BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,704,678	T→A	11.9%	M1M (TTG→ATG) †‡	PP_1499 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,704,678	0	T	A	11.9%	319.3 / 29.3	119	M1M (TTG→ATG) †‡	PP_1499	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (42/61); new base A (6/8); total (48/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

Only 100 of 120 total aligned reads displayed.

GCCGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGGACGACA  >  NC_002947/1704529‑1704825

gCCGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCt                                                                                                                                                     >  2:395697/1‑150 (MQ=255)

  cGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGGGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTg                                                                                                                                                   >  2:1898162/1‑150 (MQ=255)

   ggaggaCAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGc                                                                                                                                                  <  1:1593760/150‑1 (MQ=255)

    gaggaCAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGcc                                                                                                                                                 >  1:937245/1‑150 (MQ=255)

    gaggaCAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGcc                                                                                                                                                 >  2:1846782/1‑150 (MQ=255)

         cAACGGCGACGGCGTGCTGATCGACAGCCCGGGGGCGCGGGGTCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTGCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGa                                                                                                                                            <  1:517155/150‑1 (MQ=255)

          aaCGGGGAAGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCCGCCATCTCCGACCTGTTCGTCGAAGTGGCGCGGTGCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCAAGCCCGCGaa                                                                                                                                           <  1:776011/150‑1 (MQ=255)

          aaCGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCAAGCCCGCGaa                                                                                                                                           <  2:2348923/150‑1 (MQ=255)

            cggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAaga                                                                                                                                         >  2:105847/1‑150 (MQ=255)

            cggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAaga                                                                                                                                         <  1:1844409/150‑1 (MQ=255)

            cggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAaga                                                                                                                                         <  1:1653243/150‑1 (MQ=255)

            cggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAaga                                                                                                                                         <  2:2292938/150‑1 (MQ=255)

             ggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCGAGCCCGCGAagac                                                                                                                                        <  1:165533/150‑2 (MQ=255)

             ggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCCTCTTCGCGGGCAAGCCCGCGAagac                                                                                                                                        <  1:316133/150‑2 (MQ=255)

               cgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGc                                                                                                                                      <  2:924838/150‑1 (MQ=255)

               cgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGc                                                                                                                                      >  2:725388/1‑150 (MQ=255)

                       tGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAg                                                                                                                              <  1:2211797/150‑1 (MQ=255)

                        gCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAgg                                                                                                                             >  2:218753/1‑150 (MQ=255)

                           gATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGttt                                                                                                                          <  2:2333815/150‑1 (MQ=255)

                            aTCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGACGGTACAGGTTTc                                                                                                                         <  2:407170/150‑1 (MQ=255)

                              cGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCgt                                                                                                                       <  2:1381083/150‑1 (MQ=255)

                              cGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCgt                                                                                                                       >  1:1381083/1‑150 (MQ=255)

                               gACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCgtg                                                                                                                      <  2:451481/150‑1 (MQ=255)

                               gACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCAAGCCCGCGa                                                                                                                                            <  1:1484317/128‑1 (MQ=255)

                               gACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCAAGCCCGCGa                                                                                                                                            >  2:1484317/1‑128 (MQ=255)

                                aCAGCCCGGCGGCGCGGGTGTTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCgtgt                                                                                                                     <  2:1099224/150‑1 (MQ=255)

                                  aGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAGGAGGCCGGTACAGGTTTCGTGTAc                                                                                                                   <  1:258995/150‑1 (MQ=255)

                                         cggcgCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAg                                                                                                            <  1:1415495/150‑1 (MQ=255)

                                           gcgtGGGTTCTAGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTTGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGAACAGGTTTCGTGTACTAGAAAGGa                                                                                                          <  1:646614/150‑1 (MQ=255)

                                                  tGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCg                                                                                                   <  1:1846782/150‑1 (MQ=255)

                                                     tGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTg                                                                                                <  1:1962220/150‑1 (MQ=255)

                                                      gtccTACCTGGACGACCCGGCCAACTCCGATCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTgg                                                                                               <  1:99076/148‑1 (MQ=255)

                                                      ggCCTACCGGGACGCCCCGGCCAACTCCGACCTGTTCGGCGAAGGGGCGCGGTGCCAGCGCTCGGGCAATTGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCGGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTgg                                                                                               <  2:795027/150‑1 (MQ=255)

                                                       gCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGGAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGa                                                                                              <  2:514822/150‑1 (MQ=255)

                                                       gCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGa                                                                                              <  1:1456213/150‑1 (MQ=255)

                                                         cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGGGTTCCCGGTGGACg                                                                                            >  1:250552/1‑150 (MQ=255)

                                                          tACCTGGACGACCCGGGCAACTCCGACATGCTCGTCGATGTGGCTCGGTTTCATCGCTCGGTCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGa                                                                                           >  2:636533/1‑150 (MQ=255)

                                                            ccTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAt                                                                                         <  1:1484489/150‑1 (MQ=255)

                                                            ccTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAt                                                                                         >  1:1460383/1‑150 (MQ=255)

                                                            ccTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCAAGCCCGCGAaga                                                                                                                                         >  2:279774/1‑102 (MQ=255)

                                                            ccTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCAAGCCCGCGAaga                                                                                                                                         <  1:279774/102‑1 (MQ=255)

                                                             cTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTAGAGGGTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATa                                                                                        >  1:635149/1‑150 (MQ=255)

                                                             cTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAg                                                                                                            <  1:348249/130‑1 (MQ=255)

                                                             cTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATa                                                                                        >  2:1826353/1‑150 (MQ=255)

                                                               ggACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATAcc                                                                                      >  2:678598/1‑150 (MQ=255)

                                                                     cccGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCaaa                                                                                <  2:1216229/150‑1 (MQ=255)

                                                                     catggCCAATTCTGAGCTGTTCGTCGTAGCTGCGCGGTCCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGCCTTCCCCGCGAAGAGGCCGGTACAGGTTTCGGGTACTAGAAAGGAGTTCCCGGTGGAGGAATACCAGCaaa                                                                                <  2:137212/147‑1 (MQ=255)

                                                                      ccGGCCAACTCCGACCTGGTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAAc                                                                               >  2:1299547/1‑150 (MQ=255)

                                                                        ggCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATAACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGAAGTTCCCGGTGGACGAATACCAGCAAACCa                                                                             >  2:258104/1‑150 (MQ=255)

                                                                          ccAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATc                                                                           <  2:1550953/150‑1 (MQ=255)

                                                                           cAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTccc                                                                                                    >  2:559001/1‑124 (MQ=255)

                                                                           cAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTccc                                                                                                    <  1:559001/124‑1 (MQ=255)

                                                                             aCTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCcgc                                                                        >  2:2185919/1‑150 (MQ=255)

                                                                               tCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCgcgc                                                                      >  1:195723/1‑150 (MQ=255)

                                                                               tCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCgcgc                                                                      >  1:434326/1‑150 (MQ=255)

                                                                                 cGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCCTCTTCGCGGGCAAGCCCGCGAagac                                                                                                                                        >  2:2090495/1‑81 (MQ=37)

                                                                                 cGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCCTCTTCGCGGGCAAGCCCGCGAagac                                                                                                                                        <  1:2090495/82‑2 (MQ=37)

                                                                                  gACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCt                                                                   <  1:1636188/150‑1 (MQ=255)

                                                                                    ccTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGt                                                                 <  1:1826353/150‑1 (MQ=255)

                                                                                     cTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTc                                                                >  2:1520713/1‑150 (MQ=255)

                                                                                        ttCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTATAAAGGAGTTCCCGGTGGACGAATCAAACGAAACCATCCGCGCCCTGTCCGa                                                             >  1:611275/1‑150 (MQ=255)

                                                                                         tcgtcgAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGAc                                                            <  1:1898162/150‑1 (MQ=255)

                                                                                           gtcgAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCg                                                          >  2:776087/1‑150 (MQ=255)

                                                                                              gAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGc                                                                                                                                                  <  1:1816250/59‑1 (MQ=255)

                                                                                                    gcgcGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCcgc                                                                        <  1:57213/127‑1 (MQ=255)

                                                                                                    gcgcGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCcgc                                                                        >  2:57213/1‑127 (MQ=255)

                                                                                                    gcgcGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTcgc                                                 <  1:2185919/150‑1 (MQ=255)

                                                                                                     cgcgGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCGTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTcgcg                                                <  1:637733/150‑1 (MQ=255)

                                                                                                            ccAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCTGca                                             >  2:2129719/1‑146 (MQ=255)

                                                                                                            ccAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCTGca                                             <  1:2129719/146‑1 (MQ=255)

                                                                                                              accgcTCGGCCACATCACCGTGCTCGCGTATTCGCGGGAGTGCCCGGATATAGCCCGGTACAGGGTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACg                                       <  2:635149/148‑1 (MQ=255)

                                                                                                              aGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTAc                                                                                                                   >  2:193342/1‑74 (MQ=255)

                                                                                                               gcgcTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGc                                      <  1:2219342/150‑1 (MQ=255)

                                                                                                                 gcTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCg                                    >  2:2343906/1‑150 (MQ=255)

                                                                                                                       gCAAATGACCGTTTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCgg                              <  2:1924581/150‑1 (MQ=255)

                                                                                                                       gCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGTTTTCGTGTACTAGAAAGAAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGa                                   <  1:2202564/145‑1 (MQ=255)

                                                                                                                       gCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCgg                              <  2:2096193/150‑1 (MQ=255)

                                                                                                                       gCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCgg                              <  1:1520713/150‑1 (MQ=255)

                                                                                                                       gCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCgg                              <  1:776087/150‑1 (MQ=255)

                                                                                                                       gCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGAAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGa                                   >  2:2202564/1‑145 (MQ=255)

                                                                                                                             gACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCtt                        <  1:105847/150‑1 (MQ=255)

                                                                                                                              aCCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTg                       <  2:2051050/150‑1 (MQ=255)

                                                                                                                                 gtgtTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGAcg                    <  1:560264/150‑1 (MQ=255)

                                                                                                                                     cggcctCTTCGCGGGCAAGCCCGCGAAGACGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGc                                      >  1:2096692/6‑128 (MQ=39)

                                                                                                                                     cggCCTCTTCGCGGGCAAGCCCGCGAAGACGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGc                                      <  2:2096692/127‑1 (MQ=39)

                                                                                                                                      ggCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTg               >  1:823374/1‑150 (MQ=255)

                                                                                                                                       gCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATcc                                                                          >  1:1053264/1‑90 (MQ=255)

                                                                                                                                       gCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATcc                                                                          <  2:1053264/90‑1 (MQ=255)

                                                                                                                                       gCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCgg                              >  2:569131/1‑134 (MQ=255)

                                                                                                                                       gCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTACCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAAACGCCGCTCCGGGTCCATGAcgctgcga              >  2:138800/1‑145 (MQ=255)

                                                                                                                                        cGTCTTCGCGGGCTCGTCCGCGAAGAGGCCGGTACCGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGaa             <  2:250552/150‑1 (MQ=255)

                                                                                                                                          tCTTCGCGGGCAAGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGt           >  1:1697297/1‑150 (MQ=255)

                                                                                                                                            ttCGCGTGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTgg         <  1:725388/150‑1 (MQ=255)

                                                                                                                                            ttCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTgg         >  2:1459205/1‑150 (MQ=255)

                                                                                                                                            ttCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTgg         <  1:452259/150‑1 (MQ=255)

                                                                                                                                             tCGCTGTCTTGCCTGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTggg        <  2:611275/150‑1 (MQ=255)

                                                                                                                                               gcgGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGc                                      >  1:1008121/1‑118 (MQ=255)

                                                                                                                                               gcgGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGc                                      <  2:1008121/118‑1 (MQ=255)

                                                                                                                                               gcgGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGgac      <  2:1460383/150‑1 (MQ=255)

                                                                                                                                                   gCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGGACGaca  >  1:2259890/1‑150 (MQ=255)

GCCGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGGACGACA  >  NC_002947/1704529‑1704825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`