Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,140,036 G→T 9.5% K395N (AAG→AAT)  cusB → CzcB family copper RND transporter membrane fusion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,140,0360GT 9.5% 119.3 / 3.3 42K395N (AAG→AAT) cusBCzcB family copper RND transporter membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base G (21/17);  new base T (2/2);  total (23/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01

TCGCCGAAGACCAAGGCCGTTTCCGCCCAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCACCTTGGGAATGC  >  NC_002947/6139888‑6140181
                                                                                                                                                    |                                                                                                                                                 
tCGCCGAAGACCAAGGCCGTTTCCGCCCAATAGAGGTGGTGCTAGGCCAGGAGCCCCACCCGCGCCGAGCATTCGGTAGCGGCCTGCATGCCGGGCAGAACGTCGTCACACCCGTGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAgg                                                                                                                                                  <  1:1407089/150‑1 (MQ=255)
tCGCCGAAGACCAAGGCCGTTTCCGCCCAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAgg                                                                                                                                                  <  2:1065330/150‑1 (MQ=255)
tCGCCGAAGACCAAGGCCGTTTCCGCCCAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAgg                                                                                                                                                  >  2:1075130/1‑150 (MQ=255)
tCGCCGAAGACCAAGGCCGTTTCCGCCCAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAgg                                                                                                                                                  >  1:305891/1‑150 (MQ=255)
        gACCAAGGCCGTTTCCGCCCAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGAGCGACTCCGAAGCAAGCCTCAAGGGTAttgag                                                                                                                                          >  1:640658‑M2/1‑145 (MQ=255)
        gACCAAGGCCGTTTCCGCCCAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCAACGGGGCAATTCCTGATCGACTCCGAAGCAAGCCGCAAGGGTAttgag                                                                                                                                          >  1:449744‑M2/1‑145 (MQ=255)
           cAAGGCCGTTTCCGCCCAATAGAGGTGGTGCTAGGCCAGGAGAAAGAAGTTTAAGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGCAAATCGTGGCATTCCGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGTTATTGAGGcc                                                                                                                                       >  1:1806805/1‑150 (MQ=255)
             aGGCCGTTTCCGCCCAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGccac                                                                                                                                     <  1:1280753/150‑1 (MQ=255)
              ggCCGTTTCCGCCCAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGccac                                                                                                                                     >  1:475217/1‑149 (MQ=255)
              ggCCGTTTCCGCCCAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGccac                                                                                                                                     <  2:475217/149‑1 (MQ=255)
                      ccGCCCAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATc                                                                                                                            <  1:1794022/150‑1 (MQ=255)
                      atctccAATATAGGTCGTGCTAGCCCAGGAGAACGGCTGCCACTTCTCAATCTTCAAAGGCCTGCAGGCCGGGCAGAAACTCGTGGCATCCTTGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATc                                                                                                                            <  1:927338/146‑1 (MQ=255)
                         cccAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGa                                                                                                                         >  1:302978/1‑150 (MQ=255)
                         cccAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGa                                                                                                                         <  2:656930/150‑1 (MQ=255)
                         cccAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGa                                                                                                                         <  1:1075130/150‑1 (MQ=255)
                           cAATAGAGGTGGTGCTAGGCAAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGGCTGCAGGCCGGGCAGAAAGTCGTGGCATCAGGGCAATTCCTGATCGACTCCGAAGAAAGACTCAAGGGTATTGAGGCCACAAGAGCAtagagat                                                                                                                       >  2:927338/1‑144 (MQ=255)
                             aTAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCa                                                                                                                     >  1:1470994/1‑150 (MQ=255)
                                   gtggtgCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAg                                                                                                                                                   >  2:1244900/1‑114 (MQ=255)
                                   gtggtgCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAg                                                                                                                                                   <  1:1244900/114‑1 (MQ=255)
                                     ggtgCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCgcctgc                                                                                                             <  1:1208491/150‑1 (MQ=255)
                                                      aCGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTACTGAGGCTACCACAGCATCGGTTTCACCGCCTGCCTCGCAAGCACCTGAAc                                                                                            <  2:221627/150‑1 (MQ=255)
                                                        ggcggcCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAAccc                                                                                          >  2:360023/1‑150 (MQ=255)
                                                         gcggcCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGt                                                                                                                                                <  1:1889555/95‑1 (MQ=255)
                                                         gcggcCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGt                                                                                                                                                >  2:1889555/1‑95 (MQ=255)
                                                                  tCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAATACCCttgaggcttgcttcggagtcgatcaggaattgcccggatgccac                                                                                                   >  1:1553627‑M2/1‑87 (MQ=255)
                                                                  tCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAATACCCttgaggcttgcttcggagtcgatcaggaattgcccggatgccac                                                                                                   <  2:1553627‑M2/131‑45 (MQ=255)
                                                                    gCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCAc                                                                                                                    >  1:1202097/1‑112 (MQ=255)
                                                                    gCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCAc                                                                                                                    <  2:1202097/112‑1 (MQ=255)
                                                                          ttGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAATACCCttgaggcttgcttcgga                                                                                                                              >  2:1401226‑M2/1‑79 (MQ=255)
                                                                          ttGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAATACCCttgaggcttgcttcgga                                                                                                                              <  1:1401226‑M2/96‑18 (MQ=255)
                                                                           tGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATg                                                                       <  1:916347/150‑1 (MQ=255)
                                                                                   cTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTAttgag                                                                                                                                          <  1:456940‑M2/75‑6 (MQ=255)
                                                                                   cTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTAttgag                                                                                                                                          >  2:456940‑M2/1‑70 (MQ=255)
                                                                                         gCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGAAAGACTGGCGGGGAGTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACaa                                                         >  2:1719593/1‑150 (MQ=255)
                                                                                                 gAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAgg                                                 <  2:302978/150‑1 (MQ=255)
                                                                                                        gTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAg                                          >  1:162673/1‑150 (MQ=255)
                                                                                                              tCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCgtgt                                                                <  2:350319/122‑1 (MQ=255)
                                                                                                              tCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCgtgt                                                                >  1:350319/1‑122 (MQ=255)
                                                                                                               ccGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCa                                   <  1:1329770/150‑1 (MQ=255)
                                                                                                                           tGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGc                       >  1:90915/1‑150 (MQ=255)
                                                                                                                           tGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGc                       >  2:1857449/1‑150 (MQ=255)
                                                                                                                            gATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAACCGCAGGCACCCAGCTGACCATCGACCACGGGcc                      >  1:499946/1‑150 (MQ=255)
                                                                                                                                caCTCCGAAGAAAGCCTTAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGTACCCATCTTACCATCGACCACGGGCCATTc                  <  2:689036/149‑1 (MQ=255)
                                                                                                                                   tCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTc               >  2:1198425/1‑150 (MQ=255)
                                                                                                                                     cGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCAc             >  1:564805/1‑150 (MQ=255)
                                                                                                                                     cGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCATGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCAc             >  2:1927436/1‑150 (MQ=255)
                                                                                                                                                tCAAGGGTCTTTACGATACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCACCTTGGGAATGc  <  2:1806805/150‑1 (MQ=255)
                                                                                                                                                tCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCACCTTGGGAATGc  >  1:568619/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TCGCCGAAGACCAAGGCCGTTTCCGCCCAATAGAGGTGGTGCTAGGCCAGGAGAACGGCGGCCAGGTCGCAATCTTGAAAGGCCTGCAGGCCGGGCAGAAAGTCGTGGCATCCGGGCAATTCCTGATCGACTCCGAAGCAAGCCTCAAGGGTATTGAGGCCACCACAGCATCGGATTCACCGCCTGCCTCGCAAGCACCTGAACCCGAACTGCACCAAGCGGATGGCCGTGTCGTACAAATCGAAGGCACCCAGCTGACCATCGACCACGGGCCATTCGTCACCTTGGGAATGC  >  NC_002947/6139888‑6140181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑