Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,667,961 C→T 7.6% A307A (GCC→GCT)  ffh → signal recognition particle GTPase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,667,9610CT 7.6% 333.3 / 11.2 117A307A (GCC→GCT) ffhsignal recognition particle GTPase subunit
Reads supporting (aligned to +/- strand):  ref base C (54/54);  new base T (5/4);  total (59/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

GGTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGACAAGCTGCCGAGC  >  NC_002947/1667823‑1668108
                                                                                                                                          |                                                                                                                                                   
ggTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCAt                                                                                                                                          >  2:1606511/1‑150 (MQ=255)
 gTACGTGCAATCACCGGCAAACCGATCCAGTCCATCGGTATGGGTCAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCCTCCTCGGCATGGGCGACGTGCTCAGCCTGCTCGAGCACGCCGCGCAGACCCTc                                                                                                                                         >  2:781794/1‑150 (MQ=255)
 gTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATc                                                                                                                                         >  2:1632574/1‑150 (MQ=255)
     gTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCGGCCTGCTCGATCAGGCTGa                                                                                                                                                   >  2:1942526/1‑136 (MQ=255)
     gTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCGGCCTGCTCGATCAGGCTGa                                                                                                                                                   <  1:1942526/136‑1 (MQ=255)
      tGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGAcaa                                                                                                                                    >  2:2232104/1‑150 (MQ=255)
      tGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCGCGGCATGGGCGACGTGCGCAGCCTGATCTAGCAGTCCGAGCAGACCATCGAcaa                                                                                                                                    >  2:1932551/1‑150 (MQ=255)
         aaTCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGAcaaggc                                                                                                                                 >  1:1070889/1‑150 (MQ=255)
         aaTCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGAcaaggc                                                                                                                                 >  1:10130/1‑150 (MQ=255)
         aaTCACCGACAAACCGATCAAGTACATCCGTATGGGTGAGAAGAGCGAGGCCCTCGATCAGTTGCACCCCGACCGCGTCGCTTCGCGCATCCTAGACATGCGCGACGTGCTCAGCCTTATCGAGCAGGCCGAGCAGACCATCGAcacggc                                                                                                                                 >  2:97110/1‑150 (MQ=255)
          aTCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGAcaaggcc                                                                                                                                >  1:2381013/1‑150 (MQ=255)
             aCCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGAcaaggccaag                                                                                                                             <  2:1070889/150‑1 (MQ=255)
                 gCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCg                                                                                                                         >  1:1709649/1‑150 (MQ=255)
                   aaaCCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGcc                                                                                                                                                     <  2:527276/120‑1 (MQ=255)
                   aaaCCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGcc                                                                                                                                                     >  1:527276/1‑120 (MQ=255)
                    aaCCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACa                                                                                                                      >  1:2353472/1‑150 (MQ=255)
                        gATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCt                                                                                                                  >  1:859542/1‑150 (MQ=255)
                         aTCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTg                                                                                                                 <  2:2327755/150‑1 (MQ=255)
                          tCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTCCCACCCCTACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTgg                                                                                                                <  2:1644433/150‑1 (MQ=255)
                            aaGGTCAGCGGGATGTGTGAGAAGAAAGAGTCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGcc                                                                                                              >  2:1298385/1‑150 (MQ=255)
                              gTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCaa                                                                                                            >  1:751565/1‑150 (MQ=255)
                                  aTCGGTATGGGTGCGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGaaa                                                                                                        <  2:751565/150‑1 (MQ=255)
                                  aTCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGaaa                                                                                                        <  1:2232104/150‑1 (MQ=255)
                                      gTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTga                                                                                                    <  2:929034/150‑1 (MQ=255)
                                       tATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTgaa                                                                                                   <  2:1381964/150‑1 (MQ=255)
                                        aTGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTgaag                                                                                                  <  2:198240/150‑1 (MQ=255)
                                         tGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTgaaga                                                                                                 >  1:8889/1‑150 (MQ=255)
                                           ggTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGaagaag                                                                                               <  1:1942851/150‑1 (MQ=255)
                                            gTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaagg                                                                                              >  1:1107698/1‑150 (MQ=255)
                                               agaagaCCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaagggca                                                                                           >  1:752854/1‑150 (MQ=255)
                                                gaagaCCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaagggcaa                                                                                          >  1:328296/1‑150 (MQ=255)
                                                 aagaCCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGAcaag                                                                                                                                   >  2:2359511/1‑108 (MQ=255)
                                                 aagaCCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGAcaag                                                                                                                                   <  1:2359511/108‑1 (MQ=255)
                                                 aagaCCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaagggcaag                                                                                         <  2:1709649/150‑1 (MQ=255)
                                                 aagaCCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaagggcaag                                                                                         >  2:1686460/1‑150 (MQ=255)
                                                  agaCCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaagggcaagg                                                                                        <  2:958356/150‑1 (MQ=255)
                                                   gaCCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaagggcaaggg                                                                                       <  1:913599/150‑1 (MQ=255)
                                                      cGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCtt                                                                                    >  2:251567/1‑150 (MQ=255)
                                                       gAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTc                                                                                   <  1:5040/150‑1 (MQ=255)
                                                       gAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTc                                                                                   <  1:251567/150‑1 (MQ=255)
                                                            ccTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCt                                                                              >  1:1685724/1‑150 (MQ=255)
                                                                gAGCCGTTCCACCCCGCCACCGTCGCCTCGCGAATACTCGGCTTGGGCGACGTGCTCCGCCTCATCGCGCAGGCCGAGCAGATCATCGACATGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGaa                                                                          <  2:2308989/150‑1 (MQ=255)
                                                                gAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGCAGAAGGGCAAGGGCTTCGACCTCGaa                                                                          <  1:1092381/150‑1 (MQ=255)
                                                                gAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGaa                                                                          <  2:8889/150‑1 (MQ=255)
                                                                gAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGaa                                                                          <  1:1298385/150‑1 (MQ=255)
                                                                gAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGaa                                                                          <  2:1107698/150‑1 (MQ=255)
                                                                  gCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGa                                                                        <  1:518095/150‑1 (MQ=255)
                                                                  gCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGa                                                                        <  1:661509/150‑1 (MQ=255)
                                                                  gCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCAGCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGa                                                                        <  2:576965/150‑1 (MQ=255)
                                                                     gTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaagggcaag                                                                                         <  2:1338557/130‑1 (MQ=255)
                                                                      ttCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTc                                                                    >  2:1143362/1‑150 (MQ=255)
                                                                        ccACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCcg                                                                  >  2:1962510/1‑150 (MQ=255)
                                                                         cACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCcgc                                                                 <  2:2050762/150‑1 (MQ=255)
                                                                          aCCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCcgcg                                                                >  1:1610662/1‑150 (MQ=255)
                                                                          aCCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCcgcg                                                                >  1:1366536/1‑150 (MQ=255)
                                                                           ccccGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTcc                                                                   >  2:598772/1‑146 (MQ=255)
                                                                           ccccGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTcc                                                                   <  1:598772/146‑1 (MQ=255)
                                                                           ccccGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGa                                                               <  2:1366536/150‑1 (MQ=255)
                                                                           ccccGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGa                                                               >  2:835323/1‑150 (MQ=255)
                                                                             ccGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCa                                                                                                                                                <  1:844958/67‑1 (MQ=255)
                                                                             ccGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCa                                                                                                                                                >  2:844958/1‑67 (MQ=255)
                                                                                aCCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGc                                                          <  2:328296/150‑1 (MQ=255)
                                                                                   gcgTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTgc                                                       <  2:1685724/150‑1 (MQ=255)
                                                                                    cgTCGACTCGCGCAACCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAATGCCAAGGCCGACAAGCTGCCCAAGAAACTGAAGAAGGGCAAGGGCTTCGAGCTCGAAGACTTCCGCGACCAGCTgca                                                      <  2:589116/150‑1 (MQ=255)
                                                                                      tCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTgcagc                                                    >  2:1848151/1‑150 (MQ=255)
                                                                                         cTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCaaa                                                 <  1:1263149/150‑1 (MQ=255)
                                                                                          ttCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAAt                                                <  2:793754/150‑1 (MQ=255)
                                                                                          ttCGCGCATCCTCGGAATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAAt                                                >  1:1686420/1‑150 (MQ=255)
                                                                                              cgcATCCTCGGCATGGGCGACGTGCTCAGCCTGATCCACGCGTCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATgaag                                            <  1:1232347/150‑1 (MQ=255)
                                                                                                  tCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACa                                        >  1:1493422/1‑150 (MQ=255)
                                                                                                    cTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATg                                      >  1:2388695/1‑150 (MQ=255)
                                                                                                    cTCGGCATGGGCGAAGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATg                                      >  2:499353/1‑150 (MQ=255)
                                                                                                     tCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCTATCACCCTACAGCGCACCACCTTGCCACCAAACTGAAGAAGGCCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATgg                                     <  1:781794/150‑1 (MQ=255)
                                                                                                     tCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGaagaag                                                                                               <  1:1066591/92‑1 (MQ=255)
                                                                                                     tCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGaagaag                                                                                               >  2:1066591/1‑92 (MQ=255)
                                                                                                        gCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGggcg                                  <  2:314777/150‑1 (MQ=255)
                                                                                                         cATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCg                                                                            <  1:202046/107‑1 (MQ=255)
                                                                                                             gatggtctGCTCGGCCTGCTCGATCAGGCTGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTc                             >  1:223620/8‑150 (MQ=255)
                                                                                                                 aCGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTcc                                                                   <  1:1319000/108‑1 (MQ=255)
                                                                                                                 aCGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTcc                                                                   >  2:1319000/1‑108 (MQ=255)
                                                                                                                  tctGCTCGGCCTGCTCGATCAGGCTGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGa                                                                        >  1:1793972/3‑102 (MQ=38)
                                                                                                                  cGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTTGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGCCCTCTAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTcggcgg                        <  1:973622/150‑1 (MQ=255)
                                                                                                                    tGCTCGGCCTGCTCGATCAGGCTGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGcc                      >  2:1309991/1‑150 (MQ=255)
                                                                                                                     gCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTgcag                                                     >  1:766975/1‑118 (MQ=255)
                                                                                                                     gCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTgcag                                                     <  2:766975/118‑1 (MQ=255)
                                                                                                                      ctcggCCTGCTCGATCAGGCTGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaagggcaag                                                                                         <  2:1784723/77‑1 (MQ=37)
                                                                                                                      cTCGGCCTGCTCGATCAGGCTGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGaagggcaag                                                                                         >  1:1784723/1‑81 (MQ=37)
                                                                                                                       tCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGaa                                                                          <  2:381657/95‑1 (MQ=255)
                                                                                                                       tCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGaa                                                                          >  1:381657/1‑95 (MQ=255)
                                                                                                                           ccTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATggg               >  1:1098062/1‑149 (MQ=255)
                                                                                                                           ccTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGa               >  2:1331323/1‑150 (MQ=255)
                                                                                                                           ccTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGa               >  2:1331434/1‑150 (MQ=255)
                                                                                                                             tGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGACa             >  1:1214152/1‑150 (MQ=255)
                                                                                                                                tCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGGACGATGGGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGACAAGc          >  1:150899/1‑150 (MQ=255)
                                                                                                                                tCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAACCAGCAGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGACAAGc          <  1:1353620/150‑1 (MQ=255)
                                                                                                                                    gCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCtt                                                                                    <  2:87857/72‑1 (MQ=255)
                                                                                                                                    gCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCtt                                                                                    >  1:87857/1‑72 (MQ=255)
                                                                                                                                      aGGCTGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGACAAGCTGCCGa    <  2:223620/150‑1 (MQ=255)
                                                                                                                                        gCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGACAAGCTGCCGAGc  >  2:1668491/1‑150 (MQ=255)
                                                                                                                                        gCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGACAAGCTGCCGAGc  <  1:1662252/150‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                   
GGTACGTGCAATCACCGGCAAACCGATCAAGTTCATCGGTATGGGTGAGAAGACCGAGGCCCTCGAGCCGTTCCACCCCGACCGCGTCGCTTCGCGCATCCTCGGCATGGGCGACGTGCTCAGCCTGATCGAGCAGGCCGAGCAGACCATCGACAAGGCCAAGGCCGACAAGCTGGCCAAGAAACTGAAGAAGGGCAAGGGCTTCGACCTCGAAGACTTCCGCGACCAGCTGCAGCAAATGAAGAACATGGGCGGCCTCGGCGGCCTGATGGACAAGCTGCCGAGC  >  NC_002947/1667823‑1668108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑