Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,866,753	A→C	10.8%	intergenic (+20/+37)	PP_5140 → / ← thyA	MerR family transcriptional regulator/thymidylate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,866,753	0	A	C	10.8%	161.5 / 10.6	64	intergenic (+20/+37)	PP_5140/thyA	MerR family transcriptional regulator/thymidylate synthase
Reads supporting (aligned to +/- strand):  ref base A (28/29);  new base C (3/4);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

GTGGAGCTGCGGCGGCGCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCAGTTTCGG  >  NC_002947/5866605‑5866899                                                                                                                                                     |                                                                                                                                                   gtggAGCTGCGGCGGCGCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGaa                                                                                                                                                   >  2:243731/1‑150 (MQ=255)  tggAGCTGCGGCGGCGCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGCACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAAc                                                                                                                                                  <  1:442024/150‑1 (MQ=255)        tGCGGCGGCGCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACAACGACGTCTCCGTACTGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTTCACCCGCGa                                                                                                                                            <  1:1690291/150‑1 (MQ=255)         gcggcggcgCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGaa                                                                                                                                           >  1:1747878/1‑150 (MQ=255)              ggcgCGGAAACGAGCAGGGGAGGGAATGCGCGATCTTGCAGCAACTGGCGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTTCACCCGCGAagcttt                                                                                                                                      <  2:944876/150‑5 (MQ=255)                   tGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAagag                                                                                                                                        <  1:936995/143‑1 (MQ=255)                   tGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAagag                                                                                                                                        >  2:936995/1‑143 (MQ=255)                   tGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGtt                                                                                                                                 >  2:722522/1‑150 (MQ=255)                    gCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTTGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTc                                                                                                                                <  2:459147/150‑1 (MQ=255)                       aCGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTaa                                                                                                                             <  2:1886352/150‑1 (MQ=255)                        cgcgCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAAc                                                                                                                            <  2:1788067/150‑1 (MQ=255)                        cgagcAGGGGAGTGAATGCGAGATCTTGCAGGAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAAc                                                                                                                            <  2:112362/147‑1 (MQ=255)                             aGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACTGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATag                                                                                                                       <  1:1828211/150‑1 (MQ=255)                              ggggAGTGAATGCGCGATCTTGCAGAAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATaga                                                                                                                      <  2:77151/150‑1 (MQ=255)                                 gAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGACCCCGCGAAGAGGTCAGTTCTAACAATAGAGAt                                                                                                                   <  1:1110819/150‑1 (MQ=255)                                   gTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCt                                                                                                                 >  2:1116076/1‑150 (MQ=255)                                         gcgcGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGa                                                                                                           >  2:1471390/1‑150 (MQ=255)                                          cgcgATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGAc                                                                                                          >  1:1195505/1‑150 (MQ=255)                                           gcgATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATaga                                                                                                                      >  1:1806063/1‑137 (MQ=255)                                           gcgATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATaga                                                                                                                      <  2:1806063/137‑1 (MQ=255)                                           gcgATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGAcc                                                                                                         >  2:1160350/1‑150 (MQ=255)                                                 ttGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATc                                                                                                   <  1:1625019/150‑1 (MQ=255)                                                    cagcaACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGgc                                                                                                <  2:1195505/150‑1 (MQ=255)                                                       caACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCt                                                                                             <  1:1471390/150‑1 (MQ=255)                                                        aaCTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTg                                                                                            >  1:1219859/1‑150 (MQ=255)                                                        aaCTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTg                                                                                            >  1:888974/1‑150 (MQ=255)                                                          cTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTc                                                                                          <  1:449110/150‑1 (MQ=255)                                                          cTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTc                                                                                          <  1:1116076/150‑1 (MQ=255)                                                                  aaaCGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAagag                                                                                                                                        >  1:1510504/1‑96 (MQ=255)                                                                  aaaCGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAagag                                                                                                                                        <  2:1510504/96‑1 (MQ=255)                                                                  aaaCGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGgcgc                                                                                  >  2:1003834/1‑150 (MQ=255)                                                                   aaaggggCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCa                                                                                 >  1:1934154/4‑150 (MQ=255)                                                                    aCGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCAt                                                                                <  1:243731/150‑1 (MQ=255)                                                                    aCGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCAt                                                                                <  2:1219859/150‑1 (MQ=255)                                                                       gggCGGTACCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGt                                                                             >  2:736904/1‑150 (MQ=255)                                                                        ggCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTg                                                                            >  1:906586/1‑150 (MQ=255)                                                                                  gTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCt                                                                  >  1:1078876/1‑150 (MQ=255)                                                                                     ccGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTcc                                                               >  2:759393/1‑150 (MQ=255)                                                                                       ggATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTccag                                                             >  2:843860/1‑150 (MQ=255)                                                                                        gATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGcc                                                                                                      <  2:1751854/108‑1 (MQ=255)                                                                                        gATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGcc                                                                                                      >  1:1751854/1‑108 (MQ=255)                                                                                                  aTTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGATCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCAc                                                  >  1:506396/1‑150 (MQ=255)                                                                                                       ccgctctggCGGAGTCACGGGCATTGAGCCGACTCCTTGGAGGGTGCCCCATCTCCGACTTCAGGGCCAACAAGAGATATCGATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGc                                             <  2:425833/143‑1 (MQ=255)                                                                                                        aggttgggCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCt                                            <  1:1160350/145‑1 (MQ=255)                                                                                                           tgGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCaa                                         <  2:888974/150‑1 (MQ=255)                                                                                                            ggggCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAAt                                        <  2:1747878/150‑1 (MQ=255)                                                                                                             gggCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTccagcc                                                           <  2:668921/130‑1 (MQ=255)                                                                                                             gggCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTccagcc                                                           >  1:668921/1‑130 (MQ=255)                                                                                                             gggCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAAtc                                       >  2:480124/1‑150 (MQ=255)                                                                                                               gCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGCAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAAtctt                                     <  1:143266/150‑1 (MQ=255)                                                                                                               gCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCtagatcgg                                     >  1:425833/1‑143 (MQ=255)                                                                                                                  gAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTccagcca                                                          >  1:158475/1‑126 (MQ=255)                                                                                                                  gAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTccagcca                                                          <  2:158475/126‑1 (MQ=255)                                                                                                                    gTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCa                                <  1:759393/150‑1 (MQ=255)                                                                                                                       aCGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGcc                             >  2:195107/1‑150 (MQ=255)                                                                                                                        cGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCa                            >  2:1212842/1‑150 (MQ=255)                                                                                                                                   ccAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCg                       <  2:394280/144‑1 (MQ=255)                                                                                                                                   ccAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCg                       >  1:394280/1‑144 (MQ=255)                                                                                                                                        ctcTTCGCGGGTTCACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCCTGCCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTg            >  1:138316/3‑150 (MQ=255)                                                                                                                                        ctcTTCGCGGGTTCACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGgc                                                                                    <  1:1571197/76‑1 (MQ=38)                                                                                                                                        ctcTTCGCGGGTTCACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGgc                                                                                    >  2:1571197/3‑78 (MQ=38)                                                                                                                                           ttCGCGGGTTCACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCa         >  1:1461432/1‑150 (MQ=255)                                                                                                                                           ttCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCGTGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCa         <  2:1083968/150‑1 (MQ=255)                                                                                                                                              gcgGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCAGtt      >  1:534461/1‑150 (MQ=255)                                                                                                                                                  gTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCAGTTTCgg  <  1:1869606/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GTGGAGCTGCGGCGGCGCTGCAACGCGCAGGGGAGTGAATGCGCGATCTTGCAGCAACTGGAGACAAACGGGGCGGTATCGGTACCGGATACCGAACATTCCCATGTGGGGCGGAGTCACGGGCATTGAGCCAAAAGCTTCGCGGGTGAACCCGCGAAGAGGTCAGTTCTAACAATAGAGATCTATCAGACCGCCATCGGCGCTGTCATCGGCGCATGGTGCTCATAGCCTTCCAGCCAGAAATCACTCGGCTCAATCTTCTCCAGCCACTCCGGCTCATACTTGCCAGTTTCGG  >  NC_002947/5866605‑5866899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑