Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,867,794	Δ1 bp	5.3%	intergenic (‑33/+19)	thyA ← / ← lgt	thymidylate synthase/prolipoprotein diacylglyceryl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,867,794	0	G	.	5.3%	231.7 / 7.2	76	intergenic (‑33/+19)	thyA/lgt	thymidylate synthase/prolipoprotein diacylglyceryl transferase
Reads supporting (aligned to +/- strand):  ref base G (37/34);  new base . (2/2);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.76e-01

GAAACGCAGCATCGCGCCCGGCAGGCTGATGGTGCGGATGCCGGTGCGGTTTCCCTGCAACGTGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACTGGCCTTGAGTGAGCCAGCCGAACGCGATGTAACCGAGTTGT  >  NC_002947/5867654‑5867939                                                                                                                                              |                                                                                                                                                  gAAACGCAGCATCGCGCCCGGCAGGCTGATGGTGCGGATGCCGGTGCGGTTTCCCTGCAACGTGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCg                                                                                                                                          >  1:567825/1‑150 (MQ=255) gAAACGCAGCATCGCGCCCGGCAGGCTGATGGTGCGGATGCCGGTGCGGTTTCCCTGCAACGTGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCg                                                                                                                                          >  2:1178698/1‑150 (MQ=255)  aaaCGCAGCATCGCGCCCGGCAGGCTGATGGTGCGGATGCCGGTGCGGTTTCCCTGCAACGTGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGt                                                                                                                                         >  2:802742/1‑150 (MQ=255)    aCGCAGCATCGCGCCCGGCAGGCTGATGGTGCGGATGCCGGTGCGGTTTCCCTGCAACGTGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTgg                                                                                                                                       <  2:1885142/150‑1 (MQ=255)    aCGCAGCATCACGCCCGGCAGACTGATGGTGCCGATTCCCGTGCATTTTCCCTGCAACGTTCCGTTATCAATGACCTCCCGGACCAGGTCCACATACTGTTTCATGGCTTACCTGTAACAAAAACGGCAGGGG‑GATCGCCCCTGCCGTgg                                                                                                                                       <  2:152614/150‑1 (MQ=255)      gcagcaTCGCGCCCGGCAGGGTGATGGCGCGGGTGCCGGGGCGGTTGCCCTGCAACGTGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCCGTAGCAGAGACGGCAGGGG‑GATCGCCCCTGCCGTgggg                                                                                                                                     >  2:1123747/1‑149 (MQ=255)       cagcaTCGCGCCCGGCAGGCTGATGGTGCGGATGCCGGTGCGGTTTCCCTGCAACGTGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGAt                                                                                                                                    >  1:1780101/1‑150 (MQ=255)         gcaTCGCGCCCGGCAGGCTGATGGTGCGGATGCCGGTGCGGTTTCCCTGCAACGTGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTa                                                                                                                                  <  2:1922238/150‑1 (MQ=255)                              tGGTGCGGATGCCGGTGCGGTTTCCCTGCAACGTGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTa                                                                                                              <  1:12899/149‑1 (MQ=255)                              tGGTGCGGATGCCGGTGCGGTTTCCCTGCAACGTGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTa                                                                                                              >  2:12899/1‑149 (MQ=255)                                         ccGGTGCGGTTTCCCTGCAACGTGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATta                                                                                                  <  1:494814/150‑1 (MQ=255)                                          cGGTGCGGTTTCCCTGCAACGTGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATtat                                                                                                 >  2:778992/1‑150 (MQ=255)                                           ggTGCGGTTTCCCTGCAACGTGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATtata                                                                                                <  2:387206/150‑1 (MQ=255)                                              gCGGTTTCCCTGCAACGTGCCGTTATCAATTACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTCCCTGGAGCAAAAACGGCAGGGG‑GATCGCCCCTGCTGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGc                                                                                             <  1:1123747/150‑1 (MQ=255)                                              gCGGTTTCCCTGCAACGTGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGTGTTTCGCCGTAGGCTTGCGATTATAAGc                                                                                             <  2:1757439/150‑1 (MQ=255)                                              gCGGTTTCCCTGCAACGTGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGc                                                                                             <  1:802742/150‑1 (MQ=255)                                                 gTTTCCCTGCAACGTGCCATTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGc                                                                                                           <  1:1829687/133‑1 (MQ=255)                                                 gTTTCCCTGCAACGTGCCATTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGc                                                                                                           >  2:1829687/1‑133 (MQ=255)                                                        tGCAACGTGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCggg                                                                                                                        >  1:763246/1‑113 (MQ=255)                                                        tGCAACGTGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCggg                                                                                                                        <  2:763246/113‑1 (MQ=255)                                                        tGCAACGTGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGa                                                                                                     <  2:264388/132‑1 (MQ=255)                                                        tGCAACGTGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGa                                                                                                     >  1:264388/1‑132 (MQ=255)                                                        tGCAACGTGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATc                                                                                   <  1:521871/150‑1 (MQ=255)                                                               tGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGa                                                                            <  1:525479/150‑1 (MQ=255)                                                                gCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAg                                                                           <  2:1780101/150‑1 (MQ=255)                                                                  cGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGATTGCGATTATGAGCCCACCAGATCAAGACGAGGc                                                                         >  1:1473379/1‑150 (MQ=255)                                                                   gTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAAAGGCAGGGG‑GAACGCCCCTGCCGTGGGATTACTCAGGCGGGTTTCGCCGTAGGGTGGCGATTATAAGCCTGCCGGGGCAAGACGAGGcc                                                                        >  1:280202/1‑150 (MQ=255)                                                                         aaTGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGAc                                                                  >  1:170350/1‑150 (MQ=255)                                                                          aTGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTgg                                                                                                                                       >  2:1387176/1‑80 (MQ=255)                                                                          aTGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTgg                                                                                                                                       <  1:1387176/80‑1 (MQ=255)                                                                               gTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGa                                                                                                     <  1:1505426/109‑1 (MQ=255)                                                                               gTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGa                                                                                                     >  2:1505426/1‑109 (MQ=255)                                                                               gTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTatcat                                                            >  2:240787/1‑150 (MQ=255)                                                                                tCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTatcatc                                                           <  1:1178698/150‑1 (MQ=255)                                                                                           gTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACGAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAg                                                >  1:803673/1‑150 (MQ=255)                                                                                           gTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTa                                                                <  2:602316/134‑1 (MQ=255)                                                                                           gTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTa                                                                >  1:602316/1‑134 (MQ=255)                                                                                           gTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAg                                                >  2:1282179/1‑150 (MQ=255)                                                                                           gTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAg                                                >  1:1586598/1‑150 (MQ=255)                                                                                               aGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGTGATTATAAGCCCACCAGATCAAGACGAGGCCACCGAATATCATCGGAATGCACAGCAAc                                            >  1:312800/1‑150 (MQ=255)                                                                                               aGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAAc                                            >  1:1315275/1‑150 (MQ=255)                                                                                                gATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACt                                           <  2:881408/150‑1 (MQ=255)                                                                                                gATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACt                                           <  1:573343/150‑1 (MQ=255)                                                                                                gATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACt                                           >  1:1283722/1‑150 (MQ=255)                                                                                                 aTACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAg                                                                                              >  1:725836/1‑98 (MQ=255)                                                                                                 aTACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAg                                                                                              <  2:725836/98‑1 (MQ=255)                                                                                                 aTACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACTg                                          <  1:242019/150‑1 (MQ=255)                                                                                                      gTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAg                                                <  2:1933449/139‑1 (MQ=255)                                                                                                      gTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAg                                                >  1:1933449/1‑139 (MQ=255)                                                                                                      gTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACTGGCCtt                                     <  2:170350/150‑1 (MQ=255)                                                                                                       tttCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACTGGCCTtg                                    >  2:676657/1‑150 (MQ=255)                                                                                                           aTGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACTGGCCTtgagtg                                >  1:41916/1‑150 (MQ=255)                                                                                                                ttACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGt                                                                                                                                         <  1:357595/40‑1 (MQ=255)                                                                                                                ttACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGt                                                                                                                                         >  2:357595/1‑40 (MQ=255)                                                                                                                 tACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACTGGCCTTGAGTGagccag                          <  2:1586598/150‑1 (MQ=255)                                                                                                                  aCCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACTGGCCTTGAGTGagccagc                         >  2:384198/1‑150 (MQ=255)                                                                                                                    cTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGcaca                                                 >  2:1408466/1‑124 (MQ=255)                                                                                                                    cTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGcaca                                                 <  1:1408466/124‑1 (MQ=255)                                                                                                                    cTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACTGGCCTTGAGTGAGCCAGCCg                       >  1:879253/1‑150 (MQ=255)                                                                                                                     tGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACTGGCCTTGAGTGAGCCAGCCGa                      >  1:1432498/1‑150 (MQ=255)                                                                                                                          cAAAACCGGCAGGGG‑GATCTCCCCTGCCGTGGTATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGACGCCACCGACTATCATCGGAATGCACAGCAACTGGCCTTGAGTGAGCCAGCCGAAcgcg                 <  2:41916/150‑1 (MQ=255)                                                                                                                          cAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGca                                                   >  1:1882133/1‑116 (MQ=255)                                                                                                                          cAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGca                                                   <  2:1882133/116‑1 (MQ=255)                                                                                                                           aaaaaCGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACTGGCCTTGAGTGAGCCAGCCGAACGCGa                >  1:199224/1‑150 (MQ=255)                                                                                                                             ccaCGGCAGGGGCGATC‑CCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACTGGCCTTGAGTGagccagc                         >  1:1844568/3‑139 (MQ=255)                                                                                                                             ccaCGGCAGGGGCGATC‑CCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACTGGCCTTGAGTGagccagc                         <  2:1844568/137‑1 (MQ=255)                                                                                                                             aaaCGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACTGGCCTTGAGTGAGCCAGCCGAACGCGATg              <  2:434174/150‑1 (MQ=255)                                                                                                                               aCGGCAGGGGCGATC‑CCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCAc                                                                      <  2:1162552/92‑1 (MQ=39)                                                                                                                               aCGGCAGGGGCGATC‑CCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCAc                                                                      >  1:1162552/1‑92 (MQ=39)                                                                                                                                    aGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACTGGCCTtgagtgag                              >  2:96582/1‑127 (MQ=255)                                                                                                                                    aGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACTGGCCTtgagtgag                              <  1:96582/127‑1 (MQ=255)                                                                                                                                    aGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACTGGCCTTGAGTGagccagc                         <  2:354986/132‑1 (MQ=255)                                                                                                                                    aGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACTGGCCTTGAGTGagccagc                         >  1:354986/1‑132 (MQ=255)                                                                                                                                    aGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACTGGCCTTGAGTGAGCCAGCCGAACGCGATGTAACCGa       <  2:1283722/150‑1 (MQ=255)                                                                                                                                       gg‑gATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACTGGCCTTGAGTGAGCCAGCCGAACGCGATGTAACCGAGtt    >  2:258019/1‑150 (MQ=255)                                                                                                                                          gATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACTGGCCTTGAGTGAGCCAGCCGAACGCGATGTAACCGAGTtgt  <  1:676657/150‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                  GAAACGCAGCATCGCGCCCGGCAGGCTGATGGTGCGGATGCCGGTGCGGTTTCCCTGCAACGTGCCGTTATCAATGACGTCGCGGACCAGGTCCAGATACTGTTTCATGGCTTACCTGTAGCAAAAACGGCAGGGG‑GATCGCCCCTGCCGTGGGATTAATCAGGCGGGTTTCGCCGTAGGCTTGCGATTATAAGCCCACCAGATCAAGACGAGGCCACCGACTATCATCGGAATGCACAGCAACTGGCCTTGAGTGAGCCAGCCGAACGCGATGTAACCGAGTTGT  >  NC_002947/5867654‑5867939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑