Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,594,291 T→C 8.8% T392A (ACC→GCC)  kgtP ← alpha‑ketoglutarate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,594,2910TC 8.8% 311.0 / 14.1 111T392A (ACC→GCC) kgtPalpha‑ketoglutarate permease
Reads supporting (aligned to +/- strand):  ref base T (52/49);  new base C (6/4);  total (58/53)
Fisher's exact test for biased strand distribution p-value = 7.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01

Only 100 of 111 total aligned reads displayed.

CGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCA  >  NC_002947/1594143‑1594439
                                                                                                                                                    |                                                                                                                                                    
cGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTg                                                                                                                                                     <  1:70193/150‑1 (MQ=255)
        aGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACATGGCCACGTATTCGGCGGTGCCGCCGaa                                                                                                                                             <  1:2397618/150‑1 (MQ=255)
         gTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGCACCGCCGaat                                                                                                                                            <  2:1951058/150‑2 (MQ=255)
          tGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGa                                                                                                                                           <  1:591150/150‑1 (MQ=255)
          tGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGa                                                                                                                                           >  2:471500/1‑150 (MQ=255)
               aTCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGac                                                                                                                                      >  1:1628218/1‑150 (MQ=255)
                      aGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGATGCCGCTCTCCATCCCGGCGCTCTTGAACCATTGGTCTTTGTATTCGGCGGTGCCGCCGAAGATCTACACCGTCa                                                                                                                               <  2:68259/150‑1 (MQ=255)
                          ctgctTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGCACCGCCGaatac                                                                                                                                          <  1:659186/135‑4 (MQ=255)
                          ctgctTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGCACCGCCGaatac                                                                                                                                          >  2:659186/1‑132 (MQ=255)
                                ggTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACgg                                                                                                                     >  2:1093824/1‑150 (MQ=255)
                                 gtgtCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGc                                                                                                                    >  1:2124762/1‑150 (MQ=255)
                                      tttCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGcc                                                                                                               >  1:119442/1‑150 (MQ=255)
                                      tttCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGcc                                                                                                               >  1:2185811/1‑150 (MQ=255)
                                        tCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCGCGTGTTGCTGGGCTCATCACAAGATCGACACCGACAGTGCATACTGCAGGCCaa                                                                                                             >  2:1232301/1‑150 (MQ=255)
                                            ggTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGCACCGCCGaa                                                                                                                                             <  2:1308909/114‑1 (MQ=255)
                                            ggTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGCACCGCCGaa                                                                                                                                             >  1:1308909/1‑114 (MQ=255)
                                             gTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGccc                                                                                                        >  2:1316092/1‑150 (MQ=255)
                                                gcgTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAgg                                                                                                     <  1:1454035/150‑1 (MQ=255)
                                                gcgTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAgg                                                                                                     >  2:476257/1‑150 (MQ=255)
                                                gcgTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAgg                                                                                                     <  1:476257/150‑1 (MQ=255)
                                                gcgTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAgg                                                                                                     <  1:518999/150‑1 (MQ=255)
                                                  gTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGCACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCAACACCGTCAGGGCATACGGCATGCCAACGCCCAGGgc                                                                                                   >  2:431333/1‑150 (MQ=255)
                                                  gTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGgc                                                                                                   <  2:2124762/150‑1 (MQ=255)
                                                        cagcagCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAAt                                                                                             >  1:405755/1‑150 (MQ=255)
                                                          gcagcGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAAttt                                                                                           >  1:436635/1‑150 (MQ=255)
                                                            agcGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCg                                                                                         <  1:453428/150‑1 (MQ=255)
                                                            agcGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCg                                                                                         <  1:1360981/150‑1 (MQ=255)
                                                             gcGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCgg                                                                                        <  1:1093824/150‑1 (MQ=255)
                                                              cGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGt                                                                                       >  1:1042050/1‑150 (MQ=255)
                                                               gAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGCCACCGTCCGGAAATAGAGCGGGCGAACGCCGAGTGGGCGAAGTTCGGtg                                                                                      >  2:21794/1‑149 (MQ=255)
                                                                aGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAg                                                                                     <  1:1004874/150‑1 (MQ=255)
                                                                  cAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTc                                                                                          <  1:1880675/143‑1 (MQ=255)
                                                                    ggCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGACCCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGaa                                                                                 >  2:662034/1‑150 (MQ=255)
                                                                    ggCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGaa                                                                                 <  2:1042050/150‑1 (MQ=255)
                                                                    ggCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGaa                                                                                 <  2:1963235/150‑1 (MQ=255)
                                                                     gCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAAc                                                                                >  1:1564705/1‑150 (MQ=255)
                                                                         tACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCt                                                                            >  2:417476/1‑150 (MQ=255)
                                                                            aGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCa                                                                                                                          >  1:2111002/1‑101 (MQ=255)
                                                                            aGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCa                                                                                                                          <  2:2111002/101‑1 (MQ=255)
                                                                                      gTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCAc                                                               >  1:1649628/1‑150 (MQ=255)
                                                                                       tACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAgg                                                                                                                             <  1:1852158/87‑1 (MQ=255)
                                                                                       tACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAgg                                                                                                                             >  2:1852158/1‑87 (MQ=255)
                                                                                       tACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACg                                                              >  1:1692135/1‑150 (MQ=255)
                                                                                       tACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACg                                                              >  1:181020/1‑150 (MQ=255)
                                                                                       tACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGAATACTTCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACg                                                              <  1:431333/150‑1 (MQ=255)
                                                                                          cAGTAGAAGCCGCTCTCCATCCAGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACg                                                              <  1:989657/147‑1 (MQ=255)
                                                                                           agaaGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGt                                                          >  2:399860/4‑150 (MQ=255)
                                                                                            gTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAgg                                                                                                                             >  1:1010431/1‑82 (MQ=255)
                                                                                            gTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAgg                                                                                                                             <  2:1010431/82‑1 (MQ=255)
                                                                                            gTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGtt                                                         <  1:1780634/150‑1 (MQ=255)
                                                                                            gTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGtt                                                         >  2:1743300/1‑150 (MQ=255)
                                                                                              aGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGa                                                       <  1:1316092/150‑1 (MQ=255)
                                                                                              aGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGa                                                       >  1:2153078/1‑150 (MQ=255)
                                                                                                  gCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTc                                                                                          <  2:916480/111‑1 (MQ=255)
                                                                                                  gCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTc                                                                                          >  1:916480/1‑111 (MQ=255)
                                                                                                   ccGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAg                                                  <  1:399860/150‑1 (MQ=255)
                                                                                                    cGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAgg                                                 >  1:646689/1‑150 (MQ=255)
                                                                                                     gCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGCACCGCCGaat                                                                                                                                            <  1:1454324/58‑2 (MQ=38)
                                                                                                     gCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGCACCGCCGaat                                                                                                                                            >  2:1454324/1‑57 (MQ=38)
                                                                                                       tctcCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGtat                                              <  1:417476/150‑1 (MQ=255)
                                                                                                       tctcCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGtat                                              >  1:1557360/1‑150 (MQ=255)
                                                                                                        ctcCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAgg                                                                                                                             <  1:1735033/70‑1 (MQ=255)
                                                                                                        ctcCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAgg                                                                                                                             >  2:1735033/1‑70 (MQ=255)
                                                                                                         tcCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAg                                            >  2:66276/1‑150 (MQ=255)
                                                                                                         tcCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAg                                            >  2:413804/1‑150 (MQ=255)
                                                                                                           cATCCCGGCGCTCTTGAACCACAGGGCTACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGt                                                                                       >  1:1482961/1‑105 (MQ=255)
                                                                                                           cATCCCGGCGCTCTTGAACCACAGGGCTACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGt                                                                                       <  2:1482961/105‑1 (MQ=255)
                                                                                                            aTCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGAATACGGAAGGCCCACGCCATGAGCGCGCATTTCGCTAGGTAACAGCTCGGCTGTGACCACGTCGTTTATCGAGGTATAGCCg                                         <  2:1480430/150‑1 (MQ=255)
                                                                                                             tCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCgg                                                                         >  2:141002/1‑117 (MQ=255)
                                                                                                             tCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCgg                                                                         <  1:141002/117‑1 (MQ=255)
                                                                                                             tCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGc                                        <  2:405755/150‑1 (MQ=255)
                                                                                                               ccGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGACATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCtt                                      >  2:2226189/1‑150 (MQ=255)
                                                                                                                cGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAAAGAAGTAGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTa                                     >  1:1955722/1‑150 (MQ=255)
                                                                                                                   cgcTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACga                                  <  2:1557360/150‑1 (MQ=255)
                                                                                                                   cgcTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACga                                  <  2:1692135/150‑1 (MQ=255)
                                                                                                                   cgcTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACga                                  <  2:2153078/150‑1 (MQ=255)
                                                                                                                       ctTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACgatgat                              >  2:284856/1‑150 (MQ=255)
                                                                                                                       ctTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACgatgat                              <  2:646689/150‑1 (MQ=255)
                                                                                                                        ttGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTa                                                                                      <  2:402268/93‑1 (MQ=255)
                                                                                                                        ttGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTa                                                                                      >  1:402268/1‑93 (MQ=255)
                                                                                                                              cacaGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCg                       >  1:2043152/1‑150 (MQ=255)
                                                                                                                              cacaGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCg                       >  1:260945/1‑150 (MQ=255)
                                                                                                                               acaGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAgg                                                                                    <  2:2087330/88‑1 (MQ=255)
                                                                                                                               acaGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAgg                                                                                    >  1:2087330/1‑88 (MQ=255)
                                                                                                                               acaGGGCCACGTATTCGGCGGTGCCGCCGAAGACCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCgg                      >  2:2384843/1‑150 (MQ=255)
                                                                                                                                caGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAg                                            <  2:277075/127‑1 (MQ=255)
                                                                                                                                caGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAg                                            >  1:277075/1‑127 (MQ=255)
                                                                                                                                 aGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAg                                                  <  1:641224/120‑1 (MQ=255)
                                                                                                                                 aGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAg                                                  >  2:641224/1‑120 (MQ=255)
                                                                                                                                 aGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGcc                    <  1:2040005/150‑1 (MQ=255)
                                                                                                                                 aGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTACGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGcc                    >  1:1621194/1‑150 (MQ=255)
                                                                                                                                     ccACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGa                >  1:2281640/1‑150 (MQ=255)
                                                                                                                                     ccACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGa                <  1:66276/150‑1 (MQ=255)
                                                                                                                                        cGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGTATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCa             <  1:1232301/150‑1 (MQ=255)
                                                                                                                                            ttCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGgaa         <  1:662034/150‑1 (MQ=255)
                                                                                                                                            ttCGGCGGCACCGCCGAAGATCGACACNGTCAGGGCATACNGCAGGCNAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGgaa         >  1:2214069/1‑150 (MQ=255)
                                                                                                                                            ttCGGCGGCACCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGCCGTTGATCGAGGTATAGCCCCTTACGATGATCACTGCGCCCATTCTCAGcaa         >  2:306047/1‑150 (MQ=255)
                                                                                                                                                  ggTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACga                                  <  2:1211039/119‑1 (MQ=255)
                                                                                                                                                  ggTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACga                                  >  1:1211039/1‑119 (MQ=255)
                                                                                                                                                   gTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGACAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCa  <  1:21794/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGTTACTCAGTGGTAATCCGCGAGTGCTGCTTGGTGTCTTTCATGGTCGCGTAAACCAGCAGCGAGCAGGCGATACAGGCGGTGACGTACCAGTAGAAGCCGCTCTCCATCCCGGCGCTCTTGAACCACAGGGCCACGTATTCGGCGGTGCCGCCGAAGATCGACACCGTCAGGGCATACGGCAGGCCAACGCCCAGGGCGCGAATTTCGGTAGGGAACAGCTCGGCTTTGACCACGGCGTTGATCGAGGTATAGCCGCTTACGATGATCAGTGCGGCCATGATCAGGAAGAACGCA  >  NC_002947/1594143‑1594439

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑