Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,283,014 A→T 5.1% W282R (TGG→AGG)  PP_2011 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,283,0140AT 5.1% 293.3 / 4.2 94W282R (TGG→AGG) PP_2011hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (53/36);  new base T (3/2);  total (56/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCACCCAGTCCCGCCGACGAAGCCAGCGCCCCCGCAAATGTTGCGG  >  NC_002947/2282869‑2283161
                                                                                                                                                  |                                                                                                                                                   
ggctggCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCt                                                                                                                                                 <  2:646370/146‑1 (MQ=255)
     gCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCaaa                                                                                                                                            <  1:649325/150‑1 (MQ=255)
     gCGACATCAGTTACCGGGCAGATAGGGACAGCCCTCAGGAAACAAAAAGATCGGTTGTGCCTGCTGCCGGGCACACCCGGCA‑GATCATCGTGCCGGTGCGAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCAACTGCAGCCAGGCTGCaaa                                                                                                                                            >  1:550699/1‑150 (MQ=255)
                 aCCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTg                                                                                                                                >  2:2305081/1‑150 (MQ=255)
                  ccGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGAATCATAGCGGCTTGCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTACAAACTGCTCCGCCTGc                                                                                                                               >  2:593532/1‑150 (MQ=255)
                   cGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCTGGCTGCat                                                                                                                                             <  2:159855/135‑2 (MQ=255)
                   cGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCTGGCTGCat                                                                                                                                             >  1:159855/1‑134 (MQ=255)
                               gCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTg                                                                                                                  >  1:2374467/1‑150 (MQ=255)
                               gCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGAACCAGGTTGAGCAGCGAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTg                                                                                                                  >  1:541622/1‑150 (MQ=255)
                                   gTCACCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTc                                                                                                              <  1:2365814/150‑1 (MQ=255)
                                      atcaCCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCGAc                                                                                                           >  1:1485503/1‑150 (MQ=255)
                                      atcaCCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCAc                                                                                                           >  1:850804/1‑150 (MQ=255)
                                           ccaccaGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCtt                                                                                                       >  2:1475731/1‑149 (MQ=255)
                                           ccaccaGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCtt                                                                                                       <  1:1475731/149‑1 (MQ=255)
                                           ccaccaGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTg                                                                                                      >  1:1444628/1‑150 (MQ=255)
                                           ccaccaGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTg                                                                                                      >  2:105762/1‑150 (MQ=255)
                                           ccaccaGAAACACCCGTTCCGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTg                                                                                                      >  2:1318295/1‑150 (MQ=255)
                                             accaGAAACACCCGTTATGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGcc                                                                                                    >  2:198950/1‑150 (MQ=255)
                                               caGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCgg                                                                                                  >  2:2211471/1‑150 (MQ=255)
                                                 gAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGgcg                                                                                                >  2:1474605/1‑150 (MQ=255)
                                                      acCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCgccagc                                                                                           >  1:1463859/1‑150 (MQ=255)
                                                         cGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGCCTCCACCCTTGCCGGCGccagccag                                                                                        >  1:2008240/1‑150 (MQ=255)
                                                          gTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCgccagcca                                                                                         <  1:2093986/148‑1 (MQ=255)
                                                          gTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCgccagcca                                                                                         >  2:2093986/1‑148 (MQ=255)
                                                          gTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAgg                                                                                       >  1:1639619/1‑150 (MQ=255)
                                                               gCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGc                                                                                  <  2:1534973/150‑1 (MQ=255)
                                                                cctcctGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATg                                                                                   <  2:2354546/148‑1 (MQ=255)
                                                                cctcctGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATg                                                                                   >  1:2354546/1‑148 (MQ=255)
                                                                 ctcctGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGAc                                                                                                                >  2:227839/1‑118 (MQ=255)
                                                                 ctcctGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGAc                                                                                                                <  1:227839/118‑1 (MQ=255)
                                                                 ctcctGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCg                                                                                >  1:2278733/1‑150 (MQ=255)
                                                                  tcctGCCAGGCACCATCGGCATCAGGCTCCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGc                                                                               <  1:1811938/150‑1 (MQ=255)
                                                                  tcctGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGc                                                                               >  2:1063012/1‑150 (MQ=255)
                                                                  tcctGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGc                                                                               >  1:1672641/1‑150 (MQ=255)
                                                                  tcctGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGATGCACACTCCTCCGCCTGACCGCACTCACCTGCCTCCGCCCTTGACGGCGCCAGCCAGGCATGCCGc                                                                               >  1:722967/1‑150 (MQ=255)
                                                                      gCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCa                                                                           <  2:1671496/150‑1 (MQ=255)
                                                                        cAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGc                                                                         >  1:1419995/1‑150 (MQ=255)
                                                                        cAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGc                                                                         >  1:477455/1‑150 (MQ=255)
                                                                         aGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCAc                                                                                                           >  2:1923536/1‑115 (MQ=255)
                                                                         aGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCAc                                                                                                           <  1:1923536/115‑1 (MQ=255)
                                                                         aGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCg                                                                        >  2:2298423/1‑150 (MQ=255)
                                                                         aGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCg                                                                        >  1:1587953/1‑150 (MQ=255)
                                                                            cACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGtt                                                                     <  2:850804/150‑1 (MQ=255)
                                                                             aCCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCGGCAGCGGTTg                                                                    >  1:863343/1‑150 (MQ=255)
                                                                              ccATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGc                                                                   <  2:1047835/150‑1 (MQ=255)
                                                                              ccATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGc                                                                   >  1:2120981/1‑150 (MQ=255)
                                                                                 tCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCTGGCTGCatgt                                                                                                                                           <  2:1691733/75‑4 (MQ=255)
                                                                                 tCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCTGGCTGCatgt                                                                                                                                           >  1:1691733/1‑72 (MQ=255)
                                                                                 tCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGc                                                                                                                                   >  2:1048919/1‑83 (MQ=255)
                                                                                 tCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGc                                                                                                                                   <  1:1048919/83‑1 (MQ=255)
                                                                                 tCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCaa                                                                >  1:2344644/1‑150 (MQ=255)
                                                                                 tCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTACAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCaa                                                                <  1:593532/150‑1 (MQ=255)
                                                                                    gCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCATCCAGGCATGCCGCTGCAGCGGTTGCCAACGc                                                             <  2:1485503/150‑1 (MQ=255)
                                                                                      aTCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTc                                                           <  1:1326620/150‑1 (MQ=255)
                                                                                         aGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGcc                                                        >  1:2234171/1‑150 (MQ=255)
                                                                                         aGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGcc                                                        >  1:2179582/1‑150 (MQ=255)
                                                                                              ccAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCgg                                                   >  1:1388181/1‑150 (MQ=255)
                                                                                                     gTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCa                                             <  2:2008240/150‑1 (MQ=255)
                                                                                                     gTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCa                                             <  2:97146/150‑1 (MQ=255)
                                                                                                     gTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCa                                             <  2:1444628/150‑1 (MQ=255)
                                                                                                     gTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCa                                             >  1:189900/1‑150 (MQ=255)
                                                                                                      tACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGTCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCAc                                            <  2:2299696/150‑1 (MQ=255)
                                                                                                       aCCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCAcc                                           <  2:541622/150‑1 (MQ=255)
                                                                                                       aCCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCAcc                                           <  2:2234171/150‑1 (MQ=255)
                                                                                                         cAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGc                                                                         >  1:1403759/1‑118 (MQ=255)
                                                                                                         cAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGc                                                                         <  2:1403759/118‑1 (MQ=255)
                                                                                                         cAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCACCCa                                         <  1:2298423/150‑1 (MQ=255)
                                                                                                          aGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCtt                                                                                                       >  1:109764/1‑87 (MQ=255)
                                                                                                          aGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCtt                                                                                                       <  2:109764/87‑1 (MQ=255)
                                                                                                           gCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCCCTCACCTGAc                                                                                                                >  2:678397/1‑77 (MQ=255)
                                                                                                           gCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGAc                                                                                                                <  1:678397/77‑1 (MQ=255)
                                                                                                           gCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTc                                                     <  2:310645/136‑1 (MQ=255)
                                                                                                           gCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTc                                                     >  1:310645/1‑136 (MQ=255)
                                                                                                            cAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCACCCAGTc                                      <  1:1318295/150‑1 (MQ=255)
                                                                                                                   gcCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCACCCAGTCCCGCcga                               >  1:763889/1‑150 (MQ=255)
                                                                                                                   gcCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCACCCAGTCCCGCcga                               >  1:2185018/1‑150 (MQ=255)
                                                                                                                     cGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCACCCAGTCCCGCcgacg                             >  2:2049236/1‑150 (MQ=255)
                                                                                                                     cGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCACCCAGTCCCGCcgacg                             >  1:2366199/1‑150 (MQ=255)
                                                                                                                      ggTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCAt                                                                                    >  1:345697/1‑94 (MQ=255)
                                                                                                                      ggTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCAt                                                                                    <  2:345697/94‑1 (MQ=255)
                                                                                                                         gcgtgctgaTGCAGCACATGCAGTGAGGCTGCAAACTGCTCCGCCTGCCAGCACGCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCACCCAGTCCCGCCGACGAAGc                         >  2:399594/4‑150 (MQ=255)
                                                                                                                            tgctgcTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATc                                              <  1:1846934/126‑1 (MQ=255)
                                                                                                                            tgctgcTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATc                                              >  2:1846934/1‑126 (MQ=255)
                                                                                                                             gctgctCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCACCCAGTCCCGCCGACGAAGCCAgc                     >  2:1411439/1‑150 (MQ=255)
                                                                                                                               tgctCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCACCCAGTCCCGCCGACGAAGCCAgcgc                   <  2:722967/150‑1 (MQ=255)
                                                                                                                                 ctCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGCCTCGACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCACCCAGTCCCGCCGACGAAGCCAGCGccc                 >  2:824316/1‑150 (MQ=255)
                                                                                                                                    cAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGc                                                         <  1:209951/107‑1 (MQ=255)
                                                                                                                                    cAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGc                                                         >  2:209951/1‑107 (MQ=255)
                                                                                                                                        acaTGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGGAGCGGTTGCCAACGCTCGCCCTCGGCCATCACCCAGTCCCGCCGACGAAGCCAGCGCCCCCGCaaa          <  2:763889/150‑1 (MQ=255)
                                                                                                                                         tttGCAGCCTGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCACCCAGTCCCGCCGACGAAGCCAGCGCCCCCGCAAAt         >  1:2156828/3‑150 (MQ=255)
                                                                                                                                            gCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCACCCAGTCCCGCCGACGAAGCCAGCGCCCCCGCAAATGtt      >  1:1797011/1‑150 (MQ=255)
                                                                                                                                               gCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCACCCAGTCCCGCCGACGAAGCCAGCGCCCCCGCAAATGTTGCg   <  1:105762/150‑1 (MQ=255)
                                                                                                                                                ccAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCACCCAGTCCCGCCGACGAAGCCAGCGCCCCCGCAAATGTTGCgg  >  1:1926975/1‑150 (MQ=255)
                                                                                                                                                ccAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCACCCAGTCCCGCCGACGAAGCCAGCGCCCCCGCAAATGTTGCgg  <  2:1463859/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCATCAGGCACCAGTC‑GTACCAGCAAGTGCGCCGGTGCCTGCTGCTCCAGCACATGCAGCCAGGCTGCAAACTGCTCCGCCTGCCAGCACTCACCTGACTCCACCCTTGCCGGCGCCAGCCAGGCATGCCGCTGCAGCGGTTGCCAACGCTCGCCCTCGGCCATCACCCAGTCCCGCCGACGAAGCCAGCGCCCCCGCAAATGTTGCGG  >  NC_002947/2282869‑2283161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑