| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,574,271 | Δ1 bp | 6.2% | coding (899/2154 nt) | glgX → | glycogen debranching enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,574,271 | 0 | G | . | 6.2% | 205.9 / 7.8 | 64 | coding (899/2154 nt) | glgX | glycogen debranching enzyme |
| Reads supporting (aligned to +/- strand): ref base G (29/31); new base . (2/2); total (31/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
CGCGCTACCTGGCCAGCGGCAAGATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTGGACCTGAGCCACCCCTGCGTGCTGCAGTTGGTCACCGACTCGCTTCGCTACTGGGCCGG > NC_002947/4574130‑4574415 | cgcgCTACCTGGCCAGCGGCAAGATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCg > 2:1100539/1‑150 (MQ=255) cTGGCCAGCGGCAAGATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACCACGcc > 2:660917/1‑150 (MQ=255) ccAGCGGCAAGATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGt > 1:1370824/1‑150 (MQ=255) ccAGCGGCAAGATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGt > 2:581920/1‑150 (MQ=255) cAGCGGCAAGATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGta > 2:1765049/1‑150 (MQ=255) cAGCGGCAAGATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGC‑CGCGCATCGACagg < 1:1640411/142‑3 (MQ=255) cAGCGGCAAGATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGC‑CGCGCATCGACagg > 2:1640411/1‑140 (MQ=255) cAGCGGCAAGATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGGCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGta > 1:1917928/1‑150 (MQ=255) gATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCt < 1:843211/150‑1 (MQ=255) tCGCCGAGTTCAAGGAGATGGTCGCGCACCTTCACTACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGa < 2:1852506/150‑1 (MQ=255) tCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGa < 2:561205/150‑1 (MQ=255) ccGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGa < 2:1029383/124‑1 (MQ=255) ccGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGa > 1:1029383/1‑124 (MQ=255) gTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGga < 1:1488966/150‑1 (MQ=255) gTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGga < 1:1915003/150‑1 (MQ=255) aaGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGgacgac > 1:977265/1‑150 (MQ=255) ggAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACaa < 1:581920/150‑1 (MQ=255) aTGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAgcgc < 1:1765049/150‑1 (MQ=255) gcgcACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCtactac > 1:135333/1‑150 (MQ=255) cTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGTG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCTGACGACAAGCGCTACTACATCAAc < 2:779065/150‑1 (MQ=255) cTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAAc < 2:1845587/150‑1 (MQ=255) cTGCACGACGCGGGGCTTGAGGTGACCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAAc < 1:571148/150‑1 (MQ=255) gCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGa < 1:1186163/150‑1 (MQ=255) gCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGa > 1:810188/1‑150 (MQ=255) cgcgGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCGCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTccgg < 1:224511/150‑1 (MQ=255) ggggCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTccggca‑c > 2:1554857/1‑150 (MQ=255) ttGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCg > 2:640453/1‑92 (MQ=255) ttGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCg < 1:640453/92‑1 (MQ=255) tGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCaa < 2:1917928/150‑1 (MQ=255) gAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAac > 2:1721204/1‑150 (MQ=255) tGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACAccc < 2:1748267/150‑1 (MQ=255) tGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGC‑CGCGCATCGACag < 1:1656935/79‑2 (MQ=38) tGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGC‑CGCGCATCGACag > 2:1656935/1‑78 (MQ=38) tCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTgg < 2:128597/150‑1 (MQ=255) ccTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTGGa < 1:1554857/150‑1 (MQ=255) gACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTGGACCTg > 1:78293/1‑150 (MQ=255) aCGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTGGACCTGa < 1:1721204/150‑1 (MQ=255) aCGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTGGACCTGa < 2:1370824/150‑1 (MQ=255) gTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACaa < 2:849826/68‑1 (MQ=255) gTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACaa > 1:849826/1‑68 (MQ=255) cTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTGGACCTGAGCCAccc < 2:78293/150‑1 (MQ=255) cAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTg < 1:739645/133‑1 (MQ=255) cAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTg > 2:739645/1‑133 (MQ=255) aCCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTGGACCTGAGCCACCCCTGCg > 2:652578/1‑150 (MQ=255) ccACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTAATGCCGGACGACAAGCGCTACTACATCACAGACTCCGGCA‑CCGGCAACACCCTGGACCTGAGCCACCCCTGCGt > 1:128175/1‑150 (MQ=255) cacaCCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTc < 1:491440/110‑1 (MQ=255) cacaCCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTc > 2:491440/1‑110 (MQ=255) cacaCCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTGGACCt < 1:216314/134‑1 (MQ=255) cacaCCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTGGACCt > 2:216314/1‑134 (MQ=255) cacaCCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTGGACCTGAGCCACCCCTGCGtg > 1:1931144/1‑150 (MQ=255) acacCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTGGACCTGAGCCACCCCTGCGtgc > 1:1839132/1‑150 (MQ=255) cacCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTGGACCTGAGCCACCCCTGCGtgct < 2:1931144/150‑1 (MQ=255) gccgAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACAcc < 2:649935/120‑1 (MQ=255) gccgAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACAcc > 1:649935/1‑120 (MQ=255) gAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTGGACCTGAGCCACCCCTGCGTGCTGCAGTTg < 2:135333/150‑1 (MQ=255) gCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGTCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATAAACGAAT‑CGACAGCCGGCAACACCCTGGACCTGAGGCACCCCCACGTGCTGCAGTGGGTCa > 1:1574439/1‑150 (MQ=255) cGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACATGGTCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTGGACCTGAGCCACCCCTGCGTGCTGCAGTTGGTCACCGa < 1:511372/150‑1 (MQ=255) gAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCATTCACCTGAGCCACCCCTGCGTGCTGCAGTTGGTCACCGAc > 1:1774012/1‑150 (MQ=255) gACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTGGACCTGAGCCACCCCTGAGTGCCGGGGTTGGTCACCGACTCGCTTCGCTa > 1:493142/1‑150 (MQ=255) aCCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAAc < 2:403431/75‑1 (MQ=255) aCCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAAc > 1:403431/1‑75 (MQ=255) gTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTgg < 1:1298597/95‑1 (MQ=255) gTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTgg > 2:1298597/1‑95 (MQ=255) gATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTGGACCTGAGCCACCCCTGCGTGCTGCAGTTGGTCACCGACTCGCTTCGCTACTGGGCCgg > 1:651312/1‑150 (MQ=255) | CGCGCTACCTGGCCAGCGGCAAGATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCG‑GCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCA‑CCGGCAACACCCTGGACCTGAGCCACCCCTGCGTGCTGCAGTTGGTCACCGACTCGCTTCGCTACTGGGCCGG > NC_002947/4574130‑4574415 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |