Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 5,610,405 A→C V54G (GTC→GGC)  PP_4929 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,610,4050AC89.3% 78.6 / ‑0.1 28V54G (GTC→GGC) PP_4929LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base C (12/13);  total (14/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

GCCTCCTGCCCGCGCATCAGGGCCAGGGCGCGGCCATCCAGGCGTTCGCACTGGCGTAACAATTCGCGGGCGTCCTCCAGCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGACCCCCAGGTCGGTTTCGAGCAGCGCGATGGCACTGCTGATCGCCGACTGGGCTTTGTGCTGCTCACGTGCCACCGCGGAGAACGAGCGCAGTTCTGCAACGCGCAGAAAGGTACGCAATTGTTCAAGGTTCCACTGCTCGG  >  NC_002947/5610264‑5610546
                                                                                                                                             |                                                                                                                                             
gCCTCCTGCCCGCGCATCAGGGCCAGGGCGCGGCCATCCAGGCGTTCGCACTGGCGTAACAATTCGCGGGCGTCCTCCAGCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAgg                                                                                                                                       >  2:749287/1‑150 (MQ=255)
           gcgcATCAGGGCCAGGGCGCGGCCATCCAGGCGTTCGCACTGGCGTAACAATTCGCGGGCGTCCTCCAGCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAGGTCGGTTTCGag                                                                                                                            >  1:147286/1‑150 (MQ=255)
                tCAGGGCCAGGGCGCGGCCATCCAGGCGTTCGCACTGGCGTAACAATTCGCGGGCGTCCTCCAGCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAg                                                                                                                                        >  1:766734/1‑133 (MQ=255)
                tCAGGGCCAGGGCGCGGCCATCCAGGCGTTCGCACTGGCGTAACAATTCGCGGGCGTCCTCCAGCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAg                                                                                                                                        <  2:766734/133‑1 (MQ=255)
                tCAGGGCCAGGGCGCGGCCATCCAGGCGTTCGCACTGGCGTAACAATTCGCGGGCGTCCTCCAGCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAGGTCGGTTTCGAGCAgcg                                                                                                                       >  1:97457/1‑150 (MQ=255)
                         gggcGCGGCCATCCAGGCGTTCGCACTGGCGTAACAATTCGCGGGCGTCCTCCAGCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAGGTCGGTTTCGAGCAGCGCGATGGCAc                                                                                                              >  2:793475/1‑150 (MQ=255)
                         gggcGCGGCCATCCAGGCGTTCGCACTGGCGTAACAATTCGCGGGCGTCCTCCAGCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAGGTCGGTTTCGAGCAGCGCGATGGCAc                                                                                                              >  2:601234/1‑150 (MQ=255)
                         gggcGCGGCCATCCAGGCGTTCGCACTGGCGTAACAATTCGCGGGCGTCCTCCAGCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAGGTCGGTTTCGAGCAGCGCGATGGCAc                                                                                                              <  2:548639/150‑1 (MQ=255)
                              cgGCCATCCAGGCGTTCGCACTGGCGTAACAATTCGCGGGCGTCCTCCAGCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAGGTCGGTTTCGAGCAGTGCCATCGCgctgctc                                                                                                         <  1:534012/150‑2 (MQ=255)
                                   aTCCAGGCGTTCGCACTGGCGTAACAATTCGCGGGCGTCCTCCAGCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAGGTCGGTTTCGAGCAGCGCGATGGCACTGCTGATCGc                                                                                                    <  2:147286/150‑1 (MQ=255)
                                   aTCCAGGCGTTCGCACTGGCGTAACAATTCGCGGGCGTCCTCCAGCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGACCCCCAGGTCGGTTTCGAGCAGCGCGATGGCACTGCTGATCGc                                                                                                    >  2:214463/1‑150 (MQ=255)
                                                  cTGGCGTAACAATTCGCGGGCGTCCTCCAGCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAGGTCGGTTTCGAGCAGCGCGATGGCACTGCTGATCGCCGACTGGGCTTtgtg                                                                                     <  2:633896/150‑1 (MQ=255)
                                                        tAACAATTCGCGGGCGTCCTCCAGCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAGGTCGGTTTCGAGCAGCGCGATGGCACTGCTGATCGCCGACTGGGCTTTGTGCTGCTc                                                                               >  2:103398/1‑150 (MQ=255)
                                                            cctaagcgAGCGCCCTCCACGAAGGCGCTGCTGTTTTAAGTCAACTTGGGTTGCAGGCCGCAGCTGCGCTCGAACAGGGAGCCCCCCAGGTCGGTTTCGAGCAGCGCGATGGCACTGCTGATCGCCAAATGGGCTTTGTGCTGCTCACGt                                                                           >  2:94623/6‑150 (MQ=255)
                                                            aaTTCGCGGGCGTCCTCCAGCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAGGTCGGTTTCGAGCAGCGCGATGGCACTGCTGATCGCCGACTGGGCTTTGTGCTGCTCACGt                                                                           <  1:749287/150‑1 (MQ=255)
                                                              ttCGCGGGCGTCCTCCAGCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAGGTCGGTTTCGAGCAGCGCTATGGCACTGCTGATCGCCGACTGAGCTTTGTGCTGCTCACGTGc                                                                         >  2:97211/1‑150 (MQ=255)
                                                                             cAGCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAGGTCGGTTTCGa                                                                                                                             <  1:202745/83‑1 (MQ=255)
                                                                             cAGCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAGGTCGGTTTCGa                                                                                                                             >  2:202745/1‑83 (MQ=255)
                                                                             cAGCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAGGTCGGTTTCGAGCAGCGCGATGGCACTGCTGATCg                                                                                                     >  2:248345/1‑107 (MQ=255)
                                                                             cAGCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAGGTCGGTTTCGAGCAGCGCGATGGCACTGCTGATCg                                                                                                     <  1:248345/107‑1 (MQ=255)
                                                                               gCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAGGTCGGTTTCGAGCAGCGCGATGGCACTGCTGATCGCCGACTGGGCTTTGTGCTGCTCACGTGCCACCGCGGAGAACGAgc                                                        >  1:90579/1‑150 (MQ=255)
                                                                                    gcgcTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAGGTCGGTTTCGAGCAGCGCGATGGCACTGCTGATCGCCGACTGGGCTTTGTGCTGCTCACGTGCCACCGCGGAGAACGAGCGCAGt                                                   <  2:240690/150‑1 (MQ=255)
                                                                                                      aaTTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAGGTCGGTTTCGAGCAGCGCGATGGCACTGCTGATCGCCGACTGGGCTTTGTGCTGCTCACGTGCCACCGCGGAGAACGAGCGCAGTTCTGCAACGCGCAGAAAg                                 <  1:793475/150‑1 (MQ=255)
                                                                                                        tttCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGCCCCCCAGGTCGGTTTCGAGCAGCGCGATGGCACTGCTGATCGCCGACTGGGCTTTGTGCTGCTCACGTGCCACCGCGGAGAACGAGCGCAGTTCTGCAACGCGCAGAAAGGt                               <  2:97457/150‑1 (MQ=255)
                                                                                                                 ccggccgCTGCTGCGCTCGAACAGAGTGACCCCCAGGTCGGTTTCGAGCAGCGCGATGGCACTGCTGATCGCCGACTGGGCTTTGTGCTGCTCACGTGCCACCGCGGAGAACGAGCGCAGTTCTGc                                              >  2:61136/1‑126 (MQ=255)
                                                                                                                 ccggccgCTGCTGCGCTCGAACAGAGTGACCCCCAGGTCGGTTTCGAGCAGCGCGATGGCACTGCTGATCGCCGACTGGGCTTTGTGCTGCTCACGTGCCACCGCGGAGAACGAGCGCAGTTCTGc                                              <  1:61136/126‑1 (MQ=255)
                                                                                                                                  cGAACAGAGTGCCCCCCAGGTCGGTTTCGAGCAGCGCGATGGCACTGCTGATCGCCCACTGGGCTTTGTCCTGCTCACGTGCCACCGCGGAGAACGAGCGCAGTTCTGCAACGCGAAGAAAGGTACGCAATTGTTCAAGGTTCCACTGCt     <  1:76060/150‑1 (MQ=255)
                                                                                                                                     aCAGAGTGCCCCCCAGGTCGGTTTCGAGCAGCGCGATGGCACTGCTGATCGCCGACTGGGCTTTGTGCTGCTCACGTGCCACCGCGGAGAACGAGCCCAGCTCTCCAACGCGCAGAAAGGTACGCAATTGTTCAAGGTTCCACTGCTCgg  <  1:421828/150‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                             
GCCTCCTGCCCGCGCATCAGGGCCAGGGCGCGGCCATCCAGGCGTTCGCACTGGCGTAACAATTCGCGGGCGTCCTCCAGCAAGGCGCTGCCGTTTTCAGTCAATTTCGGTTGCCGGCCGCTGCTGCGCTCGAACAGAGTGACCCCCAGGTCGGTTTCGAGCAGCGCGATGGCACTGCTGATCGCCGACTGGGCTTTGTGCTGCTCACGTGCCACCGCGGAGAACGAGCGCAGTTCTGCAACGCGCAGAAAGGTACGCAATTGTTCAAGGTTCCACTGCTCGG  >  NC_002947/5610264‑5610546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑