                  gCCTGGCGCTGGTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCa                                                                   <  2:174100/150‑1 (MQ=255)
                                                                                    cTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGc                                                                 >  1:2151370/1‑150 (MQ=255)
                                                                                          cTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAg                                                           >  1:1047328/1‑150 (MQ=255)
                                                                                          cTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAg                                                           <  2:670665/150‑1 (MQ=255)
                                                                                           tGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAgg                                                          >  2:1739193/1‑150 (MQ=255)
                                                                                             ttCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAgggg                                                        >  2:768903/1‑150 (MQ=255)
                                                                                             ttCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCACCATCGCCACCCGCAGCGTGCAgggg                                                        >  2:1446634/1‑150 (MQ=255)
                                                                                              tCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCACGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGc                                                       <  1:722221/150‑1 (MQ=255)
                                                                                                gCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTg                                                     >  2:2251915/1‑150 (MQ=255)
                                                                                                gCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTg                                                     >  2:35906/1‑150 (MQ=255)
                                                                                                gCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTg                                                     <  1:1227813/150‑1 (MQ=255)
                                                                                                 cccTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTgg                                                    <  1:2316600/150‑1 (MQ=255)
                                                                                                     gctgGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCa                                                <  1:19813/150‑1 (MQ=255)
                                                                                                      ctgGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAg                                               <  2:841047/150‑1 (MQ=255)
                                                                                                           cccGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGc                                                                           >  1:1296384/1‑117 (MQ=255)
                                                                                                           cccGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGc                                                                           <  2:1296384/117‑1 (MQ=255)
                                                                                                            ccGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGAtt                                         >  1:1555377/1‑150 (MQ=255)
                                                                                                             cGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCAccc                                                                      >  2:501265/1‑120 (MQ=255)
                                                                                                             cGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCAccc                                                                      <  1:501265/120‑1 (MQ=255)
                                                                                                               gATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGc                                      <  1:2173883/150‑1 (MQ=255)
                                                                                                                    ccACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCACCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCt                                 <  1:1446634/150‑1 (MQ=255)
                                                                                                                      aCCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCatcatc                                                                             >  1:1746896/1‑104 (MQ=255)
                                                                                                                      aCCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCatcatc                                                                             <  2:1746896/104‑1 (MQ=255)
                                                                                                                      aCCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGc                               >  1:1049123/1‑150 (MQ=255)
                                                                                                                      aCCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGc                               >  1:1554696/1‑150 (MQ=255)
                                                                                                                       ccTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGcc                              <  2:2151370/150‑1 (MQ=255)
                                                                                                                        cTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGt                                                               >  2:1452463/1‑116 (MQ=255)
                                                                                                                        cTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGt                                                               <  1:1452463/116‑1 (MQ=255)
                                                                                                                        cTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCg                             <  1:1967282/150‑1 (MQ=255)
                                                                                                                         tGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCgg                            <  2:1049123/150‑1 (MQ=255)
                                                                                                                          ggTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCggg                           >  1:1629356/1‑150 (MQ=255)
                                                                                                                           gTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGc                                                                           >  1:1492905/1‑101 (MQ=255)
                                                                                                                           gTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGc                                                                           <  2:1492905/101‑1 (MQ=255)
                                                                                                                           gTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCGGGt                          >  2:2004892/1‑150 (MQ=255)
                                                                                                                              aaCCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCACCACCCGCAGCGTGCAGGGGCTGGATCAgg                                              >  2:1146570/1‑127 (MQ=255)
                                                                                                                                ccTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCgg                            >  1:2137354/1‑143 (MQ=255)
                                                                                                                                ccTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCAGGGTACCGa                     >  1:355806/1‑150 (MQ=255)
                                                                                                                                  tGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCg                             <  2:754363/140‑1 (MQ=255)
                                                                                                                                  tGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCg                             >  1:754363/1‑140 (MQ=255)
                                                                                                                                  tGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCGGGTACCGAAg                   <  1:2004892/150‑1 (MQ=255)
                                                                                                                                    aCCGGAGCCGGCGGCGCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAgc                                                                                              <  2:57163/73‑1 (MQ=38)
                                                                                                                                    aCCGGAGCCGGCGGCGCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAgc                                                                                              >  1:57163/1‑73 (MQ=38)
                                                                                                                                     ccGGAGCCGGCGGCGCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTg                                        <  2:247831/126‑1 (MQ=255)
                                                                                                                                     ccGGAGCCGGCGGCGCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTg                                        >  1:247831/1‑126 (MQ=255)
                                                                                                                                      cGGAGCCGGCGGCGCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGAGGTCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCGGGTACCGAAGCaga               >  1:1953197/1‑150 (MQ=255)
                                                                                                                                       ggCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGcc                              <  1:1906518/134‑1 (MQ=255)
                                                                                                                                       ggCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGcc                              >  2:1906518/1‑134 (MQ=255)
                                                                                                                                       ggCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCGGGTACCGAAGCagag              >  1:2281775/1‑150 (MQ=255)
                                                                                                                                       ggCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTACCGCCGGCTACCGCAgtggcg              >  2:1715272/1‑145 (MQ=255)
                                                                                                                                        gcgcCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCGGGTACCGAAGCAgaga             >  2:670062/1‑150 (MQ=255)
                                                                                                                                            cgccgGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCGGGTAc                        >  1:1404734/1‑135 (MQ=255)
                                                                                                                                            cgccgGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCGGGTAc                        <  2:1404734/135‑1 (MQ=255)
                                                                                                                                             gccgGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCGGGTACCGAAGCAGAGAAGATg        >  1:819928/1‑150 (MQ=255)
                                                                                                                                              ccgGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCGGGTACCGAAGCAGAGAAGATgg       <  2:1629356/150‑1 (MQ=255)
                                                                                                                                                 gCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCGGGTAc                        >  1:1342529/1‑130 (MQ=255)
                                                                                                                                                 gCTCCCGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCGGGTAc                        <  2:1342529/130‑1 (MQ=255)
                                                                                                                                                   tCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCGGGTACCGAAGCAGAGAAGATGGAGCCg  >  2:1048669/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GAACTGCTGCTACTCGACCTGCACCAGGAGCCTTTGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCGGGTACCGAAGCAGAGAAGATGGAGCCG  >  NC_002947/1478056‑1478352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑