Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,713,557 A→C 5.9% A153A (GCA→GCC)  tsaB → endopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,713,5570AC 5.9% 259.3 / 6.4 85A153A (GCA→GCC) tsaBendopeptidase
Reads supporting (aligned to +/- strand):  ref base A (30/50);  new base C (2/3);  total (32/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01

TTGCGCGAGCATGGCGTGCAGCAGGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGA  >  NC_002947/1713408‑1713703
                                                                                                                                                     |                                                                                                                                                  
ttGCGCGAGCATGGCGTGCAGCAGGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAGAGTGTAAGGACACCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCggca                                                                                                                                                    >  2:1268953/1‑150 (MQ=255)
  gcgcGAGCATGGCGTGCAGCAGGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCggcagg                                                                                                                                                  <  1:2155352/150‑1 (MQ=255)
     tgAGCATGGCGGGCAGCCGGTGGCCGTTGCTATCAATGCGCGCATGGATGAGGTTTACTGGGGCTGTTACCAGGCCATAGCGGGCGAAATGCGCCTGTTGGGCTGTGAAGCGGTGTTGCCGCCCGAGCAGTTGGCGCTGCCGgaaggcag                                                                                                                                               <  2:450689/149‑1 (MQ=255)
      gAGCATGGCGTGCAGCAGGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGgcagc                                                                                                                                              >  2:1409896/1‑150 (MQ=255)
      gAGCAGGGCGTGCAGCAGGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCTGCCGgcagc                                                                                                                                              <  1:93414/150‑1 (MQ=255)
           tGGCGTGCAGCAGGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATgg                                                                                                                                         <  1:1238562/150‑1 (MQ=255)
            ggCGTGCAGCAGGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCTGCCGgcagcgccacc                                                                                                                                        <  1:676852/150‑7 (MQ=255)
            ggCGTGCAGCAGGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGc                                                                                                                                        <  2:1030033/150‑1 (MQ=255)
              cGTGCAGCAGGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGa                                                                                                                                      <  2:460979/150‑1 (MQ=255)
               gTGCAGCAGGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCACCAATgcccag                                                                                                                                     <  2:32665/150‑6 (MQ=255)
                 gcagcaGGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTg                                                                                                                                   <  2:2067869/150‑1 (MQ=255)
                 gcagcaGGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTg                                                                                                                                   <  1:754220/150‑1 (MQ=255)
                   agcagGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGt                                                                                                                                 <  2:1446921/150‑1 (MQ=255)
                   agcagGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGt                                                                                                                                 >  1:1428001/1‑150 (MQ=255)
                      gggTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCg                                                                                                                              <  2:1800856/149‑1 (MQ=255)
                        ctgaccGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGGCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGa                                                                                                                                      >  2:1399665/5‑140 (MQ=255)
                        ctgaccGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGGCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGa                                                                                                                                      <  1:1399665/136‑1 (MQ=255)
                          ggCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGc                                                                                                                          <  2:246738/150‑1 (MQ=255)
                           gCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGcc                                                                                                                         <  1:800538/150‑1 (MQ=255)
                           gCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGCGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGcc                                                                                                                         <  1:2294319/150‑1 (MQ=255)
                              gCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGt                                                                                                                      >  2:1162523/1‑150 (MQ=255)
                                tGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTAc                                                                                                                    <  1:1409896/150‑1 (MQ=255)
                                tGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTAc                                                                                                                    <  2:1285035/150‑1 (MQ=255)
                                  ccATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCg                                                                                                                  >  2:1767125/1‑150 (MQ=255)
                                   cATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCgg                                                                                                                 <  2:1428001/150‑1 (MQ=255)
                                    aTCGATGCGCGCGTGGATAAAGACTACTGGGGATGCGACCAGGCCACAGCGGACGAAATACGACTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGAGGGTCGGTGCCGGTACCggg                                                                                                                >  2:70022/1‑150 (MQ=255)
                                    aTCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCggg                                                                                                                >  2:2128536/1‑150 (MQ=255)
                                      cGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTg                                                                                                              <  1:21286/150‑1 (MQ=255)
                                       gATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTgg                                                                                                             <  1:1165223/150‑1 (MQ=255)
                                       gATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTgg                                                                                                             <  1:2321821/150‑1 (MQ=255)
                                               cATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGc                                                                                                     <  2:1934866/150‑1 (MQ=255)
                                                  ggATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGAAATGGCGAGTGGTTCGGTGCCGGTTCCTGGTGGGGCTATGCCgc                                                                                                  <  2:594003/150‑2 (MQ=255)
                                                    aTGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGGTCGGTGCCGGTACCGGGTGGGGCTATGTCGCTc                                                                                                >  2:509851/1‑150 (MQ=255)
                                                      gAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGc                                                                                              >  1:769429/1‑150 (MQ=255)
                                                          tttACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGTGCTGCCGGCAGGCAGAAATGGCGAGTGGTTCGGTGCCGGTACCGGGGGGGGCTATGCCGATCGCCTgg                                                                                          >  2:1387531/1‑150 (MQ=255)
                                                            tACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCg                                                                                        <  2:769429/150‑1 (MQ=255)
                                                              cTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGt                                                                                      >  1:512160/1‑150 (MQ=255)
                                                               tGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCTGCCGgcagcgcca                                                                                                                                          <  2:2018015/97‑5 (MQ=255)
                                                               tGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCTGCCGgcagcgcca                                                                                                                                          >  1:2018015/1‑93 (MQ=255)
                                                               tGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTa                                                                                     <  2:2148563/150‑1 (MQ=255)
                                                                 gggCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACa                                                                                   <  1:2128536/150‑1 (MQ=255)
                                                                 gggCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGCGGCACGTCCGGCAGGCAGCAATGGCGAGAGTTGCGGATCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACa                                                                                   >  1:1740559/1‑150 (MQ=255)
                                                                  ggCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAg                                                                                  <  2:1937684/150‑1 (MQ=255)
                                                                   gctgctACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTCCAgg                                                                                 >  1:228936/1‑150 (MQ=255)
                                                                   gctgctACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAgg                                                                                 >  2:1330002/1‑150 (MQ=255)
                                                                         aCCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTa                                                                                                        <  2:812425/121‑1 (MQ=255)
                                                                         aCCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTa                                                                                                        >  1:812425/1‑121 (MQ=255)
                                                                          ccAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCgg                                                                                                                 >  2:1249970/1‑111 (MQ=255)
                                                                          ccAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCgg                                                                                                                 <  1:1249970/111‑1 (MQ=255)
                                                                           cAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAgcggcg                                                                         >  2:548481/1‑150 (MQ=255)
                                                                            aGGNCACAGCGGGCGAAATCAGCCGTGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGNANCGGGTGGGGCTATGCNGATCGCCTGGCGGTACAGGTAGCGGCGa                                                                        <  1:1330002/150‑1 (MQ=255)
                                                                            aGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGa                                                                        <  2:2005346/150‑1 (MQ=255)
                                                                            aGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTAAGCCGAGCGCCTGGCGGTACAGGTAGCGGCGa                                                                        <  1:1268953/150‑1 (MQ=255)
                                                                             ggCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTCACGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAg                                                                       <  1:305557/150‑1 (MQ=255)
                                                                                cacaGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCAGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCaa                                                                    <  1:1266564/150‑1 (MQ=255)
                                                                                       ggCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCt                                                             <  1:182064/150‑1 (MQ=255)
                                                                                          gAAATGCACCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGcc                                                          >  1:1100603/1‑150 (MQ=255)
                                                                                           aaaTGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGCTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCt                                                         <  2:1740559/150‑1 (MQ=255)
                                                                                                  ccTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCaa                                                  <  2:2218197/150‑1 (MQ=255)
                                                                                                   cTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAAt                                                 >  1:589613/1‑150 (MQ=255)
                                                                                                    tGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATg                                                <  2:2306797/150‑1 (MQ=255)
                                                                                                        cGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGctct                                            <  1:724311/150‑1 (MQ=255)
                                                                                                         ggCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTg                                           >  1:1192969/1‑150 (MQ=255)
                                                                                                          gCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTgg                                          >  2:2106465/1‑150 (MQ=255)
                                                                                                           ccGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGa                                         <  1:1208056/150‑1 (MQ=255)
                                                                                                           ccGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGa                                         <  1:468191/150‑1 (MQ=255)
                                                                                                            cGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGAc                                        >  2:1771936/1‑150 (MQ=255)
                                                                                                                  gCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATtctc                                  >  2:770141/1‑150 (MQ=255)
                                                                                                                   ccGTGTTGCCGCCCGAGCAGGTGGCGCTGCCggca                                                                                                                                                    <  2:22973/35‑1 (MQ=255)
                                                                                                                   ccGTGTTGCCGCCCGAGCAGGTGGCGCTGCCggca                                                                                                                                                    >  1:22973/1‑35 (MQ=255)
                                                                                                                     gtgtTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGc                               <  1:1162523/150‑1 (MQ=255)
                                                                                                                            cgccCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTAc                                                                                    <  1:1609971/90‑1 (MQ=255)
                                                                                                                            cgccCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTAc                                                                                    >  2:1609971/1‑90 (MQ=255)
                                                                                                                             gccCGAGCAGGTGGCGCTGCCGGCAGGCAGGAATGGCGGGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCttggccc                       <  2:88217/150‑7 (MQ=255)
                                                                                                                               ccGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCt                     >  1:72470/1‑150 (MQ=255)
                                                                                                                                cGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCtt                    >  1:1006288/1‑150 (MQ=255)
                                                                                                                                 gAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCttt                   >  1:710427/1‑150 (MQ=255)
                                                                                                                                   gCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGc                 <  1:1767125/150‑1 (MQ=255)
                                                                                                                                   gCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGc                 <  1:204039/150‑1 (MQ=255)
                                                                                                                                     aGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCt               <  2:512160/150‑1 (MQ=255)
                                                                                                                                       gTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTgg                                                                                          >  2:954548/1‑73 (MQ=255)
                                                                                                                                       gTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTgg                                                                                          <  1:954548/73‑1 (MQ=255)
                                                                                                                                       gTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCTGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTgg             <  1:770141/150‑1 (MQ=255)
                                                                                                                                       gTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTgg             <  1:2106465/150‑1 (MQ=255)
                                                                                                                                         attgcTGCCTGCCGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGc           >  1:2180551/4‑150 (MQ=255)
                                                                                                                                             cTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACa                                       <  2:1763908/118‑1 (MQ=255)
                                                                                                                                             cTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACa                                       >  1:1763908/1‑118 (MQ=255)
                                                                                                                                               gCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTgg     <  2:72470/150‑1 (MQ=255)
                                                                                                                                                ccGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGc    <  1:854204/150‑1 (MQ=255)
                                                                                                                                                ccGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGc    >  1:1286010/1‑150 (MQ=255)
                                                                                                                                                  ggcaggcaGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTTGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGATTATTTCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTcgcgc  <  2:369796/150‑2 (MQ=255)
                                                                                                                                                  ggcaggcaGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGa  <  2:1100603/150‑1 (MQ=255)
                                                                                                                                                   gcaggcagCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTg                                           >  2:1427386/1‑108 (MQ=255)
                                                                                                                                                   gcaggcagCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTg                                           <  1:1427386/108‑1 (MQ=255)
                                                                                                                                                     aggcagCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGctc                                             >  2:221525/1‑104 (MQ=255)
                                                                                                                                                     aggcagCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGctc                                             <  1:221525/104‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TTGCGCGAGCATGGCGTGCAGCAGGTGGCCGCTGCCATCGATGCGCGCATGGATGAGGTTTACTGGGGCTGCTACCAGGCCACAGCGGGCGAAATGCGCCTGGTCGGCCGTGAAGCCGTGTTGCCGCCCGAGCAGGTGGCGCTGCCGGCAGGCAGCAATGGCGAGTGGTTCGGTGCCGGTACCGGGTGGGGCTATGCCGATCGCCTGGCGGTACAGGTAGCGGCGAGCAACCCGGCTGCCTTGCCCAATGCTCTGGACATTCTCAGCCTGGCCACCTTTGCCTGGGCCCGTGGCGA  >  NC_002947/1713408‑1713703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑