Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,818,534 A→G 6.6% N212D (AAC→GAC)  PP_1622 → M23B subfamily metallopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,818,5340AG 6.6% 178.9 / 2.5 60N212D (AAC→GAC) PP_1622M23B subfamily metallopeptidase
Reads supporting (aligned to +/- strand):  ref base A (29/27);  new base G (2/2);  total (31/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01

TAAAGGCATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCTGCCAC  >  NC_002947/1818386‑1818681
                                                                                                                                                    |                                                                                                                                                   
tAAAGGCATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATaa                                                                                                                                                    <  2:1069839/150‑1 (MQ=255)
   aGGCATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCg                                                                                                                                                 >  2:1485702/1‑150 (MQ=255)
      cATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTGCGGTTATGACCGTAg                                                                                                                                              <  2:647665/150‑1 (MQ=255)
      cATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAg                                                                                                                                              >  2:1434230/1‑150 (MQ=255)
       aTTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTGCGGTTATGACCGTAgg                                                                                                                                             <  1:224349/150‑1 (MQ=255)
           atatCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGt                                                                                                                                         <  2:207592/150‑1 (MQ=255)
           atatCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGt                                                                                                                                         <  1:1568101/150‑1 (MQ=255)
             atCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTg                                                                                                                                       <  2:722835/150‑1 (MQ=255)
                 ccGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTc                                                                                                                                   <  1:2384566/150‑1 (MQ=255)
                   ggTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCgg                                                                                                                                 <  1:1485702/150‑1 (MQ=255)
                      gATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCGACGGTCATAACCGCAGGCTGTTGGTTCGGGAg                                                                                                                              <  1:1171243/150‑1 (MQ=255)
                           gggACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGgca                                                                                                                         >  2:2232241/1‑150 (MQ=255)
                                  ccTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTc                                                                                                                  >  1:451266/1‑150 (MQ=255)
                                   cTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATaa                                                                                                                                                    <  2:1533498/115‑1 (MQ=255)
                                   cTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATaa                                                                                                                                                    >  1:1533498/1‑115 (MQ=255)
                                   cTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCa                                                                                                                 >  2:402702/1‑150 (MQ=255)
                                          tgctgcGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGTTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAggcagg                                                                                                          <  2:881114/150‑1 (MQ=255)
                                          tgctgcGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAggcagg                                                                                                          <  1:2324939/150‑1 (MQ=255)
                                          tgctgcGTCTGATGGTGCGGTGGTTTATGACGGGAGTTGTGTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAggcagg                                                                                                          <  2:266175/150‑1 (MQ=255)
                                            ctgcGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAggcagggc                                                                                                        >  1:370263/1‑150 (MQ=255)
                                                   tGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCaa                                                                                                                <  1:2333113/135‑1 (MQ=255)
                                                   tGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCaa                                                                                                                >  2:2333113/1‑135 (MQ=255)
                                                         tGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGa                                                                                           >  2:1824208/1‑150 (MQ=255)
                                                               ggtTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATggg                                                                                     <  2:451266/150‑1 (MQ=255)
                                                                            aGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGcaca                                                                        <  1:1824208/150‑1 (MQ=255)
                                                                                gTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATc                                                                    >  2:1874767/1‑150 (MQ=255)
                                                                                 tttGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCg                                                                   >  1:230673/1‑150 (MQ=255)
                                                                                  ttGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCgg                                                                  <  1:1434230/150‑1 (MQ=255)
                                                                                      ggggCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCg                                                                   <  1:1348530/145‑1 (MQ=255)
                                                                                      ggggCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCg                                                                   >  2:1348530/1‑145 (MQ=255)
                                                                                       gggCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGTGTCTACGGGCACAGATCGGGTGaa                                                             <  1:1874767/150‑1 (MQ=255)
                                                                                          cgaCGGAGAGTGGATCCTCCTGAAGTAAAGCGTGACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCt                                                          <  1:125734/148‑1 (MQ=255)
                                                                                            aCGGCGAGCTGATCATCATCAAGCAAAGCGACACCTACGTCAGTGCCTACGGTCCTACCCGCAGGCTGATGGCGCGGGAGGGGCAGCAGGGCAGGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCAGAGATCGGGTGAAGCTGc                                                        >  1:736673/1‑150 (MQ=255)
                                                                                              ggCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGa                                                                                                                               <  2:1046950/77‑1 (MQ=255)
                                                                                              ggCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGa                                                                                                                               >  1:1046950/1‑77 (MQ=255)
                                                                                                      gATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGAt                                                                                                 <  1:1471400/99‑1 (MQ=255)
                                                                                                      gATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGAt                                                                                                 >  2:1471400/1‑99 (MQ=255)
                                                                                                       atcatcatcAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAAt                                                                                        <  2:978809/107‑1 (MQ=255)
                                                                                                       atcatcatcAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAAt                                                                                        >  1:978809/1‑107 (MQ=255)
                                                                                                       atcatcatcAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTAc                                                                               <  1:726075/116‑1 (MQ=255)
                                                                                                       atcatcatcAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTAc                                                                               >  2:726075/1‑116 (MQ=255)
                                                                                                          atcatcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTcgc                                          >  2:1115123/1‑150 (MQ=255)
                                                                                                             atcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGt                                                                                                                                         <  1:1818946/52‑1 (MQ=255)
                                                                                                             atcaAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGt                                                                                                                                         >  2:1818946/1‑52 (MQ=255)
                                                                                                                         gACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCACGGCAGGGTAGTCGATTGCTGAAATGGGGTCTACGGCCACAGATCAGTTGAAGCCGCATTTCGAGATTCGCCGCCAAGGCAAAccc                           >  2:451159/1‑150 (MQ=255)
                                                                                                                         gACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAAccc                           <  2:230673/150‑1 (MQ=255)
                                                                                                                              cTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGa                      >  2:438406/1‑150 (MQ=255)
                                                                                                                              cTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGAAGCAGGTCAAGGCAGGGCAGTCGATTg                                                                                               >  2:2044684/1‑77 (MQ=255)
                                                                                                                              cTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGAAGCAGGTCAAGGCAGGGCAGTCGATTg                                                                                               <  1:2044684/77‑1 (MQ=255)
                                                                                                                               tACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGACATTCGCCGCCAGGGCAAACCCGTCGAt                     >  2:533570/1‑150 (MQ=255)
                                                                                                                                   caacagCCTGCGGTTATGACCGTAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCAc                 >  2:1682578/6‑150 (MQ=255)
                                                                                                                                       tGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCgg                                                                  <  2:2283659/97‑1 (MQ=255)
                                                                                                                                       tGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCgg                                                                  >  1:2283659/1‑97 (MQ=255)
                                                                                                                                       tGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCa             >  1:467821/1‑150 (MQ=255)
                                                                                                                                        gCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAg            >  1:1849864/1‑150 (MQ=255)
                                                                                                                                         ccTGCGGTTATGACCGTAGGCTGTTGGTTCGGGAGGGGCANCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGt           >  1:1425759/1‑150 (MQ=255)
                                                                                                                                           tACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGt                         >  1:792158/1‑134 (MQ=255)
                                                                                                                                           tACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGt                         <  2:792158/134‑1 (MQ=255)
                                                                                                                                            aCGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAAc                             >  2:1281180/1‑129 (MQ=255)
                                                                                                                                            aCGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAAc                             <  1:1281180/129‑1 (MQ=255)
                                                                                                                                                tCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCTGcc    >  2:948441/1‑150 (MQ=255)
                                                                                                                                                 cATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCTGCCa   >  1:2361867/1‑150 (MQ=255)
                                                                                                                                                  aTAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCTGCCAc  <  2:1849864/150‑1 (MQ=255)
                                                                                                                                                    aaCCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCga                                                 <  2:2407017/101‑1 (MQ=255)
                                                                                                                                                    aaCCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCga                                                 >  1:2407017/1‑101 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TAAAGGCATTGATATCGCCGGTGATTTGGGACAGCCTGTTTTTGCTGCGTCTGATGGTGCGGTGGTTTATGCCGGGAGTGGTTTGAGGGGCTACGGCGAGCTGATCATCATCAAGCACAGCGACACCTACGTCAGTGCCTACGGTCATAACCGCAGGCTGTTGGTTCGGGAGGGGCAGCAGGTCAAGGCAGGGCAGTCGATTGCTGAAATGGGGTCTACGGGCACAGATCGGGTGAAGCTGCATTTCGAGATTCGCCGCCAGGGCAAACCCGTCGATCCACTCCAGTTCCTGCCAC  >  NC_002947/1818386‑1818681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑