BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,704,679	T→A	12.7%	M1* (TTG→TAG) †‡	PP_1499 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,704,679	0	T	A	12.7%	309.5 / 32.3	119	M1* (TTG→TAG) †‡	PP_1499	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (42/61); new base A (6/9); total (48/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

Only 100 of 119 total aligned reads displayed.

CGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGGACGACA  >  NC_002947/1704531‑1704825

cGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGGGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTg                                                                                                                                                   >  2:1898162/1‑150 (MQ=255)

  gaggaCAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGcc                                                                                                                                                 >  1:937245/1‑150 (MQ=255)

  gaggaCAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGcc                                                                                                                                                 >  2:1846782/1‑150 (MQ=255)

  gaggaCAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGcc                                                                                                                                                 >  1:1550953/1‑150 (MQ=255)

       cAACGGCGACGGCGTGCTGATCGACAGCCCGGGGGCGCGGGGTCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTGCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGa                                                                                                                                            <  1:517155/150‑1 (MQ=255)

        aaCGGGGAAGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCCGCCATCTCCGACCTGTTCGTCGAAGTGGCGCGGTGCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCAAGCCCGCGaa                                                                                                                                           <  1:776011/150‑1 (MQ=255)

        aaCGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCAAGCCCGCGaa                                                                                                                                           <  2:2348923/150‑1 (MQ=255)

          cggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAaga                                                                                                                                         >  2:105847/1‑150 (MQ=255)

          cggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAaga                                                                                                                                         <  1:1844409/150‑1 (MQ=255)

          cggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAaga                                                                                                                                         <  1:1653243/150‑1 (MQ=255)

           ggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCGAGCCCGCGAagac                                                                                                                                        <  1:165533/150‑2 (MQ=255)

           ggcgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCCTCTTCGCGGGCAAGCCCGCGAagac                                                                                                                                        <  1:316133/150‑2 (MQ=255)

             cgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGc                                                                                                                                      <  2:924838/150‑1 (MQ=255)

             cgacggcgTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGc                                                                                                                                      >  2:725388/1‑150 (MQ=255)

                     tGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAg                                                                                                                              <  1:2211797/150‑1 (MQ=255)

                      gCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAgg                                                                                                                             >  2:218753/1‑150 (MQ=255)

                         gATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGc                                                                                                                                      <  2:1428934/138‑1 (MQ=255)

                         gATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGc                                                                                                                                      >  1:1428934/1‑138 (MQ=255)

                         gATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGttt                                                                                                                          <  2:2333815/150‑1 (MQ=255)

                          aTCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGACGGTACAGGTTTc                                                                                                                         <  2:407170/150‑1 (MQ=255)

                            cGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCgt                                                                                                                       <  2:1381083/150‑1 (MQ=255)

                            cGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCgt                                                                                                                       >  1:1381083/1‑150 (MQ=255)

                             gACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCgtg                                                                                                                      <  2:451481/150‑1 (MQ=255)

                             gACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCAAGCCCGCGa                                                                                                                                            <  1:1484317/128‑1 (MQ=255)

                             gACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCAAGCCCGCGa                                                                                                                                            >  2:1484317/1‑128 (MQ=255)

                              aCAGCCCGGCGGCGCGGGTGTTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCgtgt                                                                                                                     <  2:1099224/150‑1 (MQ=255)

                              aCAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCgtgt                                                                                                                     >  1:956360/1‑150 (MQ=255)

                                       cggcgCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAg                                                                                                            <  1:1415495/150‑1 (MQ=255)

                                         gcgtGGGTTCTAGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTTGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGAACAGGTTTCGTGTACTAGAAAGGa                                                                                                          <  1:646614/150‑1 (MQ=255)

                                                tGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCg                                                                                                   <  1:1846782/150‑1 (MQ=255)

                                                   tGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTGGTCTAAGTGGTGCGGTTCCAGCTCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTg                                                                                                <  2:1172081/150‑1 (MQ=255)

                                                    gtccTACCTGGACGACCCGGCCAACTCCGATCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTgg                                                                                               <  1:99076/148‑1 (MQ=255)

                                                    ggCCTACCGGGACGCCCCGGCCAACTCCGACCTGTTCGGCGAAGGGGCGCGGTGCCAGCGCTCGGGCAATTGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCGGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTgg                                                                                               <  2:795027/150‑1 (MQ=255)

                                                     gCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGGAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGa                                                                                              <  2:514822/150‑1 (MQ=255)

                                                       cTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGGGTTCCCGGTGGACg                                                                                            >  1:250552/1‑150 (MQ=255)

                                                        tACCTGGACGACCCGGGCAACTCCGACATGCTCGTCGATGTGGCTCGGTTTCATCGCTCGGTCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGa                                                                                           >  2:636533/1‑150 (MQ=255)

                                                          ccTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAt                                                                                         <  1:1484489/150‑1 (MQ=255)

                                                          ccTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAAt                                                                                         >  1:1460383/1‑150 (MQ=255)

                                                          ccTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCAAGCCCGCGAaga                                                                                                                                         <  1:279774/102‑1 (MQ=255)

                                                           cTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAg                                                                                                            >  2:348249/1‑130 (MQ=255)

                                                           cTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAg                                                                                                            <  1:348249/130‑1 (MQ=255)

                                                           cTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATa                                                                                        >  2:1826353/1‑150 (MQ=255)

                                                             ggACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCgt                                                                                                                       <  2:2162203/117‑1 (MQ=255)

                                                             ggACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCgt                                                                                                                       >  1:2162203/1‑117 (MQ=255)

                                                             ggACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATAcc                                                                                      >  2:678598/1‑150 (MQ=255)

                                                              gacgacCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCa                                                                                     <  1:395697/150‑1 (MQ=255)

                                                                   cccGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCaaa                                                                                <  2:1216229/150‑1 (MQ=255)

                                                                    ccGGCCAACTCCGACCTGGTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAAc                                                                               >  2:1299547/1‑150 (MQ=255)

                                                                      ggCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATAACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGAAGTTCCCGGTGGACGAATACCAGCAAACCa                                                                             >  2:258104/1‑150 (MQ=255)

                                                                        ccAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATc                                                                           <  2:1550953/150‑1 (MQ=255)

                                                                         cAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTccc                                                                                                    <  1:559001/124‑1 (MQ=255)

                                                                         cAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTccc                                                                                                    >  2:559001/1‑124 (MQ=255)

                                                                           aCTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCcgc                                                                        >  2:2185919/1‑150 (MQ=255)

                                                                             tCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCgcgc                                                                      >  1:195723/1‑150 (MQ=255)

                                                                             tCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCgcgc                                                                      >  1:434326/1‑150 (MQ=255)

                                                                             tCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCCTCTTCGCGGGCAAGCCCGCGaa                                                                                                                                           <  1:670509/81‑1 (MQ=38)

                                                                               cGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCCTCTTCGCGGGCAAGCCCGCGAagac                                                                                                                                        <  1:2090495/82‑2 (MQ=37)

                                                                               cGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCCTCTTCGCGGGCAAGCCCGCGAagac                                                                                                                                        >  2:2090495/1‑81 (MQ=37)

                                                                                gACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCt                                                                   <  1:1636188/150‑1 (MQ=255)

                                                                                  ccTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGt                                                                 <  1:1826353/150‑1 (MQ=255)

                                                                                      ttCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTATAAAGGAGTTCCCGGTGGACGAATCAAACGAAACCATCCGCGCCCTGTCCGa                                                             >  1:611275/1‑150 (MQ=255)

                                                                                       tcgtcgAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGAc                                                            <  1:1898162/150‑1 (MQ=255)

                                                                                         gtcgAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCg                                                          >  2:776087/1‑150 (MQ=255)

                                                                                            gAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGc                                                                                                                                                  >  2:1816250/1‑59 (MQ=255)

                                                                                                  gcgcGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCcgc                                                                        <  1:57213/127‑1 (MQ=255)

                                                                                                  gcgcGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCcgc                                                                        >  2:57213/1‑127 (MQ=255)

                                                                                                  gcgcGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTcgc                                                 <  1:2185919/150‑1 (MQ=255)

                                                                                                  gcgcGGTTCCAGCGATCGGGCAGATGCTCGTGTTGGCGTCTGCGGGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAAGCCTCCGCGCCGTGTCCGACCGCATCGTcgc                                                 <  1:1799182/150‑1 (MQ=255)

                                                                                                   cgcgGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCGTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTcgcg                                                <  1:637733/150‑1 (MQ=255)

                                                                                                    gcgGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCgg                                               <  2:195723/150‑1 (MQ=255)

                                                                                                          ccAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCTGca                                             <  1:2129719/146‑1 (MQ=255)

                                                                                                          ccAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCTGca                                             >  2:2129719/1‑146 (MQ=255)

                                                                                                            accgcTCGGCCACATCACCGTGCTCGCGTATTCGCGGGAGTGCCCGGATATAGCCCGGTACAGGGTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACg                                       <  2:635149/148‑1 (MQ=255)

                                                                                                            aGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTAc                                                                                                                   <  1:193342/74‑1 (MQ=255)

                                                                                                            aGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTAc                                                                                                                   >  2:193342/1‑74 (MQ=255)

                                                                                                             gcgcTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGc                                      <  1:2219342/150‑1 (MQ=255)

                                                                                                               gcTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCg                                    >  2:2343906/1‑150 (MQ=255)

                                                                                                                    ggCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCg                               >  1:2335115/1‑150 (MQ=255)

                                                                                                                     gCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCgg                              <  1:1520713/150‑1 (MQ=255)

                                                                                                                     gCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCgg                              <  2:2096193/150‑1 (MQ=255)

                                                                                                                     gCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGAAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGa                                   >  2:2202564/1‑145 (MQ=255)

                                                                                                                           gACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCtt                        <  1:105847/150‑1 (MQ=255)

                                                                                                                            aCCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTg                       <  2:2051050/150‑1 (MQ=255)

                                                                                                                               gtgtTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGAcg                    <  1:560264/150‑1 (MQ=255)

                                                                                                                                   cggcctCTTCGCGGGCAAGCCCGCGAAGACGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGc                                      >  1:2096692/6‑128 (MQ=39)

                                                                                                                                    ggCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTg               >  1:823374/1‑150 (MQ=255)

                                                                                                                                     gCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATcc                                                                          >  1:1053264/1‑90 (MQ=255)

                                                                                                                                     gCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCgg                              >  2:569131/1‑134 (MQ=255)

                                                                                                                                     gCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCgg                              <  1:569131/134‑1 (MQ=255)

                                                                                                                                     gCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTACCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAAACGCCGCTCCGGGTCCATGAcgctgcga              >  2:138800/1‑145 (MQ=255)

                                                                                                                                      cGTCTTCGCGGGCTCGTCCGCGAAGAGGCCGGTACCGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGaa             <  2:250552/150‑1 (MQ=255)

                                                                                                                                        tCTTCGCGGGCAAGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGt           >  1:1697297/1‑150 (MQ=255)

                                                                                                                                          ttCGCGTGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTgg         <  1:725388/150‑1 (MQ=255)

                                                                                                                                          ttCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTgg         >  2:1459205/1‑150 (MQ=255)

                                                                                                                                           tCGCTGTCTTGCCTGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTggg        <  2:611275/150‑1 (MQ=255)

                                                                                                                                             gcgGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGc                                      <  2:1008121/118‑1 (MQ=255)

                                                                                                                                             gcgGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGc                                      >  1:1008121/1‑118 (MQ=255)

                                                                                                                                             gcgGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGgac      <  2:1460383/150‑1 (MQ=255)

                                                                                                                                                 gCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGGACGaca  >  1:2259890/1‑150 (MQ=255)

                                                                                                                                                 gCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGGACGaca  <  2:2335115/150‑1 (MQ=255)

CGGAGGACAACGGCGACGGCGTGCTGATCGACAGCCCGGCGGCGCGGGTGCTGGCCTACCTGGACGACCCGGCCAACTCCGACCTGTTCGTCGAAGTGGCGCGGTTCCAGCGCTCGGGCAAATGACCGTGTTGGCGTCTTCGCGGGCTTGCCCGCGAAGAGGCCGGTACAGGTTTCGTGTACTAGAAAGGAGTTCCCGGTGGACGAATACCAGCAAACCATCCGCGCCCTGTCCGACCGCATCGTCGCGGCACAGACGCCGATCCGGGTCCTTGACGCGGTGAAGTGGGACGACA  >  NC_002947/1704531‑1704825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`