| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 206,739 | T→A | 13.2% | L4082Q (CTG→CAG) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 206,739 | 0 | T | A | 13.2% | 119.6 / 12.8 | 53 | L4082Q (CTG→CAG) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base T (19/27); new base A (2/5); total (21/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
GACGAGCCTGGCACCCCAGGCAATCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGAT > NC_002947/206594‑206824 | gacgaGCCTGGCACCCCAGGCAATCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACTACCTTGTc < 1:2020192/150‑1 (MQ=25)gacgaGCCTGGCACCCCAGGCAATCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTc < 1:1071545/150‑1 (MQ=35) aGCCTGGCACCCCAGGCAATCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGtaa < 1:2020537/150‑3 (MQ=25) aGCCTGGCACCCCAGGCAATCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCAGGTCGTgg < 2:422524/150‑2 (MQ=25) aGCCTGGCACCCCAGGCAATCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCAGGTCGTgg < 1:510740/150‑2 (MQ=25) ccTGGCACCCCAGGCAATCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCAGGTCGTgggc < 2:2251143/150‑4 (MQ=25) tGGCACCCCAGGCAATCCGGGCGGCAGCAACGACGGCGCCCTTCTCAAGTTTATCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCt < 1:1365527/150‑1 (MQ=11) gggtgccAGGCAATCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGATAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGa < 2:2086792/145‑1 (MQ=255) gggtgccAGGCAATCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGa < 1:1286784/145‑1 (MQ=25) gCACCCCAGGCAATCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGa < 1:1774901/150‑1 (MQ=35) gCACCCCAGGCAATCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGa < 1:2237967/150‑1 (MQ=35) aCCCCAGGCAATCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGc < 1:489936/150‑1 (MQ=35) aCCCCAGGCAATCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGAGCCCGAGAACGTCAAACCGACGTTCACAGTCCACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGc > 1:541561/1‑150 (MQ=255) ggCAATCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCaacaac < 1:1895353/150‑1 (MQ=35) ggCAATCCGGGCGGCAGCAACGAAGGCGACCTGGAGAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCaacaac > 1:1999971/1‑150 (MQ=25) gCAATCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACg > 1:1959249/1‑150 (MQ=35) aaTCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGcc < 2:1866539/150‑1 (MQ=35) tCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCa < 1:1178309/150‑1 (MQ=33) gcagctACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCa < 2:550481/150‑1 (MQ=17) cagcaACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCAACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAt > 2:1288227/1‑150 (MQ=17) caACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCaa > 2:298639/1‑150 (MQ=32) aaCGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAg > 1:180303/1‑138 (MQ=33) aaCGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAg < 2:180303/138‑1 (MQ=33) aaCGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAg > 1:1567836/1‑150 (MQ=32) aaCGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAg > 1:832485/1‑150 (MQ=32) aCGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAgg > 1:1726084/1‑150 (MQ=32) aCGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAgg > 2:1663209/1‑150 (MQ=32) aCGAAGGCCACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAgg < 1:882311/150‑1 (MQ=17) gAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCt < 2:2098542/150‑1 (MQ=32) aaGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCAGGTCGTgggcc > 1:1280114/1‑113 (MQ=255) aaGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCAGGTCGTgggcc < 2:1280114/117‑5 (MQ=255) aGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTgg < 2:2016511/150‑1 (MQ=32) ggCGACCTGGTCAAGGTCACCATCACGTCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGc < 2:1758393/150‑1 (MQ=17) ggCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGCGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGc > 2:308158/1‑150 (MQ=17) gACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACTTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCgag < 1:1043966/150‑1 (MQ=17) gACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTggtctc > 2:35271/1‑146 (MQ=17) tGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCa > 1:1690269/1‑150 (MQ=32) gTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAg > 2:1372594/1‑110 (MQ=33) gTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAg < 1:1372594/110‑1 (MQ=33) gTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAAGCTGGCGAGACCAgc > 2:2187432/1‑150 (MQ=17) gTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAAAAGTCGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAgc < 1:1374318/150‑1 (MQ=11) tCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCAGGTCGTggg < 2:957316/103‑3 (MQ=18) tCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCAGGTCGTggg > 1:957316/1‑101 (MQ=18) aGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAgcgcgc < 2:1550842/150‑1 (MQ=32) ggTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGACCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGcc > 1:1957318/1‑150 (MQ=17) gTCACCATCACGGCCGACCAGACTTCGGAGCAAGTCATTGACGTTCCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCg < 2:1999971/150‑1 (MQ=11) cATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTacac > 2:898402/1‑150 (MQ=32) aCGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTACACCCAc < 2:2209360/150‑1 (MQ=32) cGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTACACCCACg > 2:2106730/1‑150 (MQ=32) ggCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACg < 1:339982/101‑1 (MQ=33) ggCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACg > 2:339982/1‑101 (MQ=33) aCTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGt < 1:1024985/150‑1 (MQ=32) tCGGCGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGtgat < 1:308158/150‑1 (MQ=14) | GACGAGCCTGGCACCCCAGGCAATCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGGTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTACACCCACGCTGCGCAGGGTGAT > NC_002947/206594‑206824 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |