Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,782,578 T→G 8.3% Q514P (CAG→CCG) ‡ glnD ← uridylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,782,5780TG 8.3% 261.8 / 15.8 97Q514P (CAG→CCG) ‡glnDuridylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (41/48);  new base G (4/4);  total (45/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.59e-01

CGGACAAGTCCTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAGGTTGAGGGT  >  NC_002947/1782432‑1782726
                                                                                                                                                  |                                                                                                                                                    
cGGACAAGTCCTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGc                                                                                                                                                   <  1:1025883/150‑1 (MQ=255)
cGGACAAGGCCGTTCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGc                                                                                                                                                   <  2:1978375/150‑1 (MQ=255)
 ggACAAGTCCTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCg                                                                                                                                                  >  1:1915257/1‑150 (MQ=255)
 ggACAAGTCCTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCg                                                                                                                                                  >  1:2290368/1‑150 (MQ=255)
     aaGTCCTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGAccc                                                                                                                                              >  1:134922/1‑150 (MQ=255)
     aaGTCCTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGAccc                                                                                                                                              >  1:2228374/1‑150 (MQ=255)
     aaGTCCTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCAGGGCGAcca                                                                                                                                              <  2:2162584/150‑2 (MQ=255)
       gTCCTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCtt                                                                                                                                            <  2:1051948/150‑1 (MQ=255)
        tCCTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGAccactc                                                                                                                                           <  2:1652623/150‑5 (MQ=255)
          cTTGCGCTGAGCGGTTGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGcc                                                                                                                                         <  2:919583/150‑1 (MQ=255)
          cTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGcc                                                                                                                                         >  2:1181025/1‑150 (MQ=255)
          cTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGcc                                                                                                                                         >  2:1222032/1‑150 (MQ=255)
          cTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGcc                                                                                                                                         <  2:1154408/150‑1 (MQ=255)
             gcgcTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGAt                                                                                                                                      <  2:2359321/150‑1 (MQ=255)
               gcTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGt                                                                                                                                    <  1:2004117/150‑1 (MQ=255)
                 tGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCGGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCAGGGCGAccactc                                                                                                                                           >  1:249987/1‑137 (MQ=255)
                 tGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCGGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCAGGGCGAccactc                                                                                                                                           <  2:249987/141‑5 (MQ=255)
                 tGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCg                                                                                                                                  >  1:364363/1‑150 (MQ=255)
                   aGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGCCCCTTGCCGATGTCGTg                                                                                                                                <  1:1091661/150‑1 (MQ=255)
                   aGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTg                                                                                                                                <  2:1896015/150‑1 (MQ=255)
                   aGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTg                                                                                                                                <  2:2290368/150‑1 (MQ=255)
                   aGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGCTGCCGTTCGCAGAACTTCTGCGTGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCTACCCTTGCCGATGTCGTg                                                                                                                                <  2:1081744/150‑1 (MQ=255)
                    gCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTgg                                                                                                                               <  2:2019251/150‑1 (MQ=255)
                       gtggtCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTAc                                                                                                                            <  1:1775279/150‑1 (MQ=255)
                        tggtCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACa                                                                                                                           >  2:103780/1‑150 (MQ=255)
                             gACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCg                                                                                                                      >  1:1335343/1‑150 (MQ=255)
                                  cACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAg                                                                                                                 <  2:512939/150‑1 (MQ=255)
                                  cACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAg                                                                                                                 >  2:1659216/1‑150 (MQ=255)
                                   aCCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAgg                                                                                                                >  2:129159/1‑150 (MQ=255)
                                   aCCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCAGGGCGAccac                                                                                                                                             >  1:1671091/1‑119 (MQ=255)
                                   aCCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCAGGGCGAccac                                                                                                                                             <  2:1671091/121‑3 (MQ=255)
                                     cAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTa                                                                                                              <  1:1659216/150‑1 (MQ=255)
                                     cAGGTGGGTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTa                                                                                                              >  1:824421/1‑150 (MQ=255)
                                       ggtggtTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGa                                                                                                            <  1:1662206/150‑1 (MQ=255)
                                        gtggtTCTGCACCAGCCAGACGATCAGTTGTCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGAt                                                                                                           <  1:1576811/150‑1 (MQ=255)
                                        gtggtTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGTAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGATTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGAt                                                                                                           <  1:435320/150‑1 (MQ=255)
                                             tCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCt                                                                                                      >  2:936971/1‑150 (MQ=255)
                                              cTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTc                                                                                                     <  2:824421/150‑1 (MQ=255)
                                                  aCCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGCCCAGGTAGATCAGCTCGGGt                                                                                                 >  2:53188/1‑150 (MQ=255)
                                                     agccagACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTg                                                                                              <  2:2228374/150‑1 (MQ=255)
                                                      gccagACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTgg                                                                                             >  1:1776684/1‑150 (MQ=255)
                                                         agACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCt                                                                                                                                                      >  1:1105161/1‑90 (MQ=255)
                                                         agACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCt                                                                                                                                                      <  2:1105161/90‑1 (MQ=255)
                                                               tCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGGTGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGcc                                                                                    <  2:1915257/150‑1 (MQ=255)
                                                                   gcggcTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCAt                                                                                >  2:1606856/1‑150 (MQ=255)
                                                                     ggcTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGa                                                                              <  2:543562/150‑1 (MQ=255)
                                                                     ggcTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGa                                                                              <  1:129159/150‑1 (MQ=255)
                                                                     ggcTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGa                                                                              <  2:419143/150‑1 (MQ=255)
                                                                      gcTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTgg                                                                                                                                                    <  1:1144183/79‑1 (MQ=255)
                                                                      gcTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTgg                                                                                                                                                    >  2:1144183/1‑79 (MQ=255)
                                                                      gcTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAg                                                                                         <  2:940859/138‑1 (MQ=255)
                                                                      gcTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAg                                                                                         >  1:940859/1‑138 (MQ=255)
                                                                         gTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAACCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCtt                                                                          <  2:2277146/150‑1 (MQ=255)
                                                                          tCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTgg                                                                                             >  2:584082/1‑130 (MQ=255)
                                                                          tCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTgg                                                                                             <  1:584082/130‑1 (MQ=255)
                                                                            ccAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTg                                                                                                                                <  1:1451924/93‑1 (MQ=255)
                                                                            ccAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTg                                                                                                                                >  2:1451924/1‑93 (MQ=255)
                                                                            ccAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCt                                                                       >  1:114387/1‑150 (MQ=255)
                                                                                    gCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGt                                                                                                 >  2:1179383/1‑116 (MQ=255)
                                                                                    gCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGt                                                                                                 <  1:1179383/116‑1 (MQ=255)
                                                                                      aaCTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCggg                                                             >  2:1087022/1‑150 (MQ=255)
                                                                                        cTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGaa                                                           <  1:470833/150‑1 (MQ=255)
                                                                                           gTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAAttt                                                        >  1:58259/1‑150 (MQ=255)
                                                                                           gTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGAAGATACCCGCGGGTGGTGGCAGGCTCGCCATGAGCTTGCTGGCCAGCGGGAAttt                                                        >  2:2202029/1‑150 (MQ=255)
                                                                                              ccGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTtctc                                                     >  2:408351/1‑150 (MQ=255)
                                                                                               cGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCg                                                    <  1:2086270/150‑1 (MQ=255)
                                                                                               cGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCg                                                    <  2:1776684/150‑1 (MQ=255)
                                                                                                gTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCgg                                                   >  2:1309344/1‑150 (MQ=255)
                                                                                                    gCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCg                                                    >  2:2375719/1‑145 (MQ=255)
                                                                                                    gCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCg                                                    <  1:2375719/145‑1 (MQ=255)
                                                                                                    gCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGacac                                               >  1:1632248/1‑150 (MQ=255)
                                                                                                    ccAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGacac                                               <  1:53188/149‑1 (MQ=255)
                                                                                                        aaCTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGc                                           <  1:2202029/150‑1 (MQ=255)
                                                                                                        aaCTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGc                                           <  2:364363/150‑1 (MQ=255)
                                                                                                           ttCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCgtg                                        >  2:897572/1‑150 (MQ=255)
                                                                                                              tGCGCGTCCACTGCACCGATTTCCGAGTGGTCGACCTGCCGCATCTTATGGATGGCGTGGTACAGCCCGGTGAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTAt                                     >  1:422442/1‑150 (MQ=255)
                                                                                                                  cgTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCa                                 <  1:1606856/150‑1 (MQ=255)
                                                                                                                  cgTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCa                                 <  1:1017527/150‑1 (MQ=255)
                                                                                                                  cgTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCa                                 <  2:1435922/150‑1 (MQ=255)
                                                                                                                   gTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCg                                                    <  1:2298517/130‑1 (MQ=255)
                                                                                                                   gTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCg                                                    >  2:2298517/1‑130 (MQ=255)
                                                                                                                      cACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGAGGGCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCtt                             <  1:463541/150‑1 (MQ=255)
                                                                                                                          gCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGCAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTgcgc                         >  2:619967/1‑150 (MQ=255)
                                                                                                                           cACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCa                        >  2:1759958/1‑150 (MQ=255)
                                                                                                                              cGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGCATTTGTCAGACACCGACGTGTAGTGCAGCCTGCGCAGGt                     >  1:1466962/1‑150 (MQ=255)
                                                                                                                                aTTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCg                                                    >  1:909442/1‑117 (MQ=255)
                                                                                                                                aTTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCg                                                    <  2:909442/117‑1 (MQ=255)
                                                                                                                                 tttCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCt                  <  2:1632248/150‑1 (MQ=255)
                                                                                                                                   tCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTtg                >  1:597698/1‑150 (MQ=255)
                                                                                                                                       aagggTCGCCAGGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCa                                 <  2:1088493/126‑1 (MQ=255)
                                                                                                                                       aagggTCGCCAGGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCa                                 >  1:1088493/4‑129 (MQ=255)
                                                                                                                                        gTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAg           <  2:58259/150‑1 (MQ=255)
                                                                                                                                        agggTCGCCAGGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGACGTGTATTGCAGCTTGCGCAGGTGCTTGATGAg           >  2:1295873/3‑150 (MQ=255)
                                                                                                                                           gTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCgg                                                              <  1:93144/96‑1 (MQ=255)
                                                                                                                                           gTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCgg                                                              >  2:93144/1‑96 (MQ=255)
                                                                                                                                           gTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAGGtt        >  1:918015/1‑150 (MQ=255)
                                                                                                                                           gTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAGGtt        >  2:1293670/1‑150 (MQ=255)
                                                                                                                                             cGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAGGTTGa      <  1:1087022/150‑1 (MQ=255)
                                                                                                                                                 cTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAGGTTGAGGGt  >  2:906970/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CGGACAAGTCCTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAGGTTGAGGGT  >  NC_002947/1782432‑1782726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑