Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,139,671 A→T 8.0% R165R (CGT→CGA)  PP_5481 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,139,6710AT 8.0% 267.1 / 12.0 89R165R (CGT→CGA) PP_5481hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (39/42);  new base T (5/2);  total (45/44)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01

GCTACGGCATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGTCAG  >  NC_002947/2139522‑2139820
                                                                                                                                                     |                                                                                                                                                     
gCTACGGCATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGcga                                                                                                                                                       >  1:1085763/1‑150 (MQ=255)
gCTACGGCATTATAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTTCACCGGCATTGGcga                                                                                                                                                       >  2:599028/1‑150 (MQ=255)
            tAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGc                                                                                                                                           <  2:1465601/150‑1 (MQ=255)
             aGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCa                                                                                                                                               >  1:2085856/1‑145 (MQ=255)
             aGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCa                                                                                                                                               <  2:2085856/145‑1 (MQ=255)
              ggggTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCg                                                                                                                                         <  2:1647058/150‑1 (MQ=255)
                 gtgtCGGATATCAGCATTTGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGcc                                                                                                                                      >  1:1726024/1‑150 (MQ=255)
                  tgtCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCAGTGGCGTCGTCGCCAATGCCggtgc                                                                                                                                     <  1:1017107/150‑4 (MQ=255)
                   gtCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGccca                                                                                                                                    <  1:1646438/150‑1 (MQ=255)
                      ggATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGcccaccc                                                                                                                                 >  1:2061273/1‑150 (MQ=255)
                           tCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAg                                                                                                                            >  1:2268325/1‑150 (MQ=255)
                              gCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAgcgc                                                                                                                         >  2:2253516/1‑150 (MQ=255)
                                  tGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGTGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGt                                                                                                                     <  2:809751/150‑1 (MQ=255)
                                      aCGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGaa                                                                                                                 >  1:820095/1‑150 (MQ=255)
                                          cGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACAtg                                                                                                             <  1:2095379/150‑1 (MQ=255)
                                          cGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACAtg                                                                                                             <  1:2071614/150‑1 (MQ=255)
                                           gATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCg                                                                                                                                         <  2:917712/121‑1 (MQ=255)
                                           gATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCg                                                                                                                                         >  1:917712/1‑121 (MQ=255)
                                           gATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACAtgg                                                                                                            <  2:1422029/150‑1 (MQ=255)
                                            aTCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACAtggt                                                                                                           <  1:2125243/150‑1 (MQ=255)
                                             tCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACAtggtg                                                                                                          <  1:2253516/150‑1 (MQ=255)
                                                 cTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGgaga                                                                                                      >  1:235187/1‑150 (MQ=255)
                                                 cTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGgaga                                                                                                      >  2:61062/1‑150 (MQ=255)
                                                 cTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGAAACATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGgaga                                                                                                      >  1:2165979/1‑150 (MQ=255)
                                                  ttCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGaa                                                                                                     <  2:1092104/150‑1 (MQ=255)
                                                  ttCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTGCGCTTTGTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGaa                                                                                                     <  1:871397/150‑1 (MQ=255)
                                                      tGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTCCACCGGCATTGGCGACGCCGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAg                                                                                                 <  2:2297041/150‑1 (MQ=255)
                                                        cTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGc                                                                                               <  1:491036/150‑1 (MQ=255)
                                                           gtggCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAg                                                                                            >  1:357547/1‑150 (MQ=255)
                                                              gCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGcgacga                                                                                                                                                    >  1:461993/1‑91 (MQ=255)
                                                              gCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGcgacga                                                                                                                                                    <  2:461993/91‑1 (MQ=255)
                                                               ccACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATg                                                                                        <  2:1329190/150‑1 (MQ=255)
                                                                cACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGc                                                                                       >  1:1005344/1‑150 (MQ=255)
                                                                cACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGc                                                                                       >  1:2156974/1‑150 (MQ=255)
                                                                 aCTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGcc                                                                                      >  2:566102/1‑150 (MQ=255)
                                                                 aCTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGcc                                                                                      <  2:820095/150‑1 (MQ=255)
                                                                 aCTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGcc                                                                                      >  2:816010/1‑150 (MQ=255)
                                                                  cTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGAt                                                                                         >  1:2225452/1‑146 (MQ=255)
                                                                  cTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGAt                                                                                         <  2:2225452/146‑1 (MQ=255)
                                                                   tGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCa                                                                                    <  1:61062/150‑1 (MQ=255)
                                                                   tGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCATGAAGCGGTGCAGCGGCCGTGCCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCa                                                                                    <  1:781317/150‑1 (MQ=255)
                                                                        tGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGga                                                                                                        <  2:1415692/125‑1 (MQ=255)
                                                                        tGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGga                                                                                                        >  1:1415692/1‑125 (MQ=255)
                                                                             tACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGGGTTGCAAAGGCATTGGCGGCCACGCCACTGCTGGCCTACCCACGAGCGCCAGTAGAACATGGTGGCGAAGTAGGCACGGATGCCCAACACCAcgcc                                                                          >  2:1478283/1‑150 (MQ=255)
                                                                             tACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGaa                                                                                                     <  2:1281634/123‑1 (MQ=255)
                                                                             tACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGaa                                                                                                     >  1:1281634/1‑123 (MQ=255)
                                                                               cGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAGCCCTACAGGGATGCCCAGCACCAcgccgc                                                                        >  1:259/1‑150 (MQ=255)
                                                                                    ttagCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCAcacc                                                                   >  2:602707/4‑150 (MQ=255)
                                                                                      tGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCAcaccac                                                                 <  1:8759/150‑1 (MQ=255)
                                                                                          ttCGCTTGCTGGTCAGCGTCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGCGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCAccgag                                                             >  1:631037/1‑150 (MQ=255)
                                                                                          ttCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCAAGCCGCACACCAccgag                                                             >  1:878820/1‑150 (MQ=255)
                                                                                           tCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCAccgagc                                                            <  1:602707/150‑1 (MQ=255)
                                                                                             gCTTGCTGGTCAGCGGCCGCGGCATGCGGGCATGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGCAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCAccgagccg                                                          <  2:759245/150‑1 (MQ=255)
                                                                                                   tGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAgg                                                    >  1:1016899/1‑150 (MQ=255)
                                                                                                     gTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGaa                                                  >  2:697211/1‑150 (MQ=255)
                                                                                                        aGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAggg                                               >  2:2156346/1‑150 (MQ=255)
                                                                                                         gCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACGTGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGt                                              >  2:177282/1‑150 (MQ=255)
                                                                                                         gCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGt                                              <  2:1016899/150‑1 (MQ=255)
                                                                                                                cgGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCcaca                                       >  1:777635/1‑150 (MQ=255)
                                                                                                                 ggCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAg                                      >  2:1313554/1‑150 (MQ=255)
                                                                                                                  gCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGc                                     <  1:599028/150‑1 (MQ=255)
                                                                                                                        gggCCTGAAGCCTTGCACCGGCATTGGCGACGCCGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTAGCCACAGCGTCGAc                               >  2:2364640/1‑150 (MQ=255)
                                                                                                                            cTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCt                           >  1:1759702/1‑150 (MQ=255)
                                                                                                                              gAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTgg                         >  1:2246257/1‑150 (MQ=255)
                                                                                                                               aaGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGt                        >  1:1106620/1‑150 (MQ=255)
                                                                                                                                aaccTTGCACCGGCATTTTCGACGACTCCACTGCCCGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTc                       <  2:878820/148‑1 (MQ=255)
                                                                                                                                 gCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCg                      >  2:1661112/1‑150 (MQ=255)
                                                                                                                                   cTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGCGCCGAGCCGGGCGGGTGGCCACAGCGTCGACAGCTGGTCGGt                    >  2:335940/1‑150 (MQ=255)
                                                                                                                                    ttGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTg                   >  2:1539600/1‑150 (MQ=255)
                                                                                                                                    gtGCACCGGCAGTGGGGACGACGCGACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCAGGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTg                   <  2:654458/149‑1 (MQ=255)
                                                                                                                                     tGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGa                  <  2:235187/150‑1 (MQ=255)
                                                                                                                                     tGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGa                  <  2:2268325/150‑1 (MQ=255)
                                                                                                                                       cACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATc                <  2:991247/150‑1 (MQ=255)
                                                                                                                                       cACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATc                <  2:1005344/150‑1 (MQ=255)
                                                                                                                                          cGGCAGTGGCGTCGTCGCCAATGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCAc             >  1:1587307/1‑150 (MQ=255)
                                                                                                                                            gcagtGGCGTCGTCGCCAATGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAg                                                 <  1:1905358/108‑1 (MQ=255)
                                                                                                                                            gCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGACCACAGCCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTg           <  2:1289667/150‑1 (MQ=255)
                                                                                                                                            gCAGTGGCGTCGTCGCCAATGCCGGCCCACCCACCAGCGCCAGTAGaa                                                                                                                 >  1:2355296/1‑48 (MQ=17)
                                                                                                                                            gCAGTGGCGTCGTCGCCAATGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAg                                                 >  2:1905358/1‑112 (MQ=255)
                                                                                                                                            gCAGTGGCGTCGTCGCCAATGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTg           >  1:1846053/1‑150 (MQ=255)
                                                                                                                                              aTTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGa         >  1:1081836/1‑150 (MQ=255)
                                                                                                                                              aTTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGa         <  2:777635/150‑1 (MQ=255)
                                                                                                                                               ttGGCGACTACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGTTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGTAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGaa        <  2:1726024/150‑1 (MQ=255)
                                                                                                                                               ttGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAg                                                                                                 <  2:2122551/61‑1 (MQ=255)
                                                                                                                                               ttGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAg                                                                                                 >  1:2122551/1‑61 (MQ=255)
                                                                                                                                                tGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAg       <  1:816010/150‑1 (MQ=255)
                                                                                                                                                  gCGTCGTCGCCAATGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGt     >  2:1034172/1‑150 (MQ=255)
                                                                                                                                                  gCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACGTGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGt     <  1:177282/150‑1 (MQ=255)
                                                                                                                                                  gCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCGCACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATc                >  1:727096/1‑139 (MQ=255)
                                                                                                                                                  gCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCGCACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATc                <  2:727096/139‑1 (MQ=255)
                                                                                                                                                  gCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGt     >  1:93162/1‑150 (MQ=255)
                                                                                                                                                  gCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGt     <  2:1759702/150‑1 (MQ=255)
                                                                                                                                                  gCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGt     <  2:2061273/150‑1 (MQ=255)
                                                                                                                                                   cgacgaCGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGTc    <  1:1539600/150‑1 (MQ=255)
                                                                                                                                                    gacgacGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGa                  >  1:477584/1‑135 (MQ=255)
                                                                                                                                                    gacgacGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGa                  <  2:477584/135‑1 (MQ=255)
                                                                                                                                                     acgacgCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGTCAg  <  1:1478283/150‑1 (MQ=255)
                                                                                                                                                     acgacgCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGTCAg  <  2:2156974/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTACGGCATTCTAGGGGTGTCGGATATCAGCATTGGCACGGCGATCACCTTCATGCTGGTGGCCACTGCGGTGCTCTACGTTGTTTGCGTTCGCTTGCTGGTCAGCGGCCGCGGCATGCGGGCCTGAAGCCTTGCACCGGCATTGGCGACGACGCCACTGCCGGCCCACCCACCAGCGCCAGTAGAACATGGTGGAGAAGTAGGCAAGGATGCCCAGCACCACGCCGCACACCACCGAGCCGAGCAGGAAGGGTTGCCACAGCGTCGACAGCTGGTCGGTGATCCACTGGAAGGTCAG  >  NC_002947/2139522‑2139820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑