Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,019,126 C→A 7.4% L120M (CTG→ATG) ‡ PP_1797 → HlyD family secretion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,019,1260CA 7.4% 377.8 / 12.8 120L120M (CTG→ATG) ‡PP_1797HlyD family secretion protein
Reads supporting (aligned to +/- strand):  ref base C (50/61);  new base A (5/4);  total (55/65)
Fisher's exact test for biased strand distribution p-value = 7.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01

Only 100 of 121 total aligned reads displayed.

GGTGGCATCGTCAAAGAGCTGCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCGGTCAACCGT  >  NC_002947/2018979‑2019275
                                                                                                                                                   |                                                                                                                                                     
     cATCGTCAAAGAGCTGCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGc                                                                                                                                                >  2:2093821/1‑150 (MQ=255)
        cGTCAAAGAGCTGCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGACCGTTATAAAGAGGCACGCCTGCGTGCCGa                                                                                                                                             <  1:201819/150‑1 (MQ=255)
           cAAAGAGCTGCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCg                                                                                                                                          >  1:247653/1‑150 (MQ=255)
           cAAAGAGCTGCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTAGTAGCTCAGCGCTGGGTATAAGTCTACCCGCAACCAACTGATTGTTGCCCGTTATAATGAGGCACGTCTGCGTGCCGAGCg                                                                                                                                          <  2:842203/150‑1 (MQ=255)
            aaaGAGCTGCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACGGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGt                                                                                                                                         <  1:341660/150‑1 (MQ=255)
              agagCTGCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGa                                                                                                                                       <  2:2397103/150‑1 (MQ=255)
                   tGCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCt                                                                                                                                  <  1:94643/150‑1 (MQ=255)
                    gCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCtt                                                                                                                                 <  1:517183/150‑1 (MQ=255)
                     cATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTg                                                                                                                                >  2:431081/1‑150 (MQ=255)
                     cATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCAACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTg                                                                                                                                >  2:484283/1‑150 (MQ=255)
                       tGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATTCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCTACCCGCAACCAACTGATTGTTGCCCTTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGa                                                                                                                              <  2:443236/150‑1 (MQ=255)
                       tGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGa                                                                                                                              <  2:2135215/150‑1 (MQ=255)
                       tGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGa                                                                                                                              >  1:1798425/1‑150 (MQ=255)
                         cccGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACt                                                                                                                            >  2:2176839/1‑150 (MQ=255)
                          ccGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTc                                                                                                                           >  1:1709866/1‑150 (MQ=255)
                            gcgATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGAGGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGa                                                                                                                         <  2:1206294/150‑1 (MQ=255)
                              gATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCAAAAACCACTAACTGGTTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATc                                                                                                                       >  1:1250334/1‑150 (MQ=255)
                               aTGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATcc                                                                                                                      >  2:839940/1‑150 (MQ=255)
                                  gggACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGt                                                                                                                   <  1:510172/150‑1 (MQ=255)
                                     aCCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGCGTCCCCCCGAACACAACTGATTGTTGCCTGTTGTATAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCgg                                                                                                                <  1:901872/150‑1 (MQ=255)
                                        tGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGa                                                                                                             >  1:2127145/1‑150 (MQ=255)
                                              agaagGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATgg                                                                                                       <  1:1128519/150‑1 (MQ=255)
                                               gaagGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGa                                                                                                      <  1:608077/150‑1 (MQ=255)
                                               gaagGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCAGGCGTGcctct                                                                                                                                            >  2:1119421/1‑109 (MQ=255)
                                               gaagGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCAGGCGTGcctct                                                                                                                                            <  1:1119421/112‑4 (MQ=255)
                                                  gggCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATTGAAgg                                                                                                   <  2:211534/150‑1 (MQ=255)
                                                   ggCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGt                                                                                                  >  1:1298119/1‑150 (MQ=255)
                                                    gCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTa                                                                                                 <  2:1798425/150‑1 (MQ=255)
                                                    gCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTa                                                                                                 <  1:2176839/150‑1 (MQ=255)
                                                     cGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACCCGCGCGAACTCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTAc                                                                                                <  1:1621774/150‑1 (MQ=255)
                                                      gATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTAcc                                                                                               >  2:655671/1‑150 (MQ=255)
                                                       aTCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCg                                                                                              >  1:144041/1‑150 (MQ=255)
                                                        tCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGcc                                                                                                                                               >  2:1121444/1‑100 (MQ=255)
                                                        tCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGcc                                                                                                                                               <  1:1121444/100‑1 (MQ=255)
                                                        tCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGa                                                                                             >  1:2276106/1‑150 (MQ=255)
                                                         ccGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGAt                                                                                            <  2:144041/150‑1 (MQ=255)
                                                            cTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCt                                                                                         <  2:66003/150‑1 (MQ=255)
                                                                ttGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACTAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGTACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGAcc                                                                                     <  1:7045/150‑1 (MQ=255)
                                                                        gatggtgcGCAGCTCAGCGCCGAGTATGAGTCAAAACGCAACGATCAGCTTTTGGACGTTTATAATTAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCaa                                                                             <  2:1601394/143‑1 (MQ=255)
                                                                        gATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGa                                                                                                      >  2:886585/1‑125 (MQ=255)
                                                                        gATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGa                                                                                                      <  1:886585/125‑1 (MQ=255)
                                                                        gATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCaa                                                                             >  2:1195340/1‑150 (MQ=255)
                                                                        gATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCaa                                                                             <  2:1250334/150‑1 (MQ=255)
                                                                             gTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAgc                                                                        >  1:1060103/1‑150 (MQ=255)
                                                                             gTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAgc                                                                        <  2:189300/150‑1 (MQ=255)
                                                                                tcAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCt                                                                     >  2:2212559/2‑150 (MQ=255)
                                                                                gCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGt                                                                                                                                                  >  1:803414/1‑73 (MQ=255)
                                                                                gCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGa                                                                                                                                             <  2:308778/78‑1 (MQ=255)
                                                                                gCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGa                                                                                                                                             >  1:308778/1‑78 (MQ=255)
                                                                                gCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCt                                                                     >  1:1921710/1‑150 (MQ=255)
                                                                                 cAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTGGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTg                                                                    <  2:2258215/150‑1 (MQ=255)
                                                                                    cTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCa                                                                 >  1:2404525/1‑150 (MQ=255)
                                                                                     tCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAg                                                                >  2:2238672/1‑150 (MQ=255)
                                                                                       cgcgcCGAGTATGAGTCCACCCGCAACCAACTCATTGTTGGCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGGCTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGt                                                              <  1:1018029/149‑1 (MQ=255)
                                                                                       aGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGt                                                              <  1:2311673/150‑1 (MQ=255)
                                                                                          gcCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGag                                                           >  2:1828086/1‑150 (MQ=255)
                                                                                           ccGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGaa                                                                                                     <  2:1825660/107‑1 (MQ=255)
                                                                                                    aGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATaa                                                                                                          >  1:1613267/1‑93 (MQ=255)
                                                                                                    aGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATaa                                                                                                          <  2:1613267/93‑1 (MQ=255)
                                                                                                    aGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGt                                                                                                  <  2:241696/101‑1 (MQ=255)
                                                                                                    aGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGt                                                                                                  >  1:241696/1‑101 (MQ=255)
                                                                                                    aGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGTGCAGCAGgtgt                                                 <  2:1298119/150‑1 (MQ=255)
                                                                                                        cACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGa                                                                                                                         <  1:1573428/74‑1 (MQ=255)
                                                                                                         aCCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAg                                            <  2:1060103/150‑1 (MQ=255)
                                                                                                           ccGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGc                                          >  2:1635952/1‑150 (MQ=255)
                                                                                                           ccGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGc                                          >  1:1818774/1‑150 (MQ=255)
                                                                                                            cGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGagcag                                                        >  2:1505594/1‑135 (MQ=255)
                                                                                                            cGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGagcag                                                        <  1:1505594/135‑1 (MQ=255)
                                                                                                               aaccaacTGATTGTTGCCCGTTATAAAGAGGCACGCCt                                                                                                                                                      <  2:734923/38‑1 (MQ=255)
                                                                                                               aaccaacTGATTGTTGCCCGTTATAAAGAGGCACGCCt                                                                                                                                                      >  1:734923/1‑38 (MQ=255)
                                                                                                                  caacTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCAc                                   <  1:1828086/150‑1 (MQ=255)
                                                                                                                   aacTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGa                                                                                                      <  2:840688/82‑1 (MQ=255)
                                                                                                                   aacTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGa                                                                                                      >  1:840688/1‑82 (MQ=255)
                                                                                                                        aTTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCg                             >  2:1455228/1‑150 (MQ=255)
                                                                                                                              gCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTg                       <  1:1635952/150‑1 (MQ=255)
                                                                                                                               cccGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGccc                                        <  2:147857/132‑1 (MQ=255)
                                                                                                                               cccGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGccc                                        >  1:147857/1‑132 (MQ=255)
                                                                                                                               cccGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTgg                      >  2:1323622/1‑150 (MQ=255)
                                                                                                                               ccatttATAAAGATGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTgg                      <  2:2382882/146‑1 (MQ=255)
                                                                                                                                ccGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGagc                                                          >  2:1307454/1‑113 (MQ=255)
                                                                                                                                ccGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGagc                                                          <  1:1307454/113‑1 (MQ=255)
                                                                                                                                ccGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTggg                     <  2:1818774/150‑1 (MQ=255)
                                                                                                                                 cGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGATGTTCAAGGCCCGCCACGATTCGTTGCTGGGc                    >  2:2056222/1‑150 (MQ=255)
                                                                                                                                      taAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAAt               <  2:2116513/150‑1 (MQ=255)
                                                                                                                                        gctcggCACGCAGGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGGGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAAtct             >  2:2351030/5‑150 (MQ=255)
                                                                                                                                         ctcggCACGCAGGCGTGCCTAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGAtt                                                                                           <  1:792047/68‑1 (MQ=38)
                                                                                                                                         ctcggCACGCAGGCGTGCCTAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGAtt                                                                                           >  2:792047/4‑71 (MQ=38)
                                                                                                                                         agagGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGA‑TCTTAACGCGCGGAAAAAACCGTTGGAGGGGAACAGCAGGTGGTTAAAGGCCGGCACGATTCGTTTCTTGGCGGAAttccc           >  1:2317476/1‑146 (MQ=255)
                                                                                                                                          tcggCACGCAGGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAgg                                                               >  1:2321957/3‑98 (MQ=255)
                                                                                                                                          tcggCACGCAGGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAg                                            >  1:535915/3‑117 (MQ=255)
                                                                                                                                          gagGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCg           <  2:2404525/150‑1 (MQ=255)
                                                                                                                                           agGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGa                                                            >  2:1375297/1‑100 (MQ=255)
                                                                                                                                           agGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCgg          <  2:1709866/150‑1 (MQ=255)
                                                                                                                                           agGCACGCCTGCGTGCCGAGCGTGACGGATTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGa                                                            <  1:1375297/100‑1 (MQ=255)
                                                                                                                                            ggCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGCGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGATATCTCGGt         <  2:1602261/150‑1 (MQ=255)
                                                                                                                                             gCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGaaa                >  1:996663/1‑142 (MQ=255)
                                                                                                                                             gCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCGGTc        >  2:492586/1‑150 (MQ=255)
                                                                                                                                                 gCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCg           <  2:671391/143‑1 (MQ=255)
                                                                                                                                                 gCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCg           >  1:671391/1‑143 (MQ=255)
                                                                                                                                                   cTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCGGTCAACCGt  >  1:1246556/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GGTGGCATCGTCAAAGAGCTGCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCGGTCAACCGT  >  NC_002947/2018979‑2019275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑