Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,147,347	A→T	7.7%	A1072A (GCT→GCA)	rne ←	ribonuclease E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,147,347	0	A	T	7.7%	245.4 / 9.8	78	A1072A (GCT→GCA)	rne	ribonuclease E
Reads supporting (aligned to +/- strand):  ref base A (38/34);  new base T (4/2);  total (42/36)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.00e-01

CGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGACAGTGGCAGGTTCGACCACTGCAGGTGCAGGTTCGACCACCGCAG  >  NC_002947/2147198‑2147489                                                                                                                                                        |                                                                                                                                               cGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTgca                                                                                                                                                <  2:440769/150‑1 (MQ=255)  gggTTGACCCGCGAAGAGGCACGTCAGGAAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTGTTTCATTCCGCTTTGGCTATCAGC‑CG‑CGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGATGGTTCCTGGGAAGCTgcag                                                                                                                                               <  2:742775/150‑1 (MQ=255)         cccGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTc                                                                                                                                        <  2:114213/150‑1 (MQ=255)           cgcgAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCt                                                                                                                                      >  1:1951378/1‑150 (MQ=255)                       cGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGCGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTgg                                                                                                                          >  1:1250216/1‑150 (MQ=255)                       cGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGC‑CTCGTCGAG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTgg                                                                                                                          <  1:1955586/150‑1 (MQ=255)                            ggCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGgcagca                                                                                                                     >  2:2150138/1‑150 (MQ=255)                                 aCCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTc                                                                                                                >  1:1974297/1‑150 (MQ=255)                                  ccAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCg                                                                                                               >  2:2271440/1‑150 (MQ=255)                                   cAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCgg                                                                                                              >  2:2324659/1‑150 (MQ=255)                                       aaaaGCCCCGCCAGTGCAAACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTgg                                                                                                                          <  2:1509860/134‑1 (MQ=39)                                       aaaaGCCCCGCCAGTGCAAACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTgg                                                                                                                          >  1:1509860/1‑134 (MQ=39)                                          aGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TCATGAGATTTGTGCTCATCGGCGGCTTCCAGGGCTCGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCg                                                                                                       <  1:1060863/150‑1 (MQ=255)                                             cccGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTgcagca                                                                                                                                             >  2:1225556/1‑109 (MQ=255)                                             cccGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTgcagca                                                                                                                                             <  1:1225556/109‑1 (MQ=255)                                             cccGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCtg                                                                                                                                     <  1:734909/117‑1 (MQ=255)                                             cccGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCtg                                                                                                                                     >  2:734909/1‑117 (MQ=255)                                             cccGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTATGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCtt                                                                                                    >  2:810725/1‑150 (MQ=255)                                             cccGCCAGGTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCtt                                                                                                    >  1:1177414/1‑150 (MQ=255)                                              ccGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTg                                                                                                   >  2:1936512/1‑150 (MQ=255)                                               cGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTg                                                                                                                           <  2:1103907/125‑1 (MQ=255)                                               cGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTg                                                                                                                           >  1:1103907/1‑125 (MQ=255)                                               cGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTgc                                                                                                  <  1:2324659/150‑1 (MQ=255)                                                gCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCGTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTgcg                                                                                                 >  1:1226435/1‑150 (MQ=255)                                                gCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGgcag                                                                                                                       >  2:1987598/1‑128 (MQ=255)                                                gCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGgcag                                                                                                                       <  1:1987598/128‑1 (MQ=255)                                                gCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTgcg                                                                                                 <  2:1974297/150‑1 (MQ=255)                                                         cACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCAc                                                                                        >  2:1258118/1‑150 (MQ=255)                                                         cACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCAc                                                                                        <  2:1177414/150‑1 (MQ=255)                                                         cACTGGCGGGGCTTTTTCATTCAGCTTTGGCTAT‑AGA‑CGTTTATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCAc                                                                                        <  1:126798/150‑1 (MQ=255)                                                          aCTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACt                                                                                       >  1:1715778/1‑150 (MQ=255)                                                          aCTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACt                                                                                       <  1:2150138/150‑1 (MQ=255)                                                          aCTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACt                                                                                       >  2:1084666/1‑150 (MQ=255)                                                            tGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTc                                                                                     >  1:1784841/1‑150 (MQ=255)                                                                 gggCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTg                                                                                <  1:136339/150‑1 (MQ=255)                                                                  ggCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTgg                                                                               <  2:571308/150‑1 (MQ=255)                                                                       tttCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGt                                                                          >  1:2153297/1‑150 (MQ=255)                                                                            ttcaGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGt                                                                     <  1:1321309/150‑1 (MQ=255)                                                                                 cTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACg                                                                <  1:2271440/150‑1 (MQ=255)                                                                                 cTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACg                                                                >  2:2013401/1‑150 (MQ=255)                                                                                  tttGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCtgt                                                                                                                                    >  1:1738726/1‑81 (MQ=255)                                                                                  tttGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCtgt                                                                                                                                    <  2:1738726/81‑1 (MQ=255)                                                                                  tttGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACgg                                                               >  1:1457899/1‑150 (MQ=255)                                                                                     ggCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCa                                                                                         >  2:1700209/1‑121 (MQ=255)                                                                                     ggCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCa                                                                                         <  1:1700209/121‑1 (MQ=255)                                                                                      gCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCAAGGGCTGGTTCCTGGGAATCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGt                                                           >  1:1500894/1‑150 (MQ=255)                                                                                        tATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTgg                                                                               <  1:1435849/128‑1 (MQ=255)                                                                                        tATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTgg                                                                               >  2:1435849/1‑128 (MQ=255)                                                                                         aTCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTgg                                                        >  1:1484137/1‑150 (MQ=255)                                                                                           cAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGcc                                                      <  2:1484137/150‑1 (MQ=255)                                                                                            cgC‑CG‑TGATGACTTTTGTGCTCATCTGCGACTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTTGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCa                                                     <  1:1244154/149‑1 (MQ=255)                                                                                             gC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAg                                                    <  1:336578/150‑1 (MQ=255)                                                                                             gC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAg                                                    <  2:1250216/150‑1 (MQ=255)                                                                                                          gTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGACAGTgg                                         >  1:2126469/1‑150 (MQ=255)                                                                                                                gCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGACAGTGGCAGGtt                                   >  2:1942588/1‑150 (MQ=255)                                                                                                                 cTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGACAGTGGCAGGTTc                                  <  1:2013401/150‑1 (MQ=255)                                                                                                                  tCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGACAGTGGCAGGTTCg                                 >  1:749104/1‑150 (MQ=255)                                                                                                                       ggcggcTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCATTGGCCAGCATGACAGTGGCAGGTTCGACCAc                            <  1:1084666/150‑1 (MQ=255)                                                                                                                          ggcTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGt                                                                     >  1:575479/1‑106 (MQ=255)                                                                                                                          ggcTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGt                                                                     <  2:575479/106‑1 (MQ=255)                                                                                                                           gcTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGACAGTGGCAGGTTCGACCACtgca                        <  1:1936512/150‑1 (MQ=255)                                                                                                                             ttCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAg                                                    >  2:134290/1‑120 (MQ=255)                                                                                                                             ttCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAg                                                    <  1:134290/120‑1 (MQ=255)                                                                                                                              tCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGACAGTGGCAGGTTCGACCACtgcaggt                     <  2:1457899/150‑1 (MQ=255)                                                                                                                                cAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGACAGTGGCAGGTTCGACCACtgcaggtgc                   <  2:2039719/150‑1 (MQ=255)                                                                                                                                     cTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGACAGTGGCAGGTTCGACCACtgca                        >  2:446484/1‑140 (MQ=255)                                                                                                                                     cTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGACAGTGGCAGGTTCGACCACtgca                        <  1:446484/140‑1 (MQ=255)                                                                                                                                         ttCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGACAGTGGCAGGTTCGACCACTGCAGGTGCAGGTTCGac          >  1:832714/1‑150 (MQ=255)                                                                                                                                            cTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCgg                                                                      <  2:407/87‑1 (MQ=255)                                                                                                                                            cTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCgg                                                                      >  1:407/1‑87 (MQ=255)                                                                                                                                            cTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGACAGTGGCAGGTTCGACCACTGCAGGTGCAGGTTCGaccac       >  2:2010796/1‑150 (MQ=255)                                                                                                                                            acaggAAGCTGCTGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGACAGTGGCAGGTTCGACCACTGCAGGTGCAGGTTCGaccac       >  1:1081615/4‑150 (MQ=255)                                                                                                                                            acaggAAGCTGCTGCAGCTTCCTGTGCCGCTTTGGCAAAAGCATCGGCCGCCCGCTTGCGGCTACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGACAGTGGCAGGTTCGACCACTGCAGGTGCAGGTTCGaccac       >  2:84392/4‑150 (MQ=255)                                                                                                                                             tGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGa                                               <  1:1442857/109‑1 (MQ=255)                                                                                                                                             tGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGa                                               >  2:1442857/1‑109 (MQ=255)                                                                                                                                             tGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGACAGTGGCAGGTTCGACCACTGCAGGTGCAGGTTCGaccacc      >  1:2084336/1‑150 (MQ=255)                                                                                                                                             caggAAGCTGCTGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGACAGTGGCAGGTTCGACCACTGCAGGTGCAGGTTCGaccacc      >  2:2250284/3‑150 (MQ=255)                                                                                                                                              gggAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGACAGTGGCAGGTTCGACCACTGCAGGTGCAGGTTCGACCACCg     >  2:2294321/1‑150 (MQ=255)                                                                                                                                              aggAAGCTGCTGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCa                                                  >  1:105355/2‑105 (MQ=255)                                                                                                                                              aggAAGCTGCTGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCa                                                  <  2:105355/104‑1 (MQ=255)                                                                                                                                                gAAGCTGCTGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGACAGTGGCAGGTTCGACCACTGCAGGTGCAGGTTCGACCACCGCa   <  2:1081615/150‑1 (MQ=255)                                                                                                                                                 aaGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCATCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGACAGTGGCAGGTTCGACCACTGCAGGTGCAGGTTCGACCACCGCAg  <  1:871459/150‑1 (MQ=255)                                                                                                                                                      gcagcaGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCg                                                                       >  2:2291765/1‑76 (MQ=255)                                                                                                                                                      gcagcaGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCg                                                                       <  1:2291765/76‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                               CGGGTTTACCCGCGAAGAGGCCCGTCAGGCAAACCAAAAAAAGCCCCGCCAGTTTGCACTGGCGGGGCTTTTTCATTCAGCTTTGGCTATCAGC‑CG‑TGATGAGGTTTGTGCTCATCGGCGGCTTCCAGGGCTGGTTCCTGGGAAGCTGCAGCAGCTTCCTGTGCCGCTTTGGCAGCAGCCTCGGCCTCCCGCTTGCGGCGACGCACTTCACGTGGGTCGTTCGGTGCACGGCCGTTGGCCAGCATGACAGTGGCAGGTTCGACCACTGCAGGTGCAGGTTCGACCACCGCAG  >  NC_002947/2147198‑2147489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑