Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,104,109 T→A 7.7% intergenic (+31/‑83) PP_5353 → / → PP_5354 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,104,1090TA 7.7% 314.4 / 15.4 104intergenic (+31/‑83)PP_5353/PP_5354hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (45/51);  new base A (5/3);  total (50/54)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.38e-01

Only 100 of 105 total aligned reads displayed.

CGTGTCGAAGAATTCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCGGTGCCCCTGGAGCTTCC  >  NC_002947/6103961‑6104256
                                                                                                                                                    |                                                                                                                                                   
cGTGTCGAAGAATTCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCtt                                                                                                                                                    <  1:897987/150‑1 (MQ=255)
      gaagaaTTCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCgg                                                                                                                                              >  1:359067/1‑150 (MQ=255)
       aagaaTTCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGGCCggg                                                                                                                                             <  2:688251/150‑1 (MQ=255)
         gaaTTCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGt                                                                                                                                           >  2:1091257/1‑150 (MQ=255)
           aTTCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCggg                                                                                                                                             >  1:421813/1‑146 (MQ=255)
           aTTCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCggg                                                                                                                                             <  2:421813/146‑1 (MQ=255)
              cgcgCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAAAACCCCGGGCAAGCCCGGGGttctgt                                                                                                                                      <  2:1081495/150‑5 (MQ=255)
               gcgcTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTttttgc                                                                                                                                     >  2:1890506/1‑150 (MQ=255)
                  cTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTACCCGTCAAAACAGCTAATCGCTGCGTGCTAACCACAGAACCCAGGTCTTGCCCGGAGTtgttgcttt                                                                                                                                  <  2:1574884/150‑1 (MQ=255)
                   ttCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCTGGCAAGCCCGGGGttc                                                                                                                                         >  2:1298021/1‑141 (MQ=255)
                   ttCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCTGGCAAGCCCGGGGttc                                                                                                                                         <  1:1298021/142‑2 (MQ=255)
                   ttCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTttttgctttt                                                                                                                                 >  1:1938493/1‑150 (MQ=255)
                   ttCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTttttgctttt                                                                                                                                 <  1:471109/150‑1 (MQ=255)
                         cAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCaaa                                                                                                                           >  1:1858474/1‑150 (MQ=255)
                           ggtcGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGt                                                                                                                         <  1:439906/147‑1 (MQ=255)
                            ggCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTg                                                                                                                        >  2:894140/1‑150 (MQ=255)
                            ggCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTg                                                                                                                        >  1:1866109/1‑150 (MQ=255)
                             gCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTttttgctttt                                                                                                                                 >  2:1440816/1‑140 (MQ=255)
                             gCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTttttgctttt                                                                                                                                 <  1:1440816/140‑1 (MQ=255)
                              cGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGAGGCGGTGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTggg                                                                                                                      <  1:1556444/150‑1 (MQ=255)
                               gAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGc                                                                                                                     <  1:1215687/150‑1 (MQ=255)
                               gAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGc                                                                                                                     <  2:1938493/150‑1 (MQ=255)
                                     ttCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAg                                                                                                               >  2:1648396/1‑150 (MQ=255)
                                      tCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTACTCGCTGCGTGCTACACCCAGAACCCTGTGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAgg                                                                                                              <  1:1694495/150‑1 (MQ=255)
                                       cAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAggg                                                                                                             <  1:1735910/150‑1 (MQ=255)
                                        aGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCTGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGt                                                                                                            >  2:1152559/1‑150 (MQ=255)
                                          cGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGtat                                                                                                          <  1:1091257/150‑1 (MQ=255)
                                            aaaGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGctact                                                                                                                 <  2:1519264/141‑1 (MQ=255)
                                            aaaGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGctact                                                                                                                 >  1:1519264/1‑141 (MQ=255)
                                                 tGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATaa                                                                                                        <  1:1879901/145‑1 (MQ=255)
                                                 tGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATaa                                                                                                        >  2:1879901/1‑145 (MQ=255)
                                                       aTGGTTCGCGCCCAAGGCGAGCACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGtt                                                                                             >  2:664385/1‑150 (MQ=255)
                                                       aTGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGtt                                                                                             >  1:279389/1‑150 (MQ=255)
                                                        tGGTTCTCGCCTTGGGCGCGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTg                                                                                            >  2:1664143/1‑150 (MQ=255)
                                                          gTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTc                                                                                          <  1:664385/150‑1 (MQ=255)
                                                             cgcgCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCggg                                                                                                                                             >  2:1663975/1‑96 (MQ=255)
                                                             cgcgCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCggg                                                                                                                                             <  1:1663975/96‑1 (MQ=255)
                                                             cgcgCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATc                                                                                       >  2:1612133/1‑150 (MQ=255)
                                                             cgcgCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATc                                                                                       <  2:279389/150‑1 (MQ=255)
                                                             cgcgCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATc                                                                                       <  1:945485/150‑1 (MQ=255)
                                                                gcCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAg                                                                                    >  1:636442/1‑150 (MQ=255)
                                                                   cAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTc                                                                                 >  1:721881/1‑150 (MQ=255)
                                                                      ggCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCaga                                                                              <  1:894140/150‑1 (MQ=255)
                                                                       gCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCagag                                                                             <  2:1771214/150‑1 (MQ=255)
                                                                       gCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCagag                                                                             >  2:1492612/1‑150 (MQ=255)
                                                                         gagaACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGa                                                                           >  1:1017025/1‑150 (MQ=255)
                                                                          agaACCAGCCTTCGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGaa                                                                          <  1:1857760/150‑1 (MQ=255)
                                                                           gaACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCTGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAg                                                                         <  1:1152559/150‑1 (MQ=255)
                                                                              ccAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGa                                                                      >  1:414420/1‑150 (MQ=255)
                                                                               cAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGTTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGaa                                                                     >  2:1716157/1‑150 (MQ=255)
                                                                               cAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGaa                                                                     >  2:1722827/1‑150 (MQ=255)
                                                                                   cTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGt                                                                 <  1:1890506/150‑1 (MQ=255)
                                                                                    ttGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTg                                                                <  2:1858474/150‑1 (MQ=255)
                                                                                           gTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTCCTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGaagaag                                                         >  2:263265/1‑150 (MQ=255)
                                                                                           gTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGaagaag                                                         >  1:1091973/1‑150 (MQ=255)
                                                                                            tAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACGCAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAg                                                                                    >  2:569013/1‑122 (MQ=255)
                                                                                            tAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACGCAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAg                                                                                    <  1:569013/122‑1 (MQ=255)
                                                                                              gCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGa                                                      <  2:1563311/150‑1 (MQ=255)
                                                                                               ccGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGAt                                                     <  2:359067/150‑1 (MQ=255)
                                                                                                cGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATc                                                    <  1:251577/150‑1 (MQ=255)
                                                                                                 ggTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCg                                                   <  2:1032802/150‑1 (MQ=255)
                                                                                                 ggTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCg                                                   <  1:1664143/150‑1 (MQ=255)
                                                                                                   tATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATc                                                    >  2:82952/1‑147 (MQ=255)
                                                                                                   tATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATc                                                    <  1:82952/147‑1 (MQ=255)
                                                                                                   tATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGaa                                                 <  2:1091973/150‑1 (MQ=255)
                                                                                                   tATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACAGTGGAAGAAGTGATCGaa                                                 >  2:1325893/1‑150 (MQ=255)
                                                                                                   tATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCCCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGaa                                                 <  1:213659/150‑1 (MQ=255)
                                                                                                   tATCCGTCAAAACAGCTAATCGCTGCGTACTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGAt                                                     >  1:1803105/1‑146 (MQ=255)
                                                                                                   tATCCGTCAAAACAGCTAATCGCTGCGTACTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGAt                                                     <  2:1803105/146‑1 (MQ=255)
                                                                                                    aTCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGACCCCAGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGTGCTGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGaac                                                <  1:1504829/150‑1 (MQ=255)
                                                                                                    aTCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAg                                                                                    <  2:887155/114‑1 (MQ=255)
                                                                                                    aTCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAg                                                                                    >  1:887155/1‑114 (MQ=255)
                                                                                                        gTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGaa                                                                          <  2:1765283/120‑1 (MQ=255)
                                                                                                          cAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTcc                                          <  2:636442/150‑1 (MQ=255)
                                                                                                          cAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTcc                                          <  2:636438/150‑1 (MQ=255)
                                                                                                             aaCAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGtt                                                                                             >  1:810358/1‑96 (MQ=255)
                                                                                                             aaCAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGtt                                                                                             <  2:810358/96‑1 (MQ=255)
                                                                                                                  cTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCg                                  >  1:366242/1‑150 (MQ=255)
                                                                                                                   tAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGt                                                                 >  2:1695606/1‑118 (MQ=255)
                                                                                                                   tAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGt                                                                 <  1:1695606/118‑1 (MQ=255)
                                                                                                                         cTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGc                           <  1:1648396/150‑1 (MQ=255)
                                                                                                                           gCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGCTTAACTCCGTGAAGCGAATGAGCCg                         <  1:263265/150‑1 (MQ=255)
                                                                                                                           gCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCg                         <  2:1017025/150‑1 (MQ=255)
                                                                                                                                cTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGaagaag                                                         <  2:302014/113‑1 (MQ=255)
                                                                                                                                cTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGaagaag                                                         >  1:302014/1‑113 (MQ=255)
                                                                                                                                 tAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCg                   <  1:1612133/150‑1 (MQ=255)
                                                                                                                                     gcaaaaaCCCCGGGCAAGCCCGGGGTTCTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCGGTGc               >  1:147660/6‑150 (MQ=255)
                                                                                                                                      acaGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCgg                        >  1:660930/1‑140 (MQ=255)
                                                                                                                                      acaGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCgg                        <  2:660930/140‑1 (MQ=255)
                                                                                                                                       aaaaaCCCCGGGCAAGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGaa                                >  1:1375956/4‑131 (MQ=255)
                                                                                                                                       aaaaaCCCCGGGCAAGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGaa                                <  2:1375956/128‑1 (MQ=255)
                                                                                                                                         gAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCGGTGCCCCt           <  2:721881/150‑1 (MQ=255)
                                                                                                                                         gAACCCCGGGCTTGCACGGAGTTTTTCTATTTCGCAAGGTGTTCTACTAGACTATCAGCCGTACCCTTCGCCGCTAGATCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCGGTGCCCCt           <  1:1492612/150‑1 (MQ=255)
                                                                                                                                          aaCCCCGGGCAAGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCGGTGCCCCTg          >  1:870716/1‑150 (MQ=255)
                                                                                                                                            ccccGGGCTTGCCCGGGGTTTTTGCTTTTTTTTTTTTTTTCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCGGTGCCCCTGGa        >  2:1902180/1‑150 (MQ=255)
                                                                                                                                            ccccGGGCAAGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCGGTGCCCCTGGa        >  1:473031/1‑150 (MQ=255)
                                                                                                                                             cccGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCGGTGCCCCTGGAg       >  2:348925/1‑150 (MQ=255)
                                                                                                                                             cccGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCGGTGCCCCTGGAg       >  2:914882/1‑150 (MQ=255)
                                                                                                                                                gggCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCGGTGCCCCTGGAGCtt    >  2:515440/1‑150 (MQ=255)
                                                                                                                                                  gCTTGCCGTGGGTTTTTGCTTTTGCCAAATTGGGCTACTAGGTGATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACCCCGTGAAGCGAATGAGCCGGTACCGGTGCCCCTGGAGCTTcc  <  2:717243/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGTGTCGAAGAATTCGCGCTTCGACCAGGGCGAAGTGTTCAGCGAAAGCTGGCACATGGTTCGCGCCCAAGGCGAGAACCAGCCTTGGCTGGTAGCCGGTATCCGTCAAAACAGCTAATCGCTGCGTGCTAAACACAGAACCCCGGGCTTGCCCGGGGTTTTTGCTTTTGCCAAAGTGGGCTACTAGGGTATAAGGCGCAGCGTTGTCATCTAGGTCAGAGGAAGTGAACCGTGGAAGAAGTGATCGAACAACTCCGTGAAGCGAATGAGCCGGTACCGGTGCCCCTGGAGCTTCC  >  NC_002947/6103961‑6104256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑