Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,242,141	G→C	6.0%	noncoding (35/76 nt)	PP_t29 →	tRNA‑Ala

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,242,141	0	G	C	6.0%	261.5 / 5.3	85	noncoding (35/76 nt)	PP_t29	tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base G (46/33);  new base C (3/2);  total (50/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

ACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTC  >  NC_002947/2241996‑2242285                                                                                                                                                  |                                                                                                                                                 acCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTgcatggcatg                                                                                                                                              >  1:222493/1‑150 (MQ=255)  ccGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGcatgccatgc                                                                                                                                             <  2:774319/150‑1 (MQ=255)  ccGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGc                                                                                                                                             >  1:2344036/1‑150 (MQ=255)    gCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCag                                                                                                                                           <  1:335925/150‑1 (MQ=255)       cTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCaggag                                                                                                                                        <  1:1541442/150‑1 (MQ=255)        tttCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCaggagg                                                                                                                                       >  2:1406163/1‑150 (MQ=255)        tttCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCaggagg                                                                                                                                       >  1:441308/1‑150 (MQ=255)            aCGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAg                                                                                                                                   >  1:1993023/1‑150 (MQ=255)             cggcggTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGc                                                                                                                                  >  2:628392/1‑150 (MQ=255)                  gTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTc                                                                                                                             <  1:628392/150‑1 (MQ=255)                  gTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTc                                                                                                                             <  1:2386761/150‑1 (MQ=255)                        ggggTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATccc                                                                                                                       <  1:2200352/150‑1 (MQ=255)                        ggggTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATc                                                                                                                         <  1:665908/148‑1 (MQ=255)                        ggggTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATc                                                                                                                         >  2:665908/1‑148 (MQ=255)                              cGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAg                                                                                                                 <  2:2344036/150‑1 (MQ=255)                               gcgTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGc                                                                                                                >  2:2349844/3‑150 (MQ=255)                               gAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGc                                                                                                                >  1:297123/1‑150 (MQ=255)                               gAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGc                                                                                                                <  2:222493/150‑1 (MQ=255)                                  tCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGTCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTcc                                                                                                             >  2:1252129/1‑150 (MQ=255)                                     ccTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTccacc                                                                                                          >  2:1504386/1‑150 (MQ=255)                                      cTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGAt                                                                                                                          <  2:929160/133‑1 (MQ=255)                                      cTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGAt                                                                                                                          >  1:929160/1‑133 (MQ=255)                                               gCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTGTCGGGGCTGTTGCTCAGCTGGGAGAGCGCCTGCAGGGCATGCGGGAGGTCAGCGGTTCGATGCGGCGTAGCTCCACCATTTCTGcta                                                                                                >  2:2308943/1‑148 (MQ=25)                                                    ttttCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCCGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGtt                                                                                           >  2:1963538/1‑150 (MQ=255)                                                       tCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCAc                                                                                        <  2:297123/150‑1 (MQ=255)                                                       tCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCAc                                                                                        <  2:1463782/150‑1 (MQ=255)                                                         cTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGc                                                                                      >  2:827889/1‑150 (MQ=255)                                                          tGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCa                                                                                     >  1:216927/1‑150 (MQ=255)                                                          tGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCa                                                                                     >  2:2409901/1‑150 (MQ=255)                                                           gCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCaa                                                                                    <  1:708544/150‑1 (MQ=255)                                                             gTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGt                                                                                  <  2:216927/150‑1 (MQ=255)                                                              ttttGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTccac                                                                                                           <  1:2118941/124‑1 (MQ=255)                                                              ttttGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTccac                                                                                                           >  2:2118941/1‑124 (MQ=255)                                                                  gCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGagc                                                                             >  2:1869057/1‑148 (MQ=255)                                                                  gCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATc                                                                             >  2:1612144/1‑150 (MQ=255)                                                                   cAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGACTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCt                                                                            <  1:1758403/150‑1 (MQ=255)                                                                    aGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCtt                                                                           >  2:1963793/1‑150 (MQ=255)                                                                     gggCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTc                                                                          <  2:441308/150‑1 (MQ=255)                                                                     gggCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTc                                                                          <  1:1869057/150‑1 (MQ=255)                                                                        cTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTcgcg                                                                       >  1:708930/1‑150 (MQ=255)                                                                              aaGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAg                                                                    >  1:419366/1‑147 (MQ=255)                                                                              aaGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAg                                                                    <  2:419366/147‑1 (MQ=255)                                                                                    cgtcgATTATTGATGAGCCTTTTGTTTGGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGGCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTcc                                                           >  1:1996715/1‑150 (MQ=255)                                                                                      tcgATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTcccc                                                         >  1:628378/1‑150 (MQ=255)                                                                                        gATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCtt                                                       >  1:1394924/1‑150 (MQ=255)                                                                                          ttattGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCg                                                     >  1:1343582/1‑150 (MQ=255)                                                                                          ttattGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCg                                                     <  1:1406163/150‑1 (MQ=255)                                                                                           tattGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGt                                                    <  2:673477/150‑1 (MQ=255)                                                                                              tgatgaGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTGGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTa                                                 <  1:1963793/150‑1 (MQ=255)                                                                                               gatgaGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAg                                                                    >  1:2076010/1‑130 (MQ=255)                                                                                               gatgaGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAg                                                                    <  2:2076010/130‑1 (MQ=255)                                                                                                 tgaGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTcgc                                                                        <  2:331151/124‑1 (MQ=255)                                                                                                 tgaGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTcgc                                                                        >  1:331151/1‑124 (MQ=255)                                                                                                  gaGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTc                                                                          <  1:1686434/121‑1 (MQ=255)                                                                                                  gaGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTc                                                                          >  2:1686434/1‑121 (MQ=255)                                                                                                     ccTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGGGCGCCTGCATGACCTGGAGGAGGTCCGCGGTTCGCTCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGccc                                          >  2:1775972/1‑149 (MQ=18)                                                                                                      cTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTa                                         >  1:1719133/1‑150 (MQ=255)                                                                                                      cTTTCGTTTCGGGGGGGTAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGCTCGAACCCGCTTAGCACCACCATTTCTGCCAGCGTTCCCGCAAGTGGCTCTTCGCGAACGCTACGTCCCCTTCGTCTAGTGGCCTa                                         <  1:1376000/150‑1 (MQ=18)                                                                                                       ttttGATTCGCGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAg                                        >  2:600545/1‑150 (MQ=255)                                                                                                              tCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCACGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCg                                 <  1:2308943/150‑1 (MQ=255)                                                                                                                   gCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAttt                                                                                                      >  1:949667/1‑76 (MQ=34)                                                                                                                   gCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCAttt                                                                                                      <  2:949667/76‑1 (MQ=34)                                                                                                                      ataGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCa                         >  2:1515956/1‑150 (MQ=255)                                                                                                                         gCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCAcgg                      >  1:311016/1‑150 (MQ=255)                                                                                                                          cTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCAcggc                     <  1:600545/150‑1 (MQ=255)                                                                                                                           tCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCa                                                                                         >  2:615488/1‑81 (MQ=255)                                                                                                                           tCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCa                                                                                         <  1:615488/81‑1 (MQ=255)                                                                                                                           tCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTCCGTCCACATCCGCTATTGGCCTAGGACACCGCCCTTTCAcggcg                    >  2:194875/1‑150 (MQ=18)                                                                                                                                tGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAg                                                                    >  2:146179/1‑97 (MQ=255)                                                                                                                                tGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAg                                                                    <  1:146179/97‑1 (MQ=255)                                                                                                                                tGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAAc               >  1:1155681/1‑150 (MQ=255)                                                                                                                                    agagCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAggg           >  1:2058925/1‑150 (MQ=255)                                                                                                                                     tctctccTGCATGCCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAgggg          <  1:911991/145‑1 (MQ=255)                                                                                                                                       gcgcCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCAACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGtt        >  2:1127712/1‑150 (MQ=255)                                                                                                                                        ctccTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGccc                              >  1:831732/3‑127 (MQ=255)                                                                                                                                        ctccTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGccc                              <  2:831732/125‑1 (MQ=255)                                                                                                                                        ctccTGCATGCCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCAcggcg                    >  2:489028/3‑137 (MQ=255)                                                                                                                                        ctccTGCATGCCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCAcggcg                    <  1:489028/135‑1 (MQ=255)                                                                                                                                        ctccTGCATGCCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTc       >  2:1338826/3‑150 (MQ=255)                                                                                                                                         tccTGCATGCCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCTTCCAGTGGCCTAGGACACCGCCCTTTAACGGCGGGACCAggggagga      >  2:911991/2‑146 (MQ=17)                                                                                                                                          ccTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTTGCTCAAGTGGATCTTACCGAAGGTTACGTCCCCTTCGTTTAGTGGCCTAGGACTCCGCCCTTTCACGGCGGTAACAGGGGTTCGa     <  1:2349844/150‑1 (MQ=17)                                                                                                                                          ccTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTcgc                                                                        <  1:1229222/83‑1 (MQ=255)                                                                                                                                          ccTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTcgc                                                                        >  2:1229222/1‑83 (MQ=255)                                                                                                                                          ccTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGa     >  2:2401103/1‑150 (MQ=255)                                                                                                                                           cTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAg    >  1:1614323/1‑150 (MQ=255)                                                                                                                                           cTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAg    >  2:249514/1‑150 (MQ=255)                                                                                                                                            tGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGt   >  2:1174666/1‑150 (MQ=255)                                                                                                                                             gcatgccatgCAGGCGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCTAGTc  >  1:1300230/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                 ACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCACTTTTCCTGCGTTTTGCAGGGCTTAAAAAGGCTCGTCGATTATTGATGAGCCTTTTGTTTCGGGGCTATAGCTCAGCTGGGAGAGCGCCTGCATGGCATGCAGGAGGTCAGCGGTTCGATCCCGCTTAGCTCCACCATTTCTGCCAGGGTTCACGCAAGTGGATCTTCGCGAAGGTTACGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTC  >  NC_002947/2241996‑2242285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑