breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsPputida_TALE__HGL_Pputida_123_S111_L001_R1_001.good.fq3,969,757578,876,607100.0%145.8 bases150 bases96.4%
errorsPputida_TALE__HGL_Pputida_123_S111_L001_R2_001.good.fq3,969,757578,876,607100.0%145.8 bases150 bases95.8%
total7,939,5141,157,753,214100.0%145.8 bases150 bases96.1%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0029476,181,929179.63.4100.0%Pseudomonas putida KT2440 chromosome, complete genome.
total6,181,929100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 100000100000
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 3
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 50001632
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.082

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0029470.63704

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input01:49:33 30 Mar 202001:51:40 30 Mar 20202 minutes 7 seconds
Read alignment to reference genome01:51:40 30 Mar 202002:09:55 30 Mar 202018 minutes 15 seconds
Preprocessing alignments for candidate junction identification02:09:55 30 Mar 202002:11:48 30 Mar 20201 minute 53 seconds
Preliminary analysis of coverage distribution02:11:48 30 Mar 202002:15:33 30 Mar 20203 minutes 45 seconds
Identifying junction candidates02:15:33 30 Mar 202002:41:59 30 Mar 202026 minutes 26 seconds
Re-alignment to junction candidates02:41:59 30 Mar 202002:47:36 30 Mar 20205 minutes 37 seconds
Resolving best read alignments02:47:36 30 Mar 202002:51:05 30 Mar 20203 minutes 29 seconds
Creating BAM files02:51:05 30 Mar 202002:54:13 30 Mar 20203 minutes 8 seconds
Tabulating error counts02:54:13 30 Mar 202002:55:51 30 Mar 20201 minute 38 seconds
Re-calibrating base error rates02:55:51 30 Mar 202002:55:53 30 Mar 20202 seconds
Examining read alignment evidence02:55:53 30 Mar 202003:13:11 30 Mar 202017 minutes 18 seconds
Polymorphism statistics03:13:11 30 Mar 202003:13:17 30 Mar 20206 seconds
Output03:13:17 30 Mar 202003:14:33 30 Mar 20201 minute 16 seconds
Total 1 hour 25 minutes 0 seconds