| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 196,879 | G→A | 20.9% | V795V (GTG→GTA) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 196,879 | 0 | G | A | 20.9% | 57.8 / 12.5 | 35 | V795V (GTG→GTA) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base G (14/13); new base A (2/5); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.74e-01 | |||||||||||
ACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTC > NC_002947/196730‑196938 | aCCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTg > 2:60546/1‑150 (MQ=32) ccGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGa < 2:555110/150‑1 (MQ=32) gTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATc > 1:275403/1‑150 (MQ=32) gTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTAGATGCAGCCACCGCGACCCTGA‑C‑GGCAAGCCCGTCGGTCACCGAAGGTGACGTGATc > 2:2346479/1‑150 (MQ=11) cAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAc < 2:1794903/150‑1 (MQ=32) cGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTa > 2:919318/1‑150 (MQ=32) tCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTaca < 2:1262610/150‑1 (MQ=32) tCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGa < 2:57712/150‑1 (MQ=32) tCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGa > 1:2231537/1‑150 (MQ=32) gaACGCCACTGGCGGCAACTTCCAACAGCTGACTCCGAACCCGACGCCGGCTCAGAGCACGATCAACTACTCGGTCGCTGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCt < 2:756685/150‑1 (MQ=11) aaCGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGa < 1:1764600/145‑1 (MQ=32) aaCGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGa > 2:1764600/1‑145 (MQ=32) aCGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGa < 2:2231537/150‑1 (MQ=35) ggcggcAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCt < 1:919318/150‑1 (MQ=32) ggcggcAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCt < 2:2116040/150‑1 (MQ=32) ggcAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGcc > 1:1482414/1‑150 (MQ=32) gcAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGccc > 1:2060408/1‑150 (MQ=32) ttCGAACAGCTGTCCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCCGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGGCACCGAAGTCCGCTTGATCACCTACACCGTGACCCTGCGCAATCCTACCCAGAca > 2:973383/1‑149 (MQ=11) ttCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACg > 2:1965020/1‑150 (MQ=32) cGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGGCCCCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGcc > 2:373408/1‑150 (MQ=17) gAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGCGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCg < 2:2032269/150‑1 (MQ=17) aCAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGt > 1:2010230/1‑150 (MQ=32) cAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTg < 2:2234665/150‑1 (MQ=33) aGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGa > 1:945043/1‑150 (MQ=34) gACCCCGAACCCAACGCCAGCTCAGCCCACGCTCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTCCACCGTGACCCTGGGGAATCCTGCCCAGACGCCGGTGACAGt < 1:1184823/150‑1 (MQ=11) cctcccGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGt < 1:630643/147‑1 (MQ=9) aCCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTg > 1:2375139/1‑150 (MQ=32) aCCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTg > 2:1294159/1‑150 (MQ=12) ccccGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGa < 1:1702213/150‑1 (MQ=32) cccGAACCCGACCCCGACTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTCATCACCTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACAGTGAc > 2:712447/1‑150 (MQ=255) ccGAACCCGACGCCGGATCAGACCTTGATCTACGGCTCTTTCAATGCCACCACAGCGTCCCCGAGCTGTCGAG‑‑TTTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAcc < 1:44412/150‑1 (MQ=2) ccGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAcc < 1:94867/150‑1 (MQ=17) ccGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAcc > 2:1363843/1‑150 (MQ=17) aCCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGt < 1:680904/150‑1 (MQ=9) aCCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGt < 2:1079694/150‑1 (MQ=14) cccGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGAGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTc > 1:28081/1‑150 (MQ=11) | ACCGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGA‑C‑GGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTC > NC_002947/196730‑196938 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |