Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,909,160 G→A 5.6% Q45* (CAG→TAG)  PP_1709 ← fumarylacetoacetate hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,909,1600GA 5.6% 322.0 / 6.4 106Q45* (CAG→TAG) PP_1709fumarylacetoacetate hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base G (51/49);  new base A (2/4);  total (53/53)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

Only 100 of 108 total aligned reads displayed.

GCTCGCGGGCGATGTTGGCGCCGCCTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATGGGTGGATCCTCCTTA  >  NC_002947/1909013‑1909308
                                                                                                                                                   |                                                                                                                                                    
gCTCGCGGGCGATGTTGGCGCCGCCTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTgcg                                                                                                                                                    >  2:1924877/1‑150 (MQ=255)
 cTCGCGGGCGATGTTGGCGCCGCCTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTgcgg                                                                                                                                                   <  1:1561182/150‑1 (MQ=255)
         cGATGTTGGCGCCGCCTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGgcgccgcgc                                                                                                                                           >  2:1997518/1‑150 (MQ=255)
            tGTTGGCGCCGCCTTTGGCGATGGCCGCGACCAGCTCAATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGt                                                                                                                                        >  1:1949327/1‑150 (MQ=255)
             gTTGGCGCCGCCTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTa                                                                                                                                       <  1:2398881/150‑1 (MQ=255)
             gTTGGCGCCGCCTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTGCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTa                                                                                                                                       <  2:380097/150‑1 (MQ=255)
              ttGGCGCCGCCTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAg                                                                                                                                      <  2:2404042/150‑1 (MQ=255)
              ttGGCGCCGCCTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTACGGCGCGGCGCCGCAg                                                                                                                                      <  1:2025778/150‑1 (MQ=255)
                ggCGCCGCCTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGa                                                                                                                                    <  1:2165034/150‑1 (MQ=255)
                ggCGCCGCCTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGa                                                                                                                                    <  2:1364364/150‑1 (MQ=255)
                ggCGCCGCCTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTACGGCGCGGCGCCGCAGGa                                                                                                                                    <  2:1789047/150‑1 (MQ=255)
                  cgccgcCTTTGGCGATGGCCGCGCCCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGAtt                                                                                                                                  <  1:742887/150‑1 (MQ=255)
                     cgccTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCg                                                                                                                               <  1:798125/150‑1 (MQ=255)
                     cgccTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCg                                                                                                                               >  2:2008568/1‑150 (MQ=255)
                        cTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGcc                                                                                                                            >  2:1907009/1‑150 (MQ=255)
                         tttGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCa                                                                                                                           >  1:2123169/1‑150 (MQ=255)
                             gCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACggg                                                                                                                       <  2:1511906/150‑1 (MQ=255)
                             gCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACggg                                                                                                                       <  1:1259521/150‑1 (MQ=255)
                               gATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGgcgccgcgcc                                                                                                                                          >  2:1073709/1‑129 (MQ=255)
                               gATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGgcgccgcgcc                                                                                                                                          <  1:1073709/129‑1 (MQ=255)
                               gATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTa                                                                                                                     >  2:1237679/1‑150 (MQ=255)
                                   gCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTg                                                                                                                 >  1:1531253/1‑150 (MQ=255)
                                     cgcgCCAAGCTCTATTTCATTGTAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCg                                                                                                               <  2:139582/150‑1 (MQ=255)
                                          ccAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGCGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGa                                                                                                          <  1:23119/150‑1 (MQ=255)
                                            aGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAgg                                                                                                        >  2:1487339/1‑150 (MQ=255)
                                              cTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCAAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCa                                                                                                      >  1:1267254/1‑150 (MQ=255)
                                               tCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGAtt                                                                                                                                  >  2:2304712/1‑121 (MQ=255)
                                               tCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGAtt                                                                                                                                  <  1:2304712/121‑1 (MQ=255)
                                               tCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAg                                                                                                     >  1:516806/1‑150 (MQ=255)
                                                 gATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGaa                                                                                                   >  2:1029100/1‑150 (MQ=255)
                                                        tGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCgg                                                                                            >  2:2365437/1‑150 (MQ=255)
                                                        tGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCgg                                                                                            >  2:237848/1‑150 (MQ=255)
                                                          gCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGcc                                                                                          >  2:2272356/1‑150 (MQ=255)
                                                          gCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGcc                                                                                          <  2:73751/150‑1 (MQ=255)
                                                           cAGAACTCGTCGGTCTGCGGCGGGAAGGCCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCg                                                                                         >  1:1033069/1‑150 (MQ=255)
                                                               aCTCGTCGGTTTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCg                                                                                     <  1:1006606/150‑1 (MQ=255)
                                                               aCTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCg                                                                                     <  2:1859899/150‑1 (MQ=255)
                                                                       gTCTGCGGCGGGAACGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAgcgc                                                                             <  1:2044026/150‑1 (MQ=255)
                                                                             ggcggGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCg                                                                       >  2:38132/1‑150 (MQ=255)
                                                                                gggAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGtt                                                                                                                  >  1:1072040/1‑104 (MQ=255)
                                                                                gggAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGtt                                                                                                                  <  2:1072040/104‑1 (MQ=255)
                                                                                gggAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTg                                                                    >  1:1476949/1‑150 (MQ=255)
                                                                                 ggAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGc                                                                   >  2:1932031/1‑150 (MQ=255)
                                                                                    aGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGCAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCcgc                                                                >  2:219801/1‑150 (MQ=255)
                                                                                    aGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGCAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCcgc                                                                <  1:219801/150‑1 (MQ=255)
                                                                                      ggCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCgg                                                              >  1:748985/1‑150 (MQ=255)
                                                                                       gCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGa                                                             >  2:2252260/1‑150 (MQ=255)
                                                                                       gCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGATGACGCAGa                                                             <  2:1033069/150‑1 (MQ=255)
                                                                                         aGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATc                                                           <  2:2112589/150‑1 (MQ=255)
                                                                                          gTTCGCCCAGAGCCTCGACCACGTTGCTTGCCGGTTTCAAGAAAAAGACAGGCTCGTGCGGCGCCGCGCCGTAGGATTACGGCGACGGGTAGTTGCGGCCGAGTGAGAACATCCGCCGGACCGGGAAGCGCGCGTCGCTGGCGCGGATCg                                                          <  2:112292/150‑1 (MQ=255)
                                                                                             cGCACAGCGACTCGTCCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCa                                                       <  2:1267254/150‑1 (MQ=255)
                                                                                                ccAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCg                                                                                                                               >  1:731640/1‑75 (MQ=255)
                                                                                                ccAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCg                                                                                                                               <  2:731640/75‑1 (MQ=255)
                                                                                                ccAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCt                                                                     <  1:580776/133‑1 (MQ=255)
                                                                                                ccAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCt                                                                     >  2:580776/1‑133 (MQ=255)
                                                                                                   gttccTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGa                                                 <  1:237848/147‑1 (MQ=255)
                                                                                                   gTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGa                                                 <  2:129388/150‑1 (MQ=255)
                                                                                                       cTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGAt                                             >  1:158560/1‑150 (MQ=255)
                                                                                                        tCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGa                                                                                                          <  1:2112302/88‑1 (MQ=255)
                                                                                                        tCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGa                                                                                                          >  2:2112302/1‑88 (MQ=255)
                                                                                                        tCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATg                                            <  2:2123169/150‑1 (MQ=255)
                                                                                                        tCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATg                                            >  2:1194499/1‑150 (MQ=255)
                                                                                                          gACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAgcgc                                                                             >  2:2159306/1‑115 (MQ=255)
                                                                                                          gACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAgcgc                                                                             <  1:2159306/115‑1 (MQ=255)
                                                                                                          gACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGc                                          >  1:1984526/1‑150 (MQ=255)
                                                                                                          gACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCACCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGGAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGc                                          >  1:1935521/1‑150 (MQ=255)
                                                                                                           aCCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCgg                                                                                                                              <  2:1769629/65‑1 (MQ=255)
                                                                                                           aCCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCgg                                                                                                                              >  1:1769629/1‑65 (MQ=255)
                                                                                                           aCCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGaa                                                                                                   <  1:1743340/92‑1 (MQ=255)
                                                                                                           aCCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGaa                                                                                                   >  2:1743340/1‑92 (MQ=255)
                                                                                                             cACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCcgg                                       >  1:319113/1‑150 (MQ=255)
                                                                                                              aCGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCcggc                                      >  1:317689/1‑150 (MQ=255)
                                                                                                                  tgcttgcCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGt                                  <  2:1687360/150‑1 (MQ=255)
                                                                                                                   gcttgcCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGtt                                 <  2:319113/150‑1 (MQ=255)
                                                                                                                     ttgcCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGa                               >  1:791096/1‑150 (MQ=255)
                                                                                                                      tgcCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGACGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGaa                              <  1:1997518/150‑1 (MQ=255)
                                                                                                                       gcCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGCTGGCCGGCGGGTTGAAc                             >  2:547713/1‑150 (MQ=255)
                                                                                                                          ggTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAg                           <  2:1912635/149‑1 (MQ=255)
                                                                                                                          ggTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAg                           >  1:1912635/1‑149 (MQ=255)
                                                                                                                            tttCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGcc                                         >  1:259909/1‑133 (MQ=255)
                                                                                                                            tttCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTa                        >  2:1438146/1‑150 (MQ=255)
                                                                                                                                   aaaaaCACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATg                 <  1:1438146/150‑1 (MQ=255)
                                                                                                                                   aaaaaCACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATg                 <  2:516806/150‑1 (MQ=255)
                                                                                                                                    aaaaCACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATgg                <  1:2272356/150‑1 (MQ=255)
                                                                                                                                     aaaCACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGACGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATggg               <  2:1476949/150‑1 (MQ=255)
                                                                                                                                       acacAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATc                                                           >  2:754989/1‑104 (MQ=255)
                                                                                                                                       acacAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATc                                                           <  1:754989/104‑1 (MQ=255)
                                                                                                                                       acacAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATGGGTg             >  1:2327352/1‑150 (MQ=255)
                                                                                                                                           aGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATGGGTGGAtc         >  2:2181425/1‑150 (MQ=255)
                                                                                                                                            ggCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATGGGTGGAtcc        <  1:1029100/150‑1 (MQ=255)
                                                                                                                                            gaatCCTACGGCGCGGCGCCGCAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATGGGTGGAtcc        >  1:558991/4‑150 (MQ=255)
                                                                                                                                             gCTCCTGCGGCGCCGTGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCgg                                                              >  2:1980807/1‑95 (MQ=255)
                                                                                                                                             gCTCCTGCGGCGCCGTGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCgg                                                              <  1:1980807/95‑1 (MQ=255)
                                                                                                                                              cTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATGGGTGGAtcctc      <  2:2396509/150‑1 (MQ=255)
                                                                                                                                              atCCTACGGCGCGGCGCCGCAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTaa                       >  1:1645400/2‑133 (MQ=255)
                                                                                                                                               tCCTACGGCGCGGCGCCGCAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATGGGTGGAtcctcc     <  2:558991/150‑1 (MQ=255)
                                                                                                                                                 cTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATGGGTGGAtc         <  1:683129/144‑1 (MQ=255)
                                                                                                                                                 cTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATGGGTGGAtc         >  2:683129/1‑144 (MQ=255)
                                                                                                                                                 cTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATGGGTGGATCCTCCtt   >  1:1847174/1‑150 (MQ=255)
                                                                                                                                                  tGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATGGGTGGATCCTCCTTa  <  2:317689/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCTCGCGGGCGATGTTGGCGCCGCCTTTGGCGATGGCCGCGACCAGCTCGATTTCATGGCAGAACTCGTCGGTCTGCGGCGGGAAGGGCAGTTCGCCCAGTGCCTCGACCACGTTGCTTGCCGGTTTCATGAAAAACACAGGCTCCTGCGGCGCCGCGCCGTAGGATTCCGGCCACGGGTAGTTGCGGCCGAGGCAGAACACCCGGCCGACCGGGAAGCGCGCATCGCTGCCGCGGATCGGCAGGCTGACGATGGCCGGCGGGTTGAACAGGTAAGTCATGGGTGGATCCTCCTTA  >  NC_002947/1909013‑1909308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑