Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,309,142	A→G	5.2%	V308A (GTG→GCG)	PP_2032 ←	ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,309,142	0	A	G	5.2%	275.7 / 2.4	90	V308A (GTG→GCG)	PP_2032	ATPase
Reads supporting (aligned to +/- strand):  ref base A (43/42);  new base G (2/3);  total (45/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

TGGCCGGGCTTGGAAAACAGCTGGATTTCGCGCACG‑CGGTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCGGCCAGCCAGGCATGGGCACGCGCGCAGCGGTCCAGC  >  NC_002947/2308994‑2309291                                                                                                                                                      |                                                                                                                                                       tGGCCGGGCTTGGAAAACAGCTGGATTTCGCGCACG‑CGGTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCAc                                                                                                                                                       >  2:1309838/1‑150 (MQ=255)   gCCGGGCTTGGAAAACAGCTGGATTTCGCGCACG‑CGGTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCAcgc                                                                                                                                                     >  1:725820/1‑150 (MQ=255)    ccGGGCTTGGAAAACAGCTGGATTTCGCGCACG‑CGGTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCAcgcg                                                                                                                                                    >  2:2119465/1‑150 (MQ=255)      gggCTTGGAAAACAGCTGGATTTCGC‑CAGGACGGTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGt                                                                                                                                                  >  1:63377/1‑150 (MQ=255)         cTTGGAAAACAGCTGGATTTCGCGCACG‑CGGTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGTCGTCGAGGATGCGCTGGGCCACGCGGT‑CCt                                                                                                                                              <  2:143074/150‑1 (MQ=255)          ttGGAAAACAGCTGGATTTCGCGCACG‑CGGTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCGCGTGGT‑ccag                                                                                                                                             <  2:1618297/150‑3 (MQ=255)          ttGGAAAACAGCTGGATTTCGCGCACG‑CGGTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTg                                                                                                                                             >  1:92306/1‑150 (MQ=255)          ttGGAAAACAGCTGGATTTCGCGCACG‑CGGTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTg                                                                                                                                             <  2:2359959/150‑1 (MQ=255)          ttGGAAAACAGCTGGATTTAGCGCACG‑CGGTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTg                                                                                                                                             >  2:825239/1‑150 (MQ=255)          ttGGAAAACAGCTGGAGTTCGCGCACG‑CGGTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTAGCCCCGCTGCGCAGTGCGCATGGGTTCAGGTAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTg                                                                                                                                             <  1:1149541/150‑1 (MQ=255)            ggAAAACAGCTGGATTTCGCGCACG‑CGGTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGt                                                                                                                                           >  1:2318578/1‑150 (MQ=255)                 aCAGCTGGATTTCGCGCACG‑CGGTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATc                                                                                                                                      <  2:725820/150‑1 (MQ=255)                    gCTGGATTTCGCGCACG‑CGGTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCt                                                                                                                                   <  2:1964289/150‑1 (MQ=255)                     cTGGATTTCGCGCACG‑CGGTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTg                                                                                                                                  >  1:1823729/1‑150 (MQ=255)                     cTGGATTTCGCGCACG‑CGGTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTAAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCAc                                                                                                                                                       <  2:681341/130‑1 (MQ=255)                     cTGGATTTCGCGCACG‑CGGTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTAAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCAc                                                                                                                                                       >  1:681341/1‑130 (MQ=255)                          tttCGCGCACG‑CGGTGGCGCATGTCGATCAGCTCGGCAAGGCCACTGCGCTGGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTgccgcc                                                                                                                             >  1:1640851/1‑150 (MQ=255)                               cgcACG‑CGGTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCgcctcggc                                                                                                                        <  1:362284/150‑1 (MQ=255)                                    g‑cgGTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGa                                                                                                                   >  2:2231111/1‑150 (MQ=255)                                    g‑cgGTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGa                                                                                                                   >  1:350354/1‑150 (MQ=255)                                       ggTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAAc                                                                                                                 <  2:2285959/150‑1 (MQ=255)                                             gcATGTCGATCAGCTAGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCTGCGAACGAGAgg                                                                                                           <  2:760831/150‑1 (MQ=255)                                                tGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGgatg                                                                                                        >  2:826889/1‑150 (MQ=255)                                                    gatcagatcaGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGTATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGc                                                                                                    >  2:2278581/1‑150 (MQ=255)                                                    gATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGCTCCCTGCCCCCTCTGCCCCCAAGGAGAGCATTATGc                                                                                                    >  2:1122363/1‑150 (MQ=255)                                                      tCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCgg                                                                                                  >  1:337659/1‑150 (MQ=255)                                                        aGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTg                                                                                                <  2:1589098/150‑1 (MQ=255)                                                         gCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGTTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTgg                                                                                               >  2:880884/1‑150 (MQ=255)                                                         gCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTgg                                                                                               >  2:2096312/1‑150 (MQ=255)                                                         gCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTgg                                                                                               >  1:512581/1‑150 (MQ=255)                                                           tCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGgcg                                                                                             >  1:751196/1‑150 (MQ=255)                                                             ggCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCa                                                                                           <  2:1204305/150‑1 (MQ=255)                                                             ggCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCa                                                                                           <  2:787567/150‑1 (MQ=255)                                                               cAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGt                                                                                         <  1:826889/150‑1 (MQ=255)                                                               cAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGt                                                                                         <  2:2033129/150‑1 (MQ=255)                                                               cAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCAGTACCTGG‑CGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGt                                                                                         <  1:1376422/150‑1 (MQ=255)                                                                  ggCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACg                                                                                      <  2:350354/150‑1 (MQ=255)                                                                    ccAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGACATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTc                                                                                    >  1:686962/1‑150 (MQ=255)                                                                     cAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCg                                                                                   <  1:825239/150‑1 (MQ=255)                                                                      aaTGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCTAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGa                                                                                  >  1:956423/1‑150 (MQ=255)                                                                        tGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAAc                                                                                <  2:512581/150‑1 (MQ=255)                                                                             aTGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCAc                                                                           <  2:492348/150‑1 (MQ=255)                                                                                 cGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGGTGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCgcctcggc                                                                                                                        >  2:1726511/1‑101 (MQ=255)                                                                                 cGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGGTGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCgcctcggc                                                                                                                        <  1:1726511/101‑1 (MQ=255)                                                                                 cGTGTTCGGCCAACTGCGAAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGcc                                                                       >  2:1192372/1‑150 (MQ=255)                                                                                  gtgtTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCt                                                                      <  2:1823729/150‑1 (MQ=255)                                                                                   tgtTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTg                                                                     <  2:1332682/150‑1 (MQ=255)                                                                                      tCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGAGACGACGTCGCGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGAt                                                                  <  2:200142/150‑1 (MQ=255)                                                                                      tCGGCCAGCTGCGCAGTGGGCATGGGTGCAGGCAAGGGCGCAGAGCTACCTCAAGCTGTTGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGAt                                                                  <  2:63377/150‑1 (MQ=255)                                                                                          ccAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGt                                                                                         <  2:1451940/123‑1 (MQ=255)                                                                                          ccAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGt                                                                                         >  1:1451940/1‑123 (MQ=255)                                                                                           cAGCTGCGCAGTGGACATGTGTTCAGGCACCGGCGAAGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGACGCCTCGGGCTCGAACGAGAGGATGATGCGGTGGCGCCGTACGTCGAACAGCACGGCCTGGATGTCtt                                                             >  2:270155/1‑150 (MQ=255)                                                                                             gCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCg                                                           <  2:1812355/150‑1 (MQ=255)                                                                                                gcgcAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCgggg                                                        >  1:221631/1‑150 (MQ=255)                                                                                                     gTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGAc                                                   <  2:337659/150‑1 (MQ=255)                                                                                                        ggCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGaa                                                >  2:2112834/1‑150 (MQ=255)                                                                                                         gCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAg                                               <  2:1294162/150‑1 (MQ=255)                                                                                                          cATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGt                                              >  2:659017/1‑150 (MQ=255)                                                                                                           aTGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTc                                             <  2:589838/150‑1 (MQ=255)                                                                                                                ttCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGcc                                        >  1:942725/1‑150 (MQ=255)                                                                                                                     gCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGTGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCGgcc                                   >  2:127692/1‑150 (MQ=255)                                                                                                                     gCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTg                                                                                                >  1:825022/1‑89 (MQ=255)                                                                                                                     gCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTg                                                                                                <  2:825022/89‑1 (MQ=255)                                                                                                                     gCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCGgcc                                   >  2:754301/1‑150 (MQ=255)                                                                                                                          ggCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCGgccagcca                              <  1:1310845/150‑1 (MQ=255)                                                                                                                          ggCGACGACGTCGAGGATGAGCTGGACCACGCGGT‑CCTGGTCGAt                                                                                                                                       >  1:91469/1‑45 (MQ=255)                                                                                                                          ggCGACGACGTCGAGGATGAGCTGGACCACGCGGT‑CCTGGTCGAt                                                                                                                                       <  2:91469/45‑1 (MQ=255)                                                                                                                           gCGACGACGTGGAGGAGGCGGTGGACCGCGCGGT‑CCTGGGCGGGGGGGGGCGCCTCGGGCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCGGccagccag                             <  1:880884/150‑1 (MQ=255)                                                                                                                           gCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAGCGAGAGGATGATGCGGTTGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCGGccagccag                             <  2:1280885/150‑1 (MQ=255)                                                                                                                           gCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCGGccagccag                             <  1:754301/150‑1 (MQ=255)                                                                                                                             gacgacGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCgg                                                          >  1:1503931/1‑119 (MQ=255)                                                                                                                             gacgacGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCgg                                                          <  2:1503931/119‑1 (MQ=255)                                                                                                                             gacgacGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCGGCCAGCCAGGc                           <  2:92306/150‑1 (MQ=255)                                                                                                                             gacgacGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCGGCCAGCCAGGc                           <  1:2118107/150‑1 (MQ=255)                                                                                                                                 acgTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCGGCCAGCCAGGCATgg                       <  2:213635/150‑1 (MQ=255)                                                                                                                                  cgTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCGGCCAGCCAGGCATggg                      <  1:123644/150‑1 (MQ=255)                                                                                                                                   gTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTc                                             >  1:1179487/1‑126 (MQ=255)                                                                                                                                   gTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTc                                             <  2:1179487/126‑1 (MQ=255)                                                                                                                                       aGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCGGCCAGCCAGGCATGGGCAcgc                 <  2:686962/150‑1 (MQ=255)                                                                                                                                              gcTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCGGCCAGCCAGGCATGGGCACGCGCGCAGc          >  1:1088495/1‑150 (MQ=255)                                                                                                                                              gcTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCGGCCAGCCAGGCATGGGCACGCGCGCAGc          >  2:1054093/1‑150 (MQ=255)                                                                                                                                              gcTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAAAGAGAGGGTGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGCTGTCTTCGGGGCTGACGAAGTCGCGCCCGGCCAGCCAGGCATGGGCACGCGCGCAGc          >  2:1375651/1‑150 (MQ=255)                                                                                                                                              gcTGGACCACGCGGC‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCGgccagc                                >  1:652398/1‑128 (MQ=255)                                                                                                                                              gcTGGACCACGCGGC‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCGgccagc                                <  2:652398/128‑1 (MQ=255)                                                                                                                                              ccaggACCGCGTGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGCCGAAGTCGCGCCCGGCCAGCCAGGCATAGGCATGCGCGCAGc          >  2:471576/4‑150 (MQ=255)                                                                                                                                              ccaggACCGCGTGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCg                                      <  1:133137/119‑1 (MQ=255)                                                                                                                                              ccaggACCGCGTGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCg                                      >  2:133137/4‑122 (MQ=255)                                                                                                                                                tGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCGGCCAGCCAGGCATGGGCACGCGCGCAGCgg        >  1:75136/1‑150 (MQ=255)                                                                                                                                                 ggACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAATACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCGGCCAGCCAGGCATGGGCACGCGCGCAGCGGt       >  1:1714363/1‑150 (MQ=255)                                                                                                                                                     cACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCGGGATGTCTTCGGGGCTGACGAAGTCGCGCCCGGCCAGGCAGGCGTGGTCACGCGCGCAGCGGTCCAg   >  1:1133705/1‑150 (MQ=255)                                                                                                                                                      aCGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCGGCCAGCCAGGCATGGGCACGCGCGCAGCGGTCCAGc  <  2:1074341/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                       TGGCCGGGCTTGGAAAACAGCTGGATTTCGCGCACG‑CGGTGGCGCATGTCGATCAGCTCGGCAAGGCCAATGCGGATGCCGTGTTCGGCCAGCTGCGCAGTGGGCATGGGTTCAGGCAACGGCGACGACGTCGAGGATGCGCTGGACCACGCGGT‑CCTGGTCGATCCCTGCCGCCTCGGCCTCGAACGAGAGGATGATGCGGTGGCGCAGTACGTCGAACAGCACGGCCTGGATGTCTTCGGGGCTGACGAAGTCGCGCCCGGCCAGCCAGGCATGGGCACGCGCGCAGCGGTCCAGC  >  NC_002947/2308994‑2309291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑